OBJECTIVE:To determine the plasma mycophenolic acid concentration by HPLC, and study the multidoses pharmacokinetics character of mycophenolic acid in Chinese kidney transplantation patients. METHODS: The samples were precipitated with acetonitrile before injection. Diamonsil C18 column was used. The mobile phase consisted of acetonitrile-10 mmol?L-1 KH2PO4 (5∶6) at a flow rate of 1.1 mL?min-1. The detection wavelength was 254 nm and the column temperature was 40 ℃. This method was used to determine the multidoses pharmacokinetics in 12 kidney transplantation patients. RESULTS: MPA was well-separated from internal standard in chromatography, and endogenous foreign substance in plasma had no interference on the determination. The liner range for MPA was 0.38～59.00 ?g?mL-1,and the lowest detectable concentration of MPA was 0.38 ?g?mL-1. The recovery rate stood at 89.32%～97.63%; Both intra-day and inter-day RSD were less than 8.00%. Significant individual difference was noted among the patients treated with MMF in pharmacokinetic results, which was in line with the literature. CONCLUSION: This method is accurate and simple and applicable for the pharmacokinetics study of mycophenolic acid.

OBJECTIVE:To explore the feasibility of using international index of rational drug use for the intervention and evaluation of rational use of antimicrobial agents during perioperative period in patients undergoing thyreoidectiomy.METHODS:The patients in the common surgical department were divided into intervention group and non-intervention group.An intervention control study was performed before intervention(from Mar.to Aug.in 2007)vs.after intervention(from Mar.to Aug.in 2008)on the synchronously discharged thyreoidectomy patients with the international index of rational drug use as references.RESULTS:After prophylactic use of antibiotics in the intervention group,the average antimicrobial application time was decreased from 6.83 days to 2.50 days,the average hospital stay of patients was decreased from 8.24 days to 7.15 days,the average hospitalization fee decreased from 1 895.99 yuan to 1 375.26 yuan,the cost of antibacterials decreased from 852.11 yuan to 538.53 yuan,and the proportion of the cost of antibacterials in the total hospitalization cost reduced slightly.However,all the indexes in the non-intervention group showed little change.CONCLUSION:It is effective and feasible to adopt the international index of rational drug use in the intervention and evaluation of rational use of antimicrobial agents during perioperative period.

Objective:To research the rule of melatonin nasal absorption.Methods: The stability of melatonin in rat nasal wash was studied, after that the in situ rat nasal recirculation methods were used as experimental animal model to study the rule of melatonin nasal absorption.Results:Melatonin was stable in rat nasal wash, its absorption in nasal cavity accorded with first order kinetic equation. The absorption rate constants did not vary with melatonin concentrations.Conclusion:The mechanism of melatonin nasal absorption is passive diffusion, the average absorption rate constant is 1.054?10 -2 min -1 .

OBJECTIVE:To systematically review the efficacy and safety of Moxifloxacin(MFX)in the treatment of acute ex-acerbation of chronic bronchitis (AECB),and provide evidence-based reference for Tational use of MFX. METHODS:Retrieved from PubMed,EMBase,Cochrane Library,Medline,CBM,CJFD,VIP and Wanfang Database,the relevant conference proceed-ings and grey literature were also hand-searched,randomized controlled trials(RCT)about MFX(test group)versus other antibiot-ics (control group) in the treatment of AECB were collected. Meta-analysis was performed by using Rev Man 5.2 software with ITT analysis and PP analysis after literature selection,data extraction and quality evaluation. RESULTS:Totally 14 RCTs were in-cluded,involving 6 058 patients. Results of PP analysis showed,the clinical effective rate in test group was similar to that of con-trol group [RR=1.02,95%CI(1.00,1.04),P=0.06],while bacteria clearance rate was significantly higher than control group [RR=1.07,95%CI(1.04,1.11),P<0.001]. Results of ITT analysis showed,the clinical effective rate in test group was significantly high-er than control group [RR=1.03,95%CI(1.00,1.06),P=0.03],while there was no significant difference in the bacteria clearance rate [RR=1.02,95%CI(0.92,1.12),P=0.73] and the incidence of adverse reactions [RR=0.97,95%CI(0.87,1.08),P=0.52] be-tween 2 groups. CONCLUSIONS:The efficacy of MFX is not inferior to other antibiotics in the treatment of AECB,safety is simi-lar to other antibiotics.