Hightiter anti-idiotypic antibodies(Ab_2)sera and two monoclonal anti-idiotypic antibodies (McAb_2)were repared by immunizating syngeneic BALB/c mice wth a complex of 7A_4 monoclonalantibody(McAb_1)against the a eterminant of the Hepatitis B Surface antiger(HBsAg)。Both Ab_2sera and McAb_2 recognize the idiotype of aratope n 7A_4McAb_1.Ab_2 sera contain the antibodiesbinding specially to anti-HBs from other species(guinea pig、onkey、rabbit and goat)and the twoMcAb_2 can not bind to these anti-HBs.Therefore it is thought that the two McAb_ re noninternalimage(Ab_2?)anti-idiotypic antibodies,this study proves that the McAb_1 against he eterminantof the HBsAg are different in their paratope,The McAb_2 can be used to stinguish the parotope onthe McAb_1 as a biological probe.

Percutaneous mechanical circulatory support (PMCS) devices are effective in the treatment of patients with cardiogenic shock and acute decompensated heart failure, as a bridge to the recovery of heart function or further treatment. The intra-aortic balloon pump (IABP) is now the most widely used PMCS. New PMCS devices including Transseptal device of Tandem-Heart, transaortic valve device of Impella and extracorporeal membrane oxygenation (ECMO) might provide more effective hemodynamic supports. Doctors should choose appropriate PMCS devices and their working modes, according to patient's clinical conditions, based on the working principles and hemodynamic effects of the devices, in order to achieve the best effects and help patients live through the crisis of diseases.

Humanistic quality of medical workers directly affects the quality of medical service and patient satisfaction.In view of patient′s value demand,this paper identified main setbacks in humanistic service in China in the course of medical service.The authors found out the reasons for the lack of humanities quality,and interpreted medical humanistic spirit,medical professional spirit,medical ethics and morality,and connotation of medical law culture.On such basis,the paper unveiled the importance of medical humanities accomplishment in medical practice and medical students′training,proposing to promote the concept of humanistic spirit of medicine and the doctor-patient communication skills.

Objective To find out the factors which influence the establishment of collateral circulation as well as its clinical characteristics by analyzing the sufferers' medical records.Methods Review and collect the records of the first coronary angiography,and we can find the total occlusion of one coronary after coronary angiography.There are 52 cases in which people who suffered the establishment of collateral circulation stay in hospital for treatment.Here,we take 52 cases in which people didn't suffer into comparison and analyze these clinical records.Results Use the Logistic regression method to analyze the relationship between general clinical data,past history,habits,current medication and the establishment of collateral circulation,which relates to the diabetes mellitus anamnesis,exercise and drinking habits,with the regression coefficient is 0.408,0.483,0.197(P

Network theory is applied to the optimization study on field medical equipment allocation with patient flow and its rate in wartime analyzed. With the application of queuing theory and linear programming, different optimized combinations of evacuation tools are studied, and then static model and dynamic model are established. A calculating table on queue length, service intensity and service desk is put forward to realize rapid evaluation of quantities of required field medical equipment for PLA. Some suggestions are provided to modify the current standard of PLA's basic medical equipment.

[Summary] A 60-year-old man was admitted with main complaint of chest pain on exertion.Coronary angiogram showed severe triple vessel disease.He underwent the hybrid technique of treatment through two left minithoracotomy incisions.Both internal thoracic arteries were harvested under direct vision.The left internal mammary artery (LIMA)and right internal mammary artery (RIMA) compositing Y grafts was constructed. The LIMA was anastomosed to the left anterior descending branch and the RIMA was anastomosed to the ramus intermedius.The operation time was 306 min.The amount of bleeding during operation was 50 ml.Five days later he underwent percutaneous coronary intervention at right coronary artery lesion successfully.He was discharged from hospital 8 days after operation,and didn’t complain of chest pain during fellow-up for one month.This technique demonstrates to be an effective and minimally invasive alternative to treat patients with multivessel coronary artery disease.

