Livin is the inhibitor of apoptosis proteins (IAPs), a new member, in most malignant tissues of high expression, and its main function is to inhibit cell apoptosis. Apoptosis and tumor cells is closely related to the occurrence and development, more on which the survival of tumor angiogenesis. Vascular endothelial growth factor (VEGF) is one of the strongest of vascular growth factor, and almost all human tumors and tumor cell lines have over-expression of it. Livin and VEGF protein expression in tumor was summarized.

Objective To find out the factors which influence the establishment of collateral circulation as well as its clinical characteristics by analyzing the sufferers' medical records.Methods Review and collect the records of the first coronary angiography,and we can find the total occlusion of one coronary after coronary angiography.There are 52 cases in which people who suffered the establishment of collateral circulation stay in hospital for treatment.Here,we take 52 cases in which people didn't suffer into comparison and analyze these clinical records.Results Use the Logistic regression method to analyze the relationship between general clinical data,past history,habits,current medication and the establishment of collateral circulation,which relates to the diabetes mellitus anamnesis,exercise and drinking habits,with the regression coefficient is 0.408,0.483,0.197(P

Objective To explore 24-hour ambulatory blood pressure of patients with cervical spinal cord injury. Methods 42 patients with cervical spinal cord injury and 45 patients with thoracic-lumbar spinal cord injury were included. Their 24-hour ambulatory blood pressures were monitored. Their day systolic blood pressure (dSBP) and day diastolic blood pressure (dDBP) when seating and standing, night SBP and DBP, and SBP descending rate at night were compared. Results There were significant differences in dSBP and dDBP when standing, and SBP descending rate at night between 2 groups (P<0.01). There was significant difference in dSBP between seating and standing position in patients with cervical spinal cord injury (P<0.01). Conclusion There is orthostatic hypotension in cervical spinal cord injury patients and their SBP descending rate at night is upside down.

Objective To discuss the early clinical management of the pa tients with traumatic hemipelvectomy. Methods A case was reported and literature reviewed. Results The patient was a 4-year-old boy who incurred a left groin and proximal thigh crush with nearly complete transection of this extremity and arrived at our hospital 7 hours after injury. After the vital signs were stabili zed with reasonable wound care and 2 hours of fluid resuscitation, the patient r eceived a thorough debridement and the hip disarticulation and fixation of pubic symphysis. The postoperative course was relatively smooth. While the successful treatments of this patient corresponded to the experiences in the literature, s pecial considerations regarding this severe injury were discussed. Conclusion Im mediate prehospital hemostasis by local pressure, rapid transport, aggressive in itial resuscitation, early recognition of the severity and prompt decision to co mplete the amputation, and attention to details in the rehabilitation phase are necessary for successful management of these patients.

OBJECTIVE:To establish a method for determination the genotoxicity impurities (methyl methanesulfonate,ethyl methanesulfonate and isopropyl methanesulfonate) in mesylate nafamostat raw materia. METHODS:GC-MS was conducted,and the genotoxicity impurities were extracted by dichloromethane. The column was DB-5 capillary column by programmed tempera-ture,the inlet temperature was 240 ℃,column flow was 3.0 ml/min,purge flow was 6.0 ml/min,sample mode splitless injection, carrier gas was high purity helium,detector is a mass spectrometer detector,ion source temperature was 230 ℃,the interface tem-perature was 230 ℃,the delay time of solvent was 2.5 min,ionization mode was electron impact,detector voltage was respect to the tuning results,scanning(detection)method was selective ion monitoring,electron energy was 70 eV,and the injection volume was 1.0μl. RESULTS:The separation degree of 3 impurities were greater than 2.0;the linear range of 3 impurities were 0.10-20μg/ml (r≥0.999 5);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 97.7%-104.8%(RSD=2.8%, n=9),102.5%-110.7%(RSD=2.6%,n=9)and 103.0%-107.6%(RSD=1.6%,n=9). CONCLUSIONS:The method is simple, accurate,sensitive and rapid,and can be used for the genotoxicity impurities in mesylate nafamostat raw materia.

Objective To investigate the effect of ulinastatin on hippocampal neuron and matrix metalloproteinase-9 ( MMP-9 ) expression after ischemia-reperfusion injury rats, to reveal the mechanism of protection on hippocampus of ulinastatin.Methods The focal ischemia reperfusion model was made by thread embolism of middle cerebra1 artery, and ulinastatin was used.The animals were killed at 24 h after reperfusion was started.The sections of the brains were processed with Nissl staining and by immunohistochemistry with antibody of MMP-9.Results Corrected optical density (COD) of MMP-9 expression was significantly decreased after administration of ulinastatin.Also ulinastatin can obviously protect the structure of hippocampal neuron.Conclusion Ulinastatin could protect pyramidal cells in CA1 region from damage of ischemia-reperfusion injury and increase the number of normal neurons.Inhibition on MMP-9 expression was involved in the mechanism.

