Objectiv e To evaluate clinical application effect of the self-developed usableness and fully functional fuming-washing appliance used for limb rehabilitation.Methods 200 cases of patella fracture patients with knee joint stiffness were divided into the observation group and the control group with 100 cases in each group.The observation group used fuming-washing appliance for limb rehabilitation,the control group chose traditional clinical method.The clinical effect was compared between two groups.Results After 2 weeks,87 cases received excellent result,10 cases with good result,3 cases with fair result.The effective rate reached 97％ in the observation group,which was higher than that of the control group.Conclusions The fuming-washing appliance used for limb rehabilitation has definite clinical effect and good security,it can effectively promote the nursing quality and save rehabilitation nursing resources,meanwhile it can make use of the advantages and ef-fecfiveness of traditional Chinese medicine in the department of traumatology rehabilitation treatment.

Objective To study the effect of noise on the ability of learning and memory of mice,and observe the effect of herba Houttuyniae injection on the change of the ability of learning and memory in noise condition, and then analyses the mechanisms. Methods To choose suitable mice and divide into groups, applying Y-maze and colorimeter to determine the content of MDA and the enzyme activity of SOD, AChE and NOS of mice brain. Results Learning group in Y-maze, noise cause the ability of noise group (12.1±1.20) decrease(P＜0.01) compared with the control group(15.1±1.45), but herba Houttuyniae injection can reverse the ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(14.9±1.60). The results is same in the memory group, in Y-maze, noise cause the memory ability of noise group (5.20±1.40) decrease(P＜0.01) compared with the control group(6.30±1.32), but herba Houttuyniae injection can reverse the memory ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(6.10±1.10). Either learning or memory group mice, noise can decreased the activity of SOD and NOS of mice brain(P＜0.01), but the content of MDA and the ability of AChE elevated(P＜0.01). herba Houttuyniae injection can change the effect of noise to mice, the activity of SOD and NOS of mice brain elevated(P＜0.01) and the content of MDA and the ability of AChE decreased (P＜0.01). Conclusion herba Houttuyniae injection can prevent the ability of learning and memory of mice which decreased by noise, and have the effect of intoxication.

OBJECTIVE:To establish a microbial limit test method for povidone iodine solution.METHODS:Validation on methodology of the microbial limit test of povidone iodine solution was performed using plating method(Ⅰ),culture agent dilution method(Ⅱ),membrane-filter procedure method(Ⅲ)and sodium thiosulfate neutralization in combination with membrane-filter procedure method(Ⅳ).RESULTS:The recovery rates of all of the test organisms in method Ⅰ and method Ⅱ approached zero,less than 70% in method Ⅲ but above 85% in method Ⅳ.The growth of the control bacteria was abnormal in method Ⅱand method Ⅲ,but normal in method Ⅳ.CONCLUSION:It is advisable to adopt the method Ⅳ for microbial limit test of povidone iodine solution.

OBJECTIVE To investigate the phlegm etiology and the prevention measures of respiratory tract infection in 89 cases with heavy type hepatitis in hospital.METHODS From Jan 2004 to May 2007,the phlegm from respiratory tract hospital infection patients were studied retrospectively.RESULTS The main respiratory tract pathogens were the G-bacillui(44.5%),G+ cocci(23.2%),Candida albicans(16.8%),Aspergillus(9.0%) and yeast-like fungi(4.5%).CONCLUSIONS The investigation of the etiology suggests that the prevention of respiratory tract infection be the main measure to decrease hospital infection in heavy type hepatitis patients.

Objective To explore the effect of histamine in paraventricular nucleus (PVN) on the development of neurogenic pulmonary edema (NPE).Methods NPE models of rabbits were made and H 1 receptor antagonist (chlorpheniramine, H 1 group), H 2 receptor antagonist (Cimetidine, H 2 group) or normal sodium (NS group) were injected into PVN of rabbits utilizing the technique of stereotaxis. The degree of pulmonary edema was observed, the content of histamine in PVN was measured by high performance liquid chromatogram, and the ration of lung-water and A/P value were calculated.Results (1) Compared with control group and sham operation group, the content of histamine of PVN in NPE group obviously increased ( P

The perioperative nursing of sigmoid colon cystoplasty for neurogenic bladder patients including: preoperative psychological nursing, intestinal and vesical preparation; postoperative gastrointestinal decompression, drainage catheters and complications nursing; intermittent catheterization training before discharge; follow-up and healthy direction after discharge.

