Exercise has been proposed as one strategy for improving or maintaining the structural competence of bone. In contrast with previous densitometric analysis using dual energy x-ray absorptiometry (DXA) which reported that areal bone mineral density (aBMD ; bone mineral content per projected area) increased with exercise, the studies using peripheral quantitative computed tomography (pQCT) revealed that an improvement in the mechanical properties of adult bone in response to exercise is related to negative changes in volumetric BMD (vBMD ; bone mineral content in a unit volume). Therefore, if exercise does not increase vBMD, what does it change? The pQCT studies showed that periosteal area and cortical area were significantly greater in trained bone, together with an increase in cortical thickness. Furthermore, by assessing geometric bone adaptation to mechanical loading, cortical thickness and the mechanical properties of trained and sedentary bone were compared along 64 directions centering the center of gravity of the bone on cross-sectional pQCT images. The differences in these parameters for both groups depended on the direction of measurement, suggesting that site-specific adaptation of bone to exercise is related to the geographical relation of bone to muscle. Thus, the improvement in the mechanical properties of bone in response to long-term physical exercise is related to geometric adaptation and not vBMD.

Objective　To obtain the normal ranges of different parameters in static posturography.Methods　Different parameters' values of 117 normal persons (10～19 years old) were detected by static posturography while they were in opening and closing eyes situations. Results　The mean values of different parameters in opening eyes situation are significantly lower that in closing eyes situation(P＜0.01). The values from the male and the female demonstrate no significant difference, values from 10～19 years old group and 70～79 years old group are significantly higher than from 20～69 years old group(P＜0.01). Conclusion　The static posturography is a perfect measure to analyze and evaluate the balance ability of human body in exact quantification, and could be one of the routine clinic tests, supplying quantificational, intellective and facile method for diagnosis and evaluation of dizziness and balance ability.

Objective To analyze the application of root angioplasty in the crown lengthening surgery. Methods A total of 80 patients that corresponding to criteria from January 2013 to December 2015 in repair outpatients of our hospital were selected and received retrospec-tive study,and they were randomly divided into the observation group and the control group according to different surgical methods with 40 ca-ses in each group. The observation group was given root angioplasty and crown lengthening surgery,while the control group was only given crown lengthening surgery. Check-up was performed at 6 weeks postoperatively and 3 weeks after restoration,the condition of root surface cov-ered by the gum,the condition of gingival recession of the restoration and the subjective satisfaction of the patients were observed. Results The average coverage of the observation group was 91. 3%,which was significantly more than 55. 3% of the control group (P<0. 05). The a-mount of the gum covering of the observation group was (3.1 ±1.3)mm,which was significantly larger than (1.9 ±1.0)mm of the control group(P<0. 05). Conclusion Root angioplasty can guarantee blood supply,reduce the damage,but the indication range is small,and it is influenced by many factors,and needs to incorporate the ideas of all sides when used in clinic.

Aim To observe the influence of emodin on bleomycin-induced pulmonary fibrosis in rats, and explore its protective mechanisms. Methods Sixty SD rats were randomly divided into normal control group, sham operation group, model group, low-dose inter-vention group, high-dose intervention group and pred-nisone group. Each group included 10 animals. Rats in the latter 4 groups were intratracheally administered with bleomycin to establish pulmonary fibrosis model. From the second day, rats in low-and high-dose inter-vention groups were intragastrically treated with 2 mL of 20 and 80 mg · kg-1 emodin, respectively. Predni-sone group were intragastrically administrated with 2 mL of 5 mg·kg-1 prednisone acetate. However, con-trol and model groups were treated with 2 mL of normal saline. All rats were sacrificed on day 28. Pulmonary tissues were then removed, and HE and Masson stai-ning were performed. The contents of hydroxyproline ( HYP ) , malondialdehyde ( MDA ) , superoxide dis-mutase ( SOD) , glutathione-peroxidase ( GSH-Px) and catalase (CAT) in pulmonary tissues were measured. Serum concentrations of tumor necrosis factor-α ( TNF-α) , interleukin ( IL )-6 and IL-17 were detected by enzyme linked immunosorbent assay ( ELISA ) . The expression of Kelch-like ECH-associated protein 1 ( Keap 1 ) , nuclear factor-erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) p65 in pulmo-nary tissues was analyzed using Western blot. Results The pulmonary inflammation and fibrosis in low-and high-dose intervention as well as prednisone groups was significantly improved when compared with model group ( P<0. 05 , 0. 01 ) . In comparison with normal control group or sham operation group, pulmonary HYP and MDA contents, Nrf2 and NF-κB p65 expression levels in the nucleus, and serum concentrations of TNF-α, IL-6 and IL-17 were increased ( P <0. 01 ) , but pulmonary SOD, GSH-Px and CAT contents and Keap 1 expression levels in the cytoplasm were de-creased ( P <0. 01 ) in model group. Upon treatment with low-and high-dose emodin or prednisone, pulmo-nary HYP and MDA contents, Keap 1 expression levels in the cytoplasm, NF-κB p65 expression levels in the nucleus , and serum concentrations of TNF-α, IL-6 and IL-17 were reduced while pulmonary SOD, GSH-Px and CAT contents and Nrf2 expression levels in the nu-cleus were enhanced as compared to model group ( P<0. 01 ) . The above indicators were significantly im-proved in high-dose intervention and prednisone groups compared with low-dose intervention group ( P <0. 05). Nevertheless, There was no significant differ-ence between high-dose intervention group and predni-sone group ( P >0. 05 ) . Conclusion Emodin may protect against rats with pulmonary fibrosis by enhan-cing antioxidative ability and inhibiting inflammatory response.

