Objective To analyze the diffusion properties of acute cerebral stroke to understand the effects of infarct and hematoma on diffusion-weighted MR images of patients with acute cerebral stroke. Methods 25 acute cerebral infarcts, 22 intracranial hematomas and 5 hemorrhagic infarctions were studied with CT, conventional and diffusion MR imaging. The DWI and ADC parameters of acute cerebral stroke were analyzed. Results The DWI images of 25 acute cerebral infarcts, 1 hyperacute hematoma, 2 acute hematomas and 3 subacute hematomas were hyperintensity, and that of 8 early subacute hematomas were hypointensity. The DWI images of early stage of 4 chronic hematomas were hyperintensity at center and hypointensity at periphery, and that of late stage were hypointensity. The DWI images of acute hemorrhagic infarctions had both the hyperintensity and hypointensity. Meanwhile, the ADC images of majority of the lesions were hypointensity. Conclusion DWI could reflect the diffusion properties of different types of cerebral strokes and play an important role in evaluating the strokes.

Objective: To explore whether abnormal brain activity exists in schizophrenics during resting state by fMRI technique and regional homogeneity(ReHo)approach. Methods: fMRI images were obtained in resting state from 18 patients with schizophrenia diagnosed with the DSM-Ⅳ criteria and 18 control subjects. The newly published ReHo approach was applied for fMRI data analysis. Results: Patients with schizophrenia showed decreased ReHo of BOLD signal in resting brain compared with control subjects(P

0.05).FVC increased significantly in week 6 and 12(t=2.762,P =0.008;t=2.255,P =0.029,respectively).There was no statistical significance in FEV_1/FVC between two groups.IC increased significantly in week 6 and 12(t =3.204,P =0.002;t =3.109,P=0.003,respectively).Conclusion Inhaling tiotropium increases IC in patients with stable COPD.As lung function targets judging limit extent of airflow in patients with COPD,FEV_1/FVC and FEV_1(%)are not sensitive in evaluating therapeutic effect of bronchodilators.IC is more reliable than other targets in evaluating therapeutic effect of bronchodilators.

Objective To research the blood supply changes of uterine and fibroid before and after UAE for uterine fibroids.Methods Sixteen patients with symptomatic uterine myoma were treated by Seldinger's bilateral uterine arteries embolization.The same level dynamic enhanced CT scans were undertaken 7 days before and after the procedure with another one 3 months later.The CT values of uterine and fibroid were measured and analyzed. Results 16 cases finished the same level dynamic CT enhancement scans successfully. The dynamic enhanced curves and the CT values showed no significant difference in uterine but had significant difference in fibroids. The fibroids were in a state of ischemia after the procedure.Conclusions Selective uterine arterial embolization is a new safety and effective method for treatment of uterine myoma.

Objective:To investigate the availability for rhIL-18 in the in vitro culture system to stimulate PBMHCs,with inducing cytotoxicities against tumor cells and to analyze the influencing factors for the effects.Methods:The NK cells,T cells and dendritic cells were separated from fresh PBMNCs by using the Stem Sep TM immunomagnetic beads.Cell phenotypes of the purified cell populations were identified with FCM technique.The cell co-culture system was established as follows.The PBMNCs or the cell preparations deleting the definite cell subset with immunoscreening were co-cultivated with mitotic-inactivated tumor cells in the presence of rhIL-18.Cytotoxicities of the various effector cell preparations to a series of tumor cell lines were examined by the isotope releasing assay.Results:In the in vitro cell co-culture system,rhIL-18 rapidly induces activation of the cytolytic responses against various tumor cell lines mediated by PBMNCs.The rapid induction within 24 h of culture was dependent on the dosage of rhIL-18,with the optimal dose of 100 ng/ml of the cytokine.The activated cytotoxicities were abrogated by deleting NK cells prior to the cell co-culture but did not vary when either T cells or DCs were removed.The cytotoxic responses were shown as a pattern of broad-spectrum to the target cells used and were not blocked by anti-MHC moAbs.Conclusion:NK cells were responsible for the rapid induction of cytotoxicities against tumor cells by rhIL-18.

Objective To understand the practice of experiential teaching in the core midwifery curriculum. Methods Phenomenology as a qualitative research method was utilized and fifteen students participated in the non-structured interviews. Results Four themes were found: comprehension of knowledge;professional thought; teacher- student relationship;gratitude. Conclusions Experiential teaching can increase the teaching effect of core midwifery curriculum, and develop good ideological and professional qualities.