Objective To compare the diagnostic efficiency in showing the responsible blood vessels for neurovascular compression in patients with trigeminal neuralgia by 3D‐FIESTA‐C and 3D‐TOF‐MRA sequences .Methods The imaging data of 60 patients with primary trigeminal neuralgia were analyzed retrospectively .After MRI examination ,all of the patients underwent micro‐vascular de‐compression (MVD) .3D‐TOF‐MRA and 3D‐FIESTA‐C sequences were performed to evaluate the three‐dimensional relationship be‐tween trigeminal nerve and blood vessels through the original and reconstructed image .The intraoperative endoscopic findings were set as the gold standard comparing to the manifestations of 3D‐TOF‐MRA and 3D‐FIESTA‐C .Results The sensitivities of 3D‐TOF‐MRA and 3D‐FIESTA‐C for the diagnosis of the existence of responsible vessels were 85 .7% ,89 .3% ,the specificities were 75 .0% , 100% ,and the accuracies were 85 .0% ,90 .0% ,respectively (P=1 .000) .Furthermore ,the sensitivities of 3D‐TOF‐MRA and 3D‐FIESTA‐C for the diagnosis of the existence of responsible arteries were 94 .1% ,88 .2% (P=0 .244) ,while the sensitivities of the responsible veins were 0 .00% and 88 .2% (P=0 .009) .Conclusion Both the 3D‐FIESTA‐C and 3D‐TOF‐MRA sequences can accurately deter‐mine the existence of responsible vessels in trigeminal neuralgia before surgery .3D‐FIESTA‐C sequence is superior to 3D‐TOF‐MRA for presenting the responsible veins ,which can be used as a supplemental diagnostic tool before operation .

Therapeutic alliance is one of the most popular topics in psychological counseling and psychotherapy research,but the concept of it is not unified.Despite of its various versions,most scholars regard it as an important relationship variable,emphasizing the agreement between therapist and patient as to the goals and tasks of treatment,and the affective bond established in the therapeutic dyad.Related research show that the characteristics of therapist and patient,and the interaction in this therapeutic dyad are the related factors on the formation and development of therapeutic alliance,which constitutes a major clinical variable in explaining and predicting the course and outcome of treatments.Future researches should further clarify the related factors of therapeutic alliance,expand the clinical applications of therapeutic alliance and strengthen the exploration of indigenization.

Objective: To compare the phenotype of induced dendritic cell (DC) derived from peripheral blood of the healthy individuals and the patients with oral carcinoma. Methods:15 patients with oral carcinomal at stages II or above were assigned to study group and 15 cases of healthy volunteers were assigned to control group. Leukocyte were isolated by density centrifugation. Adherent fractions were incubated in present of GM-CSF,IL-4 and TNF-? for 7 days in vitro. The morphological change of the cultured cells was observed by light microscopy and electronic microscopy. The phenotype expressions of CD83,CD1a,CD86, CD80, HLA-DR were analysized by flow cytometry. Results:Typical DCs were obtained from two groups after 7 days culture. The expression rate of CD83,CD1a on DC from healthy volunteers was significantly higher than that from patients (P0.05). Conclusion:DC derived from oral cancer patient' s peripheral blood monocyte might be more matured and is a good cell model for further immunotherapy and research.

Objective To establish a method for determination of tellurium(Ti)in the air of workplace by microwave digestion-HG-AFS. Methods The air samples were collected by micropore filters and the concentration of tellurium was determined by microwave digestion-HG-AFS. Results In this method, the linear range was 0.11-40.0 ?g/L, the detection limit was 0.11 ?g/L, the lowest detected concentration of tellurium was 0.000 09 mg/m3(based on 30 L air sample), the rate of recovery was in the range of 95.5%-98.2%, the RSD was less than 3%. Conclusion The method is rapid, accurate, sensitive and suitable for the determination of tellurium in the air of workplace.