Objective To explore the changes in serum inflammatory cytokines,plasma homocysteine (Hcy) and hemorheology in patients with diabetic microangiopathy of different severity.Methods A total of 180 T2DM patients admitted to our hospital from January 2013 to July 2015 were selected and randomized into either the no diabetic retinopathy (NDR) group,background diabetic retinopathy (BDR) group or proliferative diabetic retinopathy (PDR) group,each including 60 patients.We compared serum hypersensitive c-reactive protein (hs-CRP),interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) levels,plasma Hcy level,and hemorheological indicators across the three groups.Results Serum hs-CRP,IL-6 and TNF-α levels,plasma Hcy level and hemorheological indicators were all significantly higher in BDR and PDR groups than in NDR group,with statistically significant differences (P ＜ 0.05).Serum hs-CRP,IL-6 and TNF-αt levels,plasma Hcy level and hemorheological indicators were all significantly higher in PDR group than in BDR group,with statistically significant differences (P ＜ 0.05).Conclusion Serum hs-CRP,IL-6 and TNF-α levels,plasma Hcy level and hemorheological indicators would raise constantly with disease progression in diabetic patients,which might be associated with the development and progression of retinopathy.

Objective To explore the impact of levocarnitine on microinflammatory state in hemodialysis patients with diabetic nephropathy(DN) .Methods Sixty outpatients and inpatients with DN in the Second Aid Hospital of Xinjiang Uygur Autonomous Region from January 2010 to December 2012 were selected as the research subjects according to the inclusion and exclusion stand‐ards and randomly divided into DN conventional treatment group (conventional treatment group ,n=30) and levocarnitine treatment observation group (levocarnitine treatment group ,n=30) .Contemporaneous 20 individuals undergoing the healthy physical exami‐nation were selected as the healthy control group ,DN was excluded .The two treatment groups were given the insulin therapy ,on the basis of the routine therapy the levocarnitine treatment group was added with levocarnitine oral solution (2 g daily) .The treat‐ment in both groups lasted for 3 weeks .The efficacy was observed and the indicators were detected before and after treatment ,in‐cluding interleukin‐6 (IL‐6) ,C‐reactive protein (CRP) ,tumor necrosis factor‐α(TNF‐α) ,urinary albumin excretion rate (UAER) , serum albumin (Alb) ,hemoglobin (Hb) ,endogenous creatinine clearance rate (Scr) ,total cholesterol (TG) and triglycerides (TC) .The above indicators in the healthy control group were detected in physical examination .Results The CRP ,IL‐6 ,TNF‐αlev‐els after treatment in the conventional treatment group and levocarnitine treatment group were higher than those in the healthy con‐trol group .The CRP ,IL‐6 and TNF‐αlevels after treatment in the levocarnitine treatment group were lower than those in the con‐ventional treatment group .The above 3 inflammatory cytokines levels after treatment in the levocarnitine treatment group were low‐er than before treatment .The TC ,TG and Ccr levels after treatment in the levocarnitine treatment group were lower than those in the conventional treatment group ,while the Alb and Hb levels were higher than those in the conventional treatment group ,the differences were statistically significant (P<0 .05) .Conclusion The microinflammation level after levocarnitine treatment in main‐tenance dialysis patients with DN is alleviated to some extent ,the renal function and nutritional status have certain improvement .

Objective To investigate the efficacy of ultrashort and short-acting insulin after subcutaneous insulin infusion pump under glucose monitoring in treatment of diabetes.Methods 110 cases with type 2 diabetes were selected,they were randomly divided into two groups with a random number table:the study group and the control group,55 cases in each group.The study group was treated with short -acting insulin after subcutaneous injection of insulin pump,the control group was treated with short -acting subcutaneous insulin infusion by insulin pump therapy, glucose monitoring system was used to monitor glucose during treatment of the two groups.The clinical efficacy after a month of treatment was observed.Results Before treatment,fasting blood glucose(FPG),postprandial 2 h blood glucose(2h PG),bedtime blood glucose of the two groups had no statistically significant differences.After treatment, FPG and 2hPG,bedtime blood glucose in the study group were (6.01 ±1.06)mmol/L,(7.90 ±0.50)mmol/L, (7.13 ±0.95)mmol/L respectively,which of the control group were (5.98 ±1.04)mmol/L,(7.91 ±0.48)mmol/L, (7.14 ±0.94)mmol/L,which were significantly lower than before treatment (t =9.843,23.669,13.569,9.683, 22.881,13.710,P <0.05),the two groups after treatment showed no significant differences(P >0.05).The blood glucose time and insulin dosage in the study group were (5.59 ±1.30)d,(39.98 ±11.01)u/d,which of the control group were (6.98 ±1.40)d,(51.89 ±11.97)u/d,and there were significant differences between the two groups(t =1.825,5.431,P <0.05).The mean blood glucose,blood glucose standard deviation,time percentage of blood glucose≥10 mmol/L,time percentage of blood glucose≤3.9 mmol/L in the study group were (5.95 ±0.61 )mmol/L, (1.28 ±0.25)mmol/L,(14.16 ±2.13)%,(6.35 ±1.45)%,which in the control group were (6.59 ±0.94)mmol/L, (1.54 ±0.29)mmol/L,(15.15 ±2.14)%,(7.96 ±1.42)% respectively,and the differences were statistically significant between the two groups(t =4.236,503.6,2.432,5.883,P <0.05).Conclusion Glucose monitoring system in monitoring blood glucose is accurate and reliable,ultra -short -acting insulin after subcutaneous insulin pump infusion in treatment of diabetes is better than short -acting insulin.

