Objective:To analysis the clinical characteristics of pulmonary hypertension (PH) complicating pregnancy, then to explore the delivery time and the monitodng as well as the treatment during perinatal peri-od.Methods:The data of 10 cases with PH treated in our hospital from June 2005 to January 2009 were retro-spectively analyzed.Reaults:1 case was primary pulmonary hypertension(PPH) ; 9 cases were secondary PH (SPH), in which 4 cases were rheumatic heart disease and 5 cases were congenital heart diseases.3 cases were slight PH,6 cases were moderate PH, and 1 were severe group.4 cases NYHA class were Ⅰ～Ⅱ and 6 cases NYHA class were Ⅲ～Ⅳ.4 cases were term delivery,5 cases were premature delivery, and 1 got artifi-cial abortion.8 cases were cesarean section, and 1 was vaginal delivery.2 cases were very low birth weight infants, 1 infant was death and 1 mother was death.Conclusions:The patient of PPH should avoid concei-ving.For SPH patients whether to conceive depends on cardiac function, the level of pulmonary arterial pres-sure and should better get pregnancy after surgery or medication.It should terminate pregnancy in the first tri-mester for moderate or severe patients.Cesarean section is more suitable for those in the second or third tri-mester.The prognosis of the mother and neonate depends on several factors.

BACKGROUND: The recent researches show that there are various kinds of autoantibodies in epilepsy patients such as anti-nuclear antibodies, anticardiolipin antibodies,anti-?2 glucoprotein antibodies and anti-glutamic acid decarboxylase antibodies,which indicate that some types of epilepsy are mediated by autoimmune mechanism.OBJECTIVE: To detect the level of anti-glutamic acid receptor (GluR1)antibody in epilepsy children.DESIGN: A controlled trial.SETTING: Medical College of Dalian University, General Hospital of Jilin Chemistry Group and Sanchong University, Japan.PARTICIPANTS: Totally 56 cases of epilepsy children were from Maternal and Child Health in Qingdao City including 30 males and 26 females and aged from 3 months to 13 years.While 48 health examinees and 12 patients with brain tumor from General Hospital of Jilin Chemistry Group were taken as controls, including 38 males and 12 females and aged from 6months to 17 years.Their guardians were all informed of the detection index and consented to join the study.METHODS: Polystyrene plates were coated with GluR1 polypeptide antigen,and optimal conditions of enzyme linked immunosorbent assay (ELISA) for GluRI antibody was ascertained with chessboard titration.MAIN OUTCOME MEASURES: The GluR1 antibody level was detected with ELISA.RESULTS:The concentration of coating GluR1 polypeptide antigen was20 mg/L; The dilution of serum and horseradish peroxidase sheep anti-hu-man (HRP-SaH) IgG were 1:50 and 1:1 500 respectively. Intra-assay andinter-assay coefficientof variability were 4.48％-8.80％ and 11.18％-16.60％ respectively; The specific absorption test demonstrated that the ab-sorbance value of positive serum decreased 2.4 times after absorption ofGluR1 polypeptide compared with that before absorption.There was obvi-ous higher positive rate of GluR1 antibody in serum of epilepsy childrenthan control group (40％, 5％, P＜0.01).CONCLUSION:①ELISA is specific and stable for GluR1 antibody in serum. ②There is the possible autoimmune response of GluR1 antibody in serum of epilepsy children.

Objective To study the association of the serum soluble intercellular adhesion molecule-1(SICAM-1) and soluble vascular cell adhesion molecule-1(SVCAM-1) with metabolic syndrome(MS) and its components. Methods The MS was defined by criteria of the international diabetes federation. The levels of SICAM-1 ,SVCAM-1,plasma glucose,insulin,lipids,uric acid, glycosylated haemoglobin A(1C), and blood viscosity were measured. Results The concentrations of SICAM-1 and SVCAM-1 were significantly higher in subjects with MS and its components than in those without them. The concentrations of SICAM-1 and SVCAM-1 were correlated positively with disorder number of BMI, WHR, SBP, DBP,FPG, 2 hour plasma glucose, triglyceride, uric acid, HbA1c, blood viscosity and HOMA-IR,and negatively with high density lipoprotein cholesterol. Conclusions The concentrations of SICAM-1 and SVCAM-1 rise with the increase of the number of MS components. Endothelial dysfunction might participate in the occurrence and development of MS.

Objective:To determine the CD 30 expression in patients with hemorrhagic fever with renal syndrome(HFRS).Methods:The double immunofluorescence technique and flow cytometry were used.Results:There is no significant difference among the three groups of HFRS patients on the CD + 4CD - 30 T lymphocyte subset.While the CD + 4CD + 30 T cells increase and the difference between severe group and mild-moderate group is very significant(P

Objective To investigate the effects of dieting and exercise intervention on lipid level in patients with hyperlipidemia.Methods Health education including dieting and exercise interventions was given to 1,800 patients with hyperlipidemia.Serum total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL-C)and low density lipoprotein (LDL-C)levels were assessed before and after interventions.Result TC, TG and LDL-C levels were decreased and HDL-C increased after interventions with statistical significance(P<0.01).Conclusions Dieting and exercise intervention can be effective in controlling lipid levels effectively.Health education should vary among individuals to achieve good effects.

