Objective:To examine the effect of acid etching time on the bonding interface of non-carious cervical sclerotic dentin. Methods: Twenty extracted premolars with non-carious cervical lesions were randomly divided into two groups, the lesion surface was conditioned with Scotchbond Multi-Purpose Plus dentin bonding system. The etching time was 15 s and 30 s respectively. The bonding interface ultra-structures were compared with SEM. Results:In sclerotic dentin (15 s) , the hybrid layer was visible, with minimal resin tags in the dentinal tubules and, when presented, they were shorter. Doubling the etching time (30s) resulted in more resin tags with an hybrid layer formation on peritubular dentin. Conclusion: Doubling the etching time improved the ultrastructure of sclerotic dentin-resin bonding interface, and could be an efficient way to improve the bonding effect.

Objective To investigate the association of occupational stress,social support,type D personality and physical health of nurses.Methods Cross-sectional study design was adopted.We used cluster sampling to investigate 1 287 nurses serving in one tertiary hospital and two second-class hospitals in Guangzhou.The questionnaire was anonymous answered.The content of questionnaire included the demographic data,occupational stress of nurses,social support,type D personality and physical health,etc.SPSS 13.0 was used for statistical analysis,including descriptive analysis,Pearson correlation analysis and path analysis.Results 1 192 of questionnaires were effective.The occupational stress was negatively related with the physical health status and social supports,and social support was positively related with the physical health status.The type D personality was negatively related with social supports and the physical health status,while positively related with occupational stress.Conclusions Occupational stress can make directly negative effect on the physical health.Social support has a directly protective effect on physical health.Type D personality makes direct and indirect negative effect on the physical health of nurses.

Objective To analyze the telephone follow-up response rate and related factors in lung cancer patients thus to explore the countermeasures to increase the telephone response rate.Methods One thousand six hundred and eight post-surgery lung cancer patients were followed up by telephone interview,calculate response rates and analyze the impact factors on response rates of surgical patients. Results The lung cancer patients' overall response rate of one thousand six hundred and eight cases was 83.1%.Reservation phone type, phone number,family history of cancer are major factors affecting lung cancer patients' telephone followed up response rate(P<0.05). Conclusions Telephone follow-up of patients with lung cancer response rates is at a low level.To improve tumor response rates of follow-up and follow-up quality is a long and arduous task,requiring medical personnel continue to learn professional knowledge,summing up a reasonable follow-up methods,but also needing patients and their families to understand and cooperate.

OBJECTIVE:To investigate the protective effect of Compound ginkgo leaf granules on liver function damage of acute pancreatitis. METHODS:Totally 82 patients with severe acute pancreatitis(SAP)complicated with liver function damage se-lected from our hospital during Sept. 2013-Sept. 2015 were randomized into control group and observation group according to ran-dom number table,with 41 cases in each group. Control group was given SAP related routine therapy. On the basis of the control group,observation group was additionally given Compound ginkgo leaf granules 5 g added into warm boiled water 80 mL via naso-gastric tube,tid,one week as a treatment course,for 2 courses. The remission time of abdominal pain,serum amylase recovery time,length of hospital stay and adverse drug reactions were observed in 2 groups. The levels of serum amylase,ALT,TBIL, AST,γ-GT,ALB,IL-6,TNF-α,creotoxin concentration,MDA,SOD,GSH-Px,total antioxidant capacity(TAC)and peripher-al blood PMN NF-κB levels were determined in 2 groups before and after treatment. RESULTS:In control group,3 patients with-drew from the study and 38 patients completed the study;in observation group,6 patients withdrew from the study and 35 patients completed the study. The remission time of abdominal pain,recovery time of serum amylase and the length of hospital stay in obser-vation group were significantly shorter than control group,with statistical significance (P<0.05). Before treatment,there was no statistical significance in the levels of serum amylase,ALT,TBIL,AST,γ-GT,ALB,IL-6,TNF-α,creotoxin concentration, MDA,SOD,GSH-Px,TAC and NF-κB between 2 groups(P>0.05). After treatment,the levels of serum amylase,ALT,TBIL, AST,γ-GT,IL-6,TNF-α,creotoxin concentration,NF-κB and MDA in 2 groups were decreased significantly,while the levels of ALB,SOD,GSH-Px and TAC were increased significantly;the observation group was significantly lower or higher than the con-trol group,with statistically significant (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Compound ginkgo leaf granules can significantly decrease serum amylase,protect liver function, with good safety. The mechanismmay be inhibiting the release of inflammatory factors,improving oxidative stress reactions,reduce the concentration of creotoxin and inhibiting the activity of NF-κB.