[Objective]To investigate the clinical effect of Chinese herbal medicine washout combined with lactic acid bacteria vaginal capsule on refractory vaginitis. [Method] Divide 500 patients into 3 types: downward flow of damp-heat, damp toxin implication, internal heat due of Yin deficiency, which treated with different decoctions with lactic acid bacteria vaginal capsule. Fol ow up for 3m after treatment course, observe recurrence; make vaginal secre-tion tests before and after treatment. [Result] In downward flow of damp heat type, 139 cases were cured, 11 effective, the total effective rate 100%; on damp toxin implication type, 183 cases were cured, 13 effective, total effective rate 100%;for internal heat due to Yin deficiency, 141 were cured, 9 effec-tive and total effective rate 100%. There’s no recurrence after 3m. [Conclusion] The said method can effectively regulate vaginal micro-ecological envi-ronment and reduce recurrence of refractory vaginitis, with safe clinical application and definite cure effect.

[Objective]To investigate the characteristics of the distally based sural neurofasciocutaneous flap for repairing soft tissue defects in heel.[Method]From June 2002 to July 2008,58 cases of soft tissae defects in heal were repaired with distally based sural neurofasciocutaneous flaps.Among them,10 cases combined with a dead cavity,13 cases were innervated with the anastomosis of the sural nerve to the cutaneous nerve of recipient site.The size of the flaps ranged from 8 cm?4 cm to 20 cm?15 cm.The perforating barnches of peroneal artery in fascia pedicle were exposed first,and then the flaps were harvested.[Result]Forty-six flaps survived uneventfully.Necrosis took place in the distal part in 12 flaps,and the wounds healed after changing dressing daily,two-stage suture or skingrafting.Through 1 to 34 months of follow-up,all the survival flaps provided good contours.Infection was controlled and no recurrence,ulcer or pressure sore was found.Walk function was good.[Conclusion]Distally based sural neurofasciocutaneous flaps for reparing soft tissue defects have many advantages.It can offer abundant blood supply,large survival size,simple operation,high achievement rate,and re-establishment of protective sensation.The flaps can repair almost all defects in heel.

Objective To detect the expression of humar telomerase reverse transcriptase (hTERT) and telomerase activity in HeLa,MCF-7,SMMC7721 PC-3m and U2OS cell lines. Methods The techniques of immunochemistry and TRAP-ELISA were employed to detect the expression of hTERT and telomerase activity in different tumor cell lines.Results The positive rate of hTERT in HeLa cells(93.75%?3.10%)was significantly higher than that in U2OS cells(2.75%?0.96%),besides the other three cell lines showed an positive rates of hTERT in MCF-7(92.50%?2.65%),SMMC7721 (53.75%?2.22%)and PC-3m(23.50%?2.89%); Meanwhile,the telomerase activity of HeLa cells (94.58%?3.49%) was also much higher than that of U2OS(3.02%?0.43%),likewise the telomerase activities of the other three cell lines (MCF-7,SMMC7721,PC-3m) were 73.90%?4.50%,66.67%?3.35% and 50.62%?1.96%, respectively. Conclusion The expression of hTERT and telomerase activity show obvious differences among five tumor cell lines,suggesting that telomerase inhibitors cannot effect on all the tumor cell lines.

