Objective To achieve the DICOM3.0-based management system for medical image and data. Methods Under the context of Microsoft Windows XP, Microsoft Visual C++.NET, LeadTools V14.5 development SDK and Microsoft SQL Server 2000 were used to actualize a medical image data management system based on DICOM 3.0 standard. Results This system could store and read the DICOM 3.0 standard -based medical image correctly and establish the corresponding control of authority to different users. Conclusion This system is easy for maintenance and operation with convenient search function.

OBJECTIVE To cultivate central supply unit member's custom of watching detail and dealing with everything carefully. To enhance central supply unit work's quality and efficiency,and decrease the phenomenon of article sterilized overdue and nipping tool's scathe. METHODS To organize study and enhance the cognition. To constitute regulations,systems and operation rule. To investigate the condition of medical instrument utilized,and pay attention to detail service for packaging sterilization. RESULTS The rate of overdue sterilized article decreased from 0.008% to 0.001%. The number of nipping tool's injuries decreased from 18 in 24 to 2 in 22 when the detail management was actualized. The rate of injury decreased from 75.0% to 9.09%. CONCLUSIONS In order to ensure the quality of asepsis of articles,we need to alter our conceptions,strengthen our responsibility,servicing more initiatively and carefulness.

Protein separation method is the core factor of the proteomics strategy. There are three methods for the protein separation, include two dimensional gel electrophoresis, liquid-phase electrophoresis and Mass Spectrometry, and protein-chip. In this article, the principles, processos, and development on the three separation protein methods.were reviewed.

Objective To explore the related factors of senile acute pulmonary thromboembolism(PTE) patients,and to observe the prognosis and analyze the strategy of control and prevention to reduce the incidence. Methods 50 elderly patients with acute PTE were selected,related factors were analyzed by using the retrospective investigation,and the prevention and control were administrated.Results The mainly disease occurred of the elderly patients with acute PTE were combined with chronic diseases 42% (21 /50),with lower extremity DVT 26.00%(13 /50)and with recent trauma,fracture and operation 20%(10 /50).Elderly patients with acute PTE who were ≥70 years of age and lying in bed the time≥1 month,were 58%(29 /50)and 62%(31 /50),which were higher than those of the other age <70 years old and lying in bed time <1 month of patients,the difference was statistically signifi-cant(χ2 =9.26,9.80,all P <0.05).Conclusion The related risk factors for elderly patients with acute PTE mainly correlate with age,lying in bed time,chronic diseases,patients with lower extremity DVT and recent trauma,fracture, operation,the variety of factors and overlap each other.Mastering the variety factors of PTE in the nursing process,and taking effective measures to prevent and control the factorscan reduce or avoid the occurrence of acute PTE,and im-prove the prognosis of the patients.

Current curriculum provision and teaching patterns of computer science in medical school has been far behind the requirements of clinical and research works, which restricted the develop-ment of medical talents to a large extent. According to the experiences of teaching practice,Department of Computer Science in Chongqing Medical University launched reform on medical computer science from aspects of teaching practice,curriculum provision,teaching methods and evaluation methods. Computer courses were opened according to students' computer knowledge and the profession. Task-based teaching and comprehensive evaluation methods were employed in order to improve the teaching quality.

Objective:To observe the sedation effects of midazolam on maxillofacial postoperative patients with nasotracheal intubation. Methods:40 cases were equally and randomly divided into two groups: sufentanil group (group S) and sufentanil-midazolam group(group S-M).The continuous dose was 2 ml/h. If the patients feel uncomfortable, patient controlled intravenous analgesia (PCIA) was used. To observe the changes of circulation and respiration,the changes of visual analogue scale(VAS) and Ramsay score, the patients' bucking times within 24 h and the PCIA times, patients' total satisfactory degree and the complications during postoperative analgesia were recorded. Results:There were significant decreases in VAS of two groups after postoperative analgesia 1, 4, 12, 24, 48 hours(P

OBJECTIVE To study the lower respiratory tract infection caused by Stenotrophomonas maltophilia and the resistance situation. METHODS The clinical data of 79 cases of lower respiratory tract infection caused by(S.maltophilia) were analyzed and the susceptibility test was made using ATBPSE5 kits. RESULTS All patients had clinical symptoms,and the rate of empirical using of broad-spectrum antibiotics reached to 100%.In all these patients,74.68% were older than 60,and 51.90% received invasive operation such as trachea intubation or incision,and the immmunosystem function of 62.03% was compromised.All these were high risk factors resulting in the lower respiratory S.maltophilia infection.All cases showed resistance to most broad-spectrum antibiotics except ticarcillin/clavulanic acid and sulfamethoxazole/trimethoprim(Cotrimoxazole).The latter two antibiotics still showed high sensitivity to the pathogen. CONCLUSIONS It is necessary to avoid the risk factors causing nosocomial(S.maltophilia) infection.Ticarcillin/clavulanic acid and Cotrimoxazole are the first choice for clinical treatment.Using fluoroquinolone and ceftazidime simultaneously can improve the curing rate.