Objective Based on the previous research that the ethanolic extract from traditional Chinese medicine fructus forsythiae (Lianqiao) can obviously inhibit cancer cells in vitro, the article aimed to investigate the anti-proliferation effects of dammar-24-ene-3β-acetate-20S-ol (DM) extracted from fructus forsythiae on gastric cancer cells and its mechanism.Methods MTT assay was used to assess the anti-proliferation effects of DM on gastric cancer cells including SGC-7901, BGC-823, and MKN-45 in vitro.There were MKN-45 control group and its low dose and high dose groups, BGC-823 control group and its low dose and high dose groups, SGC-7901 control group and its low dose and high dose groups in the experiment.Flow cytometry was used to analyze the cell apoptosis rate.Cellquest software was used to analyze the results and record the ratio of cells at different cycles.DCFH-DA probe was applied to detect the ROS levels of blank control group, docetaxol group and DM group.The reaction system of microtubule assembly test was set with 10?mol/L docetaxol, 50 or 100 μmol/L DM final density and no medicine in blank control group.The readings of UV spectrophotometer were recorded.Microtubule assembly assay and microtubule immunofluorescence staining were applied to investigate the effects of DM on microtubule system.Results The inhibition ratio of 50 μg/L DM on the proliferation three gastric cell lines were all above 80%, with IC50s of MKN-45 11.72±1.35 μg/mL, BGC-823 17.19±0.82 μg/mL, SGC-7901 7.55±0.79 μg/mL.8 days′ low density culturing at 48 hours after 2 μg/mL DM treatment, compared with control group, the number of cell clones significantly reduced without much change in clone size, while 48 hours after 10 μg/mL DM treatment, besides a few clones of BGC-823, there were just several megascopic clones of SGC-7901 and MKN-45.In comparison with apoptotic cell ratio in MKN-45 control group[(21.1±2.5)%], its low dose group and high dose group resulted in significant rise of apoptotic cell ratio[(25.1±1.3)% and (55.2±2.3)%] (P<0.01).Compared with apoptotic cell ratio in BGC-823 control group[(13.2±2.5)%], its low dose group and high dose group resulted in significant rise of apoptotic cell ratio[(18.2±2.1)% and (41.8±2.1)%] (P<0.01).Compared with apoptotic cell ratio in SGC-7901 control group[(10.5±1.8)%], significant rise was observed in its high dose group[(41.8±2.1)%] (P<0.05) while no statistical significance was found in its low dose group[(12.3±1.6)%] (P>0.05).In comparison with MKN-45 control group, the ratio of cells at S phase decreased in its low dose group[(14.5±2.7)% vs (12.3±3.3)%,P>0.05].In comparison with BGC-823 control group, the ratio of cells at S phase increased in its low dose group[(12.2±5.4)% vs (20.2±2.1)%,P<0.05].In comparison with SGC-7901 control group, the ratio of cells at S phase increased in its low dose group[(21.5±3.8)% vs (31.3±2.6)%,P<0.05].From the detection of intracellular active oxygen after DM treatment, dose-dependent ROS level increased in all three cell lines 48 hours after 10μg/mL and 50μg/mL DM treatment.From the results of microtubule immunofluorescence staining, 48 hours after the treatment of IC50 docetaxol and 10μg/mL DM, the fluorescence signals were in local concentration and disorder.Conclusion Dammar-24-ene-3β-acetate-20S-ol demonstrated anti-proliferation effects due to the apoptosis induced by cell cycle arrest at S phase.

