1.Effect of interventional treatment on absolute alcohol in different types of liver cancer
Xianlan LIANG ; Liying QI ; Quanqing MI ; Lihua FENG
Clinical Medicine of China 2015;31(1):39-42
Objective To investigate the effect of routine ultrasound examination and interventional treatment of absolute alcohol in different types of liver cancer.Methods Thirty cases of liver cancer patients who were treated in the 3rd People' s Hospital of Yangquan from May 2010 to May 2014 were selected as ours subjects.They were performed ultrasound exam.The ultrasound characteristic was recorded.Patients were divided into intrahepatic cholangiocarcinoma (ICC) group (11 cases),hepatocellular cholangiocarcinom (HCC) group (14cases) and mixed cholangiocarcinoma group(5 cases) based on pathology results.Nine HCC postoperative recurrence patients and 7 ICC postoperative recurrence patients were treated with absolute alcohol and the clinical effect was observed.Results There were significant differences in the three groups in terms of gender,boundary definition and tumor location (x2 =8.01,6.16,7.32 ; P < 0.05).While the distribution of ultrasound echo intensity was not statistically significant(x =3.44,P > 0.05).The effective rate of interventional treatment of absolute alcohol in HCC patients was 88.89%,and 42.86% in ICC group,and the difference was statistically significant (x2 =3.88,P < 0.05).Conclusion The ultrasound characteristics of different pathological liver cancer are different.Ethanol interventional therapy on HCC patients is significantly better than the effect of ICC patients,suggesting that ICC postoperative recurrence patients can be treated combined with other methods to help improve the effect.
2.Clone of Full-length cDNA Sequence of Actin Gene of Eleutherococcus Senticosus and Bioinformatics Analysis
Lihua CHAI ; Leshan XIU ; Mi ZHOU ; Zhaobin XING
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(9):1901-1906
This study was aimed to clone the full-length cDNA sequence of actin gene of Eleutherococcus sentico-sus. And bioinformatics analysis was used. The total RNA was isolated from leaves of E. senticosus , and cDNA was synthesized by reverse transcription of total RNA. Primers were designed according to the conserved se-quence that had been cloned of Actin of E. senticosus . Then, the 3'and 5' cDNA fragments were cloned by nested PCR . The full-length gene was obtained by gene splicing method . Sequencing results were compared and treated with similarity analysis by blast analysis in the GenBank. Protein secondary structure and tertiary struc-ture of Actin of E. senticosus was predicted by online software. The results showed that the full-length cDNA of Actin of E. senticosus is 1507 bp, which named EsActin1, GenBank accession No. KC469585. The conserved sequence, which contained a 1134 bp open reading frame that encoding a 377 amino acid residues, a 5'-UTR of 140 bp and a 3'-UTR of 233 bp. Homologous alignment showed that it shared over 75% nucleotide se-quence similarity and over 94% amino acid sequences similarity with Actins in other plants. It was concluded that this study first isolated and reported the full-length cDNA sequence of actin gene of E. senticosus , and laid a foundation for the molecular biology research of E. senticosus .
3.Relationship between Fractional Anisotropy of Corticospinal Tract and Motor Function in Stroke Patients Using Diffusion Tensor Imaging
Lihua ZHANG ; Lixin MI ; Longjun GUO ; Luyi WANG
Chinese Journal of Rehabilitation Theory and Practice 2017;23(7):802-806
Objective To explore the relationship between fractional anisotropy (FA) of corticospinal tract (CST) and motor function in stroke patients using diffusion tensor imaging (DTI). Methods From January, 2013 to October, 2016, 36 stroke patients were evaluated with Fugl-Meyer Assessment (FMA), Japan Upper Limb Function Test and DTI before and eight weeks after rehabilitation. FA in posterior limb of internal capsule, cerebral peduncle and pons were obtained, including ipsilesional and contralesional CST. Results The FA values within three sections of CST were significantly lower in the ipsilesional side than in the contralesional side both before and after rehabilitation (t>5.330, P<0.001). In posterior limb of internal capsule, the FA value increased after rehabilitation (t=-3.415, P<0.05), and no significant dif-ference was found in the other two sections (t<1.653, P>0.05) in the ipsilesional side, as well as in all the sections in the contralesional side (t<1.021, P>0.05). The FA values were positively correlated with the scores of FMA and Japan Upper Limb Function Test in all the sections both before and after rehabilitation (r>0.43, P<0.05), especially the relationship between the FA value and the score of Japan Upper Limb Function Test in the posterior limb of internal capsule (r=0.67). Conclusion DTI can be used to evaluate CST injury and the relationship be-tween CST injury and motor function.
