Objective To establish an HPLC method to determine the two principal constituent-capsaicin and dihydrocapsaicin in general alkaloids of Capsaicin.Method The analysis was performed on C18 column(4.6 mm?250 mm,5 ?m),with methanol-water(70:30) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 280 nm.Result The linear relationships of capsaicin and dihydrocapsaicin were within the range of 1.955～7.820 ?g(r=0.999 9) and 2.709～7.224 ?g(r= 0.995 5),respectively.The average recoveries were 101.57% and 108.87%,respectively.Conclusion The method is convenient,the result is accurate and reliable,and can be used to determine the content of two principal constituent in general alkaloids of Capsaicin.

目的观察神经阻滞治疗颈源性头痛的效果。方法对颈源性头痛患者30例行星状神经节阻滞,伴颈椎旁神经或耳颞神经阻滞治疗,观察治疗前后疼痛视觉模拟评分(VAS)、每周头痛发作次数、服用止痛药物与睡眠改善例数。结果治疗前后VAS评分改善(P<0.05),治疗后头痛完全缓解15例,好转9例,有效4例,无效2例;治疗后头痛发作次数及持续时间均缩短(P<0.05);治疗前服用止痛药物28例,治疗后2周12例,4周4例(P<0.05);睡眠不佳治疗前29例,治疗后2周13例,4周为4例(P<0.05)。结论神经阻滞是治疗颈源性头痛是一种良好的治疗方法。

Behavior Science pays more attention to humanity,and regards the management of people as the key factor of management.Nursing management is the process targeting at the progress of nursing quality and work efficiency.Under the new circumstance,it is very important for the head nurse to supervise the nurses with a scientific management conception in the hospital howadays which will encourage the nurses to fully exert their abilities. and help to improve the nursing quality finally.

To achieve the goal of continuous improvement, measures of multi-link management were adopted for antimicrobial use, including establishing and improving organizational frameworks and rules and regulations, conducting various forms of training for all workers and staff, formulating criteria that were based on the frontier theories and could be easily applied, taking effective measures of intervention, using scientific evaluation methods, and getting timely feedback. The measures have proved to be effective in practice.

Objective:To construct a suppression subtractive library of suppression-related genes from c serotype Streptococcus mutans (S.mutans). Methods:After being isolated from virulent and avirulent strain of S. mutans respectively, the intact and high-pure genomic DNAs were digested with restriction enzyme AluⅠ. The digested DNA of the avirulent strain ligated with an adaptor was used as tester DNA, and that of the virulent strain as driver DNA. Then the suppression subtractive hybridization(SSH) was carried out, the efficiency of ligation and subtraction were detected respectively. The subtracted fragments were inserted into vector pCR2.1 using T/A cloning kit and transformed into E. coli TOP10F' competent cells. The white colonies were selected to construct the suppression subtractive library. Results: Through electrophoresis of AluⅠ-digested DNAs, a smear ranged from 0.1 to 2.0 kb was observed. The ligation efficiency of tester DNA with adaptor was at least higher than 25 percent. The subtraction efficiency confirmed the success in enrichment of differential genes between virulent and avirulent strain of S. mutans. In the subtracted group, the appearance time of the 23S rRNA gene in both tester and driver DNA was later than that in the unsubtracted group by twelve cycles. It suggested that suppression subtractive hybridization happened indeed. Then the subtracted fragments were cloned and the suppression-related gene library between virulent and avirulent strain of S. mutans was constructed. Conclusions:The suppression subtracted library of suppression-related genes has been constructed.

Objective:To explore avirulent strain-specific new genes or new functions of already known genes from Streptococcus mutans of serotype c.Methods:Twenty-six DNA fragments unique to avirulent strain of Streptococcus mutans were sequenced.The sequences of these presumptive avirulence DNA fragments were subjected to homology search through BLASTn and BLASTx in public database,and their putative biological functions were analyzed.Results:The size of the DNA fragments ranged from 113 bp to 776 bp.The average G+C content was 38.27%,similar to that of the gene-codingsequences in Streptococcus mutans strain UA159 whose genome sequences were just completed.Seven clones were picked repeatedly.Of the nineteen DNA fragments,eight potentially represented new DNA fragments were registered in GenBank.The remaining DNA fragments showed high homology to known genes of Streptococcus mutans strain UA159.Conclusions: The gene analysis and identification supply the groundwork for further study of gene functions of the avirulent strain of Streptococcus mutans serotype c.

