Objective To explore the effects of hypoxic post-conditioning on cognitive function and the expression of silent information regulator 1 (SIRT1) in hippocampal CA1 of rats with cerebral ischemia. Methods Sixty Sprague-Dawley rats were randomly divided into sham operation group, model group and treatment group with 20 cases in each group. Each group was divided into one day, two days, three days, seven days subgroups according to the time of ischemia reperfusion. Global cerebral ischemia reperfusion was induced with modified Pulsinelli′4-vessel occlusion. The treatment group received 8%oxygen for two hours after ischemia. The cognitive function was assessed with Morris water maze test. Morphological changes of the hippocampal CA1 region were observed by HE staining. The expression of SIRT1 in the hippocampal CA1 region was detected with immunohistochemical assay and Western blotting. Results Compared with the model group, the escape latency significantly shortened (P<0.05), the number of times crossing the platform increased (P<0.05), the speed and the percentage of time spent in the platform quadrant increased (P<0.05), and the total distance decreased (P<0.05);the expression of SIRT1 in hippocampal CA1 increased (P<0.05) and the number of normal neurons increased (P<0.05) in the treatment group. Conclusion Hypoxic post-conditioning can improve the cognitive function of rats with global cerebral ischemia, which may relate with up-regulating SIRT1 in hippocampus.

Objective To explore the application of related parameters of frailty index,and use this method to evaluate the prognosis of respondents.Methods Evaluations based on the utilization of frailty index was performed in a cohort of 1808 elderly people aged 60 years and above who dwelled in Beijing urban and rural area.And the baseline of this survey started in 2004,and the follow-up procedure was performed at the end of third,fifth and eighth years.The mortality of all individuals was collected and analyzed.Results Along with the increasing age,higher frailly index was expected(r=0.314、0.395、0.405、0.319,P=0.004、0.000、0.000、0.000).Among the same level of frailty index,males,rural dwellers,and elderly people showed a higher death rate than females,urban dwellers and comparatively young individuals.Conclusions The frailty index may be a significant tool for evaluation of prognosis among elderly people.

Objectives To explore the status and characteristics of changes in lung function in the elderly in communities of Beijing,and to provide reference for improving the prevention and control of chronic lung disease in the elderly.Methods A stratified,segmented and cluster sampling method was used to investigate 1 144 subjects aged 60 years and over in communities of Beijing (Huairou District,Daxing District rural area and Xicheng District).Health status and pulmonary function were assessed,and characteristics of lung functions and their changes were analyzed in the elderly.Results The average vital capacity(VC)was 2.53L,and forced vital capacity(FVC) was 2.29 L,forced expiratory volume in one second (FEV1)was 1.81 L,1 second rate (FEV1/FVC) was 83.42 ％.Static vital capacity was decreased in 1/3 of the elderly,and dynamic vital capacity,including FVC and FEV1,was reduced in about half of the elderly.Ratio of decline in lung function in the elderly was higher in male and in urban area than in women and in rural areas.Ratio of decline in each index of pulmonary ventilation function showed a significantly increased tendency along with the ageing.Lung dysfunction types in the elderly included a restricted (23.5％)and obstructive ventilation disorder (19.7％)and mixed ventilation disorder(12.8％).Conclusions The ratio of decline in lung function in the elderly is increased along with aging,and more significantly higher in female and in urban than in male and in countryside.Restrictive ventilation disorder in the elderly is more often in urban areas,while obstructive ventilation disorder occurs mostly in rural areas.Corresponding measures should be adopted for different groups of people.

Objective To study the association of prevalence of cardio-cerebro vascular diseases (CCVD) with metabolic syndrome (MS) defined by different criteria in middle and aged people.Methods A cross-section survey was conducted among 1458 people aged 55 years and over in urban and rural areas of Beijing in 2009.MS definitions by World Health Organization (WHO),National Cholesterol Education Program-Adult Treatment Panel Ⅲ (NCEP-ATP Ⅲ ) revised,international diabetes mellitus (IDF) and Chinese Diabetes Society (CDS) were applied to analyze the association MS with the prevalence of CCVD.Results The risks of CCVD were all significantly higher in patients with MS than with non-MS using 4 definitions of MS.The definitions of WHO and CDS showed well agreement with the prevalence of CCVD.OR values of MS for CCVD were 2.14 folds (95％ CI:1.59-2.87) for WHO definition and 1.91 folds (95％ CI:1.43-2.55) for CDS definition,while OR values of MS for CCVD were 1.68 folds (95％ CI:1.32-2.15) for NCEP-ATPⅢ definition and 1.64 folds (95％ CI:1.26-2.13) for IDF definition as compared to non-MS,respectively.After adjustment for age,sex,region,history of smoking and alcohol drinking,the OR values in above four definitions were 1.91,1.88,1.67 and 1.80,respectively.The OR value of MS was highest for stroke by CDS definition and highest for coronary heart disease by WHO definition.Conclusions MS values defined by WHO and CDS are the optimal index of prevalence for CCVD in middle and aged people.