The experiment was conducted at the Basic Laboratory of Integrated Traditional Chinese and Western Medicine, Chengdu University of TCM from March 2005 to March 2006. A total of 40 BALB/C male mice aged 6-8 weeks were selected, and randomly assigned into 4 groups: bcl-2 control group, bcl-2 experimental group, cmy-c control group and cmy-c experimental group with 10 in each group. From the first day of experiment, all the mice were treated with 5 g/L cyclophosphamide solution by intraperitoneal injection for successively 5 days, 6 mL/kg per day. From days 11 to 12, the mice in the bcl-2 experimental group and cmy-c experimental group received Danggui buxue tang by gastric perfusion, 6 mL/kg per day for 10 days. The mice in the control group received saline of the same volume. At day 20, all the mice were killed to obtain bone marrow cells of one side femoral bone of mice. The smear received bcl-2 in situ hybridization immunohistochemical staining after centrifugalization and washing in the bcl-2 control and bcl-2 experimental group. The smear received cmy-c in situ hybridization immunohistochemical staining after centrifugalization and washing in the cmy-c control and cmy-c experimental group. Five fields of each section were red into image analyzer. The sum of positive cell area and the sum of integral absorbance in each field were analyzed. The results showed that the sum of positive cell area was (16.11?3.01)?102 ?m2, and the sum of integral absorbance was (19.9?2.42)?105 in the bcl-2 experimental group. Compared with the bcl-2 control group, there was significant difference (P

ObjectiveTo evaluate the result of problem-based learning combined with Casebased learning teaching model in the clinical probation of digestive medicine.MethodsPBL combined CBL teaching method and traditional teaching method were used respectively among 120 medical students who were divided into two groups randomly. Educational effects were evaluated through examination and ques-tionnaire.Results The PBL combined CBL group test scores are significantly higher than traditional teaching group ( P＜0.05 ).96.4％ students thought it was necessary to have PBL combined CBL teaching.ConclusionsPBL combined CBL teaching method is an effective teaching method for medical students.

BACKGROUND: Chitosan and sodium alginate are the good natural materials for microcapsule, and also used widely in tissue engineering. Our research teams have made thorough work at anticoagulant materials, but these materials are inert or simulate the liquid crYstal form of blood vessel wall. While in this experiment, on the base of our previous study, we microencapsulated heparin with biotic anticoagulation activity and other specific performances in order to enable microcapsule to have a long time releasing effect of medicine.OBJECTIVE: To microencapsulate the low molecular heparin so as to ensure the stability of heparin in vivo and analyze the effect of content of chitosan on the performance of heparin microcapsules basing on the natural chitosan and sodium alginate as the enwrapped materials of microcapsules.DESIGN: Open experiment.SETTING: Department of Material Science and Engineering, Jinan University.MATERIALS: The experiment was performed at the laboratory of Department of Material Science and Engineering, Jinan University from October 2004 to June 2005. Heparin, with relative molecular mass＜ 5 000, was provided by Shandong Freda Biochem Co., Ltd.,; Chitosan was provided by Shanghai Bio Life Science & Technology Co., Ltd, DD≥90%, η＜ 100 cps;Sodium alginate was provided by Qingdao Bright Moon Seaweed Industrial Co., Ltd. Emulsions were Span80, and CaCl2, which were both made in China.METHODS: ①Preparation of heparin/chitosan microcapsules (HCM):Some heparin aqueous solution was emulsified in liquid paraffin. The reaction system was stirred fully and presented emulsion. Then the whole reaction system was warmed to be at 50 ℃ and maintained for 20 minutes. Afterwards, 20 g/L chitosan solution was added slowly, subsequently with raising the temperature to be at 60 ℃ and then glutaraldehyde was dropwised keeping the reaction system at 80 ℃ for 1hour. Centrifugation, filtration and washing followed by washing with kerosene fully, remain organic was extracted by dehydrated alcohol with extractor were performed.Drying and xeransis in vacuum were done at last. ② Preparation of heparin-sodium alginate-chitosan microcapsules (HSCM) :Heparin aqueous solution and sodium alginate were emulsified in paraffin, and the reaction system was stirred into emulsion at room temperature for 20 minutes, then 3% CaCl2 solution containing different concentrations of chitosan was added slowly. 30 minutes later, Microcapsules were separated, washed and dried as the treatments as before. ③ Drug content and envelope efficiency were measured, heparin standard curve was determined and in vitro releasing effect of heparin microcapsules was also measured.MAIN OUTCOME MEASURES: ①Effect of chitosan solution concentration on preparation of heparin-chitosan microcapsules; ② Effect of glutaraldehyde dosage on preparation of heparin-chitosan microcapsules; ③Effect of sodium alginate concentration on hepatin-sodium alginate; ④Effect of chitosan concentration on hepatin-sodium alginate-chitosan microcapsules. ⑤ In vitro release of heparin microcapsules enwrapped by different materials. ⑥Measurement of heparin content and envelope efficiency. ⑦ Observation of heparin microcapsule under scanning electron microscope RESULTS: ①With the increasing concentration of chitosan, the color of production changed from yellow to dark, and microcapsules were increscent, but the microcapsules uniformity and property of balling were increased. ②The increasing content of glutaraldehyde led darker production.Increase of glutaraldehyde content made production bond each other severely. The glutaraldehyde, which did not react with chitosan, can solidify itself and presented anomalous microcapsules forming. ③There was not obvious balling property of the production with the change of concentration of sodium alginate. ④The balling property of microsphere was good with increasing concentration of chitosan. However, microcapsules conglutinated with each other. 2% chitosan would be better. ⑤With the increase of chitosan content, the releasing speed ofheparin became slow. ⑥The envelope efficiency was about 58% when microcapsule contained 20%(wt) of chitosan, and used chitosan only the envelope efficiency could approach to 79.9%. ⑦ The surface of microcapsules with chitosan was very compact,and with increasing of content of glutaraldehyde, microcapsules would bond each other.CONCLUSION: Chitosan at certain concentration will affect the uniformity and balling property of microcapsules. Chitosan dosage can alter the envelope efficiency of heparin. Envelope efficiency of heparin is increased and releasing speed of heparin is decreased with the increase of content of chitosan.