Objective To explore the role of oval cells in hepatic injure and to study its mechanism.Methods Fifty male SD rats were randomly divided into control group(20 cases)and model group(30 cases).Hepatic pathologic changes were observed through optical microscope and electron microscope.Vimentin and PCNA were detected by immunohistochemistry,Notch1、RBP-J?、HES1 mRNA were detected by semi-quantitative RT-PCR method.Results The histological morphology was normal in the control group,the positive cells of vimentin and PCNA were found on occasion,the expression of Notch1、RBP-J?、HES1 mRNA was low.In the model group,the oval cells were discovered in portal duct regions massively.The tonofilaments and desmosome juntion which were the distinctive structure of oval cells were observed by electron microscopy.The positive cells of vimentin and PCNA were increased(P

Objective To explore the characteristics of gastric myoelectrical activity induced by meal in obese people.Methods Fourty-one cases of obese subjects were investigated with Digitrapper electrogastrography(EGG) before and after test meal,32 healthy volunteers with normal body mass index were matched as control.Results①Before the meal, the percentage of normal slow waves(N%)in obese group was lower than that in control subjects(55.52?15.7 vs 70.83?14.31,P

Objective We aimed to translate the English version of Perceived Control of Asthma Questionnaire (PCAQ) into Chinese and to test the reliability and validity of the Chinese version of PCAQ in asthma patients.Methods Totally 125 patients with asthma were recruited and were investigated by the Chinese version of PCAQ.The results underwent reliability and validity test.Results The Cronbach's α coefficient of the Chinese version of PCAQ ranged from 0.815 to 0.904,higher than 0.7.The split half coefficient was 0.647; the test-retest reliability was 0.818,which showed that internal consistency of Chinese version of PCAQ was good.The content validity index was 0.952.Factor analysis got 3 factors,which explained 46.079％ of the total variance; the factor loading of each item was ＞0.4.Conclusions The Chinese version of PCAQ has been proved to be rehable and valid.It can be used as a valid tool for the measurement of perceived control in asthma patients.

OBJECTIVE:To probe into the status quo and the trend of the utilization of the oral stasis-eliminating Chinese patent medicine in our hospital in order to provide scientific basis for rational use of this medicine.METHODS:The consumption amount and consumption sum of the oral stasis-eliminating Chinese patent medicine in our hospital during 2006-2008 were reviewed in respect of the DDDs and DDC using DDDs ranking method.RESULTS:During 2006～2008,the consumption amount of the oral stasis-eliminating Chinese patent medicine in our hospital had a steady increase,and the DDDs showed a good synchronism.CONCLUSION:The use of stasis-eliminating Chinese patent medicine is rational in our hospital.

Objective:To explore the expression of GITR/GITRL in rheumatoid arthritis(RA)and its correlation with clinical features of the disease.Methods:Fifty three RA patients,35 osteoarthritis(OA)patients and 35 healthy volunteers were included in the study.The real-time-fluorescence quantitative PCR method was used to analyze the expression level of GITR/GITRL mRNA.Using fluorescence-activated cell sorting(FACS),the expression of GITR on CD4+CD25+ T cells in peripheral blood monouuclear cells(PBMC)was detected.The levels of Anti-CCP antibody,C-reactive protein(CRP),erythrocyte sedimentation rate(ESR)and rheumatoid factor(RF)of the RA patients were measured at the same time.Results:It was shown that the level of GITR/GITRL mRNA and the percentage of GITR+ T cells in the CD4+CD25+ T cell subpopulation[12.38?5.72/16.41?10.16,(28.12?16.85)%] were significantly higher than those of the OA group [9.59?5.87/12.09?7.53,(21.01?14.42)%,P

