1.Immune Responses to Challenge Infection in Mice Immunized with Trichinella spiralis Adult Worm Soluble Antigen
Lijie SHEN ; Zhiyong LUO ; Shenghua ZHU ;
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(05):-
Objective To study the immune response induced by the mice immunized with Trichinella spiralis(T.spiralis) adult worm soluble antigen(AWSAg). Methods T.spiralis AWSAg was prepared to immunize Kunming strain mice. Dynamic changes of IgG, IL 2 and T lymphocyte subsets from immunized mice were determined after challenge infection on d 7, d 14 , d 21 , d 28 and d 35 . Results On d 7 after challenge infection, IgG and CD4 + T cells of immunized group were markedly elevated and persisted higher over the observation period. In contrast, CD4 + T cells and CD4/CD8 ratio were significantly decreased in unimmunized group resulted from immune suppression after infection. IL 2 levels reached the peak on d 7 and persisted in high level from d 7-d 21 in both immunized and unimmunized group after infection, then decreased gradually. Till 35 days after infection, IL 2 level was still higher than the normal mice. Conclusion Mice immunized with AWSAg of T. spiralis produced a potential cellular and humoral immune response.
2.OBSERVATION OF THE KINETIC CHANGE ON IgG,T-CELL SUBSETS,IL-2 LEVEL OF MICE INFECTED TRICHINELLA SPIRALIS
Lijie SHEN ; Shenghua ZHU ; Zhiyong LUO
Chinese Journal of Zoonoses 2000;(6):70-71,66
AimTo explore the kinetic change of the IgG,T-cell subsets and IL-2 level from the mice infected with T. Spiralis. Methods The level of specific IgG A band IL-2 was determined by ELISA,the percentage of CD4+and CD8+ T-cells were examined by flow cytometry on 7, 14,21,28,35 days after mice infected with T. spiralis respectively. ResultAfter infected with T. spiralis,the level of IgG in mice rised gradually,and reached its peak on the 35th day. The change of T-cell subsets showed that CD4+T cells decreased,which CD8+Tcells increased. The ration of CD4+/CD8+cells decreased,and which was the obvious on the 14thday. It did not recover to normal level even on the 35th day. The IL-2 of level reached the peak on the 7th day after infected,then IL-2 level decreased quickly and lower than that of normal mice on 35th day after infected. Conclusion When the acute phase of T. spiralis infection,the immune function of host was inhibited. The protective immunity of against T. spiralis infection was cellular immunity mainly ,in cooperation with humoral immunity.
3.Somatostatin receptor scintigraphy of orbital inflammatory pseudotumor associated with systemic vasculitis
Sha LUO ; Li CUI ; Lijie LIU ; Jinfeng LI ; Mei LI
Chinese Journal of Nuclear Medicine and Molecular Imaging 2015;35(3):190-194
Objective To explore the value of somatostatin receptor scintigraphy(SRS) in evaluating the immune activity of orbital inflammatory pseudotumor associated with systemic vasculitis.Methods Twenty-five patients with orbital inflammatory pseudotumor associated with systemic vasculitis (10 males,15 females,average age:(51.2± 14.2) years) underwent SRS.The uptake ratio (UR) of orbital inflammatory pseudotumor was obtained.(1) Patients were divided into group A (with immune activity) and group B (without immune activity) according to Birmingham vasculitis activity score (BVAS).The difference of UR between the 2 groups was compared by two-sample t test.The difference of UR before and after treatment in 12 patients was also compared.(2) Based on the results by BVAS,ROC curve was used to obtain the cut-off value of UR,as well as the diagnostic efficiency and Youden index.The consistency between SRS and BVAS was calculated.(3)Patients were divided into two groups according to the cut-off value of UR and the prognosis difference between them was compared by Fisher exact test.(4)The expression of SSTR2 and SSTR5 was observed by immunohistochemistry.Results (1) UR in group A was significantly higher than that in group B (2.09±0.44 vs 1.32±0.46,t =5.94,P<0.01).After glucocorticoids treatment,the UR in group A reduced significantly (t=4.07,P<0.01),but not in group B (t=1.76,P>0.05).(2)ROC curve analysis identified UR cut-off value as 1.66,with the sensitivity of 87.5%,specificity of 95.7%,positive predictive value of 95.2%,negative predictive value of 88.0%,accuracy of 91.3% and Youden index of 83.2%.The consistency between SRS and BVAS was strong (Kappa =0.840).(3) The prognosis was significantly different between patients with UR≥ 1.66 and UR<1.66 (P<0.05).(4) The immunohistochemical results revealed high expression of SSTR2 and SSTR5 in inflammatory cells in patients with immune activity.Conclusion SRS has potential value in evaluating the immune activity of orbital inflammatory pseudotumor associated with systemic vasculitis.
