Objective To develop an improved enzymatic method for assaying creatinine in serum and urine. Methods Cratinine iminhydrolase catalyzes the conversion of creatinine to N-methylhydantoin and ammonia,the later combines with 2-oxoglutarate and NADPH in the presence of glutamate dehydrogenase.Before beginning the reaction sequence,endogenous ammonia is removed by an “ammonia scavenger system”that involves the same auxiliary reaction.The NADPH and 2-Oxoglutarate consumed are restored through a reaction catalyzed by isocitrate dehydrogenase.The activity of this strictly magnesium-dependent enzyme is completely blocked by magnesium-complexing reagent that acts promptly whenever the reaction has started with creatinine iminohydrolase as startor.The reaction is monitored kinetically by measuring the NADPH decrease via its absorbance at 340nm.Results The method affords a simple,rapid,and sensitive assay with good precision and extended linearity.Test results compare closely with the HPLC precedure.The proposed method is not subject to interference from several metablites or from the drugs used commonly in clinics.Conclusion The method is easily automated and is suitable for routine work in clinical laboratories.

AIM:To detect the effect and underlying mechanism of parthenolide ( PN) on neointimal hyperpla-sia.METHODS:After 1 week of high-fat feeding, 30 male New Zealand white rabbits (2.0~2.3 kg) were randomly di-vided into 6 groups: sham +NS, rabbits received 0.9% normal saline after sham operation; sham +DMSO, rabbits re-ceived DMSO after sham operation;balloon injury(BI)+NS, rabbits received NS after balloon injury;BI+DMSO, rabbits received DMSO after balloon injury;BI+PN low, rabbits received PN at 1 mg/kg after balloon injury;BI+PN high, rab-bits received PN at 2 mg/kg after balloon injury .The drugs were intraperitoneal injected once a day after the operation until sacrifice.After fed with high-fat diet for 4 weeks, the intima-media thickness, the expression of caspase-1, IL-1β, the lev-els of IL-8, TC, TG, LDL and HDL in the serum were measured .RESULTS:Compared with sham +DMSO group, the thickness of intima, the amount of caspase-1, IL-1βand IL-8 in BI +DMSO group were significantly increased ( P <0.05).The levels of caspase-1, IL-1βand IL-8 were significantly decreased in BI +PN high group compared with BI +DMSO group (P<0.05).CONCLUSION:Neointimal hyperplasia is suppressed by PN after balloon injury , the potential mechanism may be associated with its anti-inflammatory role .

Objective To study the expression of KAI1mRNA in cervical carcinoma and its clinical significance.Methods The transcription-polymerase chain reaction(RT-PCR) method was applied to detect the expression of KAI1mRNA in 53 fresh freezing cervical tissues.The findings were compared with the clinicopathological data.Results The expression of KAI1mRNA was (0.309?0.093) in normal cervix,(1.608?0.289) in cervical intraepithelial neoplasia(CIN),(0.948?0.910) in cervical carcinoma respectively.The relative densitometric value of KAI1mRNA decreased in late stage of cervical cancer in local cervical cancer with lymphatic metastasis(P

Objective To study the influence of different positions in the isokinetic muscle test of knees by CON-TREX Biomechanical Test and Training System, so as to select the suitable conditions for forensic identification of muscle strength test. Methods Fifty-two healthy volunteers joined the isokinetic muscle strength test in unfixed and fixed position, respectively and in two kinds of angular speed (60°/s and 30°/s). The differences of peak torque (PT) and peak torque angle (PTA) between bilateral knee flexor and extensor were statistically analyzed. Results In the unfixed position, under the two speed, there was statistically significant difference in PT between bilateral knee flexor and extensor (P<0.05);while in the fixed position, under the two speed, there was no statistically significant difference (P>0.05). In any kind of conditions, the PTA of bilateral knee flexor and extensor did not have statistically signifi-cant difference(P>0.05). Conclusion The position of the subject influences the results of PT. So the po-sition of subject in knees isokinetic muscle test should be regulated.

Objective To explore the effect of bilateral scalp acupuncture combined with synchronous motorrehabilitation training on acute cerebral infarction patients with movement dysfunction. Methods Eighty acutecerebral infarction patients were evenly randomized into treatment group and control group. Both groups weregiven routing medicine for neurology and body acupuncture. Additionally, the treatment group received bilateralscalp acupuncture combined with synchronous motor rehabilitation training, and the control group receivedaffected-side scalp acupuncture combined with synchronous motor rehabilitation training. The acupuncture andtraining were carried out once a day. One course of treatment consisted of six continuous treatment days and onerest day, and the treatment lasted 4 courses. Before and after treatment, the scores of Fugl-Meyer assessment( FMA) for motor function, the modified Barthel index (MBI) scores and the neurological deficit scores(NDS) were observed for the evaluation of therapeutic effect. Results After treatment, FMA scores and MBIscores were increased, and NDS were decreased in both groups compared with those before treatment, thedifferences being significant (P<0.05) . On treatment day 14, the differences of the observation indexes wereinsignificant between the two groups (P>0.05) . On treatment day 28, FMA scores and NDS of the treatmentgroup were superior to those of the control group ( P <0.05) , but the differences of MBI scores wereinsignificant between the two groups ( P >0.05). Conclusion Bilateral scalp acupuncture combined withsynchronous motor rehabilitation training exerts remarkable effect on improving motor function of acute cerebralinfarction patients with movement dysfunction.

