Objective To investigate the effects of antioxidant on the gene expression of ?1-adrenergic receptors (AR) during endotoxic shock. Methods Forty male SD rats weighing 200-250 g were anesthetized with intraperitoneal (i.p.) pentobarbital. The animals kept spontaneous breathing. Femoral artery and vein were cannulated for BP monitoring and drug administration. The animals were randomly divided into 5 groups ( n = 8 each) : (1) control group; (2) LPS group received IPS 15 mg?kg-1 i.v. ; (3) LPS + propofol group received at 1h after LPS a bolus of propofol 10mg?kg-1 i.v. followed by continuous infusion of propofol at 10 mg?kg-1?h-1; (4) LPS + uric acid (UA) group received uric acid 200 mg i.p. 1 h after LPS and (5) LPS + melatonin (MLT) group received MLT 10 mg?kg-1 i.p. 1h after LPS. The animals were killed at 6 h after LPS. Total RNA was extracted from the heart, aorta, vein, lung, liver and kidney. ?1A-,?1B-,?1D- AR mRNA and ?-actin mRNA were measured using reverse transcription polymerase chain reaction (RT-PCR) .Results The expression of ?1A-AR and ?1B-AR mRNA in all the organs and the ?1D-AR mRNA expression in aorta, liver, lung and kidney were strongly down-regulated in LPS group. In group 3 (LPS + propofol) the expression of ?1A-AR mRNA in the lung and kidney, the ?1B-AR mRNA expression in all of the organs and ?1D-AR mRNA expression in aorta, liver, lung and kidney were significantly increased as compared with LPS group. There was no significant difference in ?1-AR mRNA expression between LPS group and LPS + MLT group. The expression of ?1A-AR mRNA in kidney, the ?1B-AR mRNA expression in all of the organs except the lung and the ?1D-AR mRNA expression in aorta, liver, lung and kidney were significantly higher in LPS + UA group than in LPS group. Conclusion Circulatory failure induced by endotoxic shock is related to the down-regulation of ?1-AR gene expression. The therapeutic effects of antioxidant on the endotoxic shock is partly due to up-regulation of ?1-AR gene expression.

Objective To investigate the effects of fruit potassium supplement on hypokalemia at in the perioperative period of percutanous coronary intervention(PCI)? Methods Ninety cronary herat disease patients with hypokalemia undergoing PCI during July to December 2011 involved the study? Since admission,the patients were administered with oranges and bananas each 500 g daily until the third day after PCI,on the basis of oral administration of potassium? Serum potassium was assessed in all patients at time points of the day at PCI,the first day and third day after PCI? Results The time range for the patients to take potassium supplement by eating fruits was 12-45 hours,averaged(28?84 ± 11?70)hours? The serum potassium levels on days 1 and 3 were significantly higher than that on the day for the admission(P < 0?05 for both)and even maintained at the normal level? Conclusion The oral complement of potassium by eating bananas and oranges can help patients to correct and prevent hypokalemia during the perioperative period of PCI?

OBJECTIVE To investigate extended-spectrum ?-lactamases(ESBLs) producing bacteria and daily(defined) doses (DDDs) of the third generation cephalosporins.METHODS The cosumpsion of the third generation cephalosporins in five(hospitals) from 2000 to 2002 and the incidence of ESBLs producing bacteria were(analyzed).RESULTS In these years,the total DDDs were 1.3064 million therapeutic days,0.3335 million in 2000;0.4312 million in 2001;(0.5417) million in 2002.The average ESBLs producing ratio was 33.67%,ie,29.64%,32.58% and(38.45%) in 2000,2001 and 2002.CONCLUSIONS The incidence of ESBLs producing bacteria and DDDs of the third generation cephalosporins are raised gradually. More faster raised DDDs would result in higher incidence of ESBLs producing bacteria.

[Objective] Discussion about feeding Liuwei dihuang pil to different ages of mental retardation intel igence-type mice, then test their intel igence level and mechanism. [Methods]The changes of ethology expressions are determined in healthy KunMing mice of five groups divided randomly:male mice (n=5), female mice(n=20). Group A:normal control group;Group B:the group with innate kidney deficiency and lead ingested. Group C:the group of lead poisoning, and the offspring mice are administered pil s. Group D:the group with innate kidney deficiency and lead ingested, and the adult mice are adminis-tered pil s. Group E:the group with innate kidney deficiency and lead ingested, and both the adult mice and offspring mice are administered pil s. According to the proportion of male and female mice, make them mate, remove the male mice. Before delivery, do threat means and give 0.1%Lead acetate trihydrate solution to Groups B,C,D,E.Groups D,E have tonifying the kidney. Give routine food to Groups A,B,C. Female mice wil labor in 19~22 days after mating, getting the offspring mice. The offspring mice in different periods of growth are carried out ethology determination and analyzed.[Results]Newborn rat kid-ney stand test shows rat offspring is not better than the success rate of kidney emancipated group, juvenile rat kidney tests show space group behavior test per-formance superior to that of kidney group, and long-term kidney group(offspring, mother on behalf of both rat kidney) or female offspring than only on be-half of kidney groups, multiple comparisons between groups(LSD-t statistical analysis) are statistical y significant, A group compared with B group, C group, D groups respectively(t=27.50,8.10,9.30,3.00,P<0.05), B group compared with C group, D group, E group(t=19.40,18.20,27.80, P al <0.05), C group and D group have difference statistical y significant(t=8.40, P<0.05), D group and E group difference is statistical y significant(t=9.60, P<0.05), young rats tightrope test, kidney group to some extent, is stil better than not Bushen group, multiple comparisons between groups find that:A group and D group, B group and C group, B group and E group, C group and D group, D group and E group have differences statistical y significant(t=22.01, 25.41,20.90,32.11,27.60, P<0.05). [Conclusion]Liuwei Dihuang Pil can improve the intel igence level of mice to a certain extent.

