Objective To translate the English version of Control Attitudes Scale-Revised (CAS-R) into Chinese and to test the reliability and validity of the Chinese version of CAS-R in patients with heart disease.Methods Totally 125 patients with heart disease were recruited and were investigated by the Chinese version of CAS-R.Results The internal consistency coefficient of the Chinese version of CAS-R ranged from 0.735 to 0.862.Factor analysis got three factors,which explained 54.128％ of the total variance.Using BDI as an external criterion,the correlation coefficients of the CAS-R and BDI scores were-0.59.Pearson coefficient with three dimensions of BDI were-0.48、-0.51 and 0.42.Conclusions The Chinese version of CAS-R has been proved to be reliable and valid.It can be used as a valid tool for the measurement of perceived control in patients with heart disease.

In teaching of intra-arterial thrombolysis of ischemic stroke, introducing the method of problem-based learning ( PBL ) to improve the students' learning initiative and stimulate the students' interest in search of knowledge, and guide students to form scientific clinical thinking. Through further participation in surgery, promoting the combination of theory and practice. PBL can help to improve students' ability to analyze and solve problems related to intra-arterial thrombolysis of ischemic stroke.

Objective To explore the significance of ISO15189 in the cervical cancer and breast cancer screening.Methods Collected in 2009 and 2010 by the clinical laboratory in Hefei Adicon cervical cytology screening women ensure the submission of Anhui Funan Women BUPA 10050,28436 patients as research subjects,analysis of process management in accordance with ISO15189 Specifications in the detection of smears before and after implementation before,during and after the result of differences in the process of formation of. Results The quality of cervical smears and the positive rate had greatly improved, dramatically improve customer satisfaction. Conclusion ISO15189 implementation of process management standard “two cancer ” screening of cervical cytology screening provided quality assurance.

Objective To explore the failure factors of the immunization of hepatitis B vaccine,in order to provide evidence for adult hepatitis B vaccine immunization.Methods Random seleced the targets by examinations from susceptible population whose age were ≥18 years old,that immunized with 10?g Recombinant hepatitis B vaccine(YDV)three doses followng schedule of 0、1、6 months,then condcted a case-control study to the failure.Results Failure rate of adult administered with 10?g HepB immunity was 12.99%.Among the failures,smoking rate,obesity rate,family history hepatitis B virus infection rate and micro virus infection rate were higher than the control group.There was obvious statistic difference.Conclusion The adult failure factor of hepatitis B vaccine immunity was associated with smoking,obesity,hepatitis B viras infection,family history and micro virus infection.

The subject of clinical medicine contains abundant humanism educational material.When students start the clinic learning and contact with the patients for the first time,clinical teacher should endeavor to cultivate medical students' humanistic spirit in clinical practice.The paper discussed how to merge humanistic spirit into medical clinic teaching by illustrating the gynecology and obstetrics teaching.

Objective To evaluate the clinical efficacy of laparoscopic surgery in the treatment of endometriosis. Methods Clinical data of 102 patients with endometriosis treated by laparoscopic surgery were retrospectively analyzed. Results There were no conversions to open surgery or complications in all the patients. The operation time was 80 5 min ? 28 3 min, the intraoperative blood loss was 25 2 ml ? 23 2 ml and the postoperative hospital stay 3 0 d ? 1 2 d. Follow-up for 6 months ～ 66 months in 83 patients showed 21 cases of recurrence. Normal pregnancy was seen in 23 patients among 53 patients with endometriosis associated with infertility. Conclusions Laparoscopic surgery is effective for endometriosis, but the danger of recurrence should not be overlooked.

Objective:To investigate the expression change of voltage-gated calcium channels(VGCC) in the facial nucleus motoneurons of adult rats after facial nerve injury.Methods:The facial motor nucleus was localized by retrograde labeling with a fluorescent dye,Dil,and identified by Nissl staining.The facial nerve injury model was established by amputation of the main trunk of left facial nerve.Exposure of the right facial nerve without nerve transection was used as the control.Rats were sacrificed at 3,7,14 and 28 days after injury respectively(n =10),the brainstem containing facial nucleus were collected,the expression of P/Q,N,L,R-type calcium channel α1A,α1B,α1C and α1E subunits was examined by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR).Results:Immunohistochemistry results show that whereas α1A,α1E subunits levels did not vary compared with control group 3,7,14,28 days after injury(P ＞0.05),α1B and α1C subunits immunoreactivity decreased in the motoneurons after injury,a sharp decrease was detected at 14 days after injury(P ＜ 0.01),thereafter returned to the control level at 28 days after axotomy(P ＞0.05).The expression of α1B and α1C mRNA was down-regulated,especially 14 days after the injury(P ＜0.01),and then recovered to normal level at 28 days (P ＞ 0.05).In addition,there was no significant difference of α1A and α1E subunits and their correspoding mRNA between operated group and control group at all time points(P ＞ 0.05).Conclusion:VGCC is involved in facial nerve injury and down-regulation of N-type and L-type calcium channels may be one of the role.

