1.Effects of different resin core materials on microleakage for post-core restorations
Lijie TANG ; Guilan ZHANG ; Zhimin ZHU
Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(4):584-586,605
Objective To compare the effects of different composite resins as core material on the degree of microleakage in post-core repairation.Methods A total of 46 recently extracted single-rooted mandibular premolars were distributed into different groups according to different core material including ParaCore,AP-X and Ceramage.Then we used direct or indirect forming method to make post-core restorations.All of the specimens were submerged in dyes.Then,they were demineralized,dehydrated and processed to be transparent.The extent of the dye leakage was examined under a stereomicroscope.Results The microleakage value was significantly higher in Group of direct-mold-cement with ParaCore (4.94± 1.71)mm than in Group of indirect-mold-secondary-cement with ParaCore (0.91 ± 0.33) mm,Group of indirect-mold-secondary-cement with AP-X (0.87 ± 0.27) mm,and Group of indirect-mold-secondary-cement with Ceramage (1.02 ± 0.34)mm.Conclusion Different methods of building and cementing FRC post-core restorations,but not different composite resins as core material,have significant effects on the extent of microleakage in post-core repair.
2.Present situation and prospect of neoadjuvant therapy for locally advanced esophageal carcinoma
Lijie TAN ; Hao WANG ; Han TANG
Chinese Journal of Digestive Surgery 2017;16(5):450-453
The morbidity and nortality of esophageal carcinoma showed an upward trend in recent years.Neoadjuvant radiochemotherapy is the nain mode of treatment for advanced esophageal carcinoma,including neoadjuvant chemotherapy,neoadjuvant radiotherapy and neoadjuvant radiochemotherapy.However,there are still many controversies on efficacies and advantages or disadvantages of treatment mode.In this article,authors will investigate the present situations and future direction of neoadjuvant therapy for locally advanced esophageal carcinoma.
3.High-content screening in studies of toxicology and its application in drug-induced hepatotoxicity
Shuai GE ; Naping TANG ; Lijie FU ; Jing MA
Chinese Journal of Pharmacology and Toxicology 2017;31(6):689-695
Drug-induced liver injury(DILI) is one of the major causes of termination of drug development.The establishment of a high-throughput test system to predict potential clinical hepatotoxicity is a valuable approach in the pharmaceutical industry.The high-content imaging-based in vitro assays allow simultaneous detection of cellular multiple parameters in the system.The real-time monitoring of multiple signaling pathways can shed light on many mechanisms of cell injury,with high sensitivity and specificity.Many types of liver cell models have been applied to high-content screening(HCS) so far.This paper introduceds the HCS technology and reviews the data of hepatotoxicity obtained from HCS technology in recent years.At the same time,we discuss the application of this technology in exploring the mechanism of hepatotoxicity and the potential of HCS technology in studying DILI and mechanisms.
4.Effects of different fabricating methods on the microleakage for preformed fiber posts
Lijie TANG ; Chen CHEN ; Zhimin ZHU ; Hongchen LIU
Journal of Practical Stomatology 2017;33(1):117-119
To compare the microleakage of fiber post and resin-core system fabricated by different methods.The roots of 36 recently extracted single-rooted mandibular premolars were undergone endodontic treatment.Fiber posts and Paracore flowable resin composite were used for fabricating post-core restorations.Microleakage was examined by dye penetration method.The microleakage value was significantly higher in Direct-Mold-Cement-Method group(4.94 ± 1.71) mm compared to Direct-Mold-Secondary-Cement-Method group(0.91 ±0.33) mm and Indirect-Mold-Secondary-Cement-Method group (0.87 ± 0.27) mm (P < 0.05).
5.Effect of Interventional Therapy of Urokinase on Sudden Hearing Loss
Libin TANG ; Tangying LI ; Liangcai GAO ; Jicai SUN ; Lijie ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2007;13(4):395-396
Objective To observe the effect of interventional therapy of urokinase on sudden hearing loss.Methods65 patients with sudden hearing loss were divided into the treatment group (n=35) and control group (n=30). The patients of the treatment group were treated with urokinase pouring into vertebral artery accordingly into labyrinthine artery in order to improve internal ear microcirculation. Those of the control group were treated with routine drug dropping in vein. All patients of two groups were examined by pure tone test before and after treatment and the therapeutic effect of two groups was compared.ResultsIn the treatment group, the audition of 21 cases recovered, 12 cases got 15~30 dB increasing, 2 cases increased <15 dB. In the control group, the audition of 10 cases recovered, 4 cases got 15~30 dB increasing, 9 cases increased <15 dB and 7 cases had no obvious improvement. There was a significant difference between effects of two groups ( P<0.05).ConclusionThe effect of urokinase on sudden hearing loss is superior to routine drug dropping in vein.