4.Study and application of surface antigen in tachyzoites of Toxoplasma gondii
Li LENG ; Mi LUO ; Ju GAO ; Lijie SHEN
Chinese Journal of Schistosomiasis Control 2014;(6):687-689
Toxoplasma gondii is an intracellular protozoan parasite that infects all warm?blooded animals. The surface anti?gens of T. gondii with the potential for application as antigens of diagnosis and vaccines have been studied extensively in recent years especially for P43 P35 P30 P23 and P22. The studies on the surface antigen in tachyzoites of T. gondii are reviewed in this paper.
5.Optimization of Extraction Process of Resveratrol from Polygonum Cuspidatum Based on Central Composite Design and Response Surface Method
Lijie WANG ; Jianli LI ; Mingyi SHI ; Yue LUO ; Zixian CHEN
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(5):1193-1200
This study was aimed to optimize the extraction process of resveratrol by central composite design (CCD) and response surface method (RSM), based on the result of single factor experiment. Resveratrol was extracted from Polygonum cuspidatum by water bath extraction of organic solvent. And content of resveratrol was taken as index in the investigation of solvent concentration effect, extraction time and solvent content on extraction process. The results showed that the optimum condition was 12.7 times amount of 72.0% ethanol and extract for 2 times under the temperature of 50℃, 1.1 h for each time; the average deviation between the maximum theoretical value and measured value was 0.83%. It was concluded that this extraction process was highly predictive, which provided experimental evidence for the industrial production of P. cuspidatum extraction.
6.Advanc in research of the effects of Drebrin on synaptic plasticity and related cognitive dysfunction
Lijie JIA ; Yan LUO ; Fujun ZHANG ; Buwei YU
Chinese Pharmacological Bulletin 2003;0(08):-
As the neuron-specific actin binding protein,Drebrin ( developmentally regulated brain protein) can affect spiny and synaptic morphology and function by changing the property of cytoskeleton,and modulate synaptic plasticity. Neural excitability regulates expression and activitiy of Drebrin through a variety of signaling molecules,making Drebrin' s function correlated with that of neurons. Under pathological conditions,the abnormal Drebrin affects synaptic plasticity,which leads to different degrees of cognitive dysfunction,and it is closely relates to the progress of cognitive dysfunction. Extensive studies of physiological and pathological functions of Drebrin in overall and molecular ways will not only contribute to a thorough understanding of cognitive dysfunction,but also develop the new target of therapeutics for cognitive dysfunction.
7.Quality standard for Xintongning Tablets
Yanping LUO ; Jian CHEN ; Lijie HUANG ; Hanshen ZHEN
Chinese Traditional Patent Medicine 1992;0(02):-
AIM: To establish the quality sdandard for Xintongning Tablet(Radix et Rhizoma Ginseng,Radix et Rhizoma Salviae Miltiorrhizae,etc.) METHODS: TLC was used to indentify Radix et Rhizoma Salviae miltiorrhizae,Rhizoma Curcumae Longae and Fel Ursi Powder.The contents of ginsenoside Rg_1,Re were determined by HPLC. RESULTS: Radix et Rhizema Salviae miltiorrhizae,Rhizoma Curcumae Longa and Fel Ursi Powder could be indentified by TLC.The average recoveries of ginsenside Rg_1,Re were 97.18% and 96.74% respectively(n=5). CONCLUSION: The methods are reliable and reproducible.It can be used for quality control of Xintongning Tablets.