4.Relationship between Corticospinal Cord Tract and Upper Limb Function in Stroke Patient: A Diffusion Tensor Imaging Study
Lihua ZHANG ; Longjun GUO ; Shuyan QIE ; Lixin MI ; Luyi WANG ; Qiaoxia ZHEN
Chinese Journal of Rehabilitation Theory and Practice 2016;22(1):103-105
Objective To observe the relationship between injury of corticospinal cord tract (CST) in basal ganglia and upper limb func-tion after stroke using diffusion tensor imaging (DTI). Methods 18 stroke inpatients hospitalized from January, 2013 to July, 2015 accepted DTI, and their upper limb function was evaluated with simple Fugl-Meyer Assessment and Japan Upper Limb Function Test. The fractional anisotropy (FA) of CST in basal ganglia of affected and unaffected sides were compared, and the correlation between FA of affected CST and upper limb motor function were analyzed. Results The FA was significantly less in the affected CST than in the unaffected CST (t=-21.09, P<0.001). The FA of the affected CST correlated with the scores of simple Fugl-Meyer Assessment (r=0.570, P<0.05) and Japan Upper Limb Function Test (r=0.509, P<0.05). Conclusion CST is injured after stroke, which may related to the upper limbs motor function impairment.
5.Clinical analysis of cervical screening in 2329 pregnant women.
Ying WANG ; Yanhong YU ; Lin XIAO ; Xianjun MI ; Lihua FU ; Yanyan LUO
Journal of Southern Medical University 2014;34(9):1355-1358
OBJECTIVETo study the cytopathologic characteristics of cervical diseases in pregnant women and the outcomes of the postpartum women to provide evidence for prevention and treatment of cervical cancer.
METHODSThis study was conducted among 2329 pregnant women undergoing routine gestational examinations between September, 2012 and September, 2013. The women with abnormal cytological findings by Thin-prep cytology test (TCT) were followed up and colposcopy and cervical biopsy were performed. The TCT results of these women were compared with those of 32 491 non-pregnant women in Zhongshan Cervical Cancer Mass Screening Program.
RESULTSOf the 2329 pregnant women, a total of 97 patients had abnormal TCT results (4.16%). Cervical biopsy were performed for 14 patients (14.43%), and 8 (57.14%) of them had evidence of cervical intraepithelial neoplasia (CIN) or cancer on biopsy. In the 32491 non-pregnant women in the mass screening program, 1383 (4.26%) women had abnormal TCT results and cervical biopsy were performed for 248 patients (17.93%), among whom 148 (59.68%) had evidence of CIN or cancer on biopsy. The rate of high-grade squamous intraepithelial lesion (HSIL) was significantly higher in non-pregnant women than in pregnant women (P=0.033), but the total rate of cytological abnormalities were comparable between them (P=0.911). The patients with CIN had regular examinations during pregnancy and postpartum follow-up showed no invasive carcinoma.
CONCLUSIONPregnancy is not a risk factor to accelerate the progress of cervical lesions, and most of the cervical lesions are relieved or show no progression in the postpartum women, suggesting the feasibility of follow-up during pregnancy and postpartum reevaluation for patients with CIN in pregnancy.
