Objective To observe the effect of team-based continuous nursing on improving the life function of children with cerebral palsy. Methods From March 2014 to March 2016, 35 children aged 18-36 months with cerebral palsy were selected as the experimental group;35 matched patients with the same age, same gender, same Gross Motor Function Classification System (GMFCS) and functionally similar were selected as the control group by matching design. Among them, 32 cases were followed up in the experimental group while 30 cases in the control group. In the control group, routine discharge education was adopted, but the experimental group was treated by team-based continuous nursing. The ability of daily living was evaluated by the Pediatric Evaluation of Disability Inventory (PEDI). Neuropsychological development was evaluated by the Gesell Development Scale and Body balance was evaluated by the Berg Balance Function Scale after discharge and also after 6 months follow-up. Results After the intervention for 6 months, the functional skill and assisting assistance of PEDI was (77.56 ± 23.58), (38.78 ± 13.96) points in the experimental group, and (65.12 ± 24.23), (30.35 ± 12.78) points in the control groups, there were significant differences between two groups(t=2.0485, 2.4750, P<0.05). The scores of Gesell Development Scale was (76.12 ± 18.35) points in the experimental group, and (71.85 ± 18.46) points in the control group, there were no significant differences between two groups(P>0.05) , but the experimental group was higher than the control group. The scores of Berg Balance Function Scale was(33.20 ± 4.12) points in the experimental group, and (29.67 ± 4.98) points in the control group, there were significant differences between two groups (t = 3.0488, P<0.05). Conclusions To improve the life function of children with cerebral palsy, the implementation of the team-based continuous nursing had a significant effect.

OBJECTIVE To strengthen the management of medical refuse in order to control the onset of hospital(infection.) METHODS It has been done including setting up special tissue,formulating supervising system,strengthening training,carrying out supervising measures and strengthening examination and inspection.(RESULTS) It could prevent hospital infection effectively to regulate the whole course of the medical refuses(collection,) storage,dealing with and registration.CONCLUSIONS It is very important to strengthen the management of medical refuse in order to prevent hospital infection.

AIM: To observe the effect of mineral trioxide aggregate (MTA) combined with fibronectin as pulp capping agents. METHODS: The 48 pulps of 4 cats were exposed mechanically and then capped directly with MTA, MTA combined with fibronectin, calcium hydroxide, fibronectin and starch as control. After 12 weeks observation, the experimental teeth were extracted and observed with scanning electronic microscope. RESULTS: In calcium hydroxide group and MTA group, specimens showed irregular dentin bridge structures along the pulp-MTA interface. In MTA combined with fibronectin group, continuous and complete dentin bridge structures were found along the pulp-MTA interface, the crystalline-like structures were in direct contact with the dentin wall of pulp chamber, the exposed pulp sites were closed by the dentin bridge. In fibronectin and starch group, no reparative dentin was observed. CONCLUSION: The present experiment indicated that MTA combined with fibronectin was an effective pulp-capping material and may have potential for clinical application. [

Objective:To observe the clinical effect of acupuncture therapy to support yang and consolidate the constitution for cervical spondylosis of vertebral artery type (CSA). Methods:A total of 72 patients with CSA were randomly divided upon the random digital table into an acupuncture- moxibustion group and an acupuncture group, 36 cases in each group. The acupuncture-moxibustion group was treated by electroacupuncture (EA) at bilateral Fengchi (GB 20) and C4-6 Jiaji (EX-B 2), in combination of moxibustion at Baihui (GV 20), Shenshu (BL 23) and Mingmen (GV 4) to support yang and consolidate constitution. The acupuncture group was given by routine EA at bilateral Fengchi (GB 20), cervical Jiaji (EX-B 2), Tianzhu (BL 10), Jianjing (GB 21), Houxi (SI 3), Hegu (LI 4) and Waiguan (TE 5). The treatment was given once every day and continuous six sessions made one course. After 2-course treatment, the improved situation of clinical symptoms and clinical effect were observed in the two groups. Results:The total effective rate was 91.7% in the acupuncture-moxibustion group and 61.1% in the acupuncture group. The difference of the total effective rate between the two groups was statistically significant (P<0.05). After treatment, the scores of the clinical symptoms in the two groups were obviously decreased than those of the same group before treatment (P<0.01). The differences in the scores between the two groups were statistically significant (P<0.05). Conclusion:Acupuncture-moxibustion treatment to support yang and consolidate constitution is better than the routine acupuncture therapy in the therapeutic effect for CSA.