Objective To find out the factors affecting the outcome of severe acute respiratory distress syndrome (ARDS) patients treated with extracorporeal membrane oxygenation (ECMO).Methods From November 2008 to September 2010,patients with severe acute respiratory distress syndrome (ARDS) required extracorporeal membrane oxygenation (ECMO) after failure of conventional therapy were retrospectively studied.Patients treated by veno-arterial ECMO,treated by mechanical ventilation for more than l0 days were excluded.All patients were divided into survival group and non-survival group,according to the 28 day survival situation after ECMO treatment.We compared the clinical data of the two groups when transferred to Intensive Care Unit (ICU),before and after ECMO treatment.We used Independent-samples t test and Chi-square tests to find out the factors affecting the results of ECMO treatment in the patients with severe ARDS.Results A total of twenty-five patients were enrolled in the study,of whom 15 patients survived,10 patients died.The results showed that the average age of the survival group is lower than that of non-survival group (49.8 ± 10.5) vs.(59.9 ± 11.5),P =0.044,and the proportion of patients with severe ARDS caused by Influenza A (H1 N1) in the survival group was higher than that of the non-survival group (x2 =4.453,P =0.048).In addition,the duration of mechanical ventilation before ECMO treatment in the survival group was shorter than that in the non-survival group (57.8 ± 8.7) hours vs.(68.3 ±13.7) hours,P =0.013.Conclusions The age,pre-ECMO ventilation duration and the cause of ARDS are the important factors influencing the efficacy of ECMO treatment in the patients with severe ARDS.

Modern Chinese Orthopaedic traumatology has stemmed from the orthopaed ics of Traditional Chinese Medicine(TMC)and western orthopaedics.On the one hand,with a long history and its unique system of theory and treatment,TMC orthopaedics h as made a great con-tribution to the development of modern Chinese orthopaedic traumatology.On the other hand,the introduction and development of western medicine have dramatically pro moted the development of modern Chinese orthopaedic traumatology.Modern Chinese surgeons of orthopaedic trauma have accomplished numerous successful f irst cases in the world since 1950s an d have thus expanded their professional influence.At pr esent,the Chinese orthopaedic trau ma community has grown much stronger and established its ow n academic associations and journals.More and more de-partments of orthopaedic traumatology have been successively establis hed as an independent en-tity in large and middle-sized hospi tals in China.So far,the Chinese orthopaedic trauma com-munity,on the whole,has reached the international level,but there are q uite a lot to be improved in the aspect of treatment.To end the backwardness,in the future Chinese orthopaedic trauma community should make great efforts in education of orthopedists on comp rehensive knowledge,establishment of formal profession al training system,national standardization of orthopaedic procedures in clinical diagnosis an d treatment,active membership of re lative international aca-demic associations and increasing p rofessional exchanges with international counterparts,as well as in research and development of operative techniques and fixators.All these will be the main strategies and developmental trends in the future for Chinese orthopaed ic traumatology. [

Objective To investigate the effect of dexmedetomidine hydrochloride on hemodynamics,immune function and inflammatory factors in patients with gynecological laparoscopic operation.Methods According to the random comparison table,86 elderly patients with gynecological laparoscopic operation were divided into observation group(43cases) and control group(43cases).The observation group received conventional general anesthesia,and intravenously pumped with dexmedetomidine hydrochloride.The the control group received conventional general anesthesia,and intravenously pumped with normal saline.The changes of hemodynamics,immune function and inflammatory factors were observed and compared in different periods in the two groups.Results Compared with before induction,the level of HR increased significantly at intubation(t=-3.257,-5.019,all P<0.05),the level of HR decreased significantly at 15 min and 30 min after pneumoperitoneum in the two groups(15 min after pneumoperitoneum:t=2.111,-5.019;15 min after pneumoperitoneum:t=3.078,3.926,all P<0.05),but the changes of the observation group variation were significant smaller(at intubation:t=-2.078;15 min after pneumoperitoneum:t=2.105;30 min after pneumoperitoneum:t=1.993,all P<0.05),the level of MAP had no significant difference at intubation in the observation group(t=1.893,P>0.05),the level of MAP increased significantly at intubation in control group(t=-2.122,P<0.05),and there was significant difference at intubation in the two groups(t=-3.477,P<0.05).Compared with before induction,the levels of HR and MAP recovered in the two groups,there were no statistically significant difference at 24 h after operation(HR:t=0.474,0.233;MAP:t=0.853,-0.273,all P>0.05);Compared with before induction,the levels of CRP and IL-6 increased significantly at after operation and 24 h after operation in the two groups(CRP:t=-9.974,-16.872;IL-6:t=-7.284,-11.449,all P<0.05),and the levels of CRP and IL-6 increased more significantly in the control group(CRP:t=-7.509;IL-6:t=-4.744,all P<0.05);Compared with before induction,the levels of CD+3,CD+4,CD+4/CD+8 and NK had no statistically significant differences at after operation and 24 h after operation in the observation group(t=0.154,1.204,1.546,-0.289,all P>0.05),the levels of CD+3,CD+4,CD+4/CD+8 and NK decreased significantly after operation in the control group(t=11.514,10.317,9.180,6.815,all P<0.05),there were statistically significant differences after operation in the two groups(t=10.232,10.298,7.728,4.900,all P<0.05),and the level of NK decreased significantly at 24h after operation in the control group(t=4.362,P<0.05).Conclusion Dexmedetomidine hydrochloride can keep hemodynamics stability,reduce immune function damage and inflammation.

Objective: To study the effects of different restorati ve materials on the health of periodontal tissue. Methods: A total of 40 posterior teeth were divided into four groups with 10 in each. Sil ver amalgam, glass ionomer cements, GlasIonomer Cement FX and light curing Mic roglass composite resin were used to restore Class Ⅱ cavity in each tooth of th e 4 groups respectively . 6 months after restoration gingival cervical fluid (GC F) was collected , GCF aspartate aminotransferase (GCF AST) level was tested a nd plaque index was assessed for each case. Results: The s ilver amalgam and light curing Microglass composite resin groups presented less amount of GCF ( P