Objective To observe the expression of tissue transglutaminase (tTG) in renal tissues of diabetic nephropathy rats, and to investigate its contribution to the progression of diabetic nephropathy. Methods Streptozotocin-induced diabetic rats were sacrificed at 30 d, 60 d, 90 d, and 120 d respectively. Albuminuria excretion rate (AER), kidney weight index and serum creatinine (Scr) of the rats were measured. Hematoxylin and eosin (HE) staining and periodic acid-silver-methenamine (PASM) staining were used to observe the renal pathological changes. CollagenⅣ(ColⅣ) and soluble tTG protein in kidney were examined by immunohistochemistry,insoluble tTG by immunofluorescence. Expression of total tTG mRNA in kidney was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results AER, kidney weight index and Scr of diabetic rats were all progressive during the period of the experiment. Compared with control group, the levels of ColⅣ, soluble tTG and insoluble tTG protein in kidneys of hyperglycemic rats were significantly increased at 30 d, 60 d, 90 d and 120 d(P

Objective To evaluate fluid distribution in patients on maintenance hemodialysis (HD) by bioimpedance analysis and on the effect of adjusting the dry weight in hemodialysis patients.Methods Forty maintenance HD patients from the dialysis center of the Second Affiliated Hospital of Dalian Medical University were enrolled as study group.One hundred and two individuals who were tested of physical examination in the same hospital were enrolled as the control group.Sex and age of the two groups were recorded.Body weight,body high,blood pressure,bioimpedance of HD patients (pre-dialysis and post-dialysis) and controls were measured.Bioimpedance was measured by multifrequency segmental bioimpedance analysis,including right arm (RA) bioimpedance,trunk (TR) bioimpedance and right leg (RL) bioimpedance.Bioimpedance ratio (BIR) of three parts was calculated as of 100kHz and 20kHz including RA-BIR,TR-BIR and RL-BIR.Then eight HD patients who had high RA-BIA or TR-BIA according to the reference range which were obtained from 102 controls were chosen for dry weight adjustment.Post-dialysis body weight,blood pressure,and bioimpedance of the eight HD patients were measured again after adjusting the dry weight.Results (1) BIR of three parts in pre-dialysis HD patients were all significantly higher than that in the control group (P ＜ 0.05).BIR of three parts of the post-dialysis HD patients were still higher than that of the control group,but RL-BIR was not significantly (P ＞ 0.05).BIR of three parts of the post-dialysis HD patients were lower than BIR of three parts of the pre-dialysis HD patients,and there was significant different (P ＜ 0.05) with RA-BIR and RL-BIR.(2) After adjusting the dry weight,BIR of three parts of the post-dialysis HID patients were still higher than that of the control group,but none of them was significantly (P ＞0.05).BIR of three parts of the HD patients after adjusting the dry weight were lower than BIR of three parts of the HD patients before adjusting the dry weight,but there was no significant different with TR-BIR(P ＞ 0.05).After adjusting the dry weight,systolic blood pressure of the post-dialysis HD patients were significantly decrease[(150.00 ± 29.28) vs (140.63± 20.78) mm Hg,P＜ 0.05].Conclusions Bioimpedance analysis may be an effective method for adjusting dry weight in hemodialysis patients,and the bioimpedance of arms is the most effective method.The bioimpedance reference range of hemodialysis patients can be according to the reference range of normal individuals.

Objective To understand the pollution of fungi in natural mineral water sources for drinking. Methods Sampling was carried out in 73 natural mineral water sources supplying water for 69 manufactories of bottled mineral water for drinking. Results 982 strains of fungi were found in 45 water samples (61.64%) of the total 73 water samples. Fungi imperfect! revealed the highest detected rates. Phycomycetea, Ascomycetes, Basidiomycetes were all detected, but less frequently. Among 18 detected fungal genera, Aspergillus and Cladosporium were all the dominant genera, as well as Penicilliurn, Trichoderma and Fusarium were commonly detectable genera. No correlations were observed between the detected rates of fungus and total count of bacteria, total coliform, the concentrations of nitrite in source water. Conclusion The extragenous fungal contamination in the process of post-extraction might be the main factor resulting in the pollution of fungi in natural mineral water source.