Electric and electronic products are required to pass through the certification on electrical safety performance before entering into the market in order to reduce electrical shock and electrical fire so as to protect the safety of people and property. The leakage current is the most important factor in testing the electrical safety performance and the test theory is based on the perception current effect and threshold. The traditional method testing the current threshold for perception only depends on the sensing of the human body and is affected by psychological factors. Some authors filter the effect of subjective sensation by using physiological and psychological statistical algorithm in recent years and the reliability and consistency of the experiment data are improved. We established an experiment system of testing the human hody's current threshold for perception based on EEG feature analysis, and obtained 967 groups of data. We used wavelet packet analysis to detect a wave from EEG, and used FFT to do spectral analysis on alpha wave before and after the current flew through the human body. The study has shown that about 97.72% alpha wave energy changes significantly when electrical stimulation occurs. It is well proved that when the EEG feature identification is applied to test the human body current threshold for perception, and meanwhile alpha wave energy change and human body sensing are used together to confirm if the current flowing through the human body reaches the perception threshold, the measurement of the human body current threshold for perception could be carried out objectively and accurately.
Algorithms ; Electric Stimulation ; Electroencephalography ; Humans ; Reproducibility of Results ; Sensory Thresholds ; physiology ; Wavelet Analysis

The positive rate of autoantibodies was 29.7% in 1617 diabetic patients. The positive rate of monoautoantibody was lower than that of combined assay with 3 autoantibodies. Combined assay of multiple autoantibodies may enhance the positivity of early diagnosis of type 1 diabetes mellitus. Glutamic acid decarboxylase antibody (GADA) may be a better screening test for predicting insulin dependency, the prediction of GADA combined with protein tyrosine phosphatase antibody may approximate to 100%, whereas islet cell antibody assay may be needed in the adolescent patients.

AIM: To establish the cell model of hippocampal neurons impaired by the supernatant of microglia conditioned medium (MCM) induced by LPS, and to study the mechanisms of neuron damage and the protection effect of curcumin. METHODS: The microglia were activated and stimulated by LPS at concentration of 5 μg/L. The supernatant of activated MCM was used to stimulate the hippocampal neurons. The morphology of the neurons was observed under light microscope and the survival rate was detected by MTT. The availability of respiration in the hippocampal neurons was determined by detecting the ratio of lactic acid/pyruvic acid, and the expression of 3 types of 1,4,5-inositol triphosphate (IP3R1, IP3R2, IP3R3) was detected by RT-PCR. RESULTS: The hippocampal neurons were damaged severely by the supernatant of activated MCM, and the morphology of the neurons changed significantly. Compared to control group, the ratio of lactic acid/pyruvic acid in MCM group was elevated and the expression levels of IP3R1 and IP3R3 in the hippocampal neurons were significantly increased. The ratio of lactic acid/pyruvic acid and the expression of IP3R1 in curcumin+MCM group were evidently lower than those in MCM group. However, no effect of curcumin on IP3R3 expression was observed, the changes of neural morphology was also ameliorated in curcumin+MCM group. CONCLUSION: Curcumin protects rat hippocampal neurons from damage induced by LPS-induced microglia condition medium.

To explore the role of mechanosensitive potassium channel TREK-1, Western blot analysis was used to investigate the expression changes of TREK-1 in left ventricle in acute mechanically stretched heart. Forty Wistar rats were randomly divided into 8 groups (n=5 in each group), subject to single Langendorff perfusion for 0, 30, 60, 120 min and acute mechanical stretch for 0, 30, 60, 120 min respectively. With Langendorff apparatus, an acute mechanically stretched heart model was established. There was no significant difference in the expression of TREK-1 among single Langendorff perfusion groups (P>0.05). As compared to non-stretched Langendorff-perfused heart, only the expression of TREK-1 in acute mechanically stretched heart (120 min) was greatly increased (P<0.05). This result suggested that some course of mechanical stretch could up-regulate the expression of TREK-1 in left ventricle. TREK-1 might play an important role in mechanoelectric feedback, so it could reduce the occurrence of arrhythmia that was induced by extra mechanical stretch.
Feedback ; Heart Ventricles/*metabolism ; Mechanotransduction, Cellular ; Potassium Channels, Tandem Pore Domain/*metabolism ; Random Allocation ; Rats, Wistar ; Stress, Mechanical