The PTEN, having a dual specificity phosphatase activity, is the first tumor suppressor gene that possess phosphatase activity hitherto. Many researches have suggested that PTEN play a major role in the tumorgenesis. In clinical, the head and neck squamous cell carcinoma(HNSCC) is one of the most common malignant tumors. In this review, advances in the research of PTEN and the relationship between the PTEN and HNSCC are discussed.

OBJECTIVE:To study the inhibitory effects and mechanism of lutein on nasopharyngeal carcinoma C666-1 cells. METHODS:C666-1 cells were stimulated by lutein at different concentrations [0(blank control),20,40,80,160 mg/L] for dif-ferent time(0,12,24,48 h). The proliferation rate of cells was determined by CCK-8 assay,and apoptotic rate of cells was deter-mined by TUNEL method;Western blot was adopted to determine the phosphorylation of S6K and S6 proteins of AMPK and mTOR pathway. RESULTS:Compared with blank control group,proliferation rate of C666-1 cells was significantly reduced after treated with lutein(80,160 mg/L)for 48 h and lutein(160 mg/L)for 12,24,48 h(P<0.05). After treated with lutein(80,160 mg/L)for 48 h and lutein(160 mg/L)for 24,48 h,cell apoptosis was significantly increased(P<0.05). Lutein(80,160 mg/L) could promote intracellular AMPK phosphorylation,and inhibits mTOR pathway S6K,S6 protein phosphorylation after 48 h treat-ment (P<0.05). CONCLUSIONS:Lutein can inhibit nasopharyngeal carcinoma C666-1 cell proliferation and induce nasopharyn-geal carcinoma cell apoptosis and inhibit S6K,S6 protein phosphorylation through promoting AMPK phosphorylation.

AIM: To examine the expression and distribution of tumor necrosis factor-? (TNF-?), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-?, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-? expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion ( P

AIM: To study the influence of basic fibroblast growth factor(bFGF) on treating ed palatal perforation in rats. METHODS: bFGF was given to the early palatal perforation in rat. The granulation tissues in perforations were grossly and pathologically obserVed. RESULTS: The the of wound healing was significantly in- crease in the bFGF group (P

AIM: To study the expression of mic2/CD99 protein and their correlation with Eber-1/LMP-1 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma. METHODS: Immunohistochemical staining, in situ hybrization and tissue microarry technique were used to detect the expressions of mic2/CD99 and Eber-1/LMP-1 of H/RS cells in 43 cases of cHL and 16 cases of NHL. RESULTS: The positive rate of CD99 protein expression in 43 cases of cHL was 2.3% (1/43) , mic2 was 55.8% (24/43), LMP1 was 58.1% (25/43) and Eber-1 was 53.5% (23/43). The expressions of CD99 and mic2 in the NHL group were higher than those in cHL group (P0.05). There was a negative correlation between the expression of CD99 protein and LMP1 in H/RS cells (P0.05). There was a significant correlation between the high expression of LMP1 and a low expression of CD99 in the young patients (P0.05). CONCLUSION: There is a negative correlation between the expression of LMP1 and CD99 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma.

Objective To study the effect of designing and application of portable thermometer transceiver and its application. Methods A portable thermometer transceiver was designed and used to measure the temperature of 31,386 patients. Meanwhile, the traditional temperature measurement method was used among 31,378 patients. The two groups were compared in view of time for assigning and collecting the meter. Results The time for assigning the portable thermometer transceiver and receiver was (0.08 ±0.03) min, while that for the traditional measurement method was (1.06 ±0.03) min. There was statistically significant difference between the groups (t=29.231, P<0.001). Conclusion The portable thermometers for temperature measurement can save time and improve the efficiency of nursing staff.

Objectives To develop a rapid genotyping method of clinical isolates of Malassezia from patients with tinea versicolor by PCR-RFLP,and to evaluate reliability of the approach as compared with biochemical classification.Methods Tween assimilation test and catalase reaction were carried out to identify 74 isolates of Malassezia species from patients with tinea versicolor and 7 Malassezia reference strains.The sequence of 28S rDNA of Malassezia species was amplified by PCR,and then the product was analyzed by RFLP with Eco88I,Bsp143Ⅱ and BshNⅠ,respectively.Results M.restricta,M.obtusa and M.pachydermatis were successfully identified by three restriction endonucleases.M.restricta was found to be more diverse from the other 6 species in genetic homology.By comparison with PCR-RFLP technique,a possible mistake was discovered with biochemical method.Conclusion PCR-RFLP is a promising molecular biological technique,which could rapidly and correctly classify Malassezia species.

