Flow cytometric immunophenotyping has evolved from two-parameter quantitative measurement of peripheral blood lymphocytes to five-or more parameter qualitative evaluation of bone marrow and lymph node in hematopathology.Leukemia/lymphoma immunophenotyping represents an important addition to histomorphology in the diagnosis,classification and monitoring of hematolymphoid neoplasms.The complexity of five-or more parameter analyses and the interpretation of the data rely on standardization and validation of the instrument,the reagent and the procedure.In addition,clinical flow cytometry laboratories in U.S.A are required to document proficiency testing,sample preparation as well as accuracy,specificity,sensitivity and precision of methodology.CLSI and UCCC recommend that each laboratory should validate its own qualitative and quantitative procedures.This paper introduces the procedures for validation and quality control in a clinical flow cytometry laboratory in the United States.

Histone deacetylases (HDACs) catalyze the deacetylation of histone and nonhistone proteins. They play important roles in regulating chromatin remodeling and gene transcription,and that aberrant chromatin remodeling and transcriptional dysregulation are associated with many neurodegenerative diseases. Preclinical studies have suggested that histone deacetylases inhibitors (HDACIs) can target diverse pathophysiologies of ischemic stroke,not only reduce neuronal damage and infarct volume,but also promote the neuronal plasticity and functional recovery after ischemia. This article mainly reviews the neuroprotective mechanisms of HDACIs in ischemic stroke.

The current prevalence of myopia has been as high as 27.65%～53.80%among adolescents in Chinese schools.The major reasons are too much near-work activity and lack of outdoor activity for a long time.In order to reduce the prevalence of myopia in Chinese adolescents,we suggest that Chinese students should have 3.5 hours outdoor activity everyday,including 30 minutes travel between home and school,1 hour outdoor activity between classes,1 hour outdoor sport class at school,and 1 hour outdoor activity after school.

Objective To investigate the clinical effect and nursing methods of bilevel positive air-way pressure(BiPAP)ventilation in patients with neurogenic pulmonary edema(NPE).Methods Totally 11 NPE patients from January 2004 to December 2007 were enrolled.Ventilation support adopted specific invasive BiPAP mode of Puritan-Bennett840.Aiway pressure release ventilation(APRV)tactics was ap- plied in this mode.The blood oxygen saturation rote(SpO2),acidity-alkalinity(pH),arterial oxygen pres-sure(PaO2),arterial carbon dioxide pressure(PaCO2)were analyzed and compared between synchronized intemittent mandatory ventilation(SIMV)mede2 h and 24 h after BiPAP ventilation.Results Among,arterial blood gas analysis of 11 patients,SpO2 and PaO2 were obviously ameliorated after BiPAP ventilation compared with SIMV ventilation.No side-effect occurred.Conclusions The application of invasive Bi- PAP ventilation support in NPE patients proved to be a fast and effective method.

Objective: The pathology workflow statistics, modeling, optimize procedure and establish standardized operating procedures. Methods:This paper which based on the previous studies, the standard operation management and database technology, based on pathology workflow sample collection, sample inspection, warehousing storage, picking or sorting, sample processing and handling, inspection, quality control and other aspects of the analysis, optimizing operating procedures, thus forming SOPs, and accordingly the establishment of standardized management database system. Results: The system shortens the pathological examination cycle, improve the detection efficiency. Conclusion: It can be widely used in pathology sample collection, diagnosis, management processes, and application for sound management system pathology, pathology strengthen quality control is important.

Objective To study how ANGPTL4 modulates phenylephrine(PE)-induced cardiac fibrosis. Methods PE was applied to induce cardiac fibrosis. The knockdown effect of ANGPTL4 under PE stimuli was confirmed by Western blot;the proliferation of cardiac fibroblast was detected by CCK-8;mRNA levels of Collagen 1(Col1)and connective tissue growth factor(CTGF)were detected by QPCR to investigate the regula-tion of cardiac fibrosis by ANGPTL4. Results PE induced the proliferation of cardiac fibroblast and the up-regula-tion of mRNA levels of Col1 and CTGF;ANGPTL4 knockdown deteriorated cardiac fibrosis,manifested by the up-regulation of Col1 and CTGF. Conclusion ANGPTL4 inhibits PE-induced cardiac fibrosis.