ObjectiveTo observe the effects of local mild hypothermia at different time on dopamine (DA) and 5-hydroxytryptamine (5-HT) concentration in rabbits brain basal ganglion with bilirubin encephalopathy. MethodsThe model of bilirubin encephalopathy was established, and treated with mild hypothermia for different time. The DA and 5-HT in the basal ganglion were detected with fluorescence spectrophotometry. ResultsThe DA and the 5-HT decreased in the basal ganglion significantly after modeling (P<0.01), and increased when treated for 6 h, 12 h or 24 h (P<0.01). ConclusionLocal mild hypothermia can significantly reduce the disorder of DA and 5-HT during bilirubin encephalopathy. The longer the hypothermia, the better the effect is.

A method for the determination of caffeine in urea was developed based on bubble-in-drop single drop microextraction (BID-SDME) followed by gas chromatography/mass spectrometry (GC-MS).Under the optimum conditions including chloroform as extraction solvent, an exposure volume of 1 μL, a bubble volume of 1.6 μL, stirring for 5 min at 300 r/min, 15% (m/V) NaCl, and a distance of 1 cm between bubble and stirring bar, the detection limit of this method was as low as 0.003 mg/L and the linear range was from 0.005 mg/L to 10 mg/L with correlation coefficient of 0.982.The recoveries of caffeine were from 89.2% to 107.5% at different spiked levels in human urine and the relative standard deviation (RSD, n=6) was less than 8%.

Objective To study the function of LSD1 in the development of neurons and the influence of LSD1 on mood and memory-related behavior in mice. Methods The LSD1(flox / flox) transgenic mice were crossed with Nestin?cre(Tg) transgenic mice, using Cre?LoxP recombination system, to generate LSD1 conditional knockout of neural stem cell ( LSD1?CKO) mice, LSD1(flox/ flox) Nestin?cre(Tg) mice, and LSD1(flox/ flox) mice as control. The neuron proliferation in LSD1?CKO mice was further detected by immunofluorescence staining. At the same time, the mood and memory?related behavior of LSD1?CKO mice were examined using several methods:sucrose preference test ( SPT) , forced swimming test ( FST) and novel?object recognition ( NOR) assay. Results In the LSD1 brain?specific CKO mice, the neuron proliferation rate in the hippocampus was significantly reduced ( P=0. 023 ) , the preference for sucrose was reduced ( P =0. 0075 ) , immobility duration during the forced swimming test was increased (P<0. 05), and LSD1?CKO mice also exhibits memory?decline (P=0. 0019) during the novel?object recognition test. Conclusions Depletion of LSD1 in mouse brain neural stem cells leads to significant reduction of the neuron proliferation in the hippocampus. LSD1?CKO mice show more negative emotions and memory impairment.

OBJECTIVE:To establish a method for simultaneous determination of the confents of calycosin-7-glucoside,calyco-sin and formononetin in Shenqi granule. METHODS:HPLC was performed on the column of Welch Ultimate XB-C18 with mobile phase of acetonitrile-0.2% Formic acid solution (gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 250 nm, column temperature was 35 ℃,and injection volume was 10 μl. RESULTS:The linear range of sample quantity was 0.008-0.32 μg for calycosin-7-glucoside(r=0.999 9),0.005-0.20 μg for calycosin(r=0.999 7)and 0.007-0.28 μg for formononetin(r=0.999 8);RSDs of precsion,stability and reproducibility tests were no more than 1.59%;recoveries were 98.17%-99.17%(RSD=0.67%, n=6),98.03%-99.66%(RSD=0.57%,n=6) and 98.16%-99.12%(RSD=0.43%,n=6). CONCLUSIONS:The method is sim-ple,accurate and reliable,and can be used for the content determination of Shenqi granule ofiso flavones.