Objective:To study the relationship of circulating endothelial cell(CEC)with syndrome differentiation of TCM(Traditional Chinese Medicine)in patients with cardiovascular diseases(CD)and to observe the effect and clinical significance of CEC in the pathogenesis of CD.Methods:150 patients with CD were divided into four groups by syndrome differentiation of TCM.Besides,a control group consisted of 30 healthy persons.CEC was determined for all cases and healthy contral by Hladovec method.30 patients with coronary heart disease(CHD)accepted oral Tongxinluo capsule,3 capsules each time,Tid.The treatment course lasted one month.CEC was determined before and after treatment.Results:Compared with healthy group,the content of CEC in CD group increased obviously(P

Objective Artificial mineral water pills were made by loading many kinds of trace elements into the ceramic carrier.Pure water could be mineralized after dipping the pills into the water.To assess the possible hazardous effects on the human health induced by the pills,a series of hygienic and toxicological experiments on the pills were carried out.Methods Acute oral toxicity test,micronucleus test of mice bone marrow cells,sperm abnormality test in mice and Ames test were con-ducted.Contents of trace elements,heavy metals and radioactivity in the mineralized water were determined according to the examination methods in Standard Examination Methods for Drinking Water and Examination Methods for Drinking Natural Min-eral Water.Results The contents of trace elements,heavy metals and radioactivity in mineralized water met the requirements of GB5749-1985Sanitary Standard for Drinking Water and GB8537-1995Sanitary Standard for Drinking Natural Mineral Wa-ter.Mineral pills were classified as actually non-toxic and the results of micronucleus test of mice bone marrow cells,sperm ab-normality test in mice and Ames test were negative.Conclusion Mineralized drinking water treated by artificial mineral pills were safe.

Objective:To investigate the relationship of the changes of peripheral bloos T cell subsets and NK cell with non-Hodgkin's lymphoma(NHL).And to investigate the change of the cell immune function of NHL and chronic lymphadenitis.Methods:Peripheral blood T cell subsets,NK cell with NHL,chronic lymphadenitis patients and 50 normal controls were detected quantitatively with FCM.Results:Compared with the normal controls,the number of CD3~+,CD4~+T cell,CD4~+/CD8~+ ratio markedly reduced,while those of CD8~+T cells increased.These were no significant changes in NK cell.Compared with the group of chrnic lymphadenitis,the number of CD8~+T cell,NK cell markedly increased,while these were no significant changes in CD3~+,CD4~+T cell and reduction of CD4~+/CD8~+ ratio.Conclusion:NHL patients cell function was suppressed.The detection of T cell subsets and NK cells are clinically valuble on the diagnosis,treatment and prognosis of NHL.

The medical student should learn the knowledge and skill for NI control.Nosocomiology subject should be set up in higher medical education as early as possible to meet the needs of medical science development.

Objective To investigate the value of CT virtual endoscopy(CTVE) and multiple plane(reconstruction)(MPR)for preoperative staging of rectal cancer.Methods Forty-five patients with rectal(cancer) were examined by CTVE and MPR to determine the preoperative staging,and the reselts were compared with CT.Results For evaluation of intramural invasion,the accuracy rate of CTVE and MPR was 86.7%(39/45),pelvic CT was 66.7%(30/45)(P

OBJECTIVE To investigate the pertinency between acute physiology and chronic health evaluation Ⅱ scores(APACHEⅡ)and nosocomial infection.METHODS The clinical data from 1 007 ICU patients were evaluated with APACHEⅡ scores system,and made a prospective survey of nosocomial infection.RESULTS Along with the increase in APACHEⅡ scores,nosocomial infection rate and mortality showed rising trend and they were in linear relationship.CONCLUSIONS APACHEⅡ scores system not only can appraise the degrees of severity of illness,evaluate medical treatment,and estimate the prognosis,but also forecast nosocomial infection.

Objective To study the protective effect of Xinfuli Granula(XG) on acute and chronic cardiac failure in cats and rats. Methods The cat models of acute cardiac failure were established by injecting pentobarbital. Then the cat models were given XG through duodenum. After medication,hemodynamics parameters such as heart rate(HR),mean arterial pressure(MAP),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),+ dp/dtmax and cardiac output(CO) in cats were determined. The rats were divided randomly into 5 groups:the control group,the model group,the positive control group,and high-dose and Low-dose XG groups. The rat models of myocardial injury were induced by isoproterenol(20 mg/kg). The normal group and the model group were treated with normal saline(10 mL/kg) orally,the positive contral group were treated with cedilanid(0.2 mg/kg) by intravenous,the high-and low-dose XG groups were orally treated with XG(10 g/kg and 5 g/kg respectively),and the propranolol group was orally treated with propranolol(4 mg/kg),once a day for successive 8 days. Animals were sacrificed on the 9th day,then the heart was taken out and the cardiac index was calculated. The pathological changes in myocardial cells of the rats were observed under electron microscope. Results XG had no effect on hemodynamics of cats with acute cardiac failure,but had obvious protective and therapeutic effect on isoproterenol-inducedmyocardial injury and myocardial ischemia of rats. Conclusion Xinfuli Granula has good preventive effect on isoproterenol-induced myocardial injury and sub-acute cardiac failure of rats,but has no effect on acute cardiac failure of cats.

AIM: To compare two methods of HPLC-MS/MS and MEIA to monitor the tacrolimus concentrations for organ transplantation patient.METHODS: After developing and validating the HPLC-MS/MS method to determine the concentrations of tacrolimus,the tacrolimus samples were quantitated by two methods of HPLC-MS/MS and MEIA,respectively.Evaluate two methods by statistical analysis of quantitative results.RESULTS: The mean concentration were(4.86?0.46) ng/mL by HPLC-MS/MS and(5.52?0.43) ng/mL by MEIA.The coefficient correlation of two methods was 0.8771,and the two methods have good correlations in statistics.The mean concentration ratio was(90.3?5.3)%.CONCLUSION: The HPLC-MS/MS method is a more precise method for to determining the effective concentration of tacrolimus,which is suitable for daily TDM.

Objective To explore the investigation and control method for the epidemic of surgical site infection (SSI)in a hospital.Methods Data of orthopedics patients receiving clean wound orthopedics operation were ana-lyzed retrospectively,and effective measures were taken to control the epidemic of HAI.Results From June to August 2012,a total of 312 patients received clean wound orthopedics operation,7 (2.24%)SSI cases occurred.SSI rates during the same period from 2009 to 2012 were significantly different(P <0.05),and was the highest in 2012. HAI management staff reviewed patients’medical records,communicated with staff in orthopedics department,and put forward advice for SSI control;orthopedics department worked out and implemented detailed infection control measures.The subsequent surveillance showed that the prevalence of SSI in orthopedics ward decreased to 0.38%, infection outbreak was effectively controlled.Conclusion HAI management department played an important role in the finding and control of HAI outbreak.Scientific analysis on monitored data and proper measures adopted by HAI management department and clinical department can effectively control HAI outbreak.