Objective:To explore the effect of polycylic aromatic hydrocarbons (PAHs)on plasma monoamine neurotransmitters. Methods:80 healthy adult male coke oven workers were selected from a coke plant of a state-owned steel enterprise,80 controls were selected from the oxygen factory. Concentration of benzo[a]pyrene(B[a])P in the working environment was monitored by high performance liquid chromatography(HPLC)with fluorescence detector. The level of urinary 1-hydroxypyrene(1-OHP)was determ ined by HPLC with fluorescence detector.The plasma noradrenaline(NE),adrenaline(AD),dopamine(DA)and serotonin(5-HT)levels were measured by high performance liquid chromatography (HPLC) with electrochemical detection. Results:In the working environment of B[a]P, concentration in coke oven workers were significantly higher than controls (P

ObjectiveTo explore the nursing students'puzzles about career planning,and provide a basis for the training.MethodsA qualitative research was conducted on 14 university nursing interns by in-depth interview and observing.The whole interview was noted.The data was analyzed according to Colaizzi's phenomenological procedure.ResultsThe experience could be classified into four aspects,including hazy understanding of career planning,lack of understanding of themselves,limited ca reer identity and lack of social cognition.ConclusionNursing educators should enhance the training against the nursing students'puzzles and make sure they can make a good career planning.

Objective To investigate the changes of detection rates of IL-2,IL-6 and serum anti-Chlamydia immunodominant protein antibody in the patients with Chlamydia trachomatis genital tract infection.Methods Sixty women with genital tract infection (CT group) and 60 healthy women (control group) were selected in our hospital from January to May 2016.The serum levels of IL-2,IL-6 and anti-Chlamydia immunodominant protein and IL-2 and IL-6 levels in cervical secretions were detected in the two groups.Results The IL-2 and IL-6 levels of serum and cervical secretions in the CT group were significantly higher than those in the control group,the difference was statistically significant (P<0.05);the positive detection rates of serum OmcBc antibody,Pgp3 antibody,HSP60 antibody and CTT841 antibody in the CT group were 45.00%,75.00%,23.33% and 38.33% respectively,which were significantly higher than those in the control group,the difference was statistically significant (P<0.05).Conclusion The positive detection rates of IL-2,IL-6 and serum anti-Chlamydia immunodominant protein antibody in the patients with genital tract Chlamydia infection are significantly increased,which has some guiding significance for clinical diagnosis and treatment.

Objective: To study the effects of different restorati ve materials on the health of periodontal tissue. Methods: A total of 40 posterior teeth were divided into four groups with 10 in each. Sil ver amalgam, glass ionomer cements, GlasIonomer Cement FX and light curing Mic roglass composite resin were used to restore Class Ⅱ cavity in each tooth of th e 4 groups respectively . 6 months after restoration gingival cervical fluid (GC F) was collected , GCF aspartate aminotransferase (GCF AST) level was tested a nd plaque index was assessed for each case. Results: The s ilver amalgam and light curing Microglass composite resin groups presented less amount of GCF ( P

Objective To explore the clinical features diagnosis and treatment of left main coronary artery (LM) disease. Methods According to the results of coronary angiography,significant stenosis was defined as ≥50% stenosis. Isolated LM group had 9 patients (4.62%),one-vessel stenosis LM group 15 patients (7.69%),two-vessel stenosis LM group 53 patients (27.18%) and three-vessel stenosis LM group 118 patients (60.51%). Results 195 cases (6.74%) of LM stenosis were found. The incidence rate was low. 164 patients (84.1%) had unstable angina,and 125 patients (64.1%) had myocardial infarction. Coronary artery bypass surgery was performed in 57 patients (29.2%) and the angina disappeared in most of them (84.2%). Five patients received unprotected LM stenosis and angina disappeared in all. Conclusion LM stenosis has sever symptoms. Coronary angiography is the only way for diagnosis and CABG the best treatment. Unprotected LM stenosis is valuable for some patients.