Objective To observe the effect ofDanhuai-Yinxie condensed pill on the expression of VEGF and its receptors in the model rabbits with psoriasis.Methods Fourty-two healthy Japanese white rabbits were randomly divided into the control group, the model group, the Acitretin group, and the low-, medium- and high-doses ofDanhuai-Yinxie condensed pill groups. The backs of rabbits’ ears were wiped with 5% propranolol for 4 weeks in the latter four groups, while with saline for the same period in the control group. The rabbits in the Acitretin group were intragastric administrated with 1% Acitretin one day since the models of psoriasis were successed, while the rabbits in the low-, medium- and high-doses ofDanhuai-Yinxie condensed pill groups were intragastric administrated withDanhuai-Yinxie suspension 20, 40, 80 mg/kg daily, respectively. The rabbits in the control and model groups were intragastric administrated with equal-volume saline daily. The expression of VEGF, its receptor 1 (FLT-1) and receptor 2 (KDR) were detected by ELISA method, and the expression of VEGF mRNA was detected by PCR method, dermal papillary vascular density (MVD) and the T lymphocytes proliferation from peripheral blood were detected.Results The expression of VEGF (114.43 ± 952 ng/ml, 109.65 ± 9.15 ng/mlvs. 179.90 ± 11.30 ng/ml), VEGF mRNA (0.83 ± 0.07, 0.80 ± 0.06vs. 2.74 ±  0.15), FLT-1 (4.54 ± 0.26 ng/ml, 4.16 ± 0.24 ng/mlvs. 6.92 ± 0.38 ng/ml), KDR (2.48 ± 0.16 ng/ml, 2.51 ± 0.19 ng/mlvs. 6.79 ± 0.27 ng/ml), MVD (10.12 ± 1.97, 9.83 ± 1.12vs. 16.45 ± 2.05) and T lymphocytes proliferation (87.35% ± 6.29%, 86.21% ± 6.42%vs. 123.74% ± 9.18%) in the medium- and the high-dose groups significant decreased than those in the model group (P<0.05).ConclusionDanhuai-Yinxiecondensed pill could reduce the expression of VEGF, block the binding of VEGF to its receptors, inhibit angiogenesis, inhibit the reconstruction of subcutaneous tissues, reduce the dysplasia of dermal microvessels, and inhibit the proliferation of T cells.

Objective To study the role of anti-HLA-IgG antibodies levels in serum before renal transplantation.Methods Anti-HLA-IgG antibodies levels were detected by means of ELISA-LATM in 184 patients before renal transplantation from Jan. 1999 to Dec 2002. Acute rejection occurrence was compared between anti-HLA-IgG class-Ⅰantibody positive group and anti-HLA-IgG class-Ⅱantibody positive group, and between both class-Ⅰand class-Ⅱantibody positive groups and anti-HLA-IgG antibody negative group, respectively. Results In the 155, 7, 9 and 13 cases respectively negative for Anti-HLA-IgG antibodies, positive for anti-HLA-IgG class-Ⅰantibodies, anti-HLA-IgG class-Ⅱantibodies and both class-Ⅰand class-Ⅱantibodies, there were 18, 2, 4, 8 cases of acute rejection with the occurrence rate being 11.6?% , 28.57?% , 44.44?% , 61.53?% , respectively ( P

We carried out the stress relaxation and creep experiments on the aorta ascendens of five fresh dead bodies of normal chinese men who had died from impetuous trauma on the head. The stress relaxation and creep data and curves of the aorta ascendens were obtained. Then regression analysis was performed to get the reduced stress relaxation and creep functions. Finally, we analyzed and discussed the results of the experiment.
Adult ; Aorta ; physiology ; Elasticity ; Humans ; In Vitro Techniques ; Male ; Regression Analysis ; Stress, Mechanical ; Viscosity

Objedctive To study the mechanism of GnRH agalogus(Alarelin)on[ Ca2 + ]; mobilization in stomach smooth muscle cells (SSMc)of rats. Methods Cells were cutured and loaded with Fluo-3-AM. [Ca2 + ]; was measured by nuomeent intensity(FL) in each cell with confocal micoopy. Results (1 ) In Hanks solution. l0-7, l0-6, l0 - 5mol L- 1 Alarelin can elevate[ Ca2 + ], its peak - resting values reached 6. 00 ?0. 50 .9. 23 ?0. 62. l8. 97 ?2. 42 respectively, which indicate that the level of [Ca2+ ]; act in an deede- pendent and time - dependent. (2) thntredly. when Alarelin was 10 4mol?Ｌ－１, its peak - resting value only reached 6. 32 ?0 .67, conpared with Alarelin l0 5m.l L－1 which was sigificantly lower(P 0. 05 ). (5 )When pertreatment with Lacidipine in Hanks solution, the effect of Alarelin l0 5mol L-1.as partly inhibited(P

To study the bioequivalence of a kind of progesterone sustained release suppository, a randomized cross-over study was conducted in 12 rabbits. A single rectal dose of 2.75 mg/kg progesterone sustained released suppository (tested formulation, T) and progesterone suppository (reference formulation, R) was administered; a multiple dose of 2.75 mg/kg was given up to seven times with an interval of 8 h. Concentrations in serum were determined by a competitive enzyme immunoassay. The main parameters of T were: for single and multiple doses, Cmax was 48.8 +/- 11.8 ng/mL and 43.5 +/- 9.4 ng/mL, Tmax was 0.5 +/- 0.3 h and 0.4 +/- 0.3 h, AUC(0-24 h) was 362.4 +/- 143 ng x h x mL(-1) and 310.6 +/- 70.3 ng x h x mL(-1), respectively. The relative bioavailability of T to R were (104.2 +/- 13.4)% and (111.4 +/- 19.1)%, respectively. Statistical analysis showed that the two formulations were bioequivalent and T had sustained released feature.
Administration, Rectal ; Biological Availability ; Cross-Over Studies ; Progesterone/administration & dosage ; Progesterone/*pharmacokinetics ; Random Allocation ; Suppositories

Objective To investigate the influence of systematic health education on the disease cogni-tion and behavior of treatment compliance in patients with infectious diseases. Methods Patiens with infec-tious diseases in our department were divided into the control group(142 patients) and the experimental group (136 patients). The control group received common health education, the experimental group was given addi-tional knowledge education about infectious disease based upon routine education. The two groups were inves-tigated before and after intervention by adopting self-designed investigation scale.The cognition rate of disease and the rate of behavior of treatment compliance were compared by χ2 test. Results The cognition rate of disease and the rate of behavior of treatment compliance in the experimental group were higher than those of the control group. Conclusions Systematic health education can not only increase degree of disease cogni-tion but also improve behavior of treatment comphanee in patients with infectious diseases.