Objective To investigate angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism in patients with hypertension in the elderly and it's relation with serum ACE level among Chinese population.Methods Polymorphism of ACE gene was categorized into three genotype:two deletion alleles (genotype DD),heterozygous alleles (genotype ID),and two insertion alleles (genotype II).Polymerase chain reaction(PCR) was used to examine ACE gene polymorphism.Compared the distribution of three ACE genotypes frequencies and D allele frequency in 56 patients with hypertension in the elderly and 40 healthy subjects,whose serum ACE levels were measured.Results The frequency of D allele was higher in the hypertension group (0.61) than in healthy subjects (0.46) (P<0.05).A significant difference in serum ACE level was observed between the hypertensives and healthy (31.0±11.1) U/L and (25.1±10.0) U/L respectively,P<0.05.The highest level of serum ACE activity was in genotype DD,and the lowest level in genotype II,and intermediate in genotype ID.A significant difference in serum ACE level was observed among the genotype DD and ID,and among the genotype DD and II.Conclusion ACE gene I/D polymorphism was associated with serum ACE level.D allele might be a genetic risk factor for hypertension in the elderly.

Objective To investigate the hemodynamics of basilar artery and its blood-supply area in patients with vertigo by 3.0 T MR.Methods Fifty patients with vertigo were selected as case group and 50 healthy volunteers were selected as control group.All subjects in the case group and in control group were examined by brain magnetic resonance imaging (MRI),magnetic resonance angiography(MRA),fast cine phase contrast(Fast CINE PC) and flow-sensitive alternating inversion recovery sequence (FAIR).After image postprocessing,the velocity and the blood flow volume of basilar artery were measured and calculated.The FAIR value of bilateral occipital lobe,cerebellum,pons and medulla oblongata was measured.Statistical testing was performed.Results The average velocity,peak velocity and blood flow volume of the basilar artery in case group were significantly lower than those in the control group(the average velocity:(22.47 ±9.14) cm/s vs (31.41 ±5.97) cm/s,t =-5.79,P ＜ 0.0001 ; peak velocity:(31.60 ± 13.18) cm/s vs (44.00 ± 7.84) cm/s,t =-5.71,P ＜0.0001 ;blood flow volume:(91.14 ±43.92) ml/min vs (127.49 ± 28.33) ml/min,t =-4.92,P ＜0.0001).The perfusion of the posterior circulation in the case group was lower than that in the control group ((882.35 ±35.22) vs (906.34 ±36.82),t =-3.33,P =0.0012).The average velocity,peak velocity and blood flow volume of the basilar artery in case group whose brain parenchyma and blood vessels were normal were (26.31 ±5.16) cm/s,(36.33 ±6.60) cm/s,(95.38 ±28.16) ml/min respectively,which were all less than those in the control group (average velocity:t =-2.72,P =0.0086 ; peak velocity:t =-3.12,P =0.0027 ;blood flow volume:t =-3.53,P =0.0008).The perfusion of the basilar artery blood-supply area in case group whose brain parenchyma and blood vessels were normal was (904.00 ± 35.82),there was no difference when compared with the control group (906.00 ± 36.82,t =-0.20,P =0.8427).Conclusion Whether the brain parenchyma and blood vessels is normal or not,the velocity and blood flow volume of the basilar artery in patients with vertigo are decreased,but the perfusion of the basilar artery blood-supply area are different,it depends on whether there is ischemia.

Proprotein convertase subtilisin/kexin 9 (PCSK9) inhibitor is recently added to the list of effective lipid-lowering drugs in addition to statins, which can reduce low density lipoprotein cholesterol, treat increased low density lipoprotein cholesterol (LDL-C) and atherosclerotic cardiovascular disease (ASCVD) patients. In the past 30 years, the prevalence of dyslipidemia increased significantly. LDL-C characterized dyslipidemia is an important risk factor of ASCVD and a part of metabolic syndrome, which exists before or after or at the same time of the appearance of obesity, diabetes, and glucose intolerance, coronary heart disease and other diseases. Decreasing LDL-C level can significantly reduce the incidence and mortality risk of ASCVD. In this paper, the research on the regulation of dyslipidemia by PCSK9 inhibitors is discussed.