Objective To analyze the image diagnosis and differential diagnosis of bone destruction with soft-tissue tumor in sacrum.Methods 26 cases patient proved by surgically and pathologically were retrospectively reviewed, including chordoma (n=8) , giant cell tumor (n=5), tuberculosis(n=5),metastatic cancer(n=3), sarcoma(n=2), and malignant neurofibroma, osteomyelitis, eosinophilic granuloma in one each.Results All cases image manifested as different kinds of bone destruction and soft-tissue tumor in sacrum , pelvis organ were moved.Conclusion A reliable diagnosis can be made by the shape a bone destruction , location characterization of soft-tissue tumor and the age.

BACKGROUND:Currently, surgical treatment for Sanders III type, IV type fractures to restore damaged subtalar surface of the calcaneus and calcaneal shape, in order to reduce the incidence of traumatic arthritis has reached a consensus, and whether bone grafting is selected during surgery for calcaneal fractures has been a controversial issue. OBJECTIVE:To observe the clinical effect of Genex synthetic bone transplants and locking plate on comminuted calcaneal fractures. METHODS:Twenty-one cases of Sanders III type, IV type calcaneal fractures were retrospectively analyzed, including 16 males and 5 females, aged 22 to 55 years. After fracture reduction, a dough-like Genex bone graft was implanted into the defect region via lateral“L”shaped approach, and then the lateral wal of the bone was reset fol owed by internal fixation with the pre-curved locking plate. Fol ow-up observation was performed for fracture healing, Bolher angle and the Maryland Foot Score. RESULTS AND CONCLUSION:Total y 21 patients were fol owed up for 8-16 months. The fractures were healed without displacement, col apse and rejection. The bone graft was degraded within 6 months and completed absorbed after 1 year. According to Maryland Foot Score, the excellent rate was up to 86%, and the Bolher angle was increased from an average preoperative (5.3±3.35)° to postoperative (24.3±1.06)°. Genex artificial bone meal is a biomaterial that can be completely absorbed, has good plasticity and strong supporting force, and it is able to ful y fil bone defects, be easy to fracture reduction, induce bone formation, and promote fracture healing.

Objective To investigate the expression and biological function of microRNA-34a (miR-34a) in bladder cancer.Methods Forty-two cases of bladder cancer specimen,including 17 noninvasive carcinoma and 25 muscle invasive carcinoma classified by UICC 2002 TNM,or 18 low-grade and 24 high-grade classified by WHO 1973.Meanwhile,mucosa adjacent to the carcinoma was selected as normal control.The gene expression of miR-34a was determined using real-time quantitative polymerase chain reaction in 42 cases of bladder carcinoma samples.Mature mimics of miR-34a were chemically synthesized and transiently transfected-intoT24 bladder cancer cells.The effects of miR-34a on apoptosis and proliferation in T24 cells were evaluatedby flow cytometry and MTS respectively.Results 61.9％ of carcinoma samples showed low expression of miR-34a,which was correlated with the malignancy and tumor size of bladder carcinoma.26.5％ (11 cases) was negative and 11.6％ (5 cases) showed high expression.Furthermore,upregulation of miR-34a in T24 cells contributed to cell growth and apoptosis.The apoptosis rate of T24 cells was (9.11 ± 0.41)％,which had significant difference compared with NC mimics and blank control group respectively (P ＜ 0.01).Conclusion The relative low expression of miRNA-34a may be involved in the tumorigenesis and development of bladder carcinoma.

Objective To get the protein expression of sFlt-1 by transfection and to investigate the effects of sFlt-1 gene transfection on the growth of K562 cells.Methods The recombinant plasmid pcDNA3-sFlt-1D4 was constructed.The recombinant plasmid pcDNA3-sFlt-1D4 was transfected into the bone marrow stromal cells by Lipofectamine 2000,which was identified by RT-PCR,ELISA and MTT.Results The transfection efficiency identified by flow cytometry was 9.27%.The protein expression of sFlt-1D4 was found in the culture supernatant 24 h,48 h and 4 weeks after transfetion by ELISA and the expression concentrations were(0.104?0.078),(0.158?0.022) and(0.171?0.069) ?g?L-1,respectively.The content of VEGF secreted by K562 culturing with transfectant cells culture supernatant was reduced compared with control.The inhibitoy rates on the proliferation of K562 cells via MTT assay were 9.41%?4.71%,23.63%?7.50%,and 33.13%?6.93%,respectively.Conclusion The bone marrow stromal cells transfected with recombinant plasmid pcDNA3-sFlt-1D4 could secrete sFlt-1D4 and inhibit the proliferation of K562 cells.