OBJECTIVETo investigate the influence of intraoperative implantation of radioactive (125)I seeds on healing of surgical anastomosis.

METHODSThe jejunum was cut, and end-to-end anastomosis was made in 12 healthy dogs. In the experimental group (n = 8), the (125)I seeds were implanted into the two sides of the anastomosis. The total radiation dosage at the anastomosis was 116 Gy. The other 4 dogs were in the included control group. At the 7th, 14th postoperative day, specimens were obtained from 4 dogs in the experimental group and 2 dogs in the control group respectively. The healing of the anastomosis was observed grossly; hydroxyproline content, as well as histopathological and ultrastructural changes of the anastomotic tissue were studied.

RESULTSGross observation showed healing of the anastomosis of the experimental animals. The hydroxyproline contents were 0.578 +/- 0.020 microg/mg proteins in the experimental group and 0.631 +/- 0.012 microg/mg proteins in the control group (P > 0.05). Histopathological and ultrastructural changes of the anastomotic tissue were not significant in healing as compared to the control group. One of 29 patients had anastomotic leakage.

CONCLUSIONSIntraoperative implantation of (125)I has no adverse effect on healing of surgical anastomosis; it is safe and feasible in clinical practice.

Adult ; Aged ; Anastomosis, Surgical ; Animals ; Brachytherapy ; Dogs ; Female ; Humans ; Intraoperative Care ; Iodine Radioisotopes ; pharmacology ; therapeutic use ; Male ; Middle Aged ; Models, Animal ; Neoplasms ; drug therapy ; Wound Healing ; drug effects

OBJECTIVETo study the relationship between body mass index (BMI), hypertension and all-cause mortality in the elderly population in Beijing.

METHODSIn 1992, a cohort of 3 257 people above 55 years old was selected from 3 different areas of Beijing, i.e. urban and suburbs (both plain and mountain areas). Information on physical condition, history of chronic diseases, self-perceived health, as well as smoking and drinking status was collected. Blood pressure, body height and weight were measured among 2 086 of 3 257 subjects.

RESULTSBMI was decreasing with age, while the prevalence of hypertension increased with age. Meanwhile the prevalence of hypertension increased with age as well as with BMI. There was a reverse relationship between BMI and all-cause mortality, i.e. the risk of death was 39.0% lower for those with BMI = 20.0 - 24.9 kg/m(2) (Hazard ratio: 0.61, 95% Confidence interval: 0.49 - 0.75) and 62% lower for those with BMI >/= 25.0 kg/m(2) (Hazard ratio: 0.38%, 95% Confidence interval: 0.29 - 0.49) than those with BMI < 20.0 kg/m(2). After controlling for age, gender, residential place, hypertension as well as self-perceive health status and cognition function, low BMI remained a significant and independent predictor to death.

CONCLUSIONThe distribution of BMI was different in elderly from the youth. The findings suggested that it was important to control the body weight among the elderly hypertensives.

Age Factors ; Aged ; Aged, 80 and over ; Body Height ; Body Mass Index ; Body Weight ; Cause of Death ; China ; epidemiology ; Female ; Humans ; Hypertension ; epidemiology ; physiopathology ; Male ; Middle Aged ; Risk Factors ; Rural Health ; statistics & numerical data ; Sex Factors ; Urban Health ; statistics & numerical data

Objective To verify the analytical performance of Hitachi 7600-210automatic biochemical analyzer detection system.Methods The precision, accuracy, linearity and clinical reportable range, limit of quantitative detection and anti-interference capability were validated according to Clinical and Laboratory Standards Institute (CLSI) documents (EP15-A3and EP17-A2) and Clinical Laboratory Improvement Amendment 1988 (CLIA′88) standards.Results The within precisions of high and low two concentrations were both less than1/4CLIA′88TEa (laboratory permissible total error), the day precisions were less than 1/3CLIA′88TEa, the pass rates of three external quality assessments in 2017 were all not less than 80％and range from 0.02mmol/L to 401.80mmol/L.The clinical reportable was ranged from 0.02to 401.80mmol/L with a linear relationship.The LoB, LoD and LoQ of glucose (GLU) detection were 0.01 mmol/L, 0.03 mmol/L and 0.08mmol/L respectively.The anti-interference capability to hemoglobin (Hb), vitamin C (VitC), bilirubin (BIL) and triglyceride (TG) in the detection system to GLU measurement were in accordance with the manufacturer′s statement.Conclusion Performance verification of Hitachi 7600-210automatic biochemical analyzer detection system to GLU detection is consistent with the manufacturer statement also in accordance with CLIA′88standards, which can meet the expectant use of clinical test.