Objective To survey the compliance degree of patients with allergic rhinitis completing their specific desensitizing therapy and to evaluate the relating factors with this compliance degree, and to explore the nursing countermeasures. Methods A retrospective study was carried out in 186 patients with allergic rhinitis receiving specific immunotherapy from January 2007 to December 2008. Noncompliance patients were contacted with scheduled telephone interviews to investigate the causes. Results Compliance rate was 71.0%,primary reason for non-compliance is poor efficacy (16 patients, 29.6% ), followed by busy work or study (10 patients, 18.5%) and disappearance of symptoms (7 patiens, 13.0%). Conclusions The compliance degree of AR patients receiving specific desensitizing therapy is still poor, but the reasons for non-compliance can be controlled by many factors, compliance is still much room for improvement.

Objective To probe into the status and development trend of ebola,so as to provide reference for further research.Methods This paper took the ebola literature as the object of study,took GoPubMed as the retrieval and statistical tool,and conducted information analysis of the subjects,time,sources,distribution,authors and literature development trends of ebola research.Results The main subjects of ebola literature involved ebola virus and hemorrhagic fever,ebola.There has been a sharp increase on the literature number of ebola in the recent two years,particularly in 2014.The majority of literature came from the United States,and Britain ranked the second.The sources of ebola literature were mainly the SCI journals published in the United States and British.Conclusions GoPubMed can provide a vivid picture of the current situation and development trend of ebola research.Further improvements are needed both in the quality and quantity of ebola research in China.

Objective To probe into the status and development trend of avian influenza,so as to provide reference for further research.Methods The article took the avian influenza literature as the object of study,took Gopubmed as the retrieval and statistical tool,made information analysis of the subjects,time,sources,distribution,authors and literature development trends of avian influenza research.Results The main subjects of avian influenza literature involved influenza in birds and influenza,human.There has been a sharp increase on the literature number of avian influenza in the recent decade with the most in 2006.The United States accounted for the most literature and China ranked the second place.The sources of avian influenza literature were mainly the SCI journals from the United States and European countries.Conclusions Gopubmed can show a vivid picture of the current situation and development trend of avian influenza research.Further improvements are needed both in the quality and quantity of avian influenza research in China

Objective To investigate the nutrition status and quality of life(QOL)in the perioperative patients with laryngeal cancer and to analyze the effect of nutrition status on quality of life.Methods Sixty-six patients with laryngeal cancer were surveyed respectively by self-designed general situation questionnaire,nutritional risk srcreening 2002(NRS2002)and European organization for research and treatment of cancer quality of life core questionnaire(EORTC QLQ-C30).The impact of nutritional status on QOL was analyzed by the multiple linear regression analysis.Results The total score of nutritional risk of perioperative patients with laryngeal cancer was 3.53±1.51 and the incidence of nutritional risk was 28.79%.The nutrition status influenced on QOL, physiological function,cognitive function and pain area(P<0.05).Conclusion Nurses should assess the nutritional status of the patients and correct malnutrition without delay in order to improve the physical function and QOL of the patients.

Objective To explore the information distribution of PM2.5 Paper in Foreign Language.Methods This article took PM2.5 papers as research object,used GoPubMed as information search and statistical analysis tool to conduct information mining of PM2.5 literature,including subject,time,author,source,distribution and number of publications over time.Results There was steady development of PM2.5 literature published in foreign language from 2004 to 2012,dramatic increase during the year 2013 to 2015.The three top publishing years of PM2.5 literature were 2015,2014 and 2013.Main authors of such literature include Chow J,Watson J,Koutrakis P and so on.Main research countries include United States,China,Canada,etc.Big cities,like Beijing,Shanghai,Boston were main places conducted such PM2.5 research.Related articles mainly published in journals such as J Air Waste Manag Assoc,Environ Sci Technol,Sci Total Environ and so on.Six out of the top ten research journals have higher impact factors than the average level of all journals of SCI in 2014.Main research terminologies include particulate matter,air pollutants,particle size and so on.Conclusions Generally,the PM2.5 literature over the world has a high level academically and China plays a significant role in such research.