Objective: To study the effects of different restorati ve materials on the health of periodontal tissue. Methods: A total of 40 posterior teeth were divided into four groups with 10 in each. Sil ver amalgam, glass ionomer cements, GlasIonomer Cement FX and light curing Mic roglass composite resin were used to restore Class Ⅱ cavity in each tooth of th e 4 groups respectively . 6 months after restoration gingival cervical fluid (GC F) was collected , GCF aspartate aminotransferase (GCF AST) level was tested a nd plaque index was assessed for each case. Results: The s ilver amalgam and light curing Microglass composite resin groups presented less amount of GCF ( P

Objective To investigate the serum level of NT-pro-BNP in patients with acute ischemic stroke and to determine whether NT-pro-BNP levels were associated with the death within 15 days of stroke onset. Methods Two hundard twenty-six consecutive patients with acute ischemic stroke within 48 hours of onset were enrolled in this study. We measured plasma NT-pro-BNP within 72 h and recorded the NIHSS score on admission. Patients were divided into two groups: the deceased group, who died within 15 days, and the survival group. The factors associated with the death within 15 d of stroke onset were investigated by using multivariate logistic regression analysis. Results Twenty-four (10.6%) patients died with 15 days of stroke onset. The incidence of atrial fibrillation, cardioembolism and large infarc?tion, the mean ± SD of NIHSS score, age, glucose level and creatinine were significantly higher in the deceased group than in the survival group (P<0.001). On the other hand, the mean ± SD of LVEF, albumin, LDL-C, and total-cholester?ol were significantly lower in the deceased group than in the survival group(P<0.05 ). The median of the plasma NT-pro-BNP level was significantly higher in the deceased group than in the survival group (2598.5 vs. 190.4 pg/mL, P<0.001). The optimal cut-off level, sensitivity, specificity and ROC area of NT-pro-BNP levels to distinguish the de?ceased group from the survival group were 955.2 pg/mL, 83.3%and 82.2%, 0.906, respectively. Binary logistic regression analysis demonstrated that NIHSS score of ≥13 (OR=56.18, 95% CI=9.06 to 348.40, P =0.000) , plasma Lg NT-Pro-BNP level (OR=38.79, 95%CI=6.52 to 230.95, P=0.000) , and the size of infarction (OR=8.73, 95%CI=1.11~68.88, P=0.040) were independent factors associated with the death within acute phase of stroke. Conclusions The plas?ma NT-pro-BNP level can predict the death of stroke patients within 15 days of stroke onset.

Objective To observe the ultrastructural change of intestinal mucosa in mice infected with Blastocystis hominis, and to study the pathogenic mechanism of B.hominis infection. Methods 20 Kunming mice were randomly divided into 4 groups: group A treated with immunosuppressant (dexamethasone), group B without immunosuppressant, group C as normal control and group D as immunosuppressant control. Groups A and B were then orally infected with 204 cysts of B. hominis. Groups C and D were treated as control by infusing same volume of Locke′s solution. Six days after inoculation, mice in each group were killed and mucosa of ileocecum was observed by transmission electron microscope (TEM) and scanning electron microscope (SEM). Results Under SEM, B. hominis located in enteric cavity and on the surface of ileocecum mucosa. Individual parasites also invaded into mucosa and its fold. Partial destruction of microvilli on the mucosa was observed. TEM observation indicated a reduction of microvilli on the surface of absorptive cells. Mitochondrial edema, rough endoplasmic reticulum dilatation and degranulation were found on absorptive cells and goblet cells. Lymphocyte infiltration and eosinophilia were found in intercellular stroma. Pathological changes in group A were more serious than that of group B. No abnormal change on the mucosal ultrastructure was found in groups C and D. Conclusions B. hominis infection causes significant ultrastructural lesion on the ileocecal mucosa in mice. Immune status of the mice can affect the degree of the lesion due to infection.

Objective To evaluated the role of wt-P53 protein in telomerase regulation in keloid fibroblasts(KFBs). Methods The fibroblasts were derived from human keloid tissue which was proved by pathological diagnosis. KFBs were divided into 2 groups, the transfection group and the untransfection group. wt-p53 gene was transfected into the fibroblasts by adenovirus vectors in the transfection group. The KFBs untransfected with wt-p53 gene served as control (untransfection group). After 48 hours of transfection, the expression of wt-P53 protein was analyzed by both Western blotting and immunofluorescence method, respectively. The telomerase activity was evaluated by TRAP-ELISA after 1-7 days of transfection.Results All the KFBs from 2 groups expressed wt-P53 protein. But the expression level of wt-P53 protein in the transfection group was significantly higher than that in the untransfection group. At the same time of high expression of wt-P53 protein, the telomerase activity of KFBs in transfection group was significantly lower than that in the untransfection group( P＜0.05). Conclusion High level expression of wt-P53 protein can transiently inhibit the telomerase activity of KFBs.