Biopsy ; Cervical Intraepithelial Neoplasia ; diagnosis ; Colposcopy ; Cytodiagnosis ; Female ; Humans ; Mass Screening ; Postpartum Period ; Pregnancy ; Pregnancy Complications, Neoplastic ; diagnosis ; Uterine Cervical Neoplasms ; diagnosis
6.Study on operation service quality and safety management standard
Yuehui LIU ; Wenyi ZHANG ; Weidong MI ; Li HE ; Yongbin LI ; Jia LI ; Lihua LIU
Chinese Journal of Hospital Administration 2018;34(12):979-983
This study aimed at the quality and safety improvements of surgical services in terms of preoperative, intraoperative and postoperative sections, and at establishing corresponding restraint standards. To this end, such methods as literature analysis, dispute case analysis, surgical adverse event analysis, and system standard interpretation as well as clinical experts survey were called into play, to identify key elements in terms of the universality, high-prevalence rate, criticality and impact degree. A standard framework is established focusing on surgical service and such nodes as key components of the event; a standard text on surgical service quality and safety management is developed, comprising three sections, 8 nodes, 27 key elements and 82 standards.
7.Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway
Qihong WANG ; Na ZHANG ; Lihua HU ; Yong XI ; Wenxin MI ; Yindong MA
Tissue Engineering and Regenerative Medicine 2020;17(2):183-192
Background:
This study aims to investigate the effect of integrin β1 on wound healing induced by adipose-derived stem cells (ADSCs), as well as the corresponding mechanism.
Methods:
Integrin β1 was overexpressed in ADSCs. Thereafter, flow cytometry and transwell chambers technology were used to measure the endothelial-like differentiation (CD31 as a biomarker of endothelial cell) and cell migration, respectively. Western blot was used to detect the activation of PI3K/AKT, NF-κB and ERK signaling pathways. The effects of integrin β1 overexpression on healing time, healing rate and fibroblast number were further evaluated in the rat models of chronic refractory wound.
Results:
The overexpression of integrin β1 increased CD31+ endothelial-like cells (about 3.6-fold), promoted cell migration (about 1.9-fold) and enhanced the activation of PI3K (p-PI3K; about 2.1-fold) and AKT (p-AKT; about 2.2-fold). These effects were all weakened when PI3K/AKT pathway was inhibited by LY294002 treatment. In addition, the experiments in rat wound models showed that integrin β1 overexpression obviously shortened healing time (approximately 0.41-fold), increased healing rate (about 2.7-fold, 2.8-fold and 1.6-fold at day 7, 14 and 21) and increased the number of fibroblasts (approximately 3.1-fold at day 21). All of the above differences were statistically significant (p < 0.05).
Conclusion
Integrin β1 can promote the migration and endothelial-like differentiation of ADSCs by activating PI3K/AKT pathway and then enhance the function of ADSCs in promoting wound healing.
8. Synchronous lymphoma and carcinoma-clinical analyses of 17 patients
Yuanyuan XIONG ; Zhijuan LIU ; Lin CHEN ; Fangfang YUAN ; Qingsong YIN ; Ruihua MI ; Bing ZHANG ; Jianwei DU ; Qinglan ZHANG ; Quande LIN ; Li’na ZHANG ; Xue GAO ; Lihua DONG ; Yufu LI ; Yongping SONG ; Xudong WEI
Chinese Journal of Hematology 2018;39(4):277-280
Objective:
To explore the clinical features of patients with synchronous lymphoma and carcinoma.
Methods:
The clinical data of 17 patients with Synchronous lymphoma and carcinoma from February 2012 to October 2017 were analyzed retrospectively.
