Objective] In order to guide the treatment based on syndrome differentiation and to provide theory and thinking method for further research , the article researched the experience in treating phlegm syndrome of Pro.Wu Weikang,a prominent physician of Longjiang medical school,and discussed the four treatments of phlegm syndrome. [ Methods] The article researched experience in treatment of phlegm syndrome mainly from four aspects: ①searching for the primary cause of disease in treatment; ②harmonizing method with warm medicine; ③treat qi before phlegm; ④synchronic treating phlegm and blood stasis. [Results] Pro.Wu Weikang maintained that strange and chronic diseases are accompanied with phlegm. Thus he advocated that searching for the primary cause of disease in treatment,the treatment with mild medicine,the priority over qi treatment and the simultaneous treatment of both phlegm and stasis. These principles are widely used in clinic and highly effective. [Conclusion]The experience in prescription component of the treatment of phlegm syndrome not only has a high scientific content ,but also has significant guidance of clinical practice.

Objective To explore the effect of swallowing training on dysphagia in patients with head and neck neoplasm receiving ra-diotherapy. Methods 60 patients with head and neck neoplasm receiving radiotherapy from March, 2014 to August, 2015 were divided into intervention group (n=30) and control group (n=30) randomly. The control group received routine nursing and relevant health education, while the intervention group received swallowing training from 2 weeks before to 3 months after radiotherapy. They were assessed with Functional Oral Intake Scale and M. D. Anderson Dysphagia Inventory 1 week, 3 months after radiotherapy. Results The scores of Function-al Oral Intake Scale (Z=-3.195, P<0.01), the total score of M. D. Anderson Dysphagia Inventory and the subscores (t>4.385, P<0.01) were better in the intervention group than in the control group 1 week after radiotherapy, as well as 3 months after radiotherapy (Z=-4.436, P<0.01;t>5.361, P<0.01). Conclusion Swallowing training could improve the condition of dysphagia of head and neck neoplasm patients after radiotherapy.

Objective To develop a new method for detection of anti-dsDNA antibodies Methods Plasma double stranded DNA were used as antigen substrate and derived with fluorescence thiazole orange homodimer Capillary electrophoresis with laser induced fluorescence was used for the detection of anti dsDNA antibodies of patient with Systemic lupus erythematosus (SLE) Results 23 out of 40 patients with SLE were positive(57 5%); 1 out of 25 patients with other autoimmune disease were positive; none of 45 controls were positive Comparison of this assay with Farr and dot immunobinding assay(DIBA)shows that CE LIF was more sensitive than DIBA and more specific than Farr Conclusions The assay was rapid, simple and reproducible CE LIF may become a routine assay for determination of anti dsDNA antibodies

[Objective] In order to guide the clinical syndrome differentiation and treatment, and provide the basis for further research on the ideological system of Wu Weikang, a famous professor of Longjiang medical school, the article studied Wu Weikang's experiences in the treatment of nephropathy and discussed the three basic prescriptions of him for treatment of kidney diseases. [Methods] The article discussed experiences in the treatment of nephropathy and academic thought on the basic of the three prescriptions of Wu Weikang such as eight-ingredients detoxification decoction ,yinqiao dihuang decoction and jiawei shenqi decoction. [Results] According to the common pathogenesis and kidney disease clinical symptoms, Professor Wu Weikang often chooses eight-ingredients detoxification decoction,yinqiao dihuang decoction and Jiawei Shenqi decoction as the basic prescriptions applied to the treatment of the dampness-heat of bladder syndrome, kidney deficiency and dampness-heat of bladder syndrome, and edema syndrome due to kidney yang deficiency, and the curative effect is remarkable. [Conclusion] Eight-ingredients detoxification decoction ,yinqiao dihuang decoction and Jiawei Shenqi decoction are the three prescriptions commonly used to treat the nephropathy by professor Wu Weikang in clinic. These prescriptions are widely used in clinic and highly effective. Practical experiences of these prescriptions will be elaborated here, so as to extract their essences to guide clinical practice.

Objective To establish the simultaneous determination of arsenic and mercury in the drinking water by hydride generation atomic fluorescence spectrometry (HGAFS). Methods The contents of trace arsenic and mercury in drinking water were detected by HGAFS based on optimized working conditions in this assay. The influences of some factors, such as sample pretreatment, pH value and interfering ions in drinking water were analyzed also. Results The detection limit of arsenic, mercury was 0.29 ng/ml and 0.05 ng/ml respectively. The precision (relative standard deviation) of the method was less than 6.8% .The recovery rates ranged from 85.7%～112.6%. The detecting results for standard materials were very much close to the reference values. Conclusion This method was suitable for the detection of arsenic and mercury in drinking water.