8.Comparation of Toxoplasma gondii separated from HIV-positive people and RH strain GRA6 gene
Lingjuan CHEN ; Yuxi JIA ; Li LENG ; Mi LUO ; Ju GAO ; Wei LI ; Lijie SHEN
Chinese Journal of Schistosomiasis Control 2014;(4):434-436
Objective To comparatively analyze Toxoplasma gondii separated from HIV-positive people and RH strain GRA6 gene. Methods By using the nested PCR the amplification of Dali HIV-positive blood samples and RH strains of Toxo-plasma GRA6 genome was performed. The GRA6 gene amplification positive product was selected and the electrophoresis imag-ing was performed by being digested with the Mse I endonuclease and the gene sequences were measured and analyzed. Re-sults The GRA6 gene fragment 800 bp was successfully amplified and about 600 bp and 200 bp bands were got by Mse I. The sequencing results showed that T. gondii GRA6 gene positive samples had 2 nucleotide variation compared with T. gondii strain RH namely 447 base pair at C becoming G and 623 base pair at G becoming T. At 146 bp and 690 bp the Mse I restric-tion sites TTAA were found. Conclusion The preliminary judgment shows that the Dali HIV-positive T. gondii genotype is consistent with RH strain belonging to genotype I.
9.Analysis of sites of Toxoplasma gondii SAG2 gene in blood of HIV-positive people in Lincang City,Yunnan Province
Yuxi JIA ; Lingjuan CHEN ; Wei LI ; Daping NIE ; Mi LUO ; Jianfang HE ; Lijie SHEN
Chinese Journal of Schistosomiasis Control 2015;(1):32-35
Objective To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV?posi?tive persons in Lincang City,Yunnan Province. Method Two segments of SAG2 gene of T. gondii from blood samples of HIV?positive persons in Lincang City were extracted and amplified by using the nested PCR method and the genotype was identified and compared with the standard strain(Type I)of Toxoplasma gondii. Results Thirty?five SAG2 genes(241 bp)and 35 SAG2 genes(221 bp)of T. gondii were amplified from 170 blood samples of the HIV?positive people,and 4 of each case were selected and digested with enzyme,then 2 aim gene fragments of each case were chosen and compared with the standard strain (Type I)of T. gondii. The digestion of SAG2 gene(241 bp)showed the genotype of the blood samples was Type I or Type II, and the digestion of SAG2 gene(221 bp)confirmed that the genotype was Type I. Conclusion It is preliminarily confirmed that the genotype of T. gondii in blood of HIV?positive persons in Lincang City,Yunnan Province is Type I.
10.Inhibitory Effect of Serum Containing Modified Fuzheng Yiliu Decoction on Human Colon Cancer Cell Line HT-29 and Its Mechanism
Wei WANG ; Haigan YANG ; Lijie LUO ; Yupeng LIANG ; Yansheng ZHENG ; Dechang DIAO
Journal of Guangzhou University of Traditional Chinese Medicine 2014;(6):979-982,1001
Objective To study the effect of Chinese medicinal compound recipe ( modified Fuzheng Yiliu Decoction, MFYD) on the proliferation of colon cancer HT-29 cells and on the expression of cellular apoptosis gene, Bcl-2, and tumor-inhibiting gene, P53. Methods Colon cancer HT-29 cells were divided into serum containing MFYD groups ( treated with different concentrations of serum containing MFYD) and blank serum group. Methyl thiazolyl tetrazolium ( MTT) , real time cellular analysis ( RTCA) and reverse transcription poly merase chain reaction ( RT-PCR) were used to detect the cell proliferation and mRNA expression of Bcl-2 and P53 separately. Results The proliferation of colon cancer HT-29 cell line was inhibited by MFYD ( P<0.05) in concentration-and time-dependent manner. The Bcl-2 mRNA expression was decreased and p53 mRNA was increased markedly in HT-29 cells after co-culturing with 12% or 15% volume fraction of serum containing MFYD for 48 hours, the differences being significant compared with the blank serum group ( P<0.05). Conclusion MFYD has obvious inhibitory effect on the proliferation of colon cancer HT-29 cells, and its mechanism may be related to down-regulating the expression of Bcl-2 gene and up-regulating the expression of P53 gene.