OBJECTIVE:To provide reference for clinical rational use and management of anti-HIV drugs,and to provide ref-erence basis for occupational exposure to develop free drug policy. METHODS:Using retrospective study method,according to DDD calculation method recommended by WHO,consumption sum,DDDs,DDC and B/A of anti-HIV drugs in our center during 2011-2014 were analyzed and evaluated. RESULTS:The consumption sum of anti-HIV drugs increased firstly and decreased later, increasing from 512112 yuan in 2011 to 650854 yuan in 2012,decreasing to 496473 yuan in 2014. DDDs of anti-HIV drugs in our center during 2011-2014 decreased from 35932 in 2011,to 16363 in 2014,showing decreasing tendency. The consumption sum of nucleotide reverse transcriptase inhibitors during 2012-2014 took up the first place,and DDDs of nucleotide reverse tran-scriptase inhibitors took up the first place in 2011,2013,2014. DDDs of Zidovudine tablets took up the first place in 2013,2014, and that of Tangcao tablets took up top 3 places during 2011-2014. DDC of Tenofour disoproxil fumarate tablets,Zidovudine and la-mivudine tablets,Emtricitabine and tenofovir tablets,Lopinavir and ritonavir tablets were in high level,being more than 46 yuan. DDC of Zidovudine tablets,Stavadine tablets and Nevirapine tablets were in low level,being lower than 6 yuan. B/A of Tenofovir disoproxil fumarate tablets,Stavudine tablets and Efavirenz tablets ranged 0.5-1.5 in 4 years and B/A of Tangcao tablets was equal to 1,showing good synchronism of social and economical benefits. CONCLUSIONS:Clinical application of anti-HIV drugs in our center is rational basically,and the use of drug is basically consistent with the occupational exposure prevention. The use of TCM preparation is basically in line with the requirements of drug use in the clinic.

ObjectiveTo observe the clinical efficacy of acupoint application plus modified Yu Ping Feng decoction in treating cold due to weak constitution.MethodNinety-six patients with cold due to weak constitution were divided into two groups. The control group was intervened by regular Western medicine, while the treatment group was by acupointapplication plus modified Yu Ping Feng decoction.ResultThe total effective rate was 91.7% (44/48) in the treatment group, significantly higher than 79.2%(38/48) in the control group. A one-year follow-up study found that the disease duration and relapse frequency in the treatment group were markedly shorter and less than that in the control group (P<0.05).ConclusionAcupoint application plus modified Yu Ping Feng decoction can produce a significant efficacy in treating cold due to weak constitution.

Pediatric cardiac injury is common in primary heart disease and secondary systemic diseases.The pathophysiological processes include ischemic injury or direct injury, as well as focal or diffuse myocardial inflammatory lesions.Myocardial injury markers, including myoglobin, creatine kinase isoenzyme muscle B and troponin, are first used for the diagnosis of acute coronary syndrome(ACS) in adult, and have greatly improved the efficiency of diagnosis with their sensitivity and specificity.However, due to the very different pathogenesis of myocardial injury in pediatric and adult ACS, as well as the particularity that children are in continuous growth and development, it is necessary to evaluate the value of the myocardial injury markers and their biological reference intervals in pediatrics.

Objective To explore the effect of laparoscopic ovarian drilling technique in the treatment of polycystic ovarian syndrome.Methods 60 patients with polycystic ovarian syndrome were randomly devided into observation group( 30 cases) and the control group(30 cases).The effect of the two groups was observed.Results LH and T of all the patients were significantly improved after the treatments( t =27.2158,20.4752,9.7169,0.4551,all P ＜0.05);The ovulation rate and the pregnancy rate of observation group(86.7％ and 86.7％ ) was significantly higher than those of the control group( x2 =5.4545,9.3496,all P ＜ 0.05 ),and the OHSS rate of observation group was 0,significantly lower than that of the control group ( 16.7％ ) ( x2 =5.4545,P ＜ 0.05 ).Conclusion Laparoscopic ovarian drilling technique was effective and safe in treating polycystic ovarian syndrome.