Objective To explore the efficacy and safety of nasal polypectomy combined with intranasal ethmoidectomy in treating nasal polyp.Methods 80 patients with nasal polyps (ob-servation group)were treated with nasal polypectomy combined with intranasal ethmoidectomy. Therapeutic effect,the postoperative complications and 1 year relapse were observed and compared with the results reported by HUANG Yuanhong,LI Feng,LI Weiping.Results Cure rate (75%)in the observation group was significantly higher than HUANG Yuanhong group,LI Weip-ing group and LI Feng group (χ2=4.365,4.342,4.365,P =0.034,0.033,0.028,P <0.05), while total effective rate (97.5%)was significantly higher than LI Feng group (χ2=3.846,P =0.038 <0.05.Incidence rate of complications (2.5%)in the observation group was significantly lower than HUANG Yuanhong group,LI Weiping group and LI Feng group (χ2=5.365,9.126, 5.365,P =0.034,0.012,0.006,<0.05),and the recurrence rate (1.25%)was significantly lower than HUANG Yuanhong group and LI Weiping group (χ2=18.365,24.321,P <0.01). Conclusion Nasal polypectomy combined with intranasal ethmoidectomy can reduce the complica-tions,alleviate postoperative pain,prevent postoperative recurrence,and its clinical efficacy is bet-ter.

Objective To explore the efficacy and safety of nasal polypectomy combined with intranasal ethmoidectomy in treating nasal polyp.Methods 80 patients with nasal polyps (ob-servation group)were treated with nasal polypectomy combined with intranasal ethmoidectomy. Therapeutic effect,the postoperative complications and 1 year relapse were observed and compared with the results reported by HUANG Yuanhong,LI Feng,LI Weiping.Results Cure rate (75%)in the observation group was significantly higher than HUANG Yuanhong group,LI Weip-ing group and LI Feng group (χ2=4.365,4.342,4.365,P =0.034,0.033,0.028,P <0.05), while total effective rate (97.5%)was significantly higher than LI Feng group (χ2=3.846,P =0.038 <0.05.Incidence rate of complications (2.5%)in the observation group was significantly lower than HUANG Yuanhong group,LI Weiping group and LI Feng group (χ2=5.365,9.126, 5.365,P =0.034,0.012,0.006,<0.05),and the recurrence rate (1.25%)was significantly lower than HUANG Yuanhong group and LI Weiping group (χ2=18.365,24.321,P <0.01). Conclusion Nasal polypectomy combined with intranasal ethmoidectomy can reduce the complica-tions,alleviate postoperative pain,prevent postoperative recurrence,and its clinical efficacy is bet-ter.

Objective To explore the factors that influence the quality of life of perimenopausal women which take the stress model as the theoretical framework. Methods A total of 747 perimenopausal women were investigated with the general questionnaire, SF-12, self-efficacy questionnaire, Eysenck personality questionnaire short scale for Chinese (EPQ-RSC), the simplified coping style questionnaire, and the social support rating scale. After surveying, the supposition model was confirmed and revised by path analysis. Results The quality of life perimenopausal women was (56.72±8.47), which was lower than the score of women without perimenopausal syndrome ( 71. 24 ± 5. 66 ) ( P<0. 05 ) . The correlation analysis and structural equation analysis showed that negative coping, utilization of social support, personality characteristics of outgoing and introvert people and degree of perimenopausal syndrome all can directly influence the quality of life ( P<0. 0. 5 ) , and also can indirectly influence the quality of life though self-efficiency. Self-efficiency is the most important predictor variable of quality of life in perimenopausal women.Conclusions Perimenopausal women′s quality of life needs to be improved. Perimenopausal syndrome, self-efficacy, negative coping, utilization of social support and personality characteristics of outgoing and introvert people play an important role in the quality of life of perimenopausal women.