1.Study on the improvement of ADL in post-stroke patients:a preliminary report
Chinese Journal of Rehabilitation Theory and Practice 2000;6(4):156-158
To analysis effects of rehabilitation and improvement of ADL in post-stroke patients, 114 cases were divided into two groups A and B. Rehabilitation treatment started for the patients in group A in two or three months after onset of the stroke, while group B in four or six months. The ADL of 114 cases were evaluated before rehabilitation and one month after rehabilitation. Treatment was siginificant in both groups (P<0.001). The improvement of ADL in group A was better than group B in grooming, feeding, dressing, toilet, utensil using, moving on the bed, locomotion and walking (P<0.05). Rehabilitation treatment is favorable for improving ADL ability of post-stroke patients. The earlier rehabilitation treatment starts, the better improvement of ADL is.
2.Inhibitory Effects and Mechanism of Lutein on Nasopharyngeal Carcinoma C666-1 Cells
Min SHEN ; Hui LIU ; Lijia WAN
China Pharmacy 2016;27(1):53-55
OBJECTIVE:To study the inhibitory effects and mechanism of lutein on nasopharyngeal carcinoma C666-1 cells. METHODS:C666-1 cells were stimulated by lutein at different concentrations [0(blank control),20,40,80,160 mg/L] for dif-ferent time(0,12,24,48 h). The proliferation rate of cells was determined by CCK-8 assay,and apoptotic rate of cells was deter-mined by TUNEL method;Western blot was adopted to determine the phosphorylation of S6K and S6 proteins of AMPK and mTOR pathway. RESULTS:Compared with blank control group,proliferation rate of C666-1 cells was significantly reduced after treated with lutein(80,160 mg/L)for 48 h and lutein(160 mg/L)for 12,24,48 h(P<0.05). After treated with lutein(80,160 mg/L)for 48 h and lutein(160 mg/L)for 24,48 h,cell apoptosis was significantly increased(P<0.05). Lutein(80,160 mg/L) could promote intracellular AMPK phosphorylation,and inhibits mTOR pathway S6K,S6 protein phosphorylation after 48 h treat-ment (P<0.05). CONCLUSIONS:Lutein can inhibit nasopharyngeal carcinoma C666-1 cell proliferation and induce nasopharyn-geal carcinoma cell apoptosis and inhibit S6K,S6 protein phosphorylation through promoting AMPK phosphorylation.
3.Chemical Constituents in Tibetan Medicine Dolomiaea Souliei (Franch.) Shih
Hua WEI ; Lingling LIU ; Lijia XU ; Yong PENG ; Peigen XIAO
China Pharmacist 2017;20(5):785-787
Objective: To study the chemical constituents in the dry roots of Dolomiaea souliei (Franch.) Shih.Methods: Various chromatographic methods were used to isolate and purify the chemical constituents of Dolomiaea souliei, and the structures were elucidated through the analysis of spectral data and literatures.Results: Six compounds including 3 sesquiterpene compounds and 3 fatty acids were obtained and identified as dihydrodehydrocostuslactone(Ⅰ), vladimenal(Ⅱ), arbusculin A(Ⅲ), n-hendecane(Ⅳ), butanedioic acid(Ⅴ) and methyl linoleate(Ⅵ).Conclusion: Compounds Ⅳ-Ⅵ are obtained from the genus of Dolomiaea for the first time.
4.Analysis on the Invention Patent of Traditional Medicines about Type-2 Diabetes Mellitus
Wenting WAN ; Yunyun MA ; Lijia XU ; Zhiyi SUN ; Haibo LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(6):1240-1245
In this paper, the patent of traditional medicines of type-2 diabetes mellitus before 2014 were searched, totally 1 229 patent family information were obtained. The patent information including developing trends, geographic distribution, competitive agencies, technical focus, herbs preferences and so on were analyzed. China is in a leader position on the number of applying patent of using traditional medicine to treat type-2 diabetes mellitus, and the main applications were research institutes, universities and pharmaceutical enterprises. Although the views and theo-ries on treatment of type-2 diabetes mellitus are variety and abound, the most frequently used traditional medicines are tonics and heat-clearing. This article reflected the history, clinical treatment way, developments, application of the diabetes traditional drugs after a meta-analysis,efforts to guide a better understanding and further research.