Objective To investigate the Toxoplasma gondii infection status in pregnant women with history of adverse preg?nancy and risk factors in Bazhou area,Hebei Province. Methods A total of 302 pregnant women with the history of adverse pregnancy were chosen as respondents(an experimental group)in the hospital from March 2012 to December 2015,and 197 pregnant women without the history of adverse pregnancy as a control group. TOX?IgG and TOX?IgM were detected by using ELI?SA in two groups. The risk factors of Toxoplasma infection were surveyed by questionnaires. Results The total positive rate of Toxoplasma antibodies was 28.15%(85/302)in the experimental group,which was significantly higher than that[9.64%(19/197)]in the control group,and the difference was statistically significant(χ2=24.76,P<0.05). The positive rates of TOX?IgM,TOX?IgG and TOX?IgM+TOX?IgG were 6.95%(21/302),18.54%(56/302),and 2.65%(8/302)respectively in the ex?perimental group,which were higher than 2.03%(4/197),7.61%(15/197),and 0%(0/197)respectively in the control group (χ2=6.07,11.67,3.76,all P<0.05). The questionnaire survey showed that the proportions of keeping pets,cutting board re?gardless,liking to eat hot pot or barbecue,eating raw meat,often eating in the restaurant in the pregnant women with Toxoplas?ma infection were higher than those in the pregnant women without Toxoplasma infection,and the differences were statistically significant(χ2=22.57,3.96,5.87,7.40,4.86,all P<0.05),and therefore,the above unhealthy habits may be important risk factors. Conclusions Toxoplasma infection could lead to adverse pregnancy outcomes. Therefore,the above?mentioned unhealthy habits should be avoided,especially during pregnancy period.

Objective To observe the anal exhaust effect in patients treated with warm water foot bath after laparoscopic surgery.Methods 90 cases after laparoscopic surgery including renal cancer , renal cysts, epinephrine-tumor patients were randomly divided into A , B and C group.Nursing measures were given at 6 hours after operation. A group were treated with warm water feet bath ; B group received abdominal ring massage ; And C group were control group.Time of anal exhaust postoperatively was observed among the 3 groups.Results Anal exhaust time after oper-ation in A group was average (24.34 ±0.28) hours, which was significantly shorter than in B group (33.07 ±0.18) h and C group (37.90 ±0.19) h (p <0.01).Conclusion Warm water foot bath can promote the anal exsufflation rapidly, effectively after laparoscopic surgery in Urology patients , and has great significance helpful to early recovery .

Objective To investigate whether apoptosis is invovled in the immune response of inner ear,and relation between the signal transduction of Caspase-3 and apoptosis. Methods Sixteen healthy,female guinea pigs were employed in this study. The animals in the experiment group were sensitized systematically with keyhole limpet hemocyanin(KLH),then were inoculated in inner ear with the same antigen, and the animals in the control group were injected with the same volume of PBS. The animals were sacrificed at the 5th day after inner ear vaccination. Paraffin sections of cochleas from animals were prepared, TUNEL assay was used to detect apoptotic cells in inner ears, and immunohistochemical method was applied to detect the expression of Caspase-3 in inner ears. Results TUNEL-positive cells are found in the inner ears of the experiment group, but did not in the inner ears of the control group except for a few TUNEL-positive cells in the supporting cells, the stria vascular cells and the spiral ganglion cells. The high expression of Caspase-3 could be detected in the inner ears of the experiment group, but no positive cells of Caspase-3 were found in the inner ears of the control group. Conclusion Apoptosis could be induced by the immune response of inner ear, and Caspase-3 cascade may be involved in the course of apoptosis.

The relationship between medical practitioners and padtients always changes with time and this shift becomes quite striking nowadays.The characteristics and underlying reasons of this trend are expounded from such aspects as the changes in medical service system,the alteration in definitions of medical practitioners and patients, the advent of market economy.It is precondition and guarantee of handling the relationship properly to consummate relevant laws and codes,establish medical insurance institution,classify hospitals and set moral restraint for both medical practitioners and patients.