Objective To investigate the therapeutic efficacy of coronary heart disease intervention and effect on vascular endothelial growth factor(VEGF) and C-reactive protein(CRP) levels.Methods 127 cases with angina and myocardial infarct were done PCI(PTCA/PTCA percutaneous transcoronary angioplasty+stenting),the therapeutic efficacy was observed,and perioperative VEGF and CRP levels were determined.Results 146 brackets cases were placed in 127 cases,all brackets were successfully inserted into target vascular lesion sites;2 cases with two rami pathological changes given up PTCA because guide wire couldn't pass anterior descending branch narrow part;4 cases took place no re-flow phenomenon,operation achievement ratio was 95.3%.The levels of post-operative CRP increased 142.9%,but the levels of post-operative VEGF decreased 31.9%.Conclusion The therapeutic efficacy PCI on coronary heart disease is satisfactory monitoring CRP and VEGF in blood serum could be helpful to understand the therapeutic efficacy and inflammation degree.

Objective To investigate the association between the insertion/deletion(I/D) polymorphism of angiotensin converting enzyme (ACE) gene and the myocardial infarction (MI) among Chinese population. Methods Polymerase chain reaction(PCR) was applied to the examination of ACE gene polymorphism. A comparison was performed between 50 patients with MI and another 50 healthy subjects.Results The frequencies of DD genotype (0.38) and D allele (0.58) were both higher among the MI group than that among the control group (0.16 and 0.41 respectively,P

Objective To study possible mechanisms of oxidative damages in rat lung type Ⅱ cells induced by cooking oil fume (COF). Methods The rat type Ⅱ lung cells were pretreated with anti_oxidant N_acetylcysteine (NAC) for 30 miuntes and were exposed to COF for 12 hours. The contents of MDA and GSH were detected with thiobarbituric acid colorimetric assay and 5,5_dithiobis 2_nitrobenzoic acid colorimetric assay. Results The significant increase of the contents of MDA and significant decrease of the contents of GSH were observed in the rat lung type Ⅱ cells with the increase of exposure doses of COF and the prolongation of exposure time of COF. The pretratment of NAC could reduce the production of MDA and increase the contents of GSH of the cells. Conclusion The possible mechanisms of oxidative damages in rat lung type Ⅱ cells induced by cooking oil rume might be formation of lipid peroxidation and interference of GSH anti_oxidative systems of the cells.

Objective To explore the relationship of the level of serum selenium and infantile diarrhea,provide foundation for establishing the therapeutic criteria.Methods Seventy-eight diarrhea children was enrolled in this study,6 -24 months old.Thirty children with acute diarrhea (AD group),26 children with persistent diarrhea (PD group) and 22 children with chronic diarrhea (CD group).The level of serum selenium was measured and compared with another 30 healthy children (control group) of matched sex and age.The level of serum selenium of CD group was compared before and after the recovery.Results The level of serum selenium in AD group and PD group had no significant difference compared with control group [ (51.34 ± 4.84),(48.14 ± 3.05 ) μ g/L vs.(55.08 ± 5.59 ) μ g/L ] (P＞0.05 ).But the level of serum selenium in CD group was significantly lower than that in control group [ (42.13 ± 5.16) μ g/L vs.(55.08 ±5.59) μg/L] (P＜0.05).After treatment for 1 month,the level of serum selenium in CD group was significantly increased than before treatment [ (53.76 ± 8.38 ) μ g/L vs.(42.13 ± 5.16) μ g/L ] (P＜0.05 ).Conclusions The nosogenesis of chronic diarrhea may relate with the level of serum selenium decrease.Therapeutic selenium supplement is important in children with chronic diarrhea.