6.Experimental Study of Antitoxic Effect of Sodium Selenite on Guinea Pig Cochlea Injury Induced by Gentamicin in Vivo
Liang-cai GAO ; Tang-ying LI ; Hong LU ; Libin TANG ; Lijie ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(2):131-132
ObjectiveTo explore the effect of sodium selenite on gentamicin-induced ototoxicity.MethodsTwenty guinea pigs were randomly divided into the experimental group and control group with 10 animals in each group. The animals were treated with i.m injection of gentamicin (200 mg/kg/d for 8 consecutive days, and at same time, the experimental group was added with p.o tablet of sodium selenite (1 mg/kg/d). Before and after the administration, hearing function was evaluated by examination for auditory brain stem responses (ABR). The cochlear outer haircells were observed by scanning electronic microscope (SEM) and transmission electronic microscope (TEM).ResultsABR threshold of the control group was about 30 dB above that of the experimental group (P<0.01). The latency of waveⅠof the control group was about 0.23 ms above that of the experimental group (P<0.01). Under SEM, the cilia of the majority outer hair cells of the control group lodged even disappeared, while that of the experimental remained regular. Under TEM, in outer hair cells of the control group, mitochondrial crests were obscure, out-membrane was damaged and local protruding, the number of secondary lysosomes was increased, myeloid bodies appeared, but in the experimental group, outer hair cells basically remained normal.ConclusionSodium selenite has antitoxic effect on guinea pig cochlea injury induced by gentamicin in vivo.
7.Expression and biological activity of porcine interleukin-18 in recombinant Lactococcus lactis.
Lu MA ; Xinyuan QIAO ; Lijie TANG ; Yanping JIANG ; Wen CUI ; Yijing LI
Chinese Journal of Biotechnology 2014;30(10):1541-1548
To obtain active protein of pIL-18 expression in Lactococcus lactis, and to observe its biological activity, the total RNA was extracted as template from peripheral blood mononuclear cells. Porcine interleukin 18 (pIL-18) was amplified by RT-PCR. The resulting fragment was cloned into pAMJ399 L. lactis vector, and then transformed to L. lactis MG1363 cells by electroporation. Expression of pIL-18 protein was detected by SDS-PAGE and Western-blotting. Bioactivity of the product was tested by pig spleen lymphocyte proliferation test and cytopathogenic effect inhibition assay. The result of Western blotting and bioactivity test shows that the molecular weight of pIL-18 protein was 19 kDa. The react line was observed in both supernatant and precipitated of the recombinant bacteria pAMJ399-pIL18/MG1363. The expressed pIL-18 can promote the proliferation of pig spleen lymphocyte, and significantly inhibit virus multiplication. As conclusion, porcine interleukin-18 was successfully expressed in L. lactis, and the product was biologically active.
Animals
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Blotting, Western
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Electrophoresis, Polyacrylamide Gel
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Electroporation
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Genetic Vectors
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Interleukin-18
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biosynthesis
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Lactococcus lactis
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metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Swine
8.Comparison of expression and antibacterial activities of recombinant porcine lactoferrin expressed in four Lactobacillus species.
