OBJECTIVE: To observe the expression of cyclooxygenase-2(COX-2) in atherosclerotic tissue in rabbits and to discuss the association between COX-2 and atherosclerosis (AS) and evaluate the effect of cyclooxygenase-2(COX-2) inhibitor celecoxib (cele) on atherosclerosis progression in atherosclerotic model rabbits. METHODS: Eighteen New Zealand rabbits were randomly divided into three groups: normal control group, high cholesterol (HC) group, and celecoxib(20 mg?kg?d-1) group. After feeding for 14 weeks, the aortic atherosclerotic lesions and the ratio of aortic intima to media in rabbits were measured. RT-PCR, immunohistochemistry and Western Blot were used for the detection of expression of COX-2 mRNA and protein. RESULTS: In celecoxib-treated group compared with HC group, the atherosclerotic lesion area, the ratio of aortic intima to media, the expression of COX-2 mRNA and protein were all significantly reduced (P

Objective To study the effect of angiotensin Ⅱ type 1 receptor antagonist Irbesartan on the expressions of cyclooxygenase-2(COX-2)in rabbit model of atherosclerosis.Methods Thirty male New Zealand rabbits were randomly divided into five groups:normal control group,high cholesterol group(Group HC),10 mg?kg-1?d-1 Irbesartan group(Group S),20 mg?kg-1?d-1 Irbesartan group(Group M),30 mg?kg-1?d-1 Irbesartan group(Group H).Except the control group,the other four groups were fed with high cholesterol diet to induce atherosclerosis for 14 weeks.Then,the size of aortic atherosclerotic lesions and the ratio of aortic tunica intima to media were measured.RT-PCR,immunohistochemistry and Western blotting were used for COX-2 mRNA and protein levels respectively.Results Compared with Group HC,atherosclerotic lesion size was markedly reduced in Groups M and H(P

Objective To investigate the effect of sevoflurane on adriamycin-induced damage to primary neonatal rat cardiomyocytes.Methods Primary neonatal rat cardiomyocytes were isolated from Sprague-Dawley rats (within 24 h after birth) and cultured in DMEM liquid culture medium.The cells were seeded in 96-well plates (200μl/hole) or 6-well plates (2 ml/hole) with a density of 5 × 104/ml and randomly divided into 4 groups (n =24 each):control group (group C),adriamycin group (group A),sevoflurane group (group S) and adriamycin + sevoflurane group (group AS).In groups A and AS,adriamycin 1 μmol/L was added to the cultured medium,the equal volume of PBS was added instead in groups C and S,and the cells were then incubated for 24 h.The cells were exposed to 2.4％ sevoflurane for 2 h starting from 24 h of incubation in groups A and AS.The cell viability,concentrations of cTnI and N-terminal pro-B-type natriuretic peptide (NT-proBNP) in the culture medium,and expression of Bax and Bcl-2 were detected 2 h after the end of exposure to sevoflurane.The Bax/Bcl-2 ratio was calculated.Results Compared with group C,the cell viability and expression of Bcl-2 were significantly decreased,while the concentrations of cTnI and NT-proBNP in the culture medium,expression of Bax and Bax/Bcl-2 ratio were significantly increased in group A,and the cell viability was decreased in group AS (P ＜ 0.05).The expression of Bcl-2 was significantly higher and the concentrations of cTnI and NT-proBNP in the culture medium,expression of Bax and Bax/Bcl-2 ratio were significantly lower in group AS than in group A (P ＜ 0.05).Conclusion Sevoflurane can reduce adriamycin-induced damage to primary neonatal rat cardiomyocytes and inhibition of cell apoptosis is involved in the mechanism.

Objective To evaluate the role of phosphatidylinositol 3-kinase/protein-serine-threonine kinases (PI3K/Akt) signaling pathway and autophagy in reduction of adriamycin-induced myocardial injury by sevoflurane in the rats.Methods Thirty-six healthy male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 6 groups (n =6 each) using a random number table:control group (group C),adriamycin-induced myocardial injury group (group Dox),sevoflurane group (group Sev),LY294002 inhibitor group (group LY),solvent control group (group dimethyl sulfoxide [DMSO]),and 3-MA inhibitor group (group 3-MA).Adriamycin 4 mg/kg was injected intraperitoneally once a week for 3 weeks in all the groups except group C.The rats were mechanically ventilated for 2 h in C and Dox groups.The rats inhaled 2.4％ sevoflurane for 2 h in group Sev.In group LY,0.3 mg/kg LY294002 was injected via the tail vein at 10 min before anesthesia,and the rats inhaled 2.4％ sevoflurane for 2 h.In group DMSO,the equal volume of DMSO was injected,and the rats inhaled 2.4％ sevoflurane for 2 h.After the blood samples were collected from the heart,the rats were sacrificed,and myocardial specimens were obtained for determination of cardiac troponin Ⅰ (cTnI) concentrations in serum (by enzyme-linked immunosorbent assay),expression of total Akt (t-Akt),phosphorylated Akt (p-Akt),mammalian target of rapamycin (mTOR),phosphorylated mTOR (p-mTOR) and autophagy marker microtubule-associated protein light chain 3 Ⅱ (LC3 Ⅱ) (by Western blot),and cell apoptosis (by TUNEL).Apoptosis index (AI) was calculated.Results Compared with group C,the expression of p-Akt and p-mTOR was significantly down-regulated,and the expression of LC3 Ⅱ,AI,and serum cTnI concentration were significantly increased in the other five groups (P＜ 0.05).Compared with group Dox,the expression of p-Akt and p-mTOR was significantly up-regulated,and the expression of LC3 Ⅱ,AI,and serum cTnI concentration were significantly decreased in group Sev (P＜0.05).Compared with group Sev,the expression of p-Akt and p-mTOR was significantly down-regulated,and the expression of LC3 Ⅱ,AI,and serum cTnI concentration were significantly increased in group LY,and the expression of LC3 Ⅱ was significantly down-regulated,and serum cTnI concentrations were significantly decreased in group 3-MA (P＜0.05).Conclusion Sevoflurane can activate PI3K/Akt signaling pathway and inhibit autophagy,thus reducing adriamycin-induced myocardial injury in rats.

