0.05).Conclusion The curative effect of moxibustion and Shenlingbaizhu pills are definite to AIDS associated diarrhea.The treatment can improve the quality of life of AIDS patients with good safety.

Objective To evaluate the efficacy of flexible laryngeal mask airway and sevoflurane in pediatric strabismus surgery. Methods Totally 60 children undergoing elective strabismus surgery were recruited and divided into two groups with 30 cases each by random digits table:group T, in which children accepted endotracheal tube intubation and combined intravenous and inhalation anesthesia with mechanically ventilation; and group F, in which children adopted both flexible laryngeal mask airway and inhalation anesthesia of sevoflurane with spontaneous breath. Airway peak pressure (Ppeak), average airway pressure (Pmean), blood pressure (Bp) and heart rate (HR) at induction (T1), 1 min (T2), 3 min (T3) and 5 min (T4) after induction, removal of endotracheal tube or laryngeal mask airway (T5), awaking time and extubation time, occurrence of cough, dysphoria and pharyngodynia from being awake to extubation, satisfaction of patients were recorded. Results When compared to baseline, systolic pressure was lower at T, and higher at T2, T3 and T5, and HR was higher at T2-T5 in group T(P< 0.05),Bp was lower at T1-T4 in group F (P < 0.05). Bp and HR at T2-T5 in group F were lower than those in group T (P < 0.05). The awaking time was (8.1 ?2.6) min, the extubation time was (12.6 ?3.2) min, the occurrence of cough and pharyngodynia was 66.7%(20/30), 40.0%( 12/30), the dysphoria (0-1 score) was 7 cases in group T,(5.2 ± 1.1) min, (6.3 ± 1.5) rain,0,6.7%(2/30),25 cases in group F. There were significant differences between two groups (P<0.05). The satisfaction of patients in group F was higher than that in group T, there was significant difference between two groups (P< 0.05). Conclusion Flexible laryngeal mask airway with sevoflurane could offer high efficacy in pediatric strabismus surgery.

Objective To evaluate the effect of multiple-mode analgesia consists of epidural administration of lidocaine,parecoxib intravenously and patient-controlled epidural analgesia (PCEA) on patients after thoracic surgeries. Methods Total 90 patients treated with thoracic surgeries were allocated into 3 groups by random digits table,each with 30 cases. Patients accepted 1% lidocaine 5 ml epidurally 5 min before incision in group A and B,and 5 ml saline as control in group C. When chest closing parecoxib 40 mg were given in group A,and 2 ml saline as control in group B and C. All the patients were given PCEA. Postoperative hemodynamics,dosage of remifentanil and analgetics of PCEA within 48 h, visual analogue scale (VAS) of pain, rescue of pethidine, duration for first pain calling,and satisfactory scores of patients, doctors and nurses were recorded. Results Dosage of remifentanil was lower [(0.72±0.26), (0.84±1.20) μg／(kg·min)], and duration for first pain calling was longer[(35.00±2.78),(12.09±6.27) min] in group A and B than that in group C significantly[(1.80±0.84) μ,g／(kg·min) and (5.95±3.65) min](P<0.05). Analgetics of PCEA with in 48 h in group A was lower than that in group B which was lower than that in group C[(180.40±20.48), (192.54±40.15), (220.26±36.78) ml](P< 0.05). No difference was found on VAS of pain and hemodynamics among three groups when patients were kept immobilized, but VAS of pain in group A and B was lower than that in group C when patients kept moving. Satisfactory scores of patients,doctors and nurses in group A were higher. Conclusion Epidural administration of 1% lidocaine combined with intravenous paiecoxib 40 mg and PCEA an offer satisfactory postoperative analgesia for patients after elective thoracic surgeries.

Objective:To investigate the clinical effect of surgical procedures on patients with chronic sinusi-tis. Method: 104 patients with chronic sinusitis were treated by endoscopic and Caldwell-Luc's approaches. Thelesions were cleared away completely. Result:The clinical cure rate was 96.1％ after 6～12 months (average 9months ) follow up. Conclusion:It suggests that patients with severe maxillary sinus diseases might be cured byendoscopic and Caldwell-Luc's procedure.

