MicroRNAs (miRNAs or miRs) are a class of small non-protein-coding RNAs, approximately 18 to 25 nt long. MicroRNAs can act as endogenous RNA interference. MicroRNAs can posttranscriptionally regulate the expression of hundreds of their target genes, controlling a wide range of biological functions such as cellular proliferation, differentiation, and apoptosis. MicroRNAs can posttranscdptionally regulate the expression of genes by hybridizing to complementary sequences in 3' UTR (3' untranslated region) of target messenger RNA (mRNA), repressing the translation of mRNA or increasing the instability of mRNA. A number of miRNAs are mapped to cancer-associated fragile regions (FRAs) as well as in minimal regions of loss of heterozygosity, minimal regions of amplification, or common breakpoint regions in the genome, suggesting that miRNAs might be involved in tumorigenesis. Many miRNAs are up or down-regulated in cancers as potential oncogene or tumor suppressors. It has been considered that the mutation of a series of oncogene or anti-oncogene gradually causes tumorigenesis. The conventional points of view were changed with the finding of non-protein-coding RNAs. MiRNA as members of non-protein-coding RNAs may play an important role in regulating tumor formation. Recent studies have in-vestigated the relationship of miRNAs with neoplasia, development, treatment and prognosis. Lung cancer is the leading cause of cancer-related deaths all over the world. Its etiology is primarily genetic and epigenetic damage caused by tobacco smoke. Systematic analysis of RNA and protein expression levels of thousands of genes has also contributed to defining the molecular net work of lung carcinogenesis. MiRNAs are closely related to lung cancer and play an important part in the diagnosis, therapy, surveillance and prognosis of lung cancer. We reviewed some miRNAs closely associated with lung cancer such as miRNA-126, miRNA-221, miRNA-222, has-mir-221, a polycistronic microRNA cluster miR-17-92, miRNA-128b, has-mir-137, has-mir-182, has-mir-372 and miRNA let-7. We summarized the roles of miRNAs in the genetic susceptibility, invasion or metastasis, diagnosis, therapy and prognosis of lung cancer.

Objective Exploring the relationship among plasma tumor necrosis factor alpha(TNF-?),endothelin-1(ET-1),interleukin-6(IL-6) and cardiovascular diseases in patients with sleep apnea.Methods ELISA method were used to measure circulating levels of TNF-?,ET-1 and IL-6 in 15 with SAS complicated with cardiovascular diseases and 15 normal subjects, matched in the age ,sex ,height and weight.Results Plasma TNF-?,ET-1 and IL-6 level had increased significantly in SAS group,as compared with normal subjects(P

Objective To understand tigecycline and commonly used anti-bacterial agents resistance in Gram-positive cocci and Acinetobacter baumannii .Methods 445 strains of Gram-positive cocci and 83 strains of Acinetobacter baumannii were collected . VITEK 2 Compact automated bacterial identification and susceptibility analysis system was employed to identify Gram-positive coc-ci and Acinetobacter baumannii and their bacterial sensitivities toward tigecycline and commonly used anti-bacterial agents were de-tected by broth microdilution method for antimicrobial susceptibility testing (MIC assay) and Kirby-Bauer disk diffusion susceptibil-ity test(K-B) ,respectively .Results 155(34 .8% ) strains of Staphylococcus aureus ,78(17 .5% ) Enterococcus feces ,56(12 .6% ) Staphylococcus haemolyticus ,45(10 .1% ) Staphylococcus epidermidis ,43(9 .7% ) Enterococcus faecalis ,18(4 .1% ) human Staphy-lococcus ,16(3 .6% ) Wolfowitz Staphylococcus ,8(1 .8% ) birds Enterococci and 26(5 .8% )other strains were found in 445 cases of Gram-positive cocci .The rate of penicillin-resistant Staphylococci was 92 .4% and the rates of Staphylococci′s resistance to eryth-romycin ,clindamycin ,oxacillin and ciprofloxacin were all over 50% .Enterococci ,which was sensitive to linezolid and tigecycline ,was resistant naturally to many drugs .The tigecycline sensitive rate ,intermediate rate and resistance rate acquired by MIC assay for multi-drug resistant and pan-resistant Acinetobacter baumannii were 57 .1% (47/83) ,42 .9% (36/83) and 0 .0% (0/83) ,respec-tively ,while those by K-B methods were 2 .4% (2/83) ,40 .5% (34/83) and 57 .1% (47/83) ,respectively .Conclusion Tigecycline showes good antibacterial activity toward Gram -positive cocci and Acinetobacter baumannii .

