OBJECTIVE:To determine the content of epirubicin in human plasma by RP-HPLC.METHODS:The chromatographic separation was performed on YWG C18 column.The mobile phase consisted of water-acetonitrile(12∶7,with pH adjusted to 3.90?0.05 using 85% phosophoric acid)at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 254 nm.RESULTS:The linear range of epirubicin was 0.12～4.80 mg?L-1(r=0.998 4)with the lowest detective range of 0.01 mg?L-1.The intraday RSD was less than 4.40% and the inter-day RSD was less than 5.60%,and the average recovery rate was 99.40%(RSD=4.02%).The sample solution was stable within 12 h under room temperature.CONCLUSION:The method is easy,precise and reproducible to operate and the result of the determination is accurate.

Objective To investigate the effects of vascular endothelial growth factor-C(VEGF-C)and VEGF-R3(fms-like tyrosine kinase-4,Flt-4)on lymphangiogenesis in adenocarcinoma of rectum.Methods A series of 32 adenocarcinoma of rectum were detected for the expression of VEGF-C and VEGF-R3(fms-like tyrosine kinase-4,Flt-4)and Podoplanin by immunohistochemical SABC methods.Results The density of lymphatic vessel in the perimeter of the adenocarcinoma tissue of rectum was much higher than in the interior(P

Objective: To observe influence of sensation of uncertainty in illness on curative effect of two-week rehabilitation program in patients with acute myocardial infarction (AMI). Methods: A total of 85 AMI patients recently treated in our hospital were selected continuously. They received two-week rehabilitation program treatment and were assessed by Missals uncertainty in illness scale (MUIS). According to MUIS scores, AMI patients were divided into middle-high score group (74.8~117.4 scores, n=51) and low score group (32~74.7 scores, n=34), and they were compared with 43 patients with coronary heart disease (CHD) angina pectoris (CHD control group) received treatment in the same period. Results: Compared with CHD control group, there were significant increase in each dimension score and total score of MUIS [total score (60.61±12.42) scores vs. (78.34±15.20) scores]in AMI patients (P<0.05~0.01). The exercise peak heart rate of MUIS middle-high score group was significantly higher than that of low score group [(137.80±26.49) times/min vs. (126.12±20.51) times/min, P<0.01]. Compared with low score group, there were significant decrease in total scores of activity of daily living (ADL) scale [(84.15±16.38) scores vs. (73.92±14.21) scores] and the 36-item short-form general heath survey [SF-36, (45.22±6.86) scores vs. (37.95±6.43) scores], and significant increase in total score of symptom checklist (SCL)-90 [(138.35±36.47) scores vs. (151.87±42.61) scores], mean days in CCU [(2.53±0.26)d vs. (2.77±0.29)d], mean days on bed [(4.46±0.25)d vs. (5.38±1.22)d], mean hospital day [(20.48±3.16)d vs. (25.37±3.82)d] and mean inpatient fee [(39.1±8.2) thousand RMB vs. (45.7±9.3) thousand RMB] in middle-high score group, P<0.05~0.01. Conclusion: There is significant sensation of uncertainty in illness in patients with acute myocardial infarction, and it makes curative effect of two-week rehabilitation program significantly decrease.

At present the study of Marine antineoplastic drugs has already become the focus of concern around the world, Marine natural products has become an important source of clinical anti-cancer drugs. Ecteinascidin-743 from the Caribbean sea squirts Ecteinascidia turbinata extraction have been the ocean alkaloid, antitumor activity is significantly higher than the current widely used clinical antitumor drugs, inhibit a wide variety of tumor cell activity such as leukemia, ovarian cancer, soft tissue sarcoma, melanoma, cervical cancer, breast cancer, colorectal cancer, kidney cancer, prostate cancer, lung cancer and non-small cell lung cancer and other tumor cells, is the first successful application to the oceans, clinical antitumor drugs. In this paper, the drug synthesis, pharmacological and clinical research progress were reviewed.

OBJECTIVE: To provide meaningful reference for the improvement of full-time clinical pharmacists' ability.METHODS: According to clinical practices,the experience of author engaging in clinical pharmacy was introduced.RESULTS & CONCLUSION: Clinical pharmacists should take a correct attitude and practice their profession as best as they could,based on the professional orientation.Scientific research should be linked with problem settlement to promote the growth of clinical pharmacists.

