Hightiter anti-idiotypic antibodies(Ab_2)sera and two monoclonal anti-idiotypic antibodies (McAb_2)were repared by immunizating syngeneic BALB/c mice wth a complex of 7A_4 monoclonalantibody(McAb_1)against the a eterminant of the Hepatitis B Surface antiger(HBsAg)。Both Ab_2sera and McAb_2 recognize the idiotype of aratope n 7A_4McAb_1.Ab_2 sera contain the antibodiesbinding specially to anti-HBs from other species(guinea pig、onkey、rabbit and goat)and the twoMcAb_2 can not bind to these anti-HBs.Therefore it is thought that the two McAb_ re noninternalimage(Ab_2?)anti-idiotypic antibodies,this study proves that the McAb_1 against he eterminantof the HBsAg are different in their paratope,The McAb_2 can be used to stinguish the parotope onthe McAb_1 as a biological probe.

Percutaneous mechanical circulatory support (PMCS) devices are effective in the treatment of patients with cardiogenic shock and acute decompensated heart failure, as a bridge to the recovery of heart function or further treatment. The intra-aortic balloon pump (IABP) is now the most widely used PMCS. New PMCS devices including Transseptal device of Tandem-Heart, transaortic valve device of Impella and extracorporeal membrane oxygenation (ECMO) might provide more effective hemodynamic supports. Doctors should choose appropriate PMCS devices and their working modes, according to patient's clinical conditions, based on the working principles and hemodynamic effects of the devices, in order to achieve the best effects and help patients live through the crisis of diseases.

Humanistic quality of medical workers directly affects the quality of medical service and patient satisfaction.In view of patient′s value demand,this paper identified main setbacks in humanistic service in China in the course of medical service.The authors found out the reasons for the lack of humanities quality,and interpreted medical humanistic spirit,medical professional spirit,medical ethics and morality,and connotation of medical law culture.On such basis,the paper unveiled the importance of medical humanities accomplishment in medical practice and medical students′training,proposing to promote the concept of humanistic spirit of medicine and the doctor-patient communication skills.

Objective To find out the factors which influence the establishment of collateral circulation as well as its clinical characteristics by analyzing the sufferers' medical records.Methods Review and collect the records of the first coronary angiography,and we can find the total occlusion of one coronary after coronary angiography.There are 52 cases in which people who suffered the establishment of collateral circulation stay in hospital for treatment.Here,we take 52 cases in which people didn't suffer into comparison and analyze these clinical records.Results Use the Logistic regression method to analyze the relationship between general clinical data,past history,habits,current medication and the establishment of collateral circulation,which relates to the diabetes mellitus anamnesis,exercise and drinking habits,with the regression coefficient is 0.408,0.483,0.197(P

Network theory is applied to the optimization study on field medical equipment allocation with patient flow and its rate in wartime analyzed. With the application of queuing theory and linear programming, different optimized combinations of evacuation tools are studied, and then static model and dynamic model are established. A calculating table on queue length, service intensity and service desk is put forward to realize rapid evaluation of quantities of required field medical equipment for PLA. Some suggestions are provided to modify the current standard of PLA's basic medical equipment.

Objective: To compare the phenotype of induced dendritic cell (DC) derived from peripheral blood of the healthy individuals and the patients with oral carcinoma. Methods:15 patients with oral carcinomal at stages II or above were assigned to study group and 15 cases of healthy volunteers were assigned to control group. Leukocyte were isolated by density centrifugation. Adherent fractions were incubated in present of GM-CSF,IL-4 and TNF-? for 7 days in vitro. The morphological change of the cultured cells was observed by light microscopy and electronic microscopy. The phenotype expressions of CD83,CD1a,CD86, CD80, HLA-DR were analysized by flow cytometry. Results:Typical DCs were obtained from two groups after 7 days culture. The expression rate of CD83,CD1a on DC from healthy volunteers was significantly higher than that from patients (P0.05). Conclusion:DC derived from oral cancer patient' s peripheral blood monocyte might be more matured and is a good cell model for further immunotherapy and research.