ObjectivesTo study the mechanism of brain damage caused byStaphylococcus epidermidis (SE) in mice. Methods A total of 80 neonatal mice of postnatal day 1 (PND1) were divided into SE group (48 mice), normal saline (NS) group (16 mice) and control group (16 mice). Mice in SE group were intravenously injected with 50 μl SE (108/ml). Mice in NS group were given 50 μl NS. Mice in control group were not intervened. At different time points after SE injection (6 h, 24 h, 72 h, 5 d, 7 d), the CFU of brain, blood, and spleen were calculated. Serial sections of parafifn-embedded brain tissue were used for detection of ionized calcium-binding adaptor moleculor1 (Iba-1) by immunohistochemical staining. The positive cells were calculated. ELISA was used to measure the levels of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-5 (IL-5), interleukin-6 (IL-6) of brain at 6 h and 24 h after SE injection.Results There was no SE in brain in different time points. The CFU was at the highest level at 6 h and then decreased after 24 h in blood and spleen. The Iba-1 positive cells in SE group were signiifcantly increased compared to NS group and control group at 24 h and 72 h (P<0.05). There was no difference of Iba-1 positive cells be-tween 24 h and 72 h after SE injection (P>0.05). The levels of TNF-α, IL-1β, IL-5, and IL-6 were signiifcantly higher in SE group than those in NS and control at 6 h and 24 h (P<0.05). The levels of TNF-α, IL-1β, IL-5, and IL-6 were signiifcantly lower in SE group at 24 h than those in SE group at 6 h (P<0.01).Conclusions It is suggested that cytokines produced by microglias may be the mediators of SE-caused brain damage.

Objectives To study the influence of Staphylococcus epidermidis (SE) on the neonatal mice of different ages. Methods A total of 60 neonatal mice including postnatal day 1(PND1) and postnatal day 3(PND3) were divided into SE group, normal saline (NS) group and control group, with 20 mice each. Mice in SE group were intravenously injected with 50μl SE (108/ml). Mice in NS group were given 50μl NS and mice in control group was not intervened. On postnatal day 14, the brain, liver and spleen obtained from mice were weighted. Serial sections of paraffin-embedded brain tissue were used for the detec-tion of microtubule associated protein-2 (MAP-2) and myelin basic protein (MBP) by immumohistochemical staining, and then the areas and volumes of grey and white matter were calculated. Result The mortality of PND1 mice in SE and NS group was 60.0%and 40.0%, respectively, and there was no difference between two groups (P>0.05). The mortality of PND3 mice in SE and NS group was 10.0%and 0.0%, respectively, and there was no difference between two groups (P>0.05). There were no dif-ferences in body weight, body weight gain, spleen and liver weights and organ coefficient between PND1 and PND3 mice (P>0.05). In PND1 mice, the areas and volumes of grey and white matter were significantly smaller in SE group than those in NS group (P<0.05). However, in PND3 mice, there was no differences in areas and volumes of grey and white matter between SE and NS group (P>0.05). Conclusions SE infection can result in brain injury in PND1 mice, but has no effect on brain tissues of PND3 mice.

Objective To develop a new visual feedback postural adjusting ability assessing system, and to study its application value. Methods Pressure sensor was used to determine the changes of the body weight center by tracing the pressure variation of the soles and heels, then eight target points in different directions were added into the software interface to enable the subject move his body weight center to the target point intentionally, finally the system was evaluated by loading on back.Results Program design was performed for the testing system. It's proved that posture adjusting ability might decrease with 20% load on back or the body leaning backwards. Conclusion The newly developed visual feedback postural adjusting ability testing system can be used to research standing balance function.