Hui YU ; Yanping JIANG ; Wen CUI ; Xiao WU ; Jia HE ; Xinyuan QIAO ; Yijing LI ; Lijie TANG
Chinese Journal of Biotechnology 2014;30(9):1372-1380
The coding sequence for the mature peptide of porcine lactoferrin (Plf) was synthesized according to the codon usage of lactobacillus, to establish optimized porcine lactoferrin Lactobacillus expression system. The gene was ligated into the Xho I/BamH I site of Lactobacillus expression vector pPG612.1 and the recombinant plasmid pPG612.1-plf was transformed individually into Lactobacillus casei ATCC393, Lactobacillus pentosus KLDS1.0413, Lactobacillus plantarum KLDS1.0344 or Lactobacillus paracasei KLDS1.0652 by electroporation. After induction with xylose, expression of the recombinant proteins was detected by Western blotting and confocal laser scanning microscopy. Secretion of recombinant Plf proteins from four recombinant species was determined quantitatively by ELISA. The antibacterial activities of recombinant proteins were measured by agar diffusion method. The result shows that Plf was correctly expressed in four species of recombinant lactobacillus, with molecular weight of about 73 kDa. The expression levels in recombinant Lactobacillus casei, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paracasei were 9.6 μg/mL, 10.8 μg/mL, 12.5 μg/mL and 9.9 μg/mL, respectively. Antimicrobial activity experiment shows that the recombinant proteins were active against E. coli, Staphylococcus aureus, Salmonella typhimurium, Listeria, Pasteurella. The recombinant Plf expressed by recombinant Lactobacillus plantarum showed the best antibacterial activity among all recombinant lactobacillus species. These data represent a basis for the development and application of porcine lactoferrin from recombinant lactobacillus.
Animals
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Anti-Bacterial Agents
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biosynthesis
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Lactobacillus
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metabolism
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Lactoferrin
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biosynthesis
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Recombinant Proteins
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biosynthesis
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Swine
9.Impact of heme oxygenase 1 on monocyte chemoattractant protein 1 expression of human umbilical vascular endothelial cell in uremic milieu
Lijie GU ; Yixin HUANG ; Weijie YUAN ; Jun LIU ; Zhihuan TANG ; Qing YU ; Jian YAO
Chinese Journal of Nephrology 2008;24(8):566-570
Objective To investigate the influence of uremic serum on the monocyte chemoattractant protein 1 (MCP-1) expression of human umbilical vascular endothelial cells (HUVECs) in vitro and the effect of up-regulation of berne oxygenase 1 (HO-1) on the synthesis of MCP-1 of HUVECs in uremic milieu. Methods HUVECs were incubated to confluence and then preineubated with heroin and/or protoporphyrin zinc IX (ZnPP)for 6 hours.The cultures were subsequently incubated with M199 cell medium containing 10% serum of healthy people or with medium containing 10% serum of maintenance hemodialysis (MHD) patients.HO-1 protein and mRNA expression was detected by immunohistochemistry and semi-quantitative RT-PCR.MCP-1 mRNA expression was measured by semi-quantitative RT-PCR,and MCP-1 protein was quantified by ELISA. Results Up-regulated expression of MCP-1 mRNA and protein was detected in HUVECs incubated with medium containing 10% serum of MHD patients.The protein synthesis was 2.95 folds of the control.Heroin induced expression of HO-1 mRNA and protein,and concurrently inhibited the up-regulated MCP-1 expression induced by uremic serum.Such effects of heroin could be blocked by ZnPP. Conclusions Uremic serum induces the expression of MCP-1 in HUVECs.Up-regulated expresson of endothelial HO-1 induced by heroin inhibits the enhancement of MCP-1 synthesis.HO-1 may be beneficial to the alleviation of endothelial cell injury in uremic milieu.
10.Survey of mental health and social support of medical staff in general hospitals
Lei HUA ; Xiangping WANG ; Hongdong BAI ; Lijie ZHAO ; Zhe TANG ; Youqin LIU ; Hua XING ; Zhuo WANG
Chinese Journal of Hospital Administration 2010;26(6):449-453
Objective To probe into the social support, coping styles, and mental health status of medical staff in a tertiary hospital in Beijing, for identifying potential mental problems. Methods Such means as the Social Support Rating Sheet (SSRS), Simple Coping Style Questionnaire (SCSQ), Symptom Checklist (SCL-90), and demographic characteristics questionnaire were called into play, to study 2460 medical staff in five tertiary hospitals in Beijing. Results Differences were identified among medical staff of different demographic characteristics, in terms of positive coping style and support dimensions. Analysis of the survey data indicates that the total SCL scores, total symptom problem index, positive symptom pain level, total average score of the factors, are positively related to passive coping style, as P<0. 01; and are negatively related to subjective/objective support, support utilization, and total support scores, as P<0. 01. Conclusions Hospital administration is expected to implement focused intervention to such medical staff as those under 40 years old, women, widows or divorcees, those with education lower than university degree, those with junior academic title or lower, those without titles, those in emergency departments and emergency intensive care units, doctors and nurses, frequent night-shift workers, those of excessively low self evaluation, and medical staff with chronic diseases. The purpose of this practice is to alleviate their mental stress.