OBJECTIVE:To evaluate the clinical efficacy and safety of Chinese herbs Shuxinyin(SXY)in the treatment of angina pectoris in coronary heart disease(CHD).METHODS:This was a double-blind,randomized and multi-center clinical trial.A total of 133 patients with CHD complicated with angina pectoris were assigned to receive Shuxinyin(treatment group)or Shengmaiyin(control group)for 4 wk.The outcome measures were frequency of angina pectoris attacks,symptoms & signs,ECG etc.RESULTS:The total effective rates for angina pectoris in the treatment group was 90.91%,which was significantly higher than in the control group(74.63%)(P0.05).CONCLUSION:SXY is safe and effective in relieving angina pectoris in CHD.

AIM : To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS: Using Diamonsil C_(18) (4.6 mm×250 mm,5 μm) as analytical column.The mobile phase consisted of acetonitrile (A) and 0.1% phosphoric acid ( B ) with gradient elution : 0～18rain,83%～80% B; 18～30 min,80%～86% B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min; Column temperature was at 35 ℃.RESULTS: A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00～48.00 μg/mL( r =0.999 3 ) and 0.64 ～ 40.72 μg/mL( r = 0.999 8 ),respectively.CONCLUSION : The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.

Objective To construct three eukaryotic expression vectors containing wilde-type hGHR gene and its mutants(hGHR-E42K and hGHR-H56R) related to congenital growth hormone insensitivity,then check their expression in CHO cells. Methods With the available PUC-hGHR vector,two mutate hGHR genes (hGHR-E42K and hGHR-H56R) were obtained through mutagenesis. Then three recombinants were cloned into eukaryotic expression vectors pcDNA3.1/zeo(+) with restriction enzymatic reactions. Then with Lipofectamine2000,we transfected expression vectors to CHO cells and screened the stably expressed CHO cells by Zeocin. RT-PCR and/or Western blotting were used to examine hGHR and STAT5-P.Results After sequencing,two mutations were introduced to hGHR,three eukaryotic expression vectors were identified. The transfected CHO cells expressed wt-hGHR or its mutants. Compared with hGH-wt,two mutate cells (E42K and H56R) had decreased phosphorylated STAT5levels. Conclusion Three CHO cells which stably expresses wide type hGHR and its mutants were successfully established,E42K and H56R partly interfered the phosphorylation of STAT5.

AIM:To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS:Using Diamonsil C_ 18(4.6 mm ?250 mm,5?m)as analytical column.The mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid(B)with gradient elution:0~18 min,83%~80%B;18~30 min,80%~86%B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min;Column temperature was at 35 ℃.RESULTS :A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00~48.00 ?g/mL(r=0.999 3)and 0.64~40.72 ?g/mL(r=0.999 8),respectively.CONCLUSION:The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.

Objective To prepare osmotic pump-controlled release tablets of total flavones in Lysimachia clethroides.Methods Two components of the extract from L.clethroides,rutin and naringenin-7-O-glucoside were used to evaluate the release behavior of osmotic pump controlled release tablets.Single factor investigation was carried out on the membrane compositions and orifice variables,and uniform design was used to optimize the formulation of coating mambrane.Results The membrane weight,PEG400 content,and dibutyl phthalote(DBP) content were the main factors influencing the drug release,and based on 45% and 8.5% of cellulose acetate,respectively,to prepare osmotic pump-controlled release tablets could achieve the desired zero-order release profile.Conclusion The formulation and technology are simple and easy to be carried out.Osmotic pump-controlled release tablets have a stable drug release bahavior and a good reproducibility.

AIM: To prepare the irisquinone freeze-drying injection and investigate its stability. METHODS: The degradation pattern of irisquinone and its effects were measured. At the same time, the stability of freeze-(drying) injection by classic thermostatical test. RESULTS: Irisquinone solution was unstable to heat and its content decreased remarkably in acid and alkaline solutions. The result showed that oil/water partition coeffecient of irisquinone was very large. The irisquinone-hydropropyl1-?-cyclodextrin inclusion complex freeze-drying injection was very effective to enhance the drug stability. CONCLUSION: Irisquinone inclusion complex injection has character of good stability.