[Objective] Via an empirical survey, this study is aimed to explore the imparting and mastering of Chinese cultural knowledge in China ’s present col ege English teaching, so as to offer some effective strategies for dealing with“Chinese culture aphasia”in current col ege English teaching in China. [Methods] Resorting to the research methodology of tests, questionnaires and interviews, it carries out an investigation of a number of col ege students and English teachers in Zhejiang Province into their mastering of Chinese cultural knowledge and its conveying in English as wel as the curriculum design of their col eges or departments. The relevant data derived from the research are analyzed qualitatively and quantitatively. [Results] The subjects al demonstrate the phenomenon of“Chinese culture aphasia”concerning both its comprehension and its English translation to varying degrees. Besides, cross-cultural commu-nication is twofold, so it is indispensible for learners to study simultaneously their native culture,which is a solid cornerstone and the crucial foundation for foreign language learning, but has been seriously deficient in col eges’curriculum design and students’textbooks; integrating Chinese as one of the com-pulsory courses and establishing corresponding examining and evaluating systems incorporating formative assessment and summative assessment, are sure to cope with“Chinese culture aphasia”in present col ege English teaching in China effectively. [Conclusion] The research and solution above are expected to shed light on teaching syl abus formulations and curriculum designs, enhance students ’humanistic quality and their communication capabilities in reality, and consequently popularize the excellent Chinese culture.

Objective To observe the effects and toxicity of paclitaxel liposome combined with cisplatin in the treatment of ad-vanced non-small cell lung cancer .Methods 80 cases of patients of advanced non-small cell lung cancer were randomly divided into study group and control group ,40 cases in each group .Study group was treated with paclitaxel liposome (135 mg/m2 ,IV drip 3 h , first days) ,control group received paclitaxel(135 mg/m2 ,IV drip 3 h ,first days) ,the two groups combined with cisplatin 75 mg/m2 ,IV drip ,for 3 days ,take 21 days as one cycle .Results The study group CR 0 .0% (0/40) ,PR 27 .5% (11/40) ,RR 27 .5% (11/40);control group CR 0 .0% (0/40) ,PR 25 .0% (10/40) ,PR 25 .0% (10/40) ,(P>0 .05) .The research group of rash ,muscle and joint pain ,peripheral neuropathy was significantly lower than that of the control group (P<0 .05) .The research group of progres-sion-free survival was 5 .3 months(95% CI 3 .713 -6 .408) ,the median survival period was 9 .3 months(95% CI 8 .337 -9 .907);control group of progression-free survival was 4 .6 months(95% CI 3 .187 -5 .547) ,the median survival period was 9 .4 months (95% CI 7 .787-11 .602) ,the two groups had no statistical significance (P>0 .05) .Conclusion Paclitaxel liposome combined with cisplatin in treatment of advanced non-small cell lung cancer clinical efficacy and long-term efficacy of taxol and quite ,but with less allergic reaction .

Objective To explore the relationship between serum reproductive hormone levels and premenopausal and postmenopausal breast cancer patients.Methods We recruited healthy women and breast cancer patients who came to our hospital from September 2007 to February 2012 for routine physical examination or surgery and divided them into premenopausal healthy group Ⅰ and breast cancer group Ⅰ ;Postmenopausal healthy group Ⅱ and breast cancer group Ⅱ,with 175 cases in each group.Reproductive hormone levels was measured in all cases.Results There were significant differences among the four groups on estradiol (E2),testosterone (T),progesterone (P),luteotropic hormone (LH),follicle-stimulating hormone (FSH),and prolactin (PRL) (F =76.459,57.224,82.932,161.047,74.801 and 15.246 respectively,P ＜ 0.05).In premenopausal cases:There were significant statistical difference on the levels of E2 [(61.5 ±32.2) ng/L vs.(74.1 ±41.6) ng/L],T [(48.1 ±22.2) μg/L vs.(80.1 ±41.8) μg/L],P [(2.9±1.6) μg/Lvs.(3.5 ±1.3) μg/L],LH [(1.3 ±0.9) U/L vs.(3.5 ±1.4) U/L],FSH [(14.8 ±8.9) U/L vs.(25.1 ±23.3) U/L],PRL [(15.8 ±6.7) μg/L vs.(39.4 ±27.4) μg/L] between the healthy Ⅰ group and the breast cancer Ⅰ group (P ＜ 0.05).In postmenopausal cases:There were significant statistical difference on the E2 [(18.8 ± 8.3) ng/L vs.(55.9±34.1) ng/L],T [(34.1 ±16.2) μg/L vs.(84.7 ±66.4) μg/L],P [(1.3 ±0.9) μg/L vs.(3.5 ±1.4) μg/L],LH [(38.1 ±33.7) U/L vs.(45.6 ± 31.2) U/L] in healthy group Ⅱand the breast cancer Ⅱ group (P ＜ 0.05).The difference was statistically significant on the E2 [(74.1 ± 41.6) ng/L vs.(55.9±34.1) ng/L],LH [(3.5 ±1.4) U/L vs.(45.6 ±31.2) U/L],FSH [(25.1 ±23.3) U/L vs.(70.5±58.2) U/L],PRL [(39.4 ±27.4) μg/L vs.(15.9 ±15.5) μg/L] between the breast group of premenopausal and postmenopausal (P ＜ 0.05).Conclusion Reproductive hormone levels has important clinical significance for the onset and development of breast cancer and provides a reference point for the treatment of menopausal and post-menopausal breast cancer.

