Complement-induced polymorphonuclearcytes (PMN) aggregate in microvasculature of vital organs, produce microemboli, and block microcirculation blood flow. Activated PMN release oxygen free radicals, prostaglandins and proteases, in turn, damage endothelial and parenchyma cells. This is an important factor in pathogenesis of multiple system organ failure. We have found that Sinomeuine and Sodium Ferulate prevented PMN aggregation induced by activated complement. Adding the drugs into PMN suspension treated with cytochalasin B, the zymosan activated plasma, PMN aggregation was prevented. The changes of light transmission were smaller than those of control without drugs recorded with platelet aggregometer. The 50% of PMN aggregation inhibition with Sinomeuine was 0.76mg/ml (1.gmmol/L), and 0.82mg/ml (3.8mmol/L) with Sodium Ferulate.

OBJECTIVE: To study the active fractions from Antipyretic analgesics powder.METHODS:The active fractions were extracted from Antipyretic analgesics powder by supercritical-CO2 fluid extraction technique,separated and purified by molecular distillation(MD) technique.The active fractions were selected with the analgesic effect as index.RESULTS: The active fractions obtained showed remarkable analgesic effect.CONCLUSION: The result serves as a theoretic basis for the development of the active fractions-based new drugs.

Objective To study the effect of noise on the ability of learning and memory of mice,and observe the effect of herba Houttuyniae injection on the change of the ability of learning and memory in noise condition, and then analyses the mechanisms. Methods To choose suitable mice and divide into groups, applying Y-maze and colorimeter to determine the content of MDA and the enzyme activity of SOD, AChE and NOS of mice brain. Results Learning group in Y-maze, noise cause the ability of noise group (12.1±1.20) decrease(P＜0.01) compared with the control group(15.1±1.45), but herba Houttuyniae injection can reverse the ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(14.9±1.60). The results is same in the memory group, in Y-maze, noise cause the memory ability of noise group (5.20±1.40) decrease(P＜0.01) compared with the control group(6.30±1.32), but herba Houttuyniae injection can reverse the memory ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(6.10±1.10). Either learning or memory group mice, noise can decreased the activity of SOD and NOS of mice brain(P＜0.01), but the content of MDA and the ability of AChE elevated(P＜0.01). herba Houttuyniae injection can change the effect of noise to mice, the activity of SOD and NOS of mice brain elevated(P＜0.01) and the content of MDA and the ability of AChE decreased (P＜0.01). Conclusion herba Houttuyniae injection can prevent the ability of learning and memory of mice which decreased by noise, and have the effect of intoxication.

OBJECTIVE:To study the incidence of adverse drug reactions(ADRs) and the rational use of Durogesic in in-patients METHODS:37 in-patients treated with Durogesic in Department of Oncology in our hospital were prospectively observed,and its analgesic efficacy and incidence,severity and the outcome of ADRs were evaluated RESULTS:The alleviating effect of Durogesic on carcinomatous pain was 100% Of 37 patients,ADRs were found in 4 cases with a ADR rate of 10 8% CONCLUSION:Durogesic is effective in clinical use,but attention should be paid to monitoring ADRs and avoiding irrational use of drug

BACKGROUND: Medical order-obeying behavior(MOOB) in patients with essential hypertension(EH) cau directly affect the progression and prognosis of the disease. What factors, in turn, affect MOOB?OBJECTIVE:To analyze factors affecting MOOB in EH patients.DESIGN: Cross-sectional study.SETTING: Foreign Guests Ward of Qindu Stomatological Hospital, Fourth Military Medical University of Chinese PLA.PARTICIPANTS: A total of 205 patients with EH were admitted into the Foreign Guests Ward of Qindu Stomatological Hospital, Fourth Military Medical University of Chinese PLA from January 1998 to August 2000. Inclusion criteria: In accordance with WHO diagnostic criteria for hypertension. Exclusion criteria: Diabetes and other cardiovascular diseases. Altogether 164 patients with the mean age of (51 ± 5) years, 122 men and 42 women , accorded to the inclusion criteria.METHODS: The 164 patients were investigated with the self-designed questionnaire concerning MOOB in EH patients; patients' sex, educational level, family economic conditions, disease duration and other factors affecting MOOB were observed.MAIN OUTCOME MEASURES:Factors related to the patients' MOOB.RESULTS:53.0% (87/164) patients followed the medical orders and persisted in exercise therapy, 58.5 % ( 96 / 164 ) patients followed reasonable diet, 51.6% (32/164)patients quitted smoking and alcohol drinking, 82. 3% (135/164) received regular recheck-up, 64.6% (106/164) followed the medicinal treatment prescribed. Sex did not influence MOOB significantly. The rate of medical-order disobedience was higher in illiterate people and those with education below junior middle school level, but lower in those with education above senior middle school level(x2 =7.25, P < 0.01) . The disobedience rate was lower in patients with the mean income of > 500 yuan/month than in those with < 500 yuan/month(x2 = 39.4, P < 0.01),and was also lower in patients with disease duration < 1 year than those with disease duration > 1 year (x2 =5.66, P < 0.05).CONCLUSION; Medical order-obeying behavior in EH patients is poor, and it is significantly influenced by patients' educational level, family economic conditions and disease duration.