Objective To explore the relationship of the number of balloon inflations during primary percutanoeus coronary intervention(PCI) with myocardial perfusion and the extent of myocardial necrosis,and to identify the potential clinical efficacy of ischemic postcondioning in patients undergoing Primary PCI.Methods One hundred and fourty patients with ST-segment elevation myocardial infarction(STEMI) who underwent primary PCI were eligibly enrolled for retrospective analysis.The infarct-related artery(IRA) in all patients had to be either left anterior descending artery(LAD) or right coronary artery(RCA) with TIMI 0 flow grade and no collateral filling prior to intervention,and had to be completely reperfused with TIMI Ⅲ flow grade after PCI.Patients with cardiac arrest,cardiogenic shock,previous MI and pre-infarct angina were excluded.Patients were divided into two groups for analysis based on the number of balloon inflations performed during primary PCI:Group A included all patients in whom 1-2 inflations were applied,while patients in Group B received 3 or more inflations.Myocardial perfusion was evaluated by myocardial blush grade and ST-segment resolution.The extent of myocardial necrosis was evaluated by peak values of CK and CK-MB release and predischarge left ventricular ejection fraction(LVEF) measured by echocardiogram.Results The 2 groups showed similar baseline characteristics,while patients in Group B received more balloon inflations than those in Group A(4.7?0.9 vs.1.8?0.2,P0.05),but decreased to 1.65?0.49 mm and 0.98?0.37 mm(P

Therapeutic alliance is one of the most popular topics in psychological counseling and psychotherapy research,but the concept of it is not unified.Despite of its various versions,most scholars regard it as an important relationship variable,emphasizing the agreement between therapist and patient as to the goals and tasks of treatment,and the affective bond established in the therapeutic dyad.Related research show that the characteristics of therapist and patient,and the interaction in this therapeutic dyad are the related factors on the formation and development of therapeutic alliance,which constitutes a major clinical variable in explaining and predicting the course and outcome of treatments.Future researches should further clarify the related factors of therapeutic alliance,expand the clinical applications of therapeutic alliance and strengthen the exploration of indigenization.

To find out the risk factors of hospital infection in childhood primary nephrotic syndrome(nephrosis) and prophylactico therapeutic measures, 190 cases of child patients hospitalized for nephrosis during the period lasting from 1991 to 1999 were collected. Then a retrospective analysis of the distribution of the various risk factors was made using the single factor analysis and the logistic multi factor regression model. The hospital infection rate was found to be 34.2%, with respiratory infection accounting for 69.7%. The single factor analysis indicated that the risk factors included quantity of urinary protein per kilogram of weight within 24 hours(urinary protein), length of stay, length of hormone usage, entities of antibiotics used and length of such usage. The multi factor analysis indicated that the risk factors included urinary protein, entities of antibiotics used and length of such usage. The authors present the view that strengthening the control of respiratory diseases, setting up clean wards, and ensuring the rational use of antibiotics are some of the key measures for reducing the rate of hospital infection. The quantity of urinary protein is a sensitive index of hospital infection in nephrosis. Once diagnosis of nephrosis has been confirmed, it is necessary to use ACH in full dose and for a complete course of treatment so as to reduce the loss of protein from the urine as soon as possible.

Objective To compare the diagnostic efficiency in showing the responsible blood vessels for neurovascular compression in patients with trigeminal neuralgia by 3D‐FIESTA‐C and 3D‐TOF‐MRA sequences .Methods The imaging data of 60 patients with primary trigeminal neuralgia were analyzed retrospectively .After MRI examination ,all of the patients underwent micro‐vascular de‐compression (MVD) .3D‐TOF‐MRA and 3D‐FIESTA‐C sequences were performed to evaluate the three‐dimensional relationship be‐tween trigeminal nerve and blood vessels through the original and reconstructed image .The intraoperative endoscopic findings were set as the gold standard comparing to the manifestations of 3D‐TOF‐MRA and 3D‐FIESTA‐C .Results The sensitivities of 3D‐TOF‐MRA and 3D‐FIESTA‐C for the diagnosis of the existence of responsible vessels were 85 .7% ,89 .3% ,the specificities were 75 .0% , 100% ,and the accuracies were 85 .0% ,90 .0% ,respectively (P=1 .000) .Furthermore ,the sensitivities of 3D‐TOF‐MRA and 3D‐FIESTA‐C for the diagnosis of the existence of responsible arteries were 94 .1% ,88 .2% (P=0 .244) ,while the sensitivities of the responsible veins were 0 .00% and 88 .2% (P=0 .009) .Conclusion Both the 3D‐FIESTA‐C and 3D‐TOF‐MRA sequences can accurately deter‐mine the existence of responsible vessels in trigeminal neuralgia before surgery .3D‐FIESTA‐C sequence is superior to 3D‐TOF‐MRA for presenting the responsible veins ,which can be used as a supplemental diagnostic tool before operation .