Objective To study the relationship between interleukin(IL)-4,IL-12 and IL-18 and intrauterine infection of hepatitis B virus (HBV),in order to provide the basis for prevention and treatment.Methods Forty pregnant women with positive HBsAg and intrauterine infection of HBV were selected as study group,and 80 pregnant women with positive HBsAg and non-intrauterine infection of HBV were selected as control group.The levels of IL-4,IL-12,IL-18 were compared.Results The level of IL-4 was (51.56 ± 7.86) ng/L in study group,which was higher than that in control group [(31.67 ± 8.64) ng/L],and there was significant difference between two groups (P ＜0.01).The leveh ofIL-12 and IL-18 were (803.45 ± 132.64),(627.87 ± 134.38) ng/L in study group,which werelower than that in control group [(904.87 ± 231.86),(734.78 ± 165.67) ng/L],and there were significant differences between two groups (P ＜ 0.01).Conclusions The levels of IL-4,IL-12,IL-18 are correlated with intrauterine infection of HBV.The increase of IL-4 and the decrease of IL-18,IL-12 may lead to positive HBsAg pregnant women happening intrauterine HBV infection.

Objective To explore of integrated traditional Chinese and Western medicine treatment for peri -menopausal dysfunctional uterine bleeding ( bleeding ) .Methods According to the digital table ,was ginen to 66 pa-tients with perimenopausal dysfunctional uterine bleeding were randomly divided into the two groups .The control group uses the conventional western medicine treatment ,the treatment group was treated by combined Western and Chinese medicine .Before and after treatment ,the endometrial thickness ,hemoglobin value of the two groups was recorded ,and the clinical efficacy of the two groups was evaluated .Results The treatment group in the treatment of uterine endom-etrial thickness,hemoglobin value and clinical effective rate was (6.69 ±1.96) mm,(97.04 ±15.48) g/L,97%, which was better than those of the control group [(8.09 ±2.44) mm,(87.13 ±20.83) g/L,85%] (t =2.575, 2.129,χ2 =9.452,all P<0.05).Conclusion The joint Chinese and Western medicine treatment for Peri-menopa-usal dysfunctional uterine bleeding and it is worthy of promotion .

Objective To analyze the changes of bone mineral density (BMD) and glucose metabolism in patients with type 2 diabetes mellitus (T2DM) complicated with osteoporosis.Methods Eighty-six patients with T2DM were selected.The patients were divided into T2DM complicated with osteoporosis group and simple T2DM group according to the BMD value.The fasting blood glucose (FBG),fasting insulin (FINS),glycosylated hemoglobin (HbA1c) and course of disease were compared between the 2 groups,and the correlation was analyzed between the indexes and BMD.Ninety-three health examination people were selected as health control group.Results The rate of osteoporosis in health examination people was significantly lower than that in T2DM patients [28.0％(26/93) vs.59.3％(51/86)],there was statistical difference (P ＜ 0.05).There were statistical differences in HbA1c,FINS,BMD,course of disease between T2DM complicated with osteoporosis group (51 cases) and simple T2DM group (35 cases) [(8.57 ± 2.59)％vs.(7.26 ±1.68)％,(13.21 ±4.73) μU/L vs.(17.54 ±5.91) μU/L,(0.764 ±0.130) g/cm2 vs.(0.993 ± 0.123) g/cm2,(11.4 ± 6.4) years vs.(8.6 ± 4.7) years,P ＜ 0.05].The BMD in patients with T2DM was negatively correlated with course of disease,HbA1c,FINS (r =-0.306,-0.185,-0.269; P ＜ 0.05 or ＜0.01),there was no correlation between BMD with FBG (P ＞ 0.05).Conclusion The poor long-term blood glucose control,low insulin level,long course of disease are the risk factors for T2DM complicated with osteoporosis.