Objective To observe the alteration of skin complexion after UVA and UVB exposure.Methods The back skin of ten females with skin type Ⅲ was subjected to single exposure to solar-simulated UVA of double minimal persistent pigment darkening (MPPD) or UVB of double minimal erythema dose (MED). Skin reflectance was assessed with clinical grading, spectcolometer and Mexameter MX 18 before irra-diation, 6 hours, 1, 7 and 14 days after the irradiation. Results After UVB irradiation, a~* value and erythema index (EI) abruptly increased at 6 hours and peaked on day 2; L~* value sharply declined on day 1; ITA° markedly decreased on day 7; melanin index (MI) declined within the first 2 days, but notably increased on day 7. After UVA irradiation, a~* and El value experienced no apparent changes; L~* value obviously declined at 6 hours; ITA° reached its lowest value on day 14; MI increased only on day 1. Conclusions There is a significant difference in the kinetics and extent of skin complexion changes after UVA and UVB irradiation. EI and a~* value are sensitive and accurate indices for evaluating sunburn, and MI and ITA ° for analyzing tanning.

Objective To discover the reciprocal regulation and its molecular mechanism of estro-gen, IL-6 and IL-8 in ovarian cancer cells. Methods Based on our previous studies, the effect of 17β-estradiol (E2) on the expression levels of IL-6, IL-8 and their respective receptors was investigated. Mean-while, the effect of IL-6/IL-8 on estrogen receptor (ER) expression and estrogen-dependent transcriptional activation was analyzed. Gene expression profile analysis revealed that CAOV-3 and OVCAR-3 cells, which express ER, IL-6 and IL-8 receptors, were suitable models for this study. Results We found that E2 not only enhanced IL-6/IL-8 secretion via NF-κB signaling pathway, but also modulated IL-6 and IL-8 receptors expression. Tamoxifen (Txf), an ER antagonist, completely abolished E2-stimulated IL-6/IL-8 expression. On the other hand, in the absence of estrogen, both cytokines increased ERα expression, decreased ERβ ex-pression, and activated estrogen-dependent transcriptional activation, which was completely blocked by Txf. Pretreatment of OVCAR-3 with p38 MAPK, MEK1/2 or ErbB2 MAPK inhihitors, respectively, IL-6-media-ted ER activation was blocked, while IL-8-indueed ER activation was blocked by Src inhibitor. Conclusion These data suggest that estrogen, IL-6 and IL-8 may form a mutual amplifying signaling which contributes to the growth and development of ovarian carcinoma.

Objective To explore the relationship between skin photo reactions and structural pa-rameters. Methods Benzophenone-1, Benzophenone-2 and Benzophenone-3 were assessed by phototoxici-ty test and photoallergic test on guinea pig. And the phototoxicity index and photoallergic index were cal-culated after clinical assessment. Structural parameters of the three sunscreens like total energy, binding energy, electronic energy, heat of formation, dipole and sum of the net charges were also calculated by the soft of HyperChem7.0, respectively. Results The phototoxicity index rised with total energy, elec-tronic energy and sum of net charges increasing. And the photoallergy index rised only with binding ener-gy increasing. So there was some correlation between phototoxicity index and total energy, electronic en-ergy and sum of net charges (P<0.01). Significant relationship was only between the photoallergy index and binding energy (P<0.01). Conclusions The phototoxicity is correlated with total energy and e-lectronic energy, while photoallergy is related to binding energy. It is possible to establish a model evalua-ting the safety and structural parameters of benzophenone ramifications.

OBJECTIVE:To provide reference for the exploration of training model of clinical pharmacist in according with special condition of our country.METHODS:The manner of clinical pharmacist training and pharmaceutical care in the training base of our hospital were analyzed,and training contents and pattern were summarized.RESULTS&CONCLUSIONS:It needs to improve the capacity of students during clinical pharmacist training in respect of theory and practice.Moreover,strict access system and examination should be strengthened,teaching and studying should complement each other.

OBJECTIVE: To probe into the status quo of hospital pharmacy practice in Japan and to provide reference for the further improvement of hospital pharmacy model in China.METHODS: Hospital pharmacy practice in affiliated hospital of Nippon Medical School was introduced,and the development of hospital pharmacy practice in Japan in the past few decades was also investigated.RESULTS & CONCLUSIONS: There is still a certain gap in the level of hospital pharmacy between China and Japan,so it is particularly important to improve hospital pharmacy system in China by the means of defining the pharmacist's responsibility and obligation,etc.