Objective To analyze the diffusion properties of acute cerebral stroke to understand the effects of infarct and hematoma on diffusion-weighted MR images of patients with acute cerebral stroke. Methods 25 acute cerebral infarcts, 22 intracranial hematomas and 5 hemorrhagic infarctions were studied with CT, conventional and diffusion MR imaging. The DWI and ADC parameters of acute cerebral stroke were analyzed. Results The DWI images of 25 acute cerebral infarcts, 1 hyperacute hematoma, 2 acute hematomas and 3 subacute hematomas were hyperintensity, and that of 8 early subacute hematomas were hypointensity. The DWI images of early stage of 4 chronic hematomas were hyperintensity at center and hypointensity at periphery, and that of late stage were hypointensity. The DWI images of acute hemorrhagic infarctions had both the hyperintensity and hypointensity. Meanwhile, the ADC images of majority of the lesions were hypointensity. Conclusion DWI could reflect the diffusion properties of different types of cerebral strokes and play an important role in evaluating the strokes.

0.05).FVC increased significantly in week 6 and 12(t=2.762,P =0.008;t=2.255,P =0.029,respectively).There was no statistical significance in FEV_1/FVC between two groups.IC increased significantly in week 6 and 12(t =3.204,P =0.002;t =3.109,P=0.003,respectively).Conclusion Inhaling tiotropium increases IC in patients with stable COPD.As lung function targets judging limit extent of airflow in patients with COPD,FEV_1/FVC and FEV_1(%)are not sensitive in evaluating therapeutic effect of bronchodilators.IC is more reliable than other targets in evaluating therapeutic effect of bronchodilators.

Objective:To investigate the availability for rhIL-18 in the in vitro culture system to stimulate PBMHCs,with inducing cytotoxicities against tumor cells and to analyze the influencing factors for the effects.Methods:The NK cells,T cells and dendritic cells were separated from fresh PBMNCs by using the Stem Sep TM immunomagnetic beads.Cell phenotypes of the purified cell populations were identified with FCM technique.The cell co-culture system was established as follows.The PBMNCs or the cell preparations deleting the definite cell subset with immunoscreening were co-cultivated with mitotic-inactivated tumor cells in the presence of rhIL-18.Cytotoxicities of the various effector cell preparations to a series of tumor cell lines were examined by the isotope releasing assay.Results:In the in vitro cell co-culture system,rhIL-18 rapidly induces activation of the cytolytic responses against various tumor cell lines mediated by PBMNCs.The rapid induction within 24 h of culture was dependent on the dosage of rhIL-18,with the optimal dose of 100 ng/ml of the cytokine.The activated cytotoxicities were abrogated by deleting NK cells prior to the cell co-culture but did not vary when either T cells or DCs were removed.The cytotoxic responses were shown as a pattern of broad-spectrum to the target cells used and were not blocked by anti-MHC moAbs.Conclusion:NK cells were responsible for the rapid induction of cytotoxicities against tumor cells by rhIL-18.

Objective To research the blood supply changes of uterine and fibroid before and after UAE for uterine fibroids.Methods Sixteen patients with symptomatic uterine myoma were treated by Seldinger's bilateral uterine arteries embolization.The same level dynamic enhanced CT scans were undertaken 7 days before and after the procedure with another one 3 months later.The CT values of uterine and fibroid were measured and analyzed. Results 16 cases finished the same level dynamic CT enhancement scans successfully. The dynamic enhanced curves and the CT values showed no significant difference in uterine but had significant difference in fibroids. The fibroids were in a state of ischemia after the procedure.Conclusions Selective uterine arterial embolization is a new safety and effective method for treatment of uterine myoma.

Objective To understand the practice of experiential teaching in the core midwifery curriculum. Methods Phenomenology as a qualitative research method was utilized and fifteen students participated in the non-structured interviews. Results Four themes were found: comprehension of knowledge;professional thought; teacher- student relationship;gratitude. Conclusions Experiential teaching can increase the teaching effect of core midwifery curriculum, and develop good ideological and professional qualities.