E.coli single-stranded DNA-binding protein (SSB) plays an important role in replication, recombination and repair of DNA and is thus crucial for the survival of the bacteria.We described a high expression and efficient purification scheme and kinetic assay of interaction with its substrate, single-stranded DNA (ssDNA). A ssb gene (537 bp) for encoding SSB was obtained by PCR amplification from E.coli K-12 genome. The expression vector of the fusion protein SSB was constructed by attaching ssb gene to pQE30. SSB fusion protein was expressed in M15 E.coli strain induced by IPTG. SDS-PAGE analysis revealed that the expected protein with a molecular weight 20.6kDa was soluble and amounted to about 30% of the total bacterial protein. SSB protein was purified by immobilized metal (Ni2+) chelation affinity chromatography and the purity was about 90%. The resulting SSB protein was a correctly folded tetramer analyzed by gel filtration. It could bind ssDNA with equilibrium dissociation constant (KD) of 4.79×10-7 mol/L as determined by surface plasmon resonance.

Objective:To induce the specific cytotoxicity of T lymphocytes to the breast cancer cells overexpressing HER 2 by bispecific monoclonal antibodies anti HER 2?anti CD3 and to stimulate natural immunoreactions. Methods:Using SK BR 3 cell line overexpressing HER 2 as target cells, MCF 7 cell line as control cells, and T lymphocytes as effector cells, the biological function of BsAb anti HER 2?anti CD3 was studied, including growth inhibition of target cells, proliferation of effect cells, and cytotoxicity of lymphocytes mediated to SK BR 3. The measuring methods included MTT(measurement of tetrazplium), 3H thymidine incorporation assay system.Results:BsAb anti HER 2?anti CD3 had the function of MAbHER 2 and MAbCD3, which inhibited growth of SK BR 3 and accelerated proliferation of T lymphocytes from normal donors. BsAb anti HER 2?anti CD3 mediated more significant cytotoxicity of T lymphocytes to SK BR 3 than MAb HER 2.The best ratio of effector cells to target cells was 20∶1. Conclusion:BsAb anti HER 2?anti CD3 bound to the breast cancer cells overexpressing HER 2 and T lymphocytes and mediated significant cytotoxicity of T lymphocytes to the breast cancer cells overexpressing HER 2, indicating BsAb anti HER 2?anti CD3 had significant antitumor function.

Objective To study wt-p53 gene's influence on the proliferation of keloid fibroblasts in vitro. Methods wt-p53 gene was transfected into keloid fibroblasts by adenovirus vector. wt-p53 mRNA was analyzed by RT-PCR; wt-p53 protein was evaluated by indirectiy immunofluorescence; The ability of proliferation of keloid fibroblasts was analyzed by cell growing curves; The cell cycle of KFB was checked by FCAS. Results The expression of wt-p53 mRNA and protein was obviously higher in the fibroblasts of the experimental group than those of control group; the rate of G_0~G_1 in cell cycle was higher in the fibroblasts of the experimental group than those of control group; at the same time, the rate of G_2~M was lower in fibroblasts of the experimental group than those of control group (P

Objective To survey on the prevalence of schistosomiasis among floating population in Zhejiang province. Methods A survey on prevalence of schistosomiasis among floating population was conducted from September to November 2008, and the stratified cluster sampling method was adopted in the survey. Totally 129 villages of 19 counties or districts were selected as survey sites, and 100 samples of migrants aged 6 to 65 from schistosomiasis-endemic areas were taken in each selected village. All selected individuals were surveyed by questionnaire and underwent serum indirect hemagglutination (IHA) test. For individuals with positive serum IHA testing the fecal examination was carried out to detect the eggs by nylon sedimentation method. SPSS 13.0 software was used for data processing. Results The number of migrants in survey sites was 3 357 420, among whom 303 219 were from schistosomiasis-endemic areas (9.03%).The positive rate in serum IHA test was 2.06% (286/13 898), 276 IHA-positives individuals received fecal examination, and 7 cases were positive (2.52%). Based on above data it was estimated that there would be potentially about 33 500 serum IHA-positive cases and 845 egg-positive cases among floating population in Zhejiang province. Conclusion The risk of schistosomiasis transmission still exists in Zhejiang province due to the infected migrants from endemic areas, and a surveillance system and quick response are required for prevention of re-emergence of the disease.

Objective To summarize the progress in dermatological researches in the past 5 years achieved domestically and abroad,and venture to propose the developmental orientation and problems to be emphasized in dermatology in the next 5 years. Methods The achievements and progresses gained in dermatology in the recent 5 years were retrieved by employing the well-know informatics technology. Results Remarkable achievements have been gained in dermatological researches during recent 5 years,especially those concerning psoriasis,connective tissue diseases,vitiligo and dermatomycosis. Two professional journals in dermatology were founded by military medical service in recent 5 years. Several awards for the achievements in dermatology were rewarded,such as,one each,the first-and second-class Army Science and Technology Progress Award,second-class Army Medial Achievements Award,second-class Chinese Science and Technology Award in medicine,provincial level second-class Science and Technology Progress Award,etc. Apart from those awards,researches on investigation and prevention of skin diseases during military training had made important breakthrough. Conclusions In the next 5 years,the points of focus in dermatological research should still be put on serving the basic army units,raising the level of prevention and treatment of skin diseases in the course of training,ensuring the psychological health of soldiers,supporting the important research items and promoting further the development of dermatology.

Analysis is made to the necessary of interdisciplinary,and point four sections should be concerned when begin to construct the interdisciplinary in general hospital which are short term profits and long term culture,personal development and team building,the passion of the young and the leadership of the old,the cooperation with the science and engineering and the communicate with the humanities.At last it also lists the steps and main points on how to begin the construction of interdisciplinary in general hospital.