Objective To explore the relationship between the quality of head nurses and nursing quality. Methods Analyses of data were conducted by means of linear regression analysis and stepwise linear regression analysis. Results In the regression analysis of the overall quality of head nurses and nursing quality, t=2 974,P

It is necessary to determine the position of break points in order to study the structure rearrangement of chromosomes and gene mapping. Therefore it is indispensable to set up region, banded idiogram and nomenclature system. Nesbitt and Franke reported an idiogram and a nomenclature system for band patterns of mouse chromosomes which thereafter was accepted by the Committee on Standardized Genetic Nomenclture. Nevertheless, there are few data of systematical contrast studies in this domain. There are still difficulties for distinguishing between some chromosomes because they are similar and variability. In this paper, we compared our G-banding karyotypes with the banding patterns of Nesbitt's idiogram. Our 400 karyotypes come from bone marrow cells and fibroblasts of several normal inbred and outbred mice. Each banding obtained from each well banded chromosomes of different cells were contrasted to that of Nesbitt's idiogram. The results are as follows: 1. we have not found so many bands in one metaphase chromosome banded with trypsin-Giemsa technique as reported by Nesbitt. 2. The banding numbers and positions on each well banding chromosome picked out from different metaphase plates were consistent with those of Nesbitt's idiogrom. But some bands such as 1E1 and 1E2; 2E5-2F5; 4A3-4A5; 5C1-5C3 can't usually be distinguished in our data. 3. We observed that the major banding structure of each karyotype remains stability, but the minor banding structure appears to be variablitity in inbred mice. In addition, 6 variant pictures and region idiograms in the G-banding structure of each chromosome were showed and some notices for correct analysis of mouse karyotype were discussed.

Objective To describe practice experience of the male nurses in obstetrics and gynecology department.Methods Phenomenological methodology was adopted in this study.In-depth interview was conducted on 12 male nurses who practiced in obstetrics and gynecology department from March 2012 to May 2013.Data were analyzed by Colaizzi's analysis method.Results The main experience included:lack of patient understanding and support,a sense of loss,decline in the enthusiasm to learn,inconvenience of live and study,dissatisfied with specialist operations.Conclusions Male nurses in obstetrics and gynecology has many adverse experiences,clinical teachers should take measures to help them successfully complete the practice and prevent occurrence of adverse experience.

Objective To investigate the clinical application value of three-dimensional ultrasonic inspection in prenatal diagnosis.Methods 90 cases of fetal formations were Tetrospecthely analysed,which were diagnosed by two-dimensionul associtated with three-dimensional ultrasonic inspection.We compared the ultrasonic inspection with the clinical diagnosis.Results The all coincidence rate was 100%by combining the two measures,three-dimensional ultrasonic inspection can make up the defect of two-dimemional ultrasonic inspection.Conclusion Two-dimensional combined with three-dimensional ultrasonic inspection can accurately diagnose fetal formations,and increase the detection rate.

Objective This study was designed to examine the expression of VEGF and its correlation with clinicopathological factors of cervical carcinoma. Methods mmunohistochemistry S-P method was used to detect the expression of VEGF in 10 cases of nomal cevical epithelium( NCE), 18 cases of cervical intraepithelial neoplasm (CIN) and 77 cases of early invasive carcinoma of cervix (ICC). Results The positive rate of VEGF protein in NCE,CIN,ICC was 10% (1/10) ,44.4% (8/18) ,74. 0% (57/77), respectively. The defference was significant (P ＜0. 05). Expresson of VEGF was not related with tumor stage, histologic types and stroma infiltration, but related with cell differentiation and intravascular involvement、lymph node metastasis. Conclusion VEGF protein had a positive correlation with cell differentiation and intravascular involvement 、 lymph node metastasis. the expression of VEGF gene result in the occurrence and development of cervical carcinoma by inducing the angiogenesis.

AIM: To investigate the anti-cancer effect of cytotoxic T lymphocytes (CTL) activated by sensitized dendritic cells (DCs). METHODS: Immature DCs were induced in vitro from peripheral blood monocytic cells (PBMC) and sensitized by adding tumor cells antigen extract. DCs were identified by their morphology and surface markers. MTT assay was used to evaluate the killing activity of CTL activated by sensitized DCs. The effects of specific CTL cells on inhibiting transplanted tumor HT-29 growth and on preventing HT-29 tumor generation were evaluated by injecting CTL into nude mice. RESULTS: After cultured for seven days, a large number of activated DCs were obtained with typical morphology, extensive stimulatory proliferation capacity and high CD80 (63.5%), CD83 (67.6%) and CD3/HLA-DR (83.2%) expressions. The killing activity of CTL at 20∶1 ratio of effective cells to target cells was more than 75% to tumor cells, 35%-45% to homologous cell line and weaker to other germ cell line (P

Objective:To examine the serum proteomic spectra of human esophagial carcinoma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS),so as to set up a diagnostic model of esophagial carcinoma and to investigate its clinical value. Methods:Thirty-two esophagial carcinoma patients and 28 healthy controls were obtained from Fourth Affiliated Hospital of Hebei Medical University during May to September of 2008. Serum protein was extracted by weak cation exchange (WCX) protein chip system,and proteomic spectra was examined by MALDI-TOF MS. The obtained data were analyzed by ZUCI-protein chip data analyze system (ZUCI-PCDAS) and an esophagial carcinoma diagnostic model was established by genetic arithmetic (GA) combined support vector machine (SVM). The above 60 samples were randomly divided into training set and blinding test set,with training set including 21 esophagial carcinoma patients and 19 healthy controls and blinding test set including 11 esophagial carcinoma patients and 9 healthy controls,so as to examine the specificity and sensitivity of this diagnostic model. Results:Serum proteomic spectra of esophagial carcinoma patients and healthy controls were obtained by MALDI-TOF MS,and m/z (mass to charge) peaks of 44 differential proteins were obtained after analyzed by ZUCI-PCDAS software package (P