OBJECTIVE:To develop a HPLC assay for the determination of 7 kinds of amino acids in amino acid capsules by using online derivation and diode array detector.METHODS:The high performance chromatograph of liquid with the device of automatic derivation was employed as the automatic sampler.The samples were injected in the predefined procedure,and detected with the diode array detector.RESULTS:Good resolution of 7 kinds of amino acids was obtained,and the respective detectable concentration showed a good linear relationship with its peak area.CONCLUSION:The present method is convenient,well reproduced, highly sensitive,and accurate,therefore it can be widely used.

Objective To investigate effect of applying nursing flowchart in the post-anesthesia care unit(PACU).Method The nursing flowchart in PACU are as follous,including a ventilator connection,monitoring instrument connection,handover of pipeline and skin and training for PACU nurses.Results After application of the nursing flowchart,the nursing time for postoperative patients was reduced from 7.0 min to 4.0 min.No nursing risks and adverse events occurred.Conclusion Application of nursing flowchart in PACU can improve the quality of nursing and enhance patients safety.

Preoperative portal vein embolization(PVE)has become an important tool in the management of selected patients with hepatic cancer before the major hepatic resection is carried out.PVE can redirect the portal flow to the intended future remnant liver tissue in order to induce the hypertrophy of the non-diseased portion of the liver and thereby may reduce the occurrence of complications and shorten the hospitalization days after surgery.This article aims to review the technical and clinical considerations in performing PVE and to discuss the PVE-related practical points,including the relevant anatomy,the access approach,the choosing of embolic agents and the pathophysioiogy of PVE.In addition,the indications and contraindications for performing PVE,the use of combination therapies and the concern for tumor growth after PVE are also discussed.

Objective To discuss the clinical application of feeding-artery embolization in treating massive hemoptysis.Methods The feeding-artery angiography was performed in 72 patients with massive hemoptysis.Based on the angiographic findings polyvinyl alcohol(PVA)or spring coil were selected as the embolization materials.The therapeutic results were retrospectively analyzed. Results Hemoptysis was completely controned almost immediately after the embolization procedure in 46 cases,while it was obviously alleviated in 13 cases.In 11 cases the hemoptysis disappeared completely after 2-4 times of embolization treatment,and in 2 cases surgery had to be employed.Conclusion Embolization of feeding-artery with PVA particles or spring coils is an effective and safe treatment for massive hemoptysis.The key point for decreasing reoccurrence is to occlude all feeding.arteries as far as possible.

Objective To observe the effects of ischemic postconditioning (I-postC) on lung injury after limb ischemia reperfusion (LIR) in rats, and to investigate the protective effect and the mechanisms. Methods Twenty-four Wistar rats were divided into three groups:control group (group Control), ischemia-reperfusion group (group IR) and ischemic postcondi?tioning group (group I-postC). Referring to routine method in our department, the model rats underwent 4-hour ischemia and 4-hour reperfusion of hind limbs were made. In group Control, the rubber band around the limb was loose,which did not block the blood flow. Rats in group I-postC were given repeated 3 times of 5 min ischemia-5 min reperfusion, and then did perfusion 4 h before reperfusion. The blood and lung samples were collected for detecting arterial gas of partial pressure of oxygen [p(O2)] and partial pressure of carbon dioxide [p(CO2)]. The plasma and lung tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD) and xanthine oxidase (XOD) were detected. The morphological changes of lung tissue were ob?served under light microscope and electron microscope. Results It was found that after suffering from ischemia-reperfu?sion, levels of p(O2) and p(CO2) decreased significantly. The activity of SOD in plasma and lung tissues decreased, but XOD and MDA increased significantly (P<0.05). With microscope, lung interstitial vascular dilation, infiltration of neutrophils, the width of the alveolar space, alveolar septal thickening and alveolar exudate were found. Compared with IR group, it was found that p(O2) and p(CO2) increased significantly in group I-postC. The activity of SOD in plasma and lung tissues in?creased, but XOD and MDA decreased significantly(P<0.05). The mild damage of pathological changes were found. Conclu?sion Ischemic postconditioning can reduce the lung injury after limb ischemia reperfusion in rats, which may be related to the inhibition of lipid peroxidation.