To make a systematic study on acupoints selection and medication rules for allergic rhinitis treated with acupoint application, literature regarding clinical researches on acupoint application for allergic rhinitis since 2000 has been retrieved; medication and acupoints involved were preprocessed and cluster analysis technique was applied to classify the data. As a result, a total of 37 pieces of literature were included, involving 27 types of herbs and 33 acupoints. The results showed Baijiezi (Brassica alba Boiss), Xixin (Radix et Rhizoma Asari), Gansui (Radix Kansui), Yanhusuo (Corydalis), Jiangzhi (Ginger juice) and Baizhi (Radix angelicae) constituted the main herb prescription, and the layer was increased along with frequency of use. Feishu (BL 13), Dazhui (GV 14), Shenshu (BL 23), Pishu (BL 20), Gaohuang (BL 43), Fengmen (BL 12) constituted the main acupoint prescription, which were clustered into 4 classes; Feishu (BL 13) and Dazhui (GV 14) constituted the first class; Pishu (BL 20) and Shenshu (BL 23) constituted the second class; the first and second class merged into the third class, Including Feishu (BL 13), Dazhui (GV 14), Shenshu (BL 23) and Pishu (BL 20); Gaohuang (BL 43) and Fengmen (BL 12) constituted the fourth class; the fourth class and the third class constituted the whole acupoint prescription. It was showed that for the allergic rhinitis treated with acupoint application, the medication was mainly epispastic herbs with pungent nature that belonged to the lung, spleen and kidney meridian, which presented the principle of simultaneous treatment of principal and subordinate symptoms; also the medication can be selected from core layer to supporting layer based on clinical needs; for the selection of acupoints, acupoints in lung and bladder meridian were mainly selected to assist exterior and resist the pathogenic qi, which could treat allergic rhinitis fundamentally by joint use with acupoints in soleen and kidney meridian.
Acupuncture Points ; Data Mining ; Databases, Bibliographic ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Humans ; Meridians ; Rhinitis, Allergic ; drug therapy

Clinical literature of bronchial asthma treated with acupoints application from January 2000 to March 2014 in modern periodicals databases was retrieved through computer. With cluster analysis and frequency analysis methods of data mining, acupoints selecting and medication rules of bronchial asthma treated with acupoints application were analyzed. Total 38 articles were included eventually, including 25 acupoints and 42 medicines. The results indicate that on acupoints selecting, Feishu (BL 13) is used as the main acupoint and 3 groups of bladder meridian and conception vessel acupoints are applied alternately and on medicines, Baijiezi (Brassica alba Boiss), Xixin (Radix et Rhizoma Asari), Gansui (Radix Kansui), Yanhusuo (Corydalis) and Mahuang (Radix et Rhizonma Ephedrae) are primarily adopted, epispastic medicines being the main medicines; medicines mostly belong to lung meridian, main medicines being unchanged mostly with Shengjiang as guiding drug.
Acupuncture Points ; Acupuncture Therapy ; Anti-Asthmatic Agents ; therapeutic use ; Asthma ; drug therapy ; therapy ; Data Mining ; Databases, Factual ; Humans ; Meridians

Objective To determine whether the supplementation of ω-3 polyunsaturated fatty acids in parenteral nutrition (PN) confers treatment benefits to outcomes of septic patients.Methods The databases of MEDLINE,EMBASE,Cochrane central register of controlled trials,the CNKI and the Wanfang biomedicine were searched to identify prospective randomized controlled trials (RCTs) which compared the ω-3 polyunsaturated fatty acids supplemented with the standard PN on outcomes of adult patients with sepsis from January 1996 to June 2013.The methodological quality of the included studies was evaluated,and the Cochrane Collaboration RevMan 5.0 was used for data analysis.Results A total of 12 studies enrolling 721 patients were included.Significant reduction in 28-day mortality [relative risk (RR) 0.77,95％ confidence interval (95％CI) 0.59 to 0.99,P=0.04],short intensive care unit [ICU,weighted mean difference (WMD)=-3.10,95％CI-5.98 to-0.21,P=0.04] and hospital length of stay (WMD =-3.12,95％CI-4.65 to-1.60,P＜0.000 1) were observed in patients receiving ω-3 polyunsaturated fatty acids supplemented PN.There was no differences in days of mechanical ventilation between patients with or without adding ω-3 polyunsaturated fatty acids in PN (WMD =1.33,95％ CI-5.09 to 7.75,P=0.69).Conclusions Meta-analysis results demonstrated that PN supplemented with ω-3 polyunsaturated fatty acids was beneficial in improving the outcomes of patients with sepsis.However,this conclusion must be interpreted with caution due to the low quality of the enrolled trials.