5.Therapeutic effect evaluation of oral rehydration salts [Ⅰ] on autonomic nerve mediated syncope in children with different hemodynamic patterns
Xiaoyan LIU ; Cheng WANG ; Lijia WU ; Ping LIN ; Fang LI
Chinese Journal of Applied Clinical Pediatrics 2017;32(1):26-29
Objective To explore the effects of oral rehydration salts [Ⅰ] (ORS Ⅰ) for autonomic nerve mediated syncope(NMS) in children with different hemodynamic patterns.Methods A total of 105 patients with unexplained syncope and prodromal symptoms of syncope who were confirmed as NMS by head-up tilt table test(HUTY) and treated in the Department of Pediatric Cardiovasology,Children's Medical Center,the Second Xiangya Hospital,Central South University,from March 2012 to February 2015.Their ages were from 4 to 18 years old,the average age was (11.96 ± 2.86) years old.Totally 73 cases were diagnosed as vasovagal syncope (VVS) (46 cases were vasodepressor type,27 cases were VVS mixed type or cardioinhibitory type),while 32 cases were diagnosed as postural orthostatic tachycardia syndrome(POTS).Simple random method was used to divide them into conventional therapy (health education and tilt training) plus ORS Ⅰ group (55 cases),and conventional therapy group (50 cases).Patients were followed up for 6-25 (14.82 ± 6.13) months.The recurrence of syncope and review of HUTT outcome assessment in 6 months,treatment was studied to evaluate short-term efficacy of 2 different therapies for NMS in children with different hemodynamic patterns.Taking recurrence of syncope as outcome events,Kaplan-Meier curves were drawn to compare long-term efficacy of different therapies in treating NMS children.Results There was no statistical difference in the short-term efficacy among the different hemodynamic patterns when treated with conventional therapy plus ORS I,or conventional therapy(all P > 0.05).The cumulative efficiency of the conventional therapy plus ORS Ⅰ was superior to that of the conventional therapy for NMS children through the long-term follow-up study (74.5% vs.52.0%,x2 =14.424,P < 0.01).Patients with vasodepressor patterns had a better response than those with mixed or cardioinhibitory patterns to conventional therapy plus ORS I (90.0% vs.61.1%,x2 =4.435,P < 0.05).Conclusions Compared with VVS mixed type or cardioinhibitory type,children with VVS vasodepressor patterns are more appropriate to take ORS I as initial treatment.
7.Research on the clinical value of urinary NTx,serum BAP and BMD in patients with bone metastasis from malignant tumors
Lingyun WANG ; Xiaofang LIU ; Tao DU ; Lijia LI ; Xiaoyan HU ; Lei LIU ; Ming LI
Chongqing Medicine 2014;(9):1061-1062,1065
Objective To evaluate the variance and clinical significance of urinary pyridinoline cross-linked N-telopeptides of Type Ⅰ collagen(uNTx) ,serum bone specific alkaline phosphates(sBAP) and Bone mineral density(BMD) in patients with bone metastasis malignant tumors .Methods The levels of 53 case patients′,who with bone metastasis ,uNTx and sBAP were measured by ELISA and BMD was detected by dual energy x-ray absorptiometer .40 cases of non-bone metastasis people was assayed .Results The levels of all bone markers in patients with bone metastasis was significantly higher than in the patients without bone metas-tasis(P<0 .05) .The levels of the two biomarkers showed a positive correlation with the number of metastatic loci in bone (P<0 .05) .But there was not significantly different between the levels of uNTx or sBAP with the severity of the bone pain (P>0 .05) . BMD decreased in patients with bone metastasis group ,but it was no difference in the non-bone metastasis(P>0 .05) .Conclusion uNTx and sBAP are helpful for early diagnosis and prevention of patients with bone metastasis from malignant tumors .Patients with malignant gumor is often accompanied by a decrease in BMD .