Objective To explore the information distribution of PM2.5 periodical paper both in English and Chinese in China.Methods Comprehensive analysis result was made based on the overall output of literature,academic attention trend,literature distribution and growth trend,authority institution and author ranking using high volume database,data mining,clustering analysis and visualization technology.Results There has been a rapid rise in the Chinese PM2.5 periodical paper in recent 5 years,with a sharp rise during the year 2011 to 2013 and the biggest rise in 2012.The periodicals that carry Chinese PM2.5 literature are mainly in the subjects of environment,health,resources.Researching organizations are mainly domestic famous comprehensive universities or research institutions.Core journal lists include those of China science and technology information institute,Peking University,and Chinese academy of science.Research terms mainly include PM2.5 air pollution,haze,particulate matter.Compared to the PM2.5 literature in Chinese,there has been slower development of literature in English in recent 5 years.Research organizations mainly include famous comprehensive universities or research institutions,and most of the literature are embodied in SCI,EI and Medline.Conclusions Some certain achievements have been made towards the PM2.5 research in China and the overall academic level of periodical paper is high,laying solid foundation for having a scientific knowledge of PM2.5 and taking countermeasure for haze.

Objective Based on the previous research that the ethanolic extract from traditional Chinese medicine fructus forsythiae (Lianqiao) can obviously inhibit cancer cells in vitro, the article aimed to investigate the anti-proliferation effects of dammar-24-ene-3β-acetate-20S-ol (DM) extracted from fructus forsythiae on gastric cancer cells and its mechanism.Methods MTT assay was used to assess the anti-proliferation effects of DM on gastric cancer cells including SGC-7901, BGC-823, and MKN-45 in vitro.There were MKN-45 control group and its low dose and high dose groups, BGC-823 control group and its low dose and high dose groups, SGC-7901 control group and its low dose and high dose groups in the experiment.Flow cytometry was used to analyze the cell apoptosis rate.Cellquest software was used to analyze the results and record the ratio of cells at different cycles.DCFH-DA probe was applied to detect the ROS levels of blank control group, docetaxol group and DM group.The reaction system of microtubule assembly test was set with 10?mol/L docetaxol, 50 or 100 μmol/L DM final density and no medicine in blank control group.The readings of UV spectrophotometer were recorded.Microtubule assembly assay and microtubule immunofluorescence staining were applied to investigate the effects of DM on microtubule system.Results The inhibition ratio of 50 μg/L DM on the proliferation three gastric cell lines were all above 80%, with IC50s of MKN-45 11.72±1.35 μg/mL, BGC-823 17.19±0.82 μg/mL, SGC-7901 7.55±0.79 μg/mL.8 days′ low density culturing at 48 hours after 2 μg/mL DM treatment, compared with control group, the number of cell clones significantly reduced without much change in clone size, while 48 hours after 10 μg/mL DM treatment, besides a few clones of BGC-823, there were just several megascopic clones of SGC-7901 and MKN-45.In comparison with apoptotic cell ratio in MKN-45 control group[(21.1±2.5)%], its low dose group and high dose group resulted in significant rise of apoptotic cell ratio[(25.1±1.3)% and (55.2±2.3)%] (P<0.01).Compared with apoptotic cell ratio in BGC-823 control group[(13.2±2.5)%], its low dose group and high dose group resulted in significant rise of apoptotic cell ratio[(18.2±2.1)% and (41.8±2.1)%] (P<0.01).Compared with apoptotic cell ratio in SGC-7901 control group[(10.5±1.8)%], significant rise was observed in its high dose group[(41.8±2.1)%] (P<0.05) while no statistical significance was found in its low dose group[(12.3±1.6)%] (P>0.05).In comparison with MKN-45 control group, the ratio of cells at S phase decreased in its low dose group[(14.5±2.7)% vs (12.3±3.3)%,P>0.05].In comparison with BGC-823 control group, the ratio of cells at S phase increased in its low dose group[(12.2±5.4)% vs (20.2±2.1)%,P<0.05].In comparison with SGC-7901 control group, the ratio of cells at S phase increased in its low dose group[(21.5±3.8)% vs (31.3±2.6)%,P<0.05].From the detection of intracellular active oxygen after DM treatment, dose-dependent ROS level increased in all three cell lines 48 hours after 10μg/mL and 50μg/mL DM treatment.From the results of microtubule immunofluorescence staining, 48 hours after the treatment of IC50 docetaxol and 10μg/mL DM, the fluorescence signals were in local concentration and disorder.Conclusion Dammar-24-ene-3β-acetate-20S-ol demonstrated anti-proliferation effects due to the apoptosis induced by cell cycle arrest at S phase.

Objective To investigate the data of outpatient children,survey on the clinical characteristics of vertigo and provide baseline information for clinical diagnosis and treatment.Methods The questionnaires and clinical tests data of 553 children with vertigo were retrospectively analyzed.Results All the children and their parents received interviews,otological examinations and clinical audiological tests. Their ages were between 4 and 15,with the average at 9.51?2.83. ①The males were 341(61.66%) and female 212 (38.34%),with the gender ratio of male to female as 1.61:1. ②The peak of the age curves of treatment and the first onset of vertigo was 9.51 and 8.62-year-old,respectively. ③20.98% of children with vertigo had normal results with peripheral vertigo more common in the rest.④The semicircular canals were more likely to dysfunction if their relatives had vertigo or car sickness.Conclusion The age of children mostly with vertigo is between 6 and 11 years with more males than females. Vertigo itself can be a symptom but on the other hand,some show no positive signs even with vertigo.