Objective To investigate the difference of serum high sensitivity C-reactive protein(hs-CRP)concentrations in subjects with various glucose tolerances,and to analyze the correlation with insulin resistance.Methods A total of 90 subjects,who came from the Medical Examination Center of the First Hospital Affiliated to China Medical University from Oct.2004 to Apr.2005,were divided into five groups according to oral glucose tolerance test(OGTT):normal glucose tolerance(NGT)group;impaired fasting glucose(IFG)group;impaired glucose tolerance(IGT)group;IFG and IGT(IGT/IFG)group;newly diagnosed,complication-free type 2 diabetes(T2DM)group.By using the ELISA methods,the concentration of serum high sensitivity C-reactive protein(hs-CRP)was measured in these subjects,and its correlation with homeostasis model assessment insulin resistance index(HOMA-IR)was andlysed.Results The concentration of hs-CRP was significantly higher in IFG,IFG/IGT,T2DM than in NGT.The concentration of hs-CRP was positively correlated with glucose metabolism index and insulin resistance index.Conclusion Our study demonstrates that inflammation already exists not only in T2DM patients but also in subjects with IFG.Inflammation might participate in the occurance and development of T2DM.

To explore the role of mechanosensitive potassium channel TREK-1, Western blot analysis was used to investigate the expression changes of TREK-1 in left ventricle in acute mechanically stretched heart. Forty Wistar rats were randomly divided into 8 groups (n=5 in each group), subject to single Langendorff perfusion for 0, 30, 60, 120 min and acute mechanical stretch for 0, 30, 60, 120 min respectively. With Langendorff apparatus, an acute mechanically stretched heart model was established. There was no significant difference in the expression of TREK-1 among single Langendorff perfusion groups (P>0.05). As compared to non-stretched Langendorff-perfused heart, only the expression of TREK-1 in acute mechanically stretched heart (120 min) was greatly increased (P<0.05). This result suggested that some course of mechanical stretch could up-regulate the expression of TREK-1 in left ventricle. TREK-1 might play an important role in mechanoelectric feedback, so it could reduce the occurrence of arrhythmia that was induced by extra mechanical stretch.
Feedback ; Heart Ventricles/*metabolism ; Mechanotransduction, Cellular ; Potassium Channels, Tandem Pore Domain/*metabolism ; Random Allocation ; Rats, Wistar ; Stress, Mechanical

Objective To investigate the clinical and pathological diagnosis of cutaneous calcifying epithelioma.Methods Thirty cases of calcified epithelioma were diagnosed and treated by our hospital.The clinical features and pathological features were retrospectively reviewed.Results The main pathological features of the tumor were that the main tumor (basophilic cells) basal cells and shadow cells were mixed composition,combined with ossification and calcification and with no recurrence after surgical resection.Conclusion The clinical manifestations of calcifying epithelium are lack of characteristics and diverse pathological features.It is necessary to make the correct pathological diagnosis with the time,location and imaging of tumor growth.

Objective To investigate the effects of polyi:c on angiogenesis in mouse prostate carcinoma tissue and their possible mechanisms.Methods Mouse prostate carcinoma models were randomly divided into two groups according to tumor weight:control group and polyi:c group.After treatment for 7 times,the mice were sacrificed and the tumor tissues were cut for weighing,calculating the tumor inhibitory rate and tumor index.Hematoxylin-eosin staining and immunohistochemical staining were performed to observate the morphological changes of prostate carcinoma tissues,distribution of vasa and expressions of vascular endothelial growth factor (VEGF) and endothelial nitric-oxide synthase (eNOS).Results In polyi:c group,the mean tumor inhibitory rate was 67.85% and the tumor index was(5.42?0.17)%;in control group,the tumor index was(14.45?1.06)%; there was significant differences between polyi:c group and control group( P

Objective To prepare a high‐titer rabbit specific serum antibody against Kaposi′s sarcoma associated herpesvirus (KSHV) ORF65 capsid protein and identify the specificity of serum antibody .Methods Artificial synthetic peptide of ORF65 pro‐tein was emulsified with Freund adjuvant .4 rabbits were immunized with the prepared antigen by subcutaneous injection at various sites of skin of back and jaw once every two weeks .Immunization was carried out in total 4 times .The serum of the immunized rab‐bits was collected at a week after the last immunization .The titer of rabbit anti‐serum was assayed by ELISA .Specificity of the rab‐bit anti‐serum was analyzed by immunofluorescence assay and Western blot .Results The immunized rabbits produced high‐titer se‐rum antibody after total immunization .The highest titer of anti‐serum against ORF65 protein peptide was 1∶12 800 .The results of Immunofluorescence assay showed that antibody was binded in plasma of BCBL‐1 cell mostly ,which was consistent with the expres‐sion location of ORF65 in BCBL‐1 cell .Subsequently ,the data of Western blot revealed a specific band about 21 kD which accorded with the size of ORF65 protein .Meanwhile ,the expression of ORF65 in TPA treated BCBL‐1 cells was higher than the control cells ,which was consistent with the expression characteristics of lytic protein .Conclusion High‐titer specific rabbit serum antibody against KSHV capsid ORF65 antigen could be successfully prepared by rabbits immunization with ORF65 protein peptide .The pre‐pared antibody could be revealed immune reaction specificity with KSHV ORF65 protein .