[Objective] To investigate the relevant factors of neonatal hemolysis, especial y the association between maternal health and neonatal hemolysis. [Methods]O and Rh positive pregnant women in late pregnancy in 497 cases, make physical identification, and track whether the newborn hemolysis. [Results] There were significant differences in different physical pregnant women in the postpartum hemolysis newborn during pregnancy, the partial repletion heat, phlegm dampness postpartum hemolytic disease of the newborn was a high incidence of pregnancy, the lowest incidence rate and quality of postpartum hemolytic disease of the newborn. [Conclusion]The constitution(partial phlegm, partial heat) pregnant women for health care, to achieve peace and reduce neonatal ABO hemolytic was significant.

The performance and clinical application of the skull repair materials differ depending on their variety. Organic glass has ever been widely applied, but its biocompatibility is poor with the high infection rate of subcutaneous effusion; Bone cement is good at histocompatibility but bad at absorption, thus can be only used for parts of skull repair; Medical silica gel polyester grid is cheap and effective, however, local infection and material's expose occur, leading to unsatisfactory appearance; Titanium and Titanium-alloy, as one kind of high polymer materials, have achieved widely application on the clinical practice for repairing skull defects due to their good biocompatibility and physicochemical property, but many shortages are also observed. With the development of the society, patients require more and more, some insufficient materials for the repair have been abandoned, and the materials for repairing skull defects should be selected according to the individual condition. In this paper, various materials of the skull repair were compared concerning the property and clinical application, to search an optimal material for the skull repair, with the good biocompatibility and good clinical efficiency.

Virus hepatit is a severe infectious disease which threaten people's health.There is no specific and effective methods to treat it untill now.In recent years,gene therapy,anti-hepatitis virus,has made great progress in vitro and in animal.Gene therapy is going to be a important measure for treating virus hepatitis.However,ethical problems should be solved before appling it in clinic.

Lung cancer is currently one of the most common malignant tumors in the world, with an increasing trend of morbidity and mortality.The major causes of lung cancer are inhaled carcinogens, such as tobacco and environmental pollution, but a growing number of researches suggest that the incidence of lung cancer is closely related to genetic factors.In the past few years, great progress has been made in research on single nucleotide polymorphism of susceptibility gene in lung cancer, and the most studies that have aimed to ultimately provide scientific basis for preventing and controlling the lung cancer have focused on screening of lung cancer etiology and developing gene therapy.In this review, the new findings of genetic susceptibility in lung cancer have been summarized.

Pediatric acute liver failure is a severe and sudden onset of hepatocyte dysfunction,leading to synthetic and detoxification failure,which could progress to multi-organ failure and death.Mortality is predominantly high due to the raised intracranial pressure,infections and multi-organ failure.The mainstay of management is supportive care in an intensive care unit.The intensive care managements include immediate measures,etiology,management of complications and liver assist device,liver transplantation.Liver transplantation is an important life saving procedure for children with acute liver failure.

Objective To evaluate the effect of implantation of chitosan membrane with triamcinolone(TA) into perichoroidal space combined with intraocular vancomycin injection on the protection of retina in rabbit endophthalmitis.Methods The drug membrane was made of gelatin-chitosan.The right eyes of 30 normal pigment rabbits were inoculated intravitreally with 0.1 ml mixture of 105 CFU standard strain Staphylococcus aureus(ATCC 25923).Twenty-four hours later,rabbits were equally randomized to intravitreal vancomycin group(Group A),intravitreal vancomycin and TA eye dropping(Group B),and intravitreal vancomycin combined with TA membrane implantation(Group C).Clinical observation of ocular fundus was done every day with indirect ophthalmofundoscope.B ultrasound examination was carried out in 24 h after inoculation and on the 14th day after therapy.Electroretinogram(ERG) was done before therapy and the 14th day after therapy.Histopathologic grading and electron microscopy were carried out at the 14th day.Results After implanting the drug membrane into perichoroidal space,the inflammation in group C was markedly less than the other 2 groups,and its inflammation score was significantly lowest(P

AIM: To investigate the effect of urokinase plasminogen activator (uPA) on metastasis inhibition of gastric cancer SGC7901cells induced by parthenolide. METHODS: The changes of SGC7901 cells reproductive activity were analyzed by MTT. Light microscope was used to observe the cell morphological changes. The effects of parthenolide on migration and invasion capacity of SGC7901 cells were determined by using matrigel and transwell system. The expression of uPA was detected by Western blot and RT-PCR assays. RESULTS: Parthenolide inhibited the proliferation of SGC7901 cells in a time-and concentration -dependent manner ranging from 100 to 200 ?mol/L. Light microscopy showed the suppressing effect on growth of SGC7901 cells. After exposed to parthenolide, the migration and invasion capacity of SGC7901 cells were significantly decreased, the number of cells gradually decreased through the basement membrance. The Western blot assay showed that the expression of uPA protein declined gradually after exposed to parthenolide for various period of time. CONCLUSION: Parthenolide can inhibit the growth and metastasis activity of SGC7901 cells, and uPA played an important role in the latter process.

Nuclear factor-?B (NF-?B) is a nuclear transcription factor that regulates the expressions of multiple genes. It can be activated during cerebral ischemia; it is closely correlated with the inflammatory reaction and apoptosis during cerebral ischemia. NF-?B inhibitor ? (I?B?), an important inhibitor factor of NF-?B, can regulate the survival and death of neuron in the ischemia penumbra. Therefore, the inhibition of the degradation of I?B? and the prevention of the activation of NF-?B with drugs or molecular biological methods has become an important approach in study of the effect of NF-?B at present. Recent studies have indicated that I?B? itself may also participate in the regulation of apoptosis.

Epidemiological studies have suggested that the increase of uric acid level has a direct correlation with the risk of ischemic stroke. However,the significance of this correlation remains controversy. Most findings have suggested that hyperuricemia is a risk factor for stroke and poor outcome after stroke.Very few studies have suggested that hyperuricemia has protective effect on acute ischemic stroke.Although the effect of hyperuricemia on pathophysiological mechanisms of stroke remains uncertain, the interventional strategy by lowering the levels of blood uric acid for preventing ischemic stroke is worth to be considered. This article mainly reviews the recent clinical trials data and related mechanisms.

OBJECTIVE:To determine the content of epirubicin in human plasma by RP-HPLC.METHODS:The chromatographic separation was performed on YWG C18 column.The mobile phase consisted of water-acetonitrile(12∶7,with pH adjusted to 3.90?0.05 using 85% phosophoric acid)at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 254 nm.RESULTS:The linear range of epirubicin was 0.12～4.80 mg?L-1(r=0.998 4)with the lowest detective range of 0.01 mg?L-1.The intraday RSD was less than 4.40% and the inter-day RSD was less than 5.60%,and the average recovery rate was 99.40%(RSD=4.02%).The sample solution was stable within 12 h under room temperature.CONCLUSION:The method is easy,precise and reproducible to operate and the result of the determination is accurate.