AIM:To find a new way of treating premature beats(PB) to evaluate the efficacy and safety of Yi-(xinshu) Capsule(Radix et Rhizoma Ginseng,Radix et Rhizoma Salviae miltiorrhizae,Radix Ophiopogonis,Radix Astragali,etc.) METHODS:Randomized,controlled clinical study were conducted in 60 patients with atrial premature beats(APB),and 60 patients with premature ventricular beats(PVB).APB patients were randomly divided into two groups:Yixinshu Capsule plus Isoptin(study group,n=30) and Isoptin group(control group,n=30).PVB patients:Yixinshu Capsule plus Mexiletine(study group,n=30) and Mexiletine group(control group,n=30).Those patients were treated for four weeks.We used 24 h Holter to monitor the times of PB and myocardial ischemia in before and after treatment.RESULTS:In the study group and control group,the times of APB and PVB were decreased obviously after 4 weeks treatment(P

This paper discusses the issues related to research capacity building in a military teaching hospital.We analyzed the opportunities,challenges and proposed development of military hospitals in order to strengthen the collaboration,,to adjust disciplinary structure,to consolidate research platform,to promote international exchanges,and to train the talented researchers and eventually to improve the hospital's overall research capacity.

Objective To analyze the chromosome karyotypes,prenatal diagnosis indications and pregnancy outcomes of high-risk pregnant women in Guangzhou.Methods 2 475 cases pregnant women with screening high risk were operated amniocen-tesis or cordocentesis from January 2010 to September 2012,then amniotic fluids and cord bloods were cultured and the cell were collected for chromosome preparation,G banding,karyotype analysis.We completed follow-up works lastly.Results 38 cases were detected chromosomal abnormality(including 12 cases Down′s syndrome,9 cases sex chromosome abnormality,7 cases transloca-tion,5 cases Edwards′syndrome,2 cases inversion,2 cases deletion,1 cases triploid),the abnormal rate was 1.54%.132 cases were detected chromosomal polymorphism(60 cases 1,9,16qh+ ,30 cases inv(9),25 cases D/Gs+ ,17 cases Y polymorphism).Research on prenatal diagnosis indications,there were 449 cases advanced age,668 cases Down′s screening with high risk,158 cases with ab-normal B ultrasound screening,38 cases with adverse pregnancy history.Conclusion The highest percentage abnormal karyotype is Down′s syndrome.Down′s screening high risk is the main reason for prenatal diagnosis.It is very important to do prenatal diagnos-tic and system B ultrasound for the high-risk pregnant women.

Objective To design and prepare an RNA interfering vector for effectively inhibiting the cellular expression of zinc finger protein A20 and observe the effect of A20 gene silence on cellular inflammatory response. Methods Specific RNA interfering oligonucleotide fragments (ASRF) were designed and synthesized artificially and the A20 RNA interfering vector pSUPER-EGFP-A20 siRNA constructed. Human monocyte cell line THP1 was used to infect the pSUPER-EGFP-A20 siRNA by means of genetic transfection technique; then, silence rate of cellular A20 was analyzed by real-time polymerase chain reaction (PCR). In the meantime, the activity of nuclear transcription factor nuclear factor-κB (NF-κB) and the level of tumor necrosis factor-α (TNF-α) in culture supernatant were measured by ELISA. Results Of two specific inhibitory oligonueleotide fragments of A20, the fragment M59465-385R/F had a higher inhibition to A20 expression, with rate of A20 gene silence of 83.86%. Preliminary application showed that after A20 gene silence, the activity of NF-κB was increased by 78.13% and the level of TNF-α in cell culture supernatant was increased by 49.30%. Conclusions Vector of A20gene silence with a high efficiency is obtained successfully. Preliminary application indicates that the expression of A20 can down-regulate the degree of cellular inflammatory responses.

Objective To investigate the effect of T lymphocyte proliferation,Th1 and Th2 cytokines on T cells from systemic lupus erythematosus (SLE)patients by blocking 4-1BB/4-1BBL.Methods The proliferation of T cells from 30 SLE patients and 20 normal controls was detected by MTT and the levels of patients stimulated with anti-CD3 McAb were significantly higher than those of T cells without stimulation(P＜IFN-γand IL-4 in SLE were significantly higher than those of normal controls(P＜0.01).There were more ex-pression of IFN-γ and IL-4 in supernatant of T cells from SLE after stimulated with anti-CD3 McAb(P＜0.01).However,the production of IFN-γ and IL-4 was inhibited by anti-4-1BB McAb(P＜O.05),and especially the level of IL-4 was markedly decreased.Conclusion Blocking 4-1BB/4-IBBL can significantlv inhibit the abnormal activation of T cells and the secretion of Thl and Th2 cytokines of SLE.