Objective:To discuss the changes of peripheral blood T Lymphocyte subsets in patients with chronic HBV infection to know the relation between T Lymphocyte subsets and HBV replication and the relationship between T Lymphocyte subsets and activity of liver cirrhosis.Methods:Peripheral blood lymphocytes were analysed by flow cytometry in 99 patients with chronic HBV infection.Results:The T Lymphocyte subsets counts were marked higher in patients with chronic active hepaitis B than those in patients with liver cirrhosis.CD4+/CD8+ ratios were significantly higher in active cirrhosis than those in non-active cirrhosis(P

Aim The present study was designed to investigate the growth inhibition and anti-angiogenesis of arsenic trioxide (As 2O 3) on B16 cells xenografts in C57BL/6J mice and observe the effects of As 2O 3 on cell proliferation, cell morphology, cell cycle and apoptosis in vitro. Methods Mice melanoma cell line B16 was transplanted into C57BL/6J mice. The weight of tumor and percent of tumor development were examined after As 2O 3 intraperitoneal administration. HE staining and immunohistochemistry for Ⅷ-R Ag were performed to detect the microvessel density in tumor tissues. CellTiter 96 Aqueous One was used to determine the cell proliferation. Giemsa and Feulgen staining were used to observe the morphological changes of the cells. Cell cycle and apoptosis were analyzed by flow cytometry (FCM). Results As 2O 3 significantly inhibited the tumor growth in vivo, with an inhibitory rate of 81.61%. The microvessel density in tumor tissues was obviously reduced after treated with As 2O 3. There was obviously a concentration-dependent relationship between As 2O 3 and the inhibition of B16 cells proliferation (P

Objective:To determine the role of lncRNA TUG1 in pancreatic β cells functioning both in vitro and in vivo.Methods:The lncRNA TUG1 expression in mice pancreas,brain,muscle and other different tissues was examined through qRT-PCR.MTT,flow cytometry,GSIS,ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on insulin secretion in vitro and in vivo.Results:lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues.Knockdown of lncRNA TUG1 expression resulted in decreased insulin secretion in β cells both in vitro and in vivo.Immunochemistry analyses showed decreased relative islet area after treatment with lncRNA TUG1 siRNA.Conclusions:Downregulation of lncRNA TUG1 expression can affect insulin secretion in pancreatic β cells in vitro and in vivo,and lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells.

AIM: To observe the neuroprotective effect of combined treatment with taurine and diazepam against focal cerebral ischemia-reperfusion in rats. METHODS: Sixty male Sprague-Dawley rats were randomly divided into five groups: sham-operation group, vehicle group, taurine group (200 mg/kg, ip), diazepam group (10 mg/kg, ip) and combined treatment group (taurine 100 mg/kg+diazepam 5 mg/kg). Focal cerebral ischemia was induced by the method of middle cerebral artery occlusion (MCAO) in rats, and reperfusion was emerged by removing the thread 2 h later. The drugs were administered respectively at the time of reperfusion, and subsequently repeated once 12 h later. The animals in vehicle group were intraperitoneally injected with isodose normal saline. The neurological deficit score, the brain water content and cerebral infarction were measured 48 h after MCAO. Other 5 group animals of focal cerebral ischemia-reperfusion (n=16 in each group) were set up as mentioned above and accepted treatments 10 h after reperfusion, likewise repeated once 12 h later. Twelve animals in each group were adopted the same management as the previous 5 groups at 48 h after MCAO. The remained 4 animals in each group were sacrificed until two weeks after MCAO to observe the histopathological changes by nissl staining. RESULTS: Compared to vehicle group, the animals in combined treatment group at 2 h or 12 h after MCAO both decreased the neurological deficit score, reduced the brain water content and infarct volume (P<0.01 or P<0.05). The combined treatment significantly alleviated the neurological necrosis as well. The neuroprotective effect of the combined treatment was superior to that of using taurine or diazepam alone. CONCLUSION: These results suggest that combination of taurine and diazepam treatment has a coordinate neuroprotective effect on both the acute and chronic brain damage of focal cerebral ischemia-reperfusion.

Objective To investigate the efficacy of bend-assisted laparoseopie fight bemicolectomy for right colon carcinoma in elderly obesity patients. Methods 20 obesity eases undergoing hand-assisted laparoscopic right bemicolectomy were retrospectively reviewed, and compared with 25 obesity cases with transabdominal right hemicolectomy in the same period. The safety, recovery, eradication and stress reaction were compared. Results In hand-assisted laparoscopic group, there were less bleeding loss, rapid recovery to normal temperature and gastrointestinal function,ont of bed activity, short-time hospitalization compared with transabdominal group(P <0.01). There were no differences in operative time, numbers of lymph nodes removed, postoperative complications and length of specimen between two gronps(P > 0.05). CRP, IL-6, adrenocorticotrophic hormone (ACTH) and cortisol were more significantly increased in two groups after operation than before operation (P < 0. 05) , and this increase was moreprominent in transabdominal group than in hand-assisted laparoacopic group (P < 0.05). Conclusion Hand-assis-ted laparoscopic right hemicolectomy is a safe and effective way for elderly obesity patients,especially in postoperative recovery and physical stress reaction.