OBJECTIVE:To develop an HPLC method for simultaneous determination of calycosin-7-O-?-D-glycoside,ono-nin,calycosin and formononetin in Radix Astragali from eleven different habitats and to explore the internal factors of geoherbalism based on aspect of contents of active constituents. METHODS:The sample was separated on Zorbax Eclipse XDB-C18(250 mm?4.6 mm,5 ?m)column. The mobile phase consisted of acetonitrile-0.2% formic acid solution (gradient elution). The UV detection wavelength was set at 280 nm. RESULTS:The linear ranges of calycosin-7-O-?-D-glycoside,ononin,calycosin and formononetin were 0.005 1～0.510 mg?mL-1,0.005 0～0.300 mg?mL-1,0.004 9～0.294 mg?mL-1,0.004 6～0.276 mg?mL-1,respectively(r=0.999 9).Calycosin-7-O-?-D-glycoside and ononin took up a big proportion in Radix Astragali from Inner Mongolia and Shanxi; calycosin and formononetin took up a big proportion in Radix Astragali from Anguo,Chicheng of Hebei province and Dingxi of Gansu province. CONCLUSION:The method is simple,rapid and accurate. Results of study are in line with textual research on Radix Astragali.

Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

Objective　To observe the effect of mycophenolate mofetil (MMF),a new type of immunosuppressant,on the immune function in patiens with systemic lupus erythematosus (SLE).Methods　The changes of serum lL-6,IL-8,TNF-α,sIL-2,ANA,A-dsDNA and subsets of lymphocytes were observed in patients with SLE before and after treatment with MMF and CTX by ELISA,indirect immunofluorescent assay and flow cytometry,and the effect of MMF and CTX on the functions of hematopoiesis,liver and kidney were also observed.Results　Three months after treatment with MMF,the serum levels of IL-6,IL-8,TNF-α,sIL-2,ANA,and A-dsDNA in patients with SLE were significantly decreased,CD3＋,CD4＋ and CD4＋CD45RA＋ cells were significantly increased,and CD8＋,CD4＋CD45RO＋,CD8＋CD45RA＋,CD8＋CD45RO＋ cells were significantly decreased.No remarkable injury was observed on the functions of hematopoiesis, liver and kidney after treatment with MMF.Conclusion　MMF can inhibit T and B lymphocytes selectvely and is a new type of immunosuppresant to treat SLE with less toxicity and side-effect.

Objective To evaluate the effect of heat treatment on ultraviolet B (UVB)-induced oxidative injury to human melanocytes.Methods Melanocytes were isolated from adult foreskins,and subjected to a primary culture.After 3-4 passages of subculture,the melanocytes were classified into 4 groups:control group incubated at 37 ℃,heat treatment group incubated at 42 ℃ for 1 hour,UVB group exposed to UVB irradiation at 100 mJ/cm2,combination group receiving heat treatment at 42 ℃ for 1 hour followed by UVB irradiation at 100 mJ/cm2.After three successive days of treatment,MTT assay was performed to evaluate cell viability,a biochemical method to determine the activity of superoxide dismutase (SOD) and concentration of malonaldehyde (MDA),and flow cytometry to detect intracellular reactive oxygen species (ROS) and apoptosis in melanocytes.Results The cell survival rate,apoptosis rate,SOD activity,MDA concentration and ROS level were (100 ± 6.14)％,(4.66 ± 0.58)％,(53.39 ± 8.23) U/gprot,(1.09 ± 0.32) mmol/gprot,and 1070.85 ± 42.07 in the control group respectively.UVB exposure induced a significant increase in apoptosis rate (24.14％ ± 2.90％,P ＜ 0.001),MDA concentration (1.65 ± 0.33 mmol/gprot,P ＜ 0.01) and ROS level (1416.45 ± 79.12,P＜ 0.01),but a significant decrease in cell survival rate (50.23％ ± 5.36％,P＜ 0.01)and SOD activity (31.98 ± 1 1.89 U/gprot,P ＜ 0.01) in the UVB group compared with the control group,while the heat pretreatment markedly downregulated the UVB-induced increase in apoptosis rate (14.9％ ± 1.49％,P ＜ 0.001),MDA concentration (1.10 ± 0.26 mmol/gprot) and ROS level (1033.30 ± 68.41,P＜ 0.01),as well as the decrease in cell survival rate (74.12％ ± 6.17％,P＜ 0.01) and SOD activity (51.63 ± 6.55 U/gprot,P＜ 0.01) in the combination group.Conclusion Heat treatment could protect melanocytes from UVB-induced oxidative injury.