Objective To identify major factors which affect the completion quality of projects and build predictive models,and to propose strategies and suggestions for total process quality management of clinical research projects of the hospital.Methods Collect relevant literatures from home and abroad;consult completion reports of various research projects completed from 2003 to 2010 and sum up problems found in such projects.Carry out in-depth interviews with principal investigators,supervisors of scientific research of the hospital as well as administrators of sponsor institutions.A questionnaire survey was made on completed clinical research projects undertaken from 2003 to 2010.Results Six key factors affecting implementation of such projects were derived from factor analysis,which are research technology and external environment,project teamwork,research subject availability,project organization,manpower input,and research design.A multiple logistic regression analysis found project internal management and manpower input as two leading predictive factors for project completion outcomes,while the former has a greater impact than the latter.Conclusion Project management should focus on the 6 key factors affecting project quality.The two main predictive factors should attract greater attention and resources in both supervision and management.

1.5). The mean intakes of riboflavin were 1.05 mg/day in the 2nd trimester and 1.03 mg/day in 3rd. The correlation of BGRAC value and riboflavin intake (riboflavin per day, ridoflavin per 1000 kcal/day) demonstrated a significant negative correlation. The cord blood BGRAC values were in normal range, indicating that placenta may transport riboflavin from mother to fetus actively. The mean riboflavin content of colostrum was 22.9ug/dl.

Objective To study the effects of calcitonin on bone mineral density(BMD), bone strength and bone fragile fracture in osteoporosis. Methods This is a one year, single centered, prospective and randomized, opening study: 135 patients with osteoporosis were divided into calcitonin+calcium group and calcium group. Patients of calcitonin+calcium group received salmon calcitonin(Miacalcic was injected intramuscularly, 50 IU/day in first week, 50 IU/two days in second week and 50 IU/twice/week later) and calcium(calcium 600 mg/day), patients in another group received calcium(calcium 600 mg/day). The determination markers included serum calcium, serum phosphate, serum alkline phosphatase, osteocalcin and urine hydroxyproline before and after treatment, at same time all patients were examined by DEXA(lumbar, hip) and QUS(radius, tibia). Results After 1 year treatment, 53 patients of calcitonin+calcium group and 59 patients of calcium group were followed up. In calcitonin+calcium group, BMD of lumbar spine increased about 1%(P

Objective To evaluate the value of Vascular Closure Device(VCD)with the new Angio-Seal STS(Self-Tightening Suture)in femoral artery closure after coronary angiography(CAG)or percutaneous coronary intervention(PCI).Methods Totally 298 patients were divided into Angio-Seal STS group(group A,n=120)and manual compression group(group B,n=178).The success rate of hemostasis,the time of hemostasis,the time of bedrest and the complication rate were observed.Results Compared with group B,the time of hemostasis,the time of bedrest and the rate of distress on the waist were markedly decreased in group A(P0.05).Conclusion Angio-Seal STS vascular closure derice provided a safe and effective means of obtainig rapid arterial hemostasis after cardiac catheterization.It reduces the bedrest time and discomfort without increasing complications.Angio-seal STS device has the feature of self tightening suture,and doesn't need the post-placement tension spring.

Objective To investigate the expression of poly (ADP-ribose) polymerase-1 (PARP-1 ), cytokeratins (CK) 7/20, and p53 in patients with Barrett's esophagus and esophageal adenocareinoma,and to evaluate their significance. Methods Expression of PARP-1, CK7/20 and p53 were determined by immunohistochemistry in 108 patients (including 40 Barrett's esophagus, 28 esophageal adenocarcinoma and 40 cardiac mucosa). Results The expression of PARP-1 was found in Barrett's esophagus, esophageal ade-nocarcinoma and cardiac epithelium with a significantly higher level in esophageal adenocarcinoma than the other two groups (P <0. 01 ). CK7/20 was expressed in much of intestinal metaplasia, part of cardiac epi-thelium and adenocarcinoma cells. The positive expression of p53 was observed in all three groups, and it was significantly higher in adenocarcinoma group than in other two groups (P < 0. 05 ). PARP-1 expression is highly correlated with that of p53 in Barrett's esophagus ( r= 0.49, P < 0.01 ). Conclusion CK7/20 is a sensitive but less specific indicator for intestinal metaplasia. Both PARP-1 and p53 are involved in the patho-genesis of esophageal adenocarcinoma and might help to determine the risk of Barrett's esophagus developing into esophageal adenocarcinoma.