Objective We tried to translate the English version of the Falls Efficacy Scale,Swedish version [FES (S)] into Chinese and test the reliability and validity of the Chinese version of FES (S) in patients after stroke.Methods Totally 145 patients after stroke were recruited and were investigated by the Chinese version of FES (S).The result underwent analysis of reliability and validity.Results The internal consistency coefficient of the Chinese version of FES (S) were 0.896.The Cronbach's α coefficient of two dimensions,which were Activity of Daily Living Ability and Instrumental Activity of Daily Living,were 0.863 and 0.872.The split half coefficient was 0.653,test-retest reliability was 0.743.The content validity index(CVI)was calculated according to related formulas,which was 0.896～1.000,the average CVI of all items was 0.925,CVI of each item was above 0.8.Factor analysis got two factors,which explained 40.852％ of the total variance.Except for item 5,other items showed factor loading ＞0.4 for its common factor.Conclusions The Chinese version of FES (S) has been proved to be reliable and valid.It can be used as a valid tool for assessing stroke patients with risk for falls and self-confidence.

ABSTRACT:Objective To investigate the interaction of polymorphisms of TNF-αgene promoter-308G/A and PPAR-γ2 gene-C34G with acute pancreatitis (AP)and its severity degree.Methods Totally 150 mild acute pancreatitis(MAP),150 moderately severe acute pancreatitis(MSAP)and 150 severe acute pancreatitis(SAP)cases were selected for this study,and 450 healthy persons as control group.The genetic polymorphisms of TNF-αgene promoter-308G/A and PPAR-γ2 gene-C34G were analyzed by the technique of PCR in peripheral blood leukocytes of above-mentioned cases and the results were verified by direct DNA sequencing method.Results The frequencies of -308G/A(GA),-308G/A(AA),-C34G(CG)and-C34G(GG)were 24.00%,26.67%,24.00% and 26.00% in MAP group,34.67%,36.67%,34.00% and 36.67% in MSAP group,42.00%,46.00%,43.33% and 46.00% in SAP group,and 14.44%,14.22%,12.89% and 14.67% in control group,respectively.Statistical tests showed significant difference in the frequencies among each group (all P<0 .0 1 ).The risk of AP significantly increased in subjects with-308G/A(GA),genotype (ORMAP=2.677 6,ORMSAP=6.625 0,ORSAP=21.514 7),in those with-308G/A(AA)genotype (ORMAP=2.570 0,ORMSAP=6.401 8,ORSAP=18.903 4),in those with-C34G(CG) genotype (ORMAP=2.668 4,ORMSAP=6.776 9,ORSAP=22.207 2),and in those with-C34G(GG)genotype (ORMAP=2.633 8,ORMSAP=6.472 5,ORSAP=21.570 2).Combined analysis of the polymorphisms showed that percentage of-308G/A(AA)/-C34G(GG)in MAP,MSAP,SAP and control groups was 7.33%,13.33%,20.67% and 2.00%,respectively,and statistical tests showed significant difference in the frequency among each group (all P<0.01).The people who carried-308G/A(AA)/-C34G(GG)had a high risk of AP (ORMAP=7.284 2,ORMSAP=41.296 1,ORSAP=363.973 6),and statistical analysis suggested a positive interaction between-308G/A(AA)and-C34G(GG)in increasing the risk of AP (γ2MAP=2.114 2,γ4MAP=2.080 0,γ2MSAP=2.108 7,γ4MSAP=2.050 6,γ2SAP=2.138 8,γ4SAP=2.000 1).Likewise,there were also positive interactions in the pathogenesis of AP between-308G/A(GA)and-C34G(GG),-308G/A(GA)and-C34G(CG),-308G/A(AA)and-C34G(CG)(All γ>1). Conclusion These carriers of-308G/A(GA),-308G/A(AA),-C34G(CG)and-C34G(GG)genotypes may have a high risk of developing AP,and significant interactions between genetic polymorphisms of-308G/A and-C34G add the risk of the occurrence and development of AP.