OBJECTIVE To improve the knowledge of pulmonary aspergillosis. METHODS We analyzed the clinical features of 17 cases of pulmonary aspergillosis inpatients from May 1994 to Apr 2006 in our hospital retrospectively. RESULTS Sixteen cases were infected out of hospital,1 case was infected in hospital.The correct diagnostic rate of pulmonary aspergillosis was only 21.4% in the patients infected out of hospital,70.6% cases infected out of hospital were misdiagnosed to as tuberculosis or lung cancer. CONCLUSIONS The misdiagnosed reasons are that the some doctors are short of perceptual knowledge of aspergillosis and pulmonary aspergillosis confuses easily with TB and cancer and other underlying diseases.

Objective To evaluate the effect of multiple adhesion molecules ICAM-1、CD_ 44 V6、CD_ 28 、E-cad and CEA in organic selection of gastric carcinomatous metastasis.Methods Eighty-four gastric carcinoma cases(32 cases without metastases,52 cases with transfer to liver、marrow、lymphonode、lung、kidney or belly cavity),were take operation sample or tissue under gastroscopy,being made cells single by primary culture,then were detected the expression of 5 kinds of adhesion molecules by ABC immunohistochemistry,positive rate was recorded.Results ICAM-1 had high expression in cases with marrow or lung metastasis,while low expression in cases with lymphonode metastasis.CD_ 44 v6 had high expression in cases with lymphnode or liver metastasis.CD_ 28 had high expression in cases with liver、lung or kidney metastasis.E-cad had high expression in cases with marrow metastasis,while low expression in cases with other metastasis.CEA had especially high expression in cases with liver、belly cavity or lymphnode metastasis.Conclusion High or low expression of adhesion molecules ICAM-1、CD_ 44 V6、CD_ 28 、E-cad and CEA plays a certain role in organic selection of gastric carcinomatous metastasis.

Objective To evaluate the role of 4 types of adhesion molecules in digestive system tumors with bone marrow metastasis.Methods The fresh bone marrow slies of 72 cases of digestive system tumor with bone marrow metastasis,including 26 gastric cancer,25 liver cancer,21 colorectal cancer,were obtained.The tumor cell slides of 76 cases of digestive system tumor without metastasis,including 32 gastric cancer,20 liver cancer,24 colorectal cancer,were obtained.The expressions of 4 kinds of adhesion molecules in two groups were studied by ABC immunohistochemical methods,and positive rate was recorded and accumulated points.Results ICAM-1、E-cad and CEA positive rates/CD44V6 accumulated points were up-regulated in gastric cancer cells in bone marrow;ICAM-1、CD44V6 accumulated points/CEA positive rate were up-regulated in liver cancer cells in bone marrow,while expression of E-cad had no difference;ICAM-1、CD44V6 and CEA accumulated points/E-cad positive rate were up-regulated in large bowel cancer cells in bone marrow.Conclusion Different adhesion molecules play coordinative or resistant role in digestive system tumor with bone marrow metastasis.

Objective To evaluate the typical multidrug resistance in colorectal cancer chemotherapy with drugs including 5-Fu,MMC and cisplatin. Methods The expression of mdrl gene and GP170 in thirty colorectal cancer specimens were detected by reverse transcription polymerase chain reaction and immunohistochemistry.At the same time,the sensitivity of the cancer cells to six common chemotheraputic drugs were also detected by MTT colorimetric assay. Results In thirty colorectal cancer specimens,the expression of mdr_1 gene(63.3%) and GP170 (73.3%) were not different,and was concordant with the results of ADM cytotoxicity assay (P

OBJECTIVE:To explore the effect of nifedipine combined with losartan on the blood pressure and renal function of hypertension complicated with coronary heart disease. METHODS:150 patients with hypertension complicated with coronary heart disease were randomly divided into observation group and control group. Control group was orally treated with Nifedipine sustained release tablet 30 mg,once a day;observation group was additionally treated with Losartan potassium capsules 50 mg,once a day. The treatment course for both groups was 1 month. Systolic blood pressure,diastolic blood pressure,renal function indicators be-fore and after treatment,and incidence of adverse reactions in 2 groups were observed. RESULTS:After treatment,systolic blood pressure and diastolic blood pressure after 3 months was lower than 1 month and lower than before treatment in same group,renal function indicators were significantly lower than before treatment,and observation group was lower than control group,the differ-ences were statistically significant(P<0.05);the incidence of adverse reactions in observation group was significantly lower than control group,the difference was statistically significant(P<0.05). CONCLUSIONS:Nifedipine combined with losartan can well control the blood pressure of hypertension complicated with coronary heart disease,protect renal functions,with good safety.