Objective To evaluate the applied value of CT guiding the liver cancer ablation with cluster electrode radiofrequency hyperthermia. Methods 105 cases with local lung cancer were ablated with cluster electrode radiofrequency hyperthermia by whole body CT-ELSCINT 2400ELECT. Results Success rate of 105 cases CT guiding was 98.3% and the total remission rate was 82.8%. Conclusion It is with CT that the location of percutaneous puncture can be optimally selected, and the point view and depth of the electrode can been detected anytime; also, the internal local focus can be monitored. So, the CT guiding is the optimal method to guide cluster electrode radiofrequency hyperthermia ablation for lung cancer.

Objective To study the difference between images of DR with or without equilibrated reconstruction. Methods 200 samples undergoing DR abdominal imaging from April 2003 are chosen orderly. Results All details of different tissue can be displayed in an image by adopting Equilibrated Reconstruction, while standard DR images should adjust the width and position of the window to get the same effect. Conclusion Equilibrated Reconstruction technology could improve image quality of different tissues without loss of details of other parts, and make DR more consummate and operation more convenient. It raise the ratio of displaying lesions.

Objectives To explore the effects of hemodialysis on oxidizing stress and plasma nitrogen monoxide (NO) levels in maintenance hemodialysis patients. Methods 67 maintenance hemodialysis patients (male 39, female 28, and mean age 58?13y) were enrolled in this study. Serum malondialdehyde (MDA) level was measured by colorimetry, and plasma NO level was determined by enzymatic method. The MDA and NO levels before and after hemodialysis were measured in 48 cases of the patients. Results After hemodialysis, the serum MDA levels significantly increased, and the plasma NO levels decreased. The patients with cardiovascular diseases had lower NO levels and higher MDA levels than the patients without ones before hemodialysis. The serum MDA levels of the patients using biological incompatible dialysis membrane increased after hemodialysis. Conclusion Hemodialysis treatment, especially using biological incompatible dialysis membrane increased oxidizing stress and decreased plasma NO levels in maintenance hemodialysis patients, and was significantly correlated with the pathogenesis of cardiovascular diseases.

BACKGROUND:Previous studies on posterior cervical single door and double door laminoplasty for repair of multilevel cervical myelopathy mainly focus onneurological function and clinical parameters and lack of certain comprehensiveness.
OBJECTIVE:To explore the effects of posterior cervical single door and double door laminoplasty for repair of multilevel cervical myelopathy.
METHODS:We selected 120 patients with multilevel cervical myelopathy and randomly divided into single door group (n=60) and double door group (n=60). The single door group underwent single door laminoplasty. The double door group underwent double door laminoplasty. The blood loss,length of stay, complication rate, neurological function improvement, motion range of cervical vertebrae and imaging changes were compared between the two groups.
RESULTS AND CONCLUSION:(1) Blood loss was significantly less, length of stay was significantly shorter, and the incidence of axial symptom was significantly reduced in the double door group than in the single door group (alP< 0.05). (2) No significant difference in complication rate such as side leakage of cerebrospinal fluid, hematoma, infection and paralysis was detected between the two groups (P> 0.05). The incidence of axial symptoms was significantly less in the double door group than in the single door group (P< 0.05). (3) There were no significant differences in preoperative Japanese Orthopaedic Association scores, postoperative Japanese Orthopaedic Association scores, and improvement in neurological function between the two groups (P> 0.05). Postoperative Japanese Orthopaedic Association scores were significantly increased as compared with that preoperatively in both groups (P<0.05). (4) No significant difference in motion range of cervical vertebrae, inflexion, extension angle and sagittal diameter of spinal canal was detectable between the two groups (P> 0.05). Motion range of cervical vertebrae, extension angle, and inflexion angle were smaler after treatment compared with that preoperatively in both groups (P< 0.05). The increased degree of sagittal diameter of spinal canal was smaler, and the motion range of cervical vertebrae kept better in the double door group than in the single door group. (5) These results indicate that the effect of double door laminoplasty for repair of multilevel cervical myelopathy was significant. The double door laminoplasty can shorten the length of stay, reduce blood loss, axial symptoms, and loss rate of motion range of the cervical vertebrae. In the clinic, treatment can be carried out according to different indications.