8.Study on antisense phosphorothioate oligodeoxynudeotide resistance to influenza A virus infection in vitro
Xiaoshan SHAO ; Yuanyuan XIE ; Xiqiang YANG ; Xiaodong ZHAO ; Yao ZHAO ; Wei LIU ; Lijia WANG
Chinese Journal of Microbiology and Immunology 2009;29(2):130-136
Objective To investigate the antiviral effects of antisense phosphorothioate oligodeoxynucleotide (ASODN)in 9HTEO infected with influenza A virus (IFAV) in vitro. Methods The ASODN which complemented to genomic PB1, M2, NS, PB2, HA mRNA of IFAV was used to investigate antiviral effection in vitro. The cytopathic effect (CPE) was observed, and the cell survival rates were measured by MTF assay, plaque assay, RT-PCR, Western blot and Immunofluorescence were performed to test anti-viral efficiency of PB1, M2, NS in cells mRNA and protein level. Results 9HTEO cells infected with IFAV almost all died when the multipicity of infection (MOI) is aboved after 5 days cell culture. The ASODN could increase the cell survival rates. The IFAV PB1, M2, NS significantly reduced CPE of IFAV infected 9HTEO cells, reduced the viral replication of IFAV in the cells (P <0.05). Conclusion The ASODN which targeted the mRNA of IFAV gene showed a significant and specific anti-IFAV effect both in mRNA and protein level in cells culture system. The study indicates that the PB1, M2 and NS mRNA may play an important role in regulating IFAV replication, and ASODN may have inhibitory activity on IFAV replication. The results established the basis for further study on new drugs against IFAV infection include the highly pathogenic H5N1 influenza virus.
9.The role of Huang-qi in balance of THl/TH2 in asthma on dendritic cells level
Yan CHEN ; Zhou FU ; Kunhua CHEN ; Xiqiang YANG ; Enmei LIU ; Lijia WANG ; Xin LI
Chinese Journal of Microbiology and Immunology 2008;28(12):1064-1069
Objective To investigate the role of Huang-qi in balance of TH1/TH2 in asthma on dendritic cells level. Methods DCs from human peripheral blood mononuclear cells(PBMC) were induced by rhGM-CSF and rhIL-4, and then were identified. The level of IL-12 and IL-10 produced by DCs were de-tected by ELISA assay. After autoreactive T cells, mRNA of T-bet and GATA-3 was measured by RT-PCR. Simultaneously, IL-4 and IFN-γ were determined by flow cytometer. Results After 7 days culture, IL-12 was significantly decreased in asthma group compared to control group (P < 0.01), whereas IL-10 on the opposite. At the 7th day of co-culture with T cells derived from floating cells, the IFN-γ/and T-bet mRNA level in asthma group were significantly decreased than that in control group, whereas IL-4, GATA-3 mRNA level, the ratio of IL-4/IFN-γ and GATA-3/T-bet were apparently increased in asthma group than that in control group(P<0.01). After Huang-qi treatment, the IFN-γ/and T-bet mRNA level were significantly in-creased, whereas the ratio of IL-4/IFN-γ and GATA-3/T-bet, and the IL-10 level were apparently de-creased, but the level of IL-12, IL-4 and GATA-3 mRNA were not changed significantly. Conclusion DCs in asthma regulated the balance of TH1/TH2 by means of secreting decreased IL-12 and increased IL-10, that made TH2 playing a dominance role which is the key factor in initiating asthma. Huang-qi regulated DCs through decreasing the level of IL-10, and thus decreased the ability of inhibiting the differentiation of TH1 from TH0, that is also inhibiting the differentiation of TH2 from TH0 directly.
10.Influence of IL-17 on mesangial cell and its molecular mechanism
Li WANG ; Qiu LI ; Cuicui LI ; Haiping YANG ; Lijia WANG ; Wei LIU
Journal of Clinical Pediatrics 2014;(2):178-182
Objectives To investigate the effect of IL-17 on proliferation and secretion of IL-1βin the human mesangial cell line (HMCL) and explore the molecular mechanism. Methods The proliferation of HMCL induced by IL-17 was detected by MTT, the secretion of IL-1βin HMCL was measured by ELISA and the expression of NF-κB was detected by immunocyto-chemistry. Results IL-17 did not impact HMCL proliferation (P>0.05) but dose-and time-dependently promoted the secretion of IL-1β(P<0.05). And the expression of NF-κB induced by IL-17 increased comparing to that in the control group (P<0.05). Con-clusions IL-17 may induce HMCL to secrete IL-1βvia NF-κB pathway that results in kidney injury.