Objective To explore the method of obtaining and expanding epithelial stem cells(ESCs) from plucked hair follicle in vitro and clarify that fibroblasts are necessary for ESCs growth.Methods A amount of ESCs from plucked hair were obtained,the epithelial outgrowth of human plucked hair follicle was studied and compared between different culture conditions including no coating well,rat tail-collagen coating well,collagen Ⅳ coating well and 3T3 fibroblasts feeder.The characteristics of these cells were detected by RT-PCR and immunofluorescent staining for CK15.Results After compared between different culture conditions including no coating well,rat tail-collagen coating well,collagen Ⅳ coating well and 3T3 fibroblasts feeder,the results indicated that the plucked hair fragment B3(bulge area of hair follicle) placing on 3T3 fibroblasts feeder was the optimum condition.RT-PCR and immunofluorescent method confirmed that these cells expressed CK15 which was the special marker of ESCs.Conclusion ESCs can be obtained from bulge area of hair follicle which grows on the 3T3 fibroblasts feeder and this method is the optimum condition for ESCs growth after compared between different conditions.

Objective To investigate the effects of docosahexaenoic acid (DHA) on action potential (AP)and transient outward potassium channel (Ito) in rat ventricular myocytes in order to evaluate the anti-arrhythmia mechanism of DHA. Method The rat ventricular myocytes were isolated by using enzyme digestion method. AP and Ito of individual ventricular myocyte were recorded by using patch-clamp technique in whole-cell configuration at room temperature. The effects of DHA on AP and Ito were observed when it was applied in 0 μmol/L, 20 μmol/L, 40 μmol/L, 60 μmol/L, 80 μmol/L, 100 μmol/L and 120μmol/L separaterly. Results The 25%,50% and 90% of action potential duration (APD25, APD50 and APD90) were gradually prolonged with the escalation of concentration of DHA ( P ＜ 0.05, n= 20). The effects of DHA of different concentrations on AP maximal velocity (Vmax), AP amplitude (APA) and AP overshoot (OS) did not produce significantly different results (P ＞ 0.05, n= 20). The degree of blockade of Ito was concentration-dependent as different concentrations of DHA were applied, and as the concentration of DHA was escalated, the I-V curves went downwards, the stably inactivated curves shifted to the lift, and the time taken for recovery from inactivation prolonged ( P ＜ 0.05, n =20). However, the different concentrations of DHA did not produce different effects on stably activated curves ( P＞ 0.05). The Itos were blocked to (2.61 ± 0.26)%, (21.79±4.85)%, (63.11 ± 6.57)%, 75.52±7.26 ) %, (81.82 ± 7.63) % and (84.33 ± 8.25) % by the above given concentrations of DHA respectively under given potential equaling to + 70 mV( P ＜ 0.05, n = 20), and the half-effect concentration (EC50) of DHA on Ito was(49.11±2.68) umol/L. Conclusions The effects of DHA on APD and Ito may be one of the anti-arrhythmia mechanism of DHA.

Background and purpose:As a member of the catenin family, Delta-catenin protein could promote proliferation and invasion of tumor cells, but the accurate mechanism of Delta-catenin promoting cell proliferation is not clear. In the present study, we illustrated that Delta-catenin’s effect on cell apoptosis and their relationship with mitogen-activated protein kinase (MAPK) signaling pathway, and the possible mechanism was also explored for Delta-catenin promoting invasion and proliferation of tumor cells. Methods:The alterations of p38 and c-jun N-terminal rinasel JNK protein activity were detected in SPC and SK lung cancer cell lines with Delta-catenin overexpression or not, by Western blot method. At the same time, the apoptotic number of tumor cells was also examined by FCM method. Furthermore, the number of invasive tumor cells was examined by Matrigel invasive experiment. Results:Compared with untreated group and empty vector group, the activity of p38 protein was unchanged in lung cancer cell lines with Delta-catenin overexpressed (P>0.05), but the activity of JNK protein was decreased signiifcantly (P<0.05),meanwhile, apoptotic proportion of tumor cells were also reduced (P<0.05), and invasive ability of tumor cells was enhanced signiifcantly (P<0.05). Conclusion:Delta-catenin probably decreases apoptosis number of lung cancer cells via inhibiting the activity of JNK pathway, and then promotes invasive ability of tumor cells.

Objective To investigate the effect of point Zusanli acupuncture on serum creatine kinase (CK) and creatine kinase isoenzyme (CK-MB) in high-intensity military training soldiers and explore its possible antifatigue mechanism.Methods Sixty healthy soldiers were randomly allocated to groups A and B, 30 each. Group A received acupuncture at point Zusanli and group B, no treatment. After two weeks of treatment, two groups of soldiers separately did 5 km high-intensity running training and fulfilled the requirement of army examination. CK and CK-MB were measured in two groups before and after training and five days after training.Results In the two groups, there were statistically significant differences in serum CK and CK-MB contents between before training and after or five days after training (P<0.05). After or five days after training, there were statistically significant differences in serum CK and CK-MB contents between groups A and B (P<0.05).ConclusionsAcupuncture at point Zusanli can effectively improve bodily antifatigue ability and reduce myocardial and skeletal muscle injury caused by high-intensity exercise in military training soldiers.