Objective To investigate the clinical treatment of brucellosis spondylitis (BS) through combination of Mongolian medicine and Western medicine.Methods From December 2010 to June 2015,51 patients which were diagnosed BS at the Department of Brucellosis Hulunbuir People's Hospital were enrolled in this study.After injecting etimicin sulfate and levofloxacin for two weeks,rifampin and doxycycline were administrated orally with Silver pearl pill,Pearl pill and Yunxiang pill (tid) for one treatment cycle which contained three courses of each treatment for 3 weeks.Before and after the treatment,the clinical features and imaging examination results were observed of 2 cases and then followed up for one year.Results Before the treatment,any spinal segment can be affected especially the lumbar spine.Two cases of cervical damage,5 cases of thoracic vertebrae,47 cases of lumbar spine,2 cases of sacral vertebrae,and 39 cases of vertebral abscess were found.After treatment,low back pain symptoms disappeared in 10 cases of the patients.Thirty-nine patients with low back pain symptoms and limited movement.Two patients with low back pain aggravated,MRI examination of the lumbar spine showed lesions increased and abscess formation.The effective rate of Mongolian herbal medicine and Western medicine treatment was 96.1％ (49/51).Conclusion The combined treatment of BS through combination of Mongolia medicine and Western medicine is effective.

BACKGROUND: As a kind of polypeptide, angiotensin-converting enzyme (ACE) inhibitory peptide can lower the blood pressure of human body through restraining the formation of angiotensin Ⅱ.OBJECTIVE: To investigate the influence of AGE inhibitory peptide on endothelial cell proliferation and endothelin expression in cultured human umbilical vein cells based on cellular and molecular levels in order to provide the experimental evidences for ACE inhibitory peptide to be the potential blood pressure-lowering health food.DESIGN: Repeated measures design.SETTING: School of Bioscience and Bioengineering, South China University of Technology; School of Applied Chemistry and Biological Technology,Shenzhen Polytechnic.MATERIALS: The experiment was carried out in the Institute of Applied Chemistry and Biological Technology, Shenzhen Polytechnic from September 2004 to March 2005. The AGE inhibitory peptide was provided by the Institute of Applied Chemistry and Biological Technology, Shenzhen Polytechnic. Under certain circumstance, 15 μ mol/L of the inhibitor was needed to decrease half of the AGE activity. The human umbilical vein endothelial cells of the 4th generation were cultured randomly in 7 groups with different concentrations: medium group, 150, 300 and 600 mg/L ACE inhibitory peptide groups, captopril group, norepinephrine(NE) group, and ACE inhibitory peptide+NE group.METHODS: ①The endothelial cells were cultured as recommended. The medium was M199+FBS(0.15, v/v)+penicillin(10 000 U/mL)+streptomycin (100 mg/L). After cellular fusion, the cells were carried on the passage with the ratio of 1:2. The 4th generation cells were used for experiment. ②M199(0.15, v/v) was contained in each group. ACE inhibitory peptides were added to make the final concentration 150, 300 and 600 mg/L in the 150, 300 and 600 mg/L ACE inhibitory peptide groups respectively. Captopril was added to make the final concentration 10-5 mol/L in the captopril group. NE was added to make the final concentration 100 μg/L in the NE group. ACE inhibitory peptide and NE were added to make the final concentration 300 mg/L and 100 μg/L in the ACE inhibitory peptide+NE group respectively. ③The state of cell growth was determined with cytometry. The contents of endothelial cells in the medium with different culture times were determined with radioimmunoassay. The expression of endothe lin mRNA was determined with reverse transcriptase-polymerase chain reaction. The expression of cellular endothelin protein was determined with immunohistochemical method.MAIN OUTCOME MEASURES: ①The influence of ACE inhibitory peptide on endothelial cell proliferation. ②The influence of ACE inhibitory peptide on the endothelin mRNA and endothelin protein.RESULTS: ①The influence of ACE inhibitory peptide on endothelial cell proliferation and endothelin secretion: Compared with the medium group,in the captopril and 150, 300, 600 mg/L ACE inhibitory peptide groups,the growth of endothelial cells was restrained and the endothelin content in the medium was lowered(P ＜ 0.01 or 0.05). NE could promote the growth of endothelial cells and the secretion of endothelin, but the cell density and endothelin content after treatment with ACE inhibitory peptide were similar to those in the medium group (P ＞ 0.05). ②The influence of ACE inhibitory peptide on the expressions of endothelin mRNA and protein in endothelial cells: Compared with the medium group, the expressions of endothelin mRNA and protein might be lowered in the captopril and 150,300, 600 mg/L ACE inhibitory peptide groups(P ＜ 0.01 or 0.05). The expressions of endothelin mRNA and protein could be up-regulated by NE.The gene expression after treatment with ACE inhibitory peptide was similar to that in the medium group(P ＞ 0.05).CONCLUSION: The ACE inhibitory peptides of different dosages can all restrain the growth of endothelial cells, lower the endothelin content, decrease the expression of endothelin gene and resist NE improved growth and secretion of endothelial cells in umbilical vein cell effectively.