1.Value of modified Ziehl-Neelsen acid-fast staining of bronchoalveolar lavage fluid in diagnosis of pulmonary tuberculosis with negative sputum
Jiping ZHANG ; Xiaojin LIU ; Yingbin YE ; Xiuxiang HUANG ; Liheng ZHENG
International Journal of Laboratory Medicine 2014;(13):1702-1703
Objective To establish the modified Ziehl-Neelsen acid -fast staining method and to investigate the value of modi-fied Ziehl-Neelsen acid-fast staining method of bronchoalveolar lavage fluid in the diagnosis of pulmonary tuberculosis(TB)with negative sputum specimen.Methods 50 cases of negative sputum pulmonary TB were performed the bronchoalveolar lavage by the fiberoptic bronchoscope before the treatment,at the same time,the bronchoalveolar lavage fluids were collected and detected by the two methods of the traditional and modified Ziehl-Neelsen staining.The diagnostic positive rates were compared between the two groups.Results The positive rates of the two kinds of acid-fast staining method were 38% and 82% respectively,the difference showing statistical significance(P <0.05 ).Conclusion The modified Ziehl-Neelsen acid-fast staining of bronchoalveolar lavage fluid can highly improve the positive diagnostic rate of pulmonary TB patients with negative sputum and deserves to be clinically promoted.
2.miR-410 expression and bioinformatics analysis of its predicted target genes in systemic lupus erythematosus
Ji ZHANG ; Daming OU ; Lifang HUANG ; Yanglin OU ; Liheng ZOU
Chinese Journal of Immunology 2017;33(6):884-888
Objective:To detect expression level of miR-410 in patients with systemic lupus erythematosus (SLE),and to expose the role of miR-410 and its target genes by bioinformatics methods.Methods:Expression level of miR-410 were detected by quantitative RT-PCR in peripheral blood mononuclear cells of SLE patients,and miR-410 sequence,its target genes and Genecards database were analyzed,and analysis of GO enrichment and KEGG Pathway was further performed.Results:miR-410 expression was significantly reduced in SLE patients,and its nucleotide sequence was highly conserved among species.These genes that were predicted to be regulated by miR-410 and associated with LE pathogenesis,included FASLG,CSF2,IFNAR2,MAPK1,PLCG2,IL4 and other genes.Analysis of GO enrichment revealed that miR-410's target genes were involved in cell growth,proliferation,programmed cell death,cell differentiation,immune system development and other biological activities.Analysis of KEGG Pathway showed that the target genes of miR-410 were significantly enriched in a series of signaling pathways including pathways in cancer,cytokine-cytokine receptor interaction,glioma,melanoma,TGF-β and JAK-STAT signaling pathway.Conclusion:miR-410 maybe directly regulate its target molecules,mediate various signal pathway networks,thus participate in the occurrence and development of SLE.
3.Comparative study of USPIO-enhanced and Gd-enhanced MRI in the diagnosis of atherosclerotic plaques in rabbits
Luchao WANG ; Gongxin LI ; Peng LIU ; Zhibo WEN ; Fanheng HUANG ; Liheng CHEN ; Xin ZHAO ; Lin LIN ; Yijun ZHOU
The Journal of Practical Medicine 2014;(5):684-687
Objective To investigate the imaging characteristics of USPIO-enhanced and Gd-enhanced MRI in atherosclerosis and to compare the detection rate of atherosclerotic plaque between them. Methods Thirty-five healthy male rabbits were assigned to experimental group (n=30) to establish a model of atherosclerosis by damaging aortic tunica intima with Foley′s tube in combination with a high fat diet and 5 to control group without any intervention. At week 12, USPIO-enhanced and Gd-enhanced MRI scanning were conducted to compare the signal changes of atherosclerotic plaque before and after enhancement with the 2 contrast media. Ninety seven pictures were randomly selected respectively from the pictures enhanced by the 2 contrast media to compare the detection rate plaque between them. Pthology examination was used for detection standard. For the control group , pictures were randomly selected. Results In the experimental group, 7 rabbits died of Foley′s tube damaging, 2 died of raising and 1 died of anesthesia. All 5 rabbits in control group survived. A total of 172 pathological sections were made with 134 plaques and 72 vulnerable plaques pathologically confirmed. In pictures enhanced by USPIO , 84 plaques were confirmed by HE staining with a detection rate of 86.6%. In pictures enhanced by Gd, 72 plaques were confirmed by HE staining with a detection rate of 74.2%. Detection rate of USPIO-enhanced MRI in atherosclerosis plaque was significantly higher than that of Gd-enhanced MRI (X2=3.96, P=0.046). Conclusion USPIO shows its superiority as a new contrast medium in detection of atherosclerosis plaque.
