Objective:To study the relationship of circulating endothelial cell(CEC)with syndrome differentiation of TCM(Traditional Chinese Medicine)in patients with cardiovascular diseases(CD)and to observe the effect and clinical significance of CEC in the pathogenesis of CD.Methods:150 patients with CD were divided into four groups by syndrome differentiation of TCM.Besides,a control group consisted of 30 healthy persons.CEC was determined for all cases and healthy contral by Hladovec method.30 patients with coronary heart disease(CHD)accepted oral Tongxinluo capsule,3 capsules each time,Tid.The treatment course lasted one month.CEC was determined before and after treatment.Results:Compared with healthy group,the content of CEC in CD group increased obviously(P

Objective Artificial mineral water pills were made by loading many kinds of trace elements into the ceramic carrier.Pure water could be mineralized after dipping the pills into the water.To assess the possible hazardous effects on the human health induced by the pills,a series of hygienic and toxicological experiments on the pills were carried out.Methods Acute oral toxicity test,micronucleus test of mice bone marrow cells,sperm abnormality test in mice and Ames test were con-ducted.Contents of trace elements,heavy metals and radioactivity in the mineralized water were determined according to the examination methods in Standard Examination Methods for Drinking Water and Examination Methods for Drinking Natural Min-eral Water.Results The contents of trace elements,heavy metals and radioactivity in mineralized water met the requirements of GB5749-1985Sanitary Standard for Drinking Water and GB8537-1995Sanitary Standard for Drinking Natural Mineral Wa-ter.Mineral pills were classified as actually non-toxic and the results of micronucleus test of mice bone marrow cells,sperm ab-normality test in mice and Ames test were negative.Conclusion Mineralized drinking water treated by artificial mineral pills were safe.

Objective:To investigate the relationship of the changes of peripheral bloos T cell subsets and NK cell with non-Hodgkin's lymphoma(NHL).And to investigate the change of the cell immune function of NHL and chronic lymphadenitis.Methods:Peripheral blood T cell subsets,NK cell with NHL,chronic lymphadenitis patients and 50 normal controls were detected quantitatively with FCM.Results:Compared with the normal controls,the number of CD3~+,CD4~+T cell,CD4~+/CD8~+ ratio markedly reduced,while those of CD8~+T cells increased.These were no significant changes in NK cell.Compared with the group of chrnic lymphadenitis,the number of CD8~+T cell,NK cell markedly increased,while these were no significant changes in CD3~+,CD4~+T cell and reduction of CD4~+/CD8~+ ratio.Conclusion:NHL patients cell function was suppressed.The detection of T cell subsets and NK cells are clinically valuble on the diagnosis,treatment and prognosis of NHL.

Objective To explore the investigation and control method for the epidemic of surgical site infection (SSI)in a hospital.Methods Data of orthopedics patients receiving clean wound orthopedics operation were ana-lyzed retrospectively,and effective measures were taken to control the epidemic of HAI.Results From June to August 2012,a total of 312 patients received clean wound orthopedics operation,7 (2.24%)SSI cases occurred.SSI rates during the same period from 2009 to 2012 were significantly different(P <0.05),and was the highest in 2012. HAI management staff reviewed patients’medical records,communicated with staff in orthopedics department,and put forward advice for SSI control;orthopedics department worked out and implemented detailed infection control measures.The subsequent surveillance showed that the prevalence of SSI in orthopedics ward decreased to 0.38%, infection outbreak was effectively controlled.Conclusion HAI management department played an important role in the finding and control of HAI outbreak.Scientific analysis on monitored data and proper measures adopted by HAI management department and clinical department can effectively control HAI outbreak.