Results:
Among 17 patients of lymphoma, 1 case HL, 2 cases B-NHL, 6 cases MZBL, 3 cases DLBCL, 1 case mantle cell lymphoma (MCL) , 3 cases NK/T- cell lymphoma, 1 case anaplastic large cell lymphoma(ALCL). In terms of 17 patients with carcinoma, 3 cases esophageal carcinoma, 3 cases gastric carcinoma, 2 cases colorectal carcinoma, 7 cases thyroid carcinoma, 1 case hepatocellular carcinoma and lung cancer. Up to 15 patients received operation, and some of them combined with chemotherapy, radiotherapy and autologous transplant. Follow-up analysis showed that 3 cases was undergoing treatment, 2 cases lost follow-up, 4 cases died, 3 cases achieved CR, 3 cases remained to be at SD, and 2 cases assessed for progression or recurrence.
Conclusion
The relationship between lymphoma and carcinoma was under discussion, patients with synchronous lymphoma and carcinoma were not unusual. We herein should raise awareness to avoid misdiagnosis.
9.Effect of early scream sound stress on learning and memory in female rats.
Lili HU ; Bo HAN ; Xiaoge ZHAO ; Lihua MI ; Qiang SONG ; Chen HUANG
Journal of Central South University(Medical Sciences) 2015;40(12):1371-1376
OBJECTIVE:
To investigate the effect of early scream sound stress on the ability of spatial learning and memory, the levels of norepinephrine (NE) and corticosterone (CORT) in serum, and the morphology of adrenal gland.
METHODS:
Female Sprague-Dawley (SD) rats were treated daily with scream sound from postnatal day 1(P1) for 21 d. Morris water maze was used to measure the spatial learning and memory ability. The levels of serum NE and CORT were determined by radioimmunoassay. Adrenal gland of SD rats was collected and fixed in formalin, and then embedded with paraffin. The morphology of adrenal gland was observed by HE staining.
RESULTS:
Exposure to early scream sound decreased latency of escape and increased times to cross the platform in Morris water maze test (P<0.05). The levels of serum NE was significantly elevated, with an obvious tendency in the increase of CORT level (P<0.05). The morphology of adrenal gland was also influenced.
CONCLUSION
Newborn female rats subjected to scream sound stress can enhance spatial learning and memory ability in adulthood, which is related to activation of the hypothalamo-pituitary-adrenal axis and sympathetic nervous system.
Adrenal Glands
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anatomy & histology
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Animals
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Corticosterone
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blood
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Female
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Maze Learning
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Memory
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Norepinephrine
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blood
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Psychoacoustics
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Rats
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Rats, Sprague-Dawley
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Stress, Psychological
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Vocalization, Animal
10.Interference of CD38 monoclonal antibody in blood compatibility testing and its countermeasures: A general consensus among experts
Jianqing MI ; Xiaohong CAI ; Shaoyuan WANG ; Lihua HU ; Ting NIU ; Deqing WANG ; Chengcheng FU ; Chunyan SUN ; Dong XIANG ; Wen GAO ; Tianhong MIAO ; Liye ZHONG ; Baohua QIAN ; Gang AN ; Rong XIA ; Rong GUI ; Jing LIU ; Xiaofeng TANG ; Jue XIE ; Jia GAN ; Jiang WU ; Danhui FU ; Li QIN ; Jian HOU ; Xuefeng WANG
Chinese Journal of Blood Transfusion 2021;34(4):327-334
With continuous discovery of tumor immune targets and continuous changes in antibody research and development technology, antibody drugs are becoming more and more widely used in clinical practice. However, some targets are not only expressed on tumor cells, but also on red blood cells. Therefore, the clinical application of antibodies against the corresponding targets may interfere with the detection of blood transfusion compatibility, resulting in difficulty in blood matching or delay of blood transfusion. This consensus summarizes the current solutions for the interference of CD38 monoclonal antibody (CD38 mAb) in transfusion compatibility testing. After analyzing the advantages and disadvantages of different methods, polybrene and sulfhydryl reducing agents [dithiothreitol (DTT) or 2-mercaptoethanol (2-Me)], as a solution for CD38 mAb interference in blood compatibility testing, are recommended for Chinese patients, so as to eliminate blood transfusion interference produce by CD38 mAb and further provide a pre-transfusion workflow for clinicians and technicians in Department of Blood Transfusion.