Objective To observe the effect of speech therapy on the intelligence of children with developmental retardation of speech.Methods 20 cases of children with developmental retardation of speech were evaluated and individual language training program was made,especially trainings of abstract thought,logical thought and development of cognition ability of attention.All of the training contents were combined and make into play form.The training prolonged for 1～3 courses.Intelligence test and test of the level of language development were performed before and after the training.Results After training,10 children(50%) reached the general level,the total efficiency was 100%.Conclusion The multidisciplinary language training can improve intelligence,level of language development of children with developmental retardation of speech.

Objective To investigate the inhibition of Sophora flavescens extract on the degree of inflammatory swelling of guinea pigs'skin with eczema, observe pathological changes and explore the effects of the anti-in-flammatory action, in order to provide experimental basis for clinical treatment.Methods Eczema models of 40 guinea pigs were set up by smearing 2, 4-dinitrochlorobenzene on their napes to cause sensitization and motivating in the inside of their right ears.All guinea pigs were randomly divided into 5 groups equally,including the model com-pared group, positive control group and high, medium, low concentration groups of sophora flavescens.After eczema skin was continuously administered drugs for 14 days, the same part with equal area of the left and right ears was taken and weighed.The difference between the left and right ears was used to evaluate the swelling degree of skin inflammation.The pathological changes were observed by making pathological slices with HE staining with the tissue blocks of right ears.Results Compared with the model group, the swelling degree of skin inflammation was significantly relieved and dermal edema and inflammatory cell infiltrating were significantly improved in the high and medi-um concentration groups of sophora flavescens (p<0.05).Compared with the positive group, the swelling degree of skin inflammation, dermal edema and inflammatory cell infiltrating were significantly alleviated in the high concentra-tion group of sophora flavescens ( p<0.05) .Conclusion Sophora flavescens extract can effectively reduce inflam-matory response of guinea pigs'skin with eczema, having a certain significance for the clinical treatment of eczema.

Biofilm formation makes bacteria adopt a multicellular lifestyle.Compared to planktonic cells, biofilm-grown cells express an increased resistance to antimicrobial agents.Bacterial biofilm formation is a crucial step in the pathogenesis of many subacute and chronic bacterial infections.In clinical setting, biofilm can cause bacterial biofilm disease and biomaterial associated infection.Understanding the mechanisms involved in biofilm-associated antimicrobial resistance is key to the development of new solutions.

0.05),but all the outcome measures at 1 month in the treatment group were significantly improved as compared with control group(P0.05).The mean light sensitivity in the treatment group was better than in control group(P

ObjectiveTo analyze the significance of serum hyper sensitivity C-reactive protein (hs-CRP),lipoprotein (a) [ Lp(a) ] and total cholesterol (TC)/high density lipoprotein cholesterol ( HDL-C )in coronary heart disease.Methods From September 2010 to September 2011,the levels of serum hs-CRP,Lp (a) and TC/HDL-C were detected in 176 patients of coronary heart disease (coronary heart disease group ) and 60 healthy controls (control group).Coronary heart disease group included acute myocardial infarction with 56 cases,stable angina with 58 cases and unstable angina with 62 cases.ResultsThe levels of serum hs-C RP,Lp ( a ) and TC/HDL-C in coronary h cart disease group were (34.51 ± 9.65 ) mg/L,(295.16 ±104.57) mg/L and 4.23 ±0.91,while those in control group were (1.26 ±0.69) mg/L,(145.26 ±42.19)mg/L and 2.54 ± 0.57.There were significant differences between two groups (P ＜ 0.05 ).The levels of serum hs-CRP,Lp(a) and TC/HDL-C in acute myocardial infarction patients were significantly higher than those in unstable angina patients and stable angina patients (P ＜ 0.05).The levels of serum hs-CRP,Lp (a) and TC/HDL-C in unstable angina patients were significantly higher than those in stable angina patients(P ＜ 0.05).Conclusions Serum hs-CRP,Lp (a) and TC/HDL-C are significantly increased in coronary heart disease,and can reflex the severity of coronary heart disease.

BACKGROUND: Preplant diagnosis and rigorous implant strategy based on multislice spiral computed tomography (MSCT) imaging can improve success rate of mini-implants as anchorage. Effective application of imaging information in implant anchorage requires reliable tools and methods.OBJECTIVE: To investigate the accuracy of the radiographic and tunnel-type surgical templates assisting implant anchorage surgery and evaluate the clinical significance.METHODS: The transparent radiographic templates were made with occlusal rest to assist MSCT to scan two pigs' skulls locatively. After designed the implant anchorage angles in MSCT images accurately, we made the tunnel-type surgical template by angle data. A total of 32 miniscrews were implanted by surgical template, and the two pigs skulls were rescanned by MSCT. After dissected the pig alveolar process, the MSCT preoperative planning angles, the MSCT postoperative measurement angles and the measurement angles of anatomic bone samples were analyzed statistically. RESULT AND CONCLUSION: The 32 miniscrews were implanted successfully. They did not damage dental roots or surrounding important structures. Compared MSCT preoperative planning angles with MSCT postoperative measurement angles and anatomic bone samples measurement angles, there were no significant differences on axial sections or cross-sections and the r concordance correlations were very good. Results show that the radiographic and the tunnel-type surgical templates are accurate and reliable for operation and will help to improve the success rate of implant anchorage.

OBJECTIVE： To compare between two different modes of stability test for norgestrienone acetate.METHODS： HPLC(Resolve C18 column， 5μ m ID， 4.6mm× 250mm as fixed phase， a mixture of methanol and purified water (7∶ 3) as mobile phase， detecting wavelength 243nm， flow rate 1ml/min) and TLC.RESULTS： Two tests obtained the same result.CONCLUSION： The methods used are feasible for stability test.

Objective To explore the relationship between hepatic stem cells and hematopoietic stem cells by detecting the expression of OV6 and CD34 in rat hepatic oval cells. Methods Healthy SD rat were fed with chemical carcinogen 3’-me-DAB for 4 weeks, then the rat hepatic tissue samples were obtained. The rats fed with normal forage served as normal controls. The expressions of OV6 and CD34, specific markers for oval cells and hematopoietic stem cells respectively, in rat hepatic stem cells were detected by immunocytochemical staining (ABC method). Results The oval cells positive for OV6 and CD34 were found in the limiting plate of portal area, mainly located around the Hering canal. In addition, some oval cells positive for OV6 and CD34 were found migrating to hepatic lobule, scattering in the hepatic cords. A great many mononuclear stem cells positive for OV6 and CD34 were also found in the hepatic parenchyma. Conclusion 3’-me-DAB could stimulate the proliferation of hepatic oval cells and hematopoietic stem cells and cause the hematopoietic stem cells derived from bone marrow to migrate to hepatic parenchyma and differentiate.