OBJECTIVE To focus on the diagnosis,treatment and prevention of central venous catheter(CVC)-related bloodstream infections(CRBSI)in clinical practice.METHODS The related articles within the nearest 10 years were reviewed,which published in the domestiec and foreign academic journals,at the same time,the related monitoring data of the hospital were analyzed.RESULTS Highlights on the diagnosis,treatment and prevention of CRBSI:for the patients with CVC,the possibility of the CRBSI should be considered,as soon as a high fever was found,and it could not be explained with the non-infectious causes.Infection occurring time and pathogen were different between the general catheters and the anti-infective catheters.The effect of the anti-infective catheters was limited.To pull out the catheters was one of the most effective treatment measures.CONCLUSIONS Prevention of CRBSI:the indications of CVC intubation should be undisputed.Silicone catheter should be used.When the catheter is predicted to be indwelling more than 5 days,anti-infective catheters should be chosen.To insert CVC at the subclavian vein instead of the femoral vein.To care the hub and keep the puncture point dry and do disinfection well.The health care workers should be trained.

Objective To study the extracellular signal-regulated kinase 1/2(ERK1/2) expression of renal cells in epilepsy rats induced by kainic acid,and elucidate the mechanism of renal injury caused by epilepsy.Methods 70 male Wistar rats were randomly divided into two groups:epilepsy group and control group,35 rats in each group.Rat epilepsy model was prepared by injection of kainic acid into amygdala under three-dimensional positioning devices.At different time points(0,2,6,12 and 24 h) after epilepsy spasm,the renal tissue specimens were immediately prepared for immunohistochemistry.The ERK1/2 expression of rat renal cells was calculated and compared with control group.Results The ERK1/2 expression(gray value) of renal tubular epithelial cells in epilepsy rats began to increase gradually after epilepsy spasm.It reached peak at 6 h after epilepsy spasm.The ERK1/2 expression of renal tubular epithelial cells in epilepsy rats was significantly higher than at 0 h(P0.05).There were no major pathological changes in kidney of rats with epilepsy stained with HE.Conclusion ERK1/2 activation may have important role in the renal injury caused by epilepsy.Inhibition of the activation of ERK1/2 may lead to the renal protection.

OBJECTIVE To evaluate the risk factors of nosocomial infection(NI),then establish the evaluation system for risk factors of NI in ICU.METHODS A total of 1897 ICU patients were enrolled in our study.The risk factors of NI were classified and quantified,then investigated the relativity of the risk factors and NI.RESULTS NI correlated with APACHEⅡscore,serum prealbumin level,blood products uese,incision of trachea,mechanical ventilation,central vein cannula and enteral nutrition.CONCLUSIONS The risk factors of NI evaluatied in ICU can to be used to lay the foundation for the model of NI early warning.

OBJECTIVE To study the risk factors of hospital acquired urinary system infection in neurology ICU(NICU) and emergency ICU(EICU).METHODS The general character,score of APACHE Ⅱ and the time length of urethral catheterization of the 502 NICU and EICU patients were enrolled in our study.Then the relativity of the risk factors and hospital acquired urinary system infection were investigated.RESULTS Urethral catheterization increased the chances of hospital acquired urinary system infection and there were more chances with the long time.CONCLUSIONS In order to decrease the rate of hospital acquired urinary system infection we must strictly grasp the indication of urethral catheterization and pull out the urethral catheter as early as possible.

OBJECTIVE To study relativity of systemic inflammatory response syndrome(SIRS) score and nosocomial infection(NI) and outcome in ICU patients.METHODS A total of 1897 ICU patients were enrolled in our study.The SIRS within 48 hours after admission was recorded.And the prospective evaluation in NI and prognosis was made.RESULTS The nosocomial infection rate was 10.1%,and the mortality was 9.9%.With SIRS score increasing,NI and mortality rates increased as well and the tendency presented a linear correlation.CONCLUSIONS As a simple system,SIRS score can initially predict the risk of NI and outcome in ICU patients.

OBJECTIVE To investigate the pertinency between acute physiology and chronic health evaluation Ⅱ scores(APACHEⅡ)and nosocomial infection.METHODS The clinical data from 1 007 ICU patients were evaluated with APACHEⅡ scores system,and made a prospective survey of nosocomial infection.RESULTS Along with the increase in APACHEⅡ scores,nosocomial infection rate and mortality showed rising trend and they were in linear relationship.CONCLUSIONS APACHEⅡ scores system not only can appraise the degrees of severity of illness,evaluate medical treatment,and estimate the prognosis,but also forecast nosocomial infection.