ABSTRACT:Objective To compare the prognostic value of cystatin-C (Cys-C)and creatinine (Cr)in chronic heart failure (CHF)in old people.Methods We recruited 183 old patients (>75 years)diagnosed with CHF hospitalized at the Department of Cardiology and the Department of Geriatrics of our hospital from 2010 to 2012. Venous blood was withdrawn to detect the expressions of Cys-C and Cr.After three-year follow-up,the patients’ three-year survival was recorded.Logistic regression model was used to examine the relationship of Cys-C,Cr and other risk factors with the CHF patients’mortality.ROC curve was used to compare the prognostic value of Cys-C and Cr in CHF in old people and statistical method was adopted to analyze the combined use of Cys-C and Cr for determining their value in evaluating CHF prognosis.Results At the end of the follow-up,74 (40.44%)patients died.Serum cys-C and Cr levels of the patients in death group increased compared with those in survival group (P<0.05).Multivariate logistic regression showed that age (OR=1.11,95% CI=1.04-1.19),SBP (OR=1.06,95%CI=1.01-1.10),LVEF (OR=0.89,95% CI=0.82-0.95),BNP (OR=4.74,95% CI=1.77-12.69), creatinine (OR=2.04,95% CI=1.03-4.08),and Cys-C (OR=2.97,95% CI=1.44-6.12)were the independent risk factors for the prognosis of CHF.ROC analysis showed that Cys-C was superior to creatinine in AUC value (0.71 vs.0.65;95% CI,0.64-0.78;95% CI,0.58-0.72)(P<0.001).The AUC value of Cys-C and Cr in combination was 0.73 (95% CI,0.66-0.79).Conclusion Cys-C is a stronger predictor of the prognosis of CHF patients than creatinine and the combination of the two can increase the sensitivity and specificity in evaluating the prognosis of patients with CHF.

Objective To investigate the effects of dieting and exercise intervention on lipid level in patients with hyperlipidemia.Methods Health education including dieting and exercise interventions was given to 1,800 patients with hyperlipidemia.Serum total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL-C)and low density lipoprotein (LDL-C)levels were assessed before and after interventions.Result TC, TG and LDL-C levels were decreased and HDL-C increased after interventions with statistical significance(P<0.01).Conclusions Dieting and exercise intervention can be effective in controlling lipid levels effectively.Health education should vary among individuals to achieve good effects.

The 53rd ASH meeting was held in San Diego in December 2011. The meeting has received many advanced reports on the diagnosis and treatment of multiple myeloma (MM).On therapy,with the application of thalidomide, lenalidomide and bortezomib as inductive treatments, the clinical outcomeshave been improved greatly in MM patients.Pomalidomide,a new immunomodulator,can be hopefully used as a frontline medicine for MM because of its high response. Bendamustine combination with other treatments induced efficient response and good response rates in MM patients with renal insufficiency or relapsed/refactory disease. Currently autologous stem cell transplantation is still the standard care for newly diagnosed young MM patients.At this time other kinds of novel agents have entered into clinical trials and have shown a bright future of application.

Objective To explore the effect of swallowing training on dysphagia in patients with head and neck neoplasm receiving ra-diotherapy. Methods 60 patients with head and neck neoplasm receiving radiotherapy from March, 2014 to August, 2015 were divided into intervention group (n=30) and control group (n=30) randomly. The control group received routine nursing and relevant health education, while the intervention group received swallowing training from 2 weeks before to 3 months after radiotherapy. They were assessed with Functional Oral Intake Scale and M. D. Anderson Dysphagia Inventory 1 week, 3 months after radiotherapy. Results The scores of Function-al Oral Intake Scale (Z=-3.195, P<0.01), the total score of M. D. Anderson Dysphagia Inventory and the subscores (t>4.385, P<0.01) were better in the intervention group than in the control group 1 week after radiotherapy, as well as 3 months after radiotherapy (Z=-4.436, P<0.01;t>5.361, P<0.01). Conclusion Swallowing training could improve the condition of dysphagia of head and neck neoplasm patients after radiotherapy.

Objective To explore the effects of alcohol on NO concentration in plasma and activity of ATP enzyme in erythrocytes of rabbits. Methods 32 rabbits were divided into groups and treated with alcohol, injected intravenously, at the doses of 0.5 g/kg, 1.0 g/kg and 1.5 g/kg, 15 minutes after treatment, the blood samples were collected and NO concentration in plasma, activity of ATP enzyme in erythrocytes were determined. Results NO concentration in plasma of rabbits significantly decreased in 0.5 g/kg and 1.0 g/kg groups, but increased in 1.5 g/kg group (P

Objective To explore the effects of the total number of medical staff working in the room and their different activities on the colony population of air bacteria in NICU. Methods To count the colony population of air bacteria under different conditions and analyze the influenced factors . Results The colony population of room air bacteria was well correlated and statistically significant with the number of medical staff working in the room(r=0.78,P