Objective To observe the clinical effect of the treatment of bronchial asthma with the Qingfeiyin and Bailing capsule.Methods 107 cases with bronchial asthma were classified into a control group and a treatment group randomly.The control group was treated with the Ipratropine (40-80 ug for each time)and the treatment group was glven Ipratropine and Qingfeireyin(50ml for each time)plus Bailing Capsule(30pills for each time).One course of treatment was 2months.Results The therapeutic effect of treatment group was significantly better than the control group(P＜0.05).Condusion Qingfeiyin and Bailing capsule has excellent effect in treating bronchial asthma.

Objective:To analyze influence factors in patients with acute myocardial infarction (AMI) undergoing e‐mergency percutaneous coronary intervention (PCI ) .Methods:Clinical data of 656 patients ,who received emer‐gency PCI because of AMI in our hospital from Jan 2005 to Jan 2015 ,were retrospectively analyzed .Short -term prognosis ,including incidence rates of heart failure (HF) ,cardiogenic shock (CS) ,arrhythmias and mortality , were compared and analyzed among these patients from onset related factors ,coronary disease and vascular reope‐ning time etc .Results:Mean onset age of men was significantly younger than that of women (P<0.05) .Incidence rate of AMI in winter and spring was significantly higher than those in summer and autumn (χ2 = 6.244 , P=0.012) .According to complicated with hypertension ,DM ,dyslipidemia and smoking or not ,patients were divided into corresponding disease group and normal group ;only incidence rates of CS (37.78% vs .29.62% ) and arrhyth‐mia (47.78% vs .38.24% ) in DM group were significantly higher than those of non -DM group ,mortality rate of dyslipidemia group was significantly lower than that of normal blood lipid group (0.73% vs .3.69% ) , P<0.05 or< 0.01 ,there were no significant difference in incidence rates of other events between normal group and disease group (P>0.05 all) .Incidence rate of arrhythmia in RCA group was significantly higher than those of LAD group , LCX group and multi -vessel group (56.36% vs .31.55% ,37.50% ,34.38% ) ,mortality of LM group was signifi‐cantly higher than those of LAD group ,RCA group and LCX group (25.00% vs .1.79% ,0.91% ,0% ) , P<0.05 or <0.01. Incidence rate of HF in 10~12h group was significantly higher than those of 0~3h ,4~6h and 7~9h group (79.46% vs .61.70% ,66.81% ,64.78% ) ,incidence rate of arrhythmia was significantly lower than those of 0~3h ,4~6h and 7~9h group (32.14% vs .55.32% ,43.81% ,44.65% ) ,and incidence rate of CS was significant‐ly higher than that of 0~3h group (35.27% vs .21.28% ) , P<0.05 or <0.01. Conclusion:Onset age ,season and DM ,coronary disease extent ,vascular reopening time are risk factors influencing short‐term prognosis of AMI .

OBJECTIVE To investigate the clinical distribution and resistance of Acinetobacter baumannii.METHODS The bacteria were identified by BioMerieux VITEK32.Susceptibility was tested by Kirby-Bauer method.RESULTS Among specimens from which A.baumannii was examined,sputum accounted for 78.64%,secretion(9.62%)and urine(6.81%).A.baumannii was mainly distributed at ICU ward(29.58%)and then was respiratory ward(21.36%).The resistance of A.baumannii was serious.The antibiotic drugs which had the low resistance were ?-lactam/?-lactamase inhibitor combinations such as sulperazone(22.14%)and carbapenems such as meropenem(32.95%),imipenem(33.04%),and so on.CONCLUSIONS The infecton of multi-drug resistant A.baumannii has increasing tendency.Therefore monitoring A.baumannii constantly and regularly,finding out resistant strains timely and adjusting the treatment methods are very important for the prevention of nosocomial infection.