Objective: To explore whether abnormal brain activity exists in schizophrenics during resting state by fMRI technique and regional homogeneity(ReHo)approach. Methods: fMRI images were obtained in resting state from 18 patients with schizophrenia diagnosed with the DSM-Ⅳ criteria and 18 control subjects. The newly published ReHo approach was applied for fMRI data analysis. Results: Patients with schizophrenia showed decreased ReHo of BOLD signal in resting brain compared with control subjects(P

Objective To analyze the image diagnosis and differential diagnosis of bone destruction with soft-tissue tumor in sacrum.Methods 26 cases patient proved by surgically and pathologically were retrospectively reviewed, including chordoma (n=8) , giant cell tumor (n=5), tuberculosis(n=5),metastatic cancer(n=3), sarcoma(n=2), and malignant neurofibroma, osteomyelitis, eosinophilic granuloma in one each.Results All cases image manifested as different kinds of bone destruction and soft-tissue tumor in sacrum , pelvis organ were moved.Conclusion A reliable diagnosis can be made by the shape a bone destruction , location characterization of soft-tissue tumor and the age.

Objective To investigate the effects of Acinetobacter baumannii culture supernatants on planktonic cell growth and biofilm formation of Pseudomonas aeruginosa.Methods The standard isolates (ATCC 19606,ATCC 1195) and clinical isolates (AB23,AB39,AB53) of Acinetobacter baumannii were collected and the 6,12,16,24 and 48 hour-cultured supernatants were extracted.The effects of the culture supernatants on the biofilm formation of Pseudomonas aeruginosa PAO1 were detected on the 96-well plate combined with crystal violet staining.Two-fold concentration of LB medium was prepared to eliminate the effects of nutrition consumption of Acinetobacter baumannii during culture on Pseudomonas aeruginosa growth.The active ingredients in the supernatant of Acinetobacter baumannii culture medium were investigated by using the concentrated tube containing protein with relative molecular mass 3 000.Results The most suitable period for Acinetobacter baumannii culture supernatant extraction was between 12 to 24 hours,so the 16 hourcultured supernatant was chosen for next experiments.The 50％ culture supernatant of Acinetobacter baumannii ATCC 1195 and ATCC 19606 significantly inhibited the planktonic cell growth of Pseudomonas aeruginosa PAO1,in which the absorbance at 630 nm reduced from(0.688 ± 0.014) and(0.692 ± 0.014) to (0.431 ± 0.023) and (0.428 ± 0.020) respectively (t =16.780,P ＜ 0.05;t =18.500,P ＜ 0.05).The 50％ culture supernatant of Acinetobacter baumannii ATCC 1195 and ATCC 19606 also significantly inhibited the biofilm formation of Pseudomonas aeruginosa PAO1 with decreased absorbance at 570 nm from (2.071 ± 0.068) and (1.986 ±0.023) to (1.639 ± 0.042) and (1.525 ± 0.202) respectively (t =9.358,P ＜ 0.05;t =3.924,P ＜ 0.05).The biofilm inhibitory effect of the protein with relative molecular mass less than 3 000 was obviously observed by reducing amount of biofilm formation from (1.177 ± 0.040) to(1.056 ± 0.030) (t =4.192,P ＜ 0.05),while there was no inhibitory effect of the proteins with relative molecular mass more than 3 000 in the composition.Conclusion Acinetobacter baumannii culture supernatant could effectively inhibit the planktonic cell growth and biofilm formation of Pseudomonas aeruginosa and the relative molecular mass of active ingredients in the culture supernatant may be less than 3 000.

Objective To know the influence of wanning traditional Chinese medicine dipping for newborns on their sleeping quality and appetite. Methods Divided 600 newborns into the traditional Chinese medicine dipping group, swimming group and the single bath group according to their sequence of birth, there were 200 cases in each group. Different nursing cares were used in the different group sepa-rately. Compared the condition of sleep quality and appetite among the three groups. Results The condi-tion of sleep quality and appetite in the traditional Chinese medicine dipping group was significant better than those in the other groups with few cry and scream. Conclusions Wanning traditional Chinese medicine dipping for newborns can ameliorate their quality of sleep and improve their aapetite.

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.