4.Animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells/chondrocytes for repair of composite osteochondral defects in rabbit knee joints
Wencheng TAN ; Zhengang ZHA ; Jiaqing ZHANG ; Liheng ZHENG ; Yaozhong LIANG ; Jisheng XIA ; Xinpei HUANG ; Hao WU ; Hongsheng LIN
Chinese Journal of Tissue Engineering Research 2011;15(12):2265-2269
BACKGROUND: Though there were many experiments addressing repairing osteochondral defects before, faulty restoration occurred at coupling interfaces. OBJECTIVE: To investigate the feasibility of repairing of osteochondral composite defects in rabbit knees with animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells (BMSCs)/chondrocytes.METHODS: New Zealand white rabbits were randomly divided into the experimental, control and blank groups and prepared for unilateral knee joint osteochondral defects. Animal-origin osteochondral scaffold combined with BMSCs/chondrocytes, animal-origin osteochondral scaffold and no material was implanted to repair the defects in the experimental, control and blank groups, respectively. Healing condition was evaluated by gross observation, hematoxylin-eosin staining, and toluidine blue staining at 4, 8, and 12 weeks after operation. RESULTS AND CONCLUSION: At 12 weeks after operation, gross observation showed the defects were repaired completely without local depression and the regenerated tissues were fused with surrounding tissues in the experimental group. Hematoxylin-eosin staining and toluidine blue staining revealed that there were many new hyaline cartilages in the cartilage defects in which columnar cells were lined well and cartilage lacuna was obviously, also, there were many bony tissues in the bone defects. The regeneration cartilage, the underlying subchondral bone and host bone were coupled completely. The toluidine blue positive rate and histologic scores of the experimental group were superior to those of the control and blank groups (P < 0.05). It is demonstrated that animal-origin osteochondral scaffold combined with BMSCs/chondrocytes is an ideal method to repair defects between cartilage and the underlying subchondral bone.
5.One case of Mycoplasma hominis-related intracranial infection after craniocerebral injury
Lijuan CHEN ; Liheng ZHENG ; Jing LIN ; Xue HUANG
Chinese Journal of Laboratory Medicine 2022;45(2):197-199
The patient, a 50-year-old male, was admitted to the hospital on April 1, 2020 with the chief complaint of "confusion with vomiting for 1 hour due to falling from height ", and the emergency craniotomy was performed. Intermittent fever with a maximum temperature of 38 ℃ occurred 3 days after the surgery, and the inflammation indexes were all higher than the upper limit of the reference values. Recurrent fever remained despite after empirical anti-infection treatment. On April 12, the patient was treated with vancomycin combined with meropenem after cerebrospinal fluid specimens routine and biochemical tests suggested intracranial infection. After 48 hours of cultivating the cerebrospinal fluid and blood specimens, some small, clear, needle-like colonies were found and they were identified as Mycoplasma humanum by using 16S rRNA gene. Eventually, the patient died due to the severity of the disease and complications.
6.Ultrasmall superparamagnetic iron oxide-enhanced magnetic resonance angiography combined with inflammatory factors for detecting atherosclerotic plaques in rabbits.
Gongxin LI ; Luzhao WANG ; Peng LIU ; Zhibo WEN ; Fanheng HUANG ; Liheng CHEN ; Xin ZHAO ; Lin LIN ; Yijun ZHOU
Journal of Southern Medical University 2014;34(9):1324-1328
OBJECTIVETo investigate the feasibility of Ultrasmall superparamagnetic iron oxide-enhanced magnetic resonance angiography (USPIO-MRA) combined with interleukin-6 (IL-6) and IL-10 detection for detecting atherosclerotic plaques in rabbits.
METHODSTwenty-four normal male rabbits were randomly assigned (n=8) into group A with atherosclerosis induced by damaging the aortic tunica intima with Foley's tube in combination with a high fat diet, group B with a high fat diet, and group C without any intervention. At week 12, plain and USPIO-MRA was performed in all the 24 rabbits and the results were compared with pathological examinations; blood samples were collected from the ear vein to examine blood lipids and levels of IL-6 and IL-10.
RESULTSThe rabbits in groups A and B showed significantly different IL-6 levels (167 ± 21.3 vs 116 ± 14.3 pg/ml, P<0.05) but comparable blood lipids and IL-10 levels (P>0.05). The levels of IL-6, IL-10, TC, TG, and LDL, but not HDL, differed significantly between groups A and C and between groups B and C (P<0.01). Continuous MRA scan showed significantly different signal-to-noise ratios (SNR) between the 3 groups.
CONCLUSIONUSPIO-MRA combined with IL-6 and IL-10 detection is feasible in detecting atherosclerotic plaques in rabbits.
Animals ; Atherosclerosis ; Contrast Media ; Dextrans ; Interleukin-10 ; analysis ; Interleukin-6 ; analysis ; Magnetic Resonance Angiography ; Magnetite Nanoparticles ; Male ; Plaque, Atherosclerotic ; diagnosis ; Rabbits
7.Patients with very late-onset neuromyelitis optica spectrum disorder:clinical analysis of 12 cases
Hui LI ; Xiaoxi HUANG ; Liheng LI
Journal of Apoplexy and Nervous Diseases 2020;37(3):213-218
Objective Report and analyze the clinical features,diagnosis,and prognosis of 3 patients with elderly-onset(age>75 years) neuromyelitis Optica spectrum disorders(NMOSDs),reviewed previous pieces of literature to improve the risk and understanding of NMOSD in elderly patients. Methods We searched the domestic and international databases in the past 10 years,to collect the case report of patients with NMOSDs over the age of 75 years,along with three cases discovered in the neurology department of the First Affiliated Hospital of Zhengzhou University. The clinical features,laboratory tests and treatment,were retrospectively analyzed. Results A total of 12 patients with NMOSDs>75 years old were enrolled in this study,including 7 females and 5 males,with an average age of onset(82.67±4.42) years. All patients were diagnosed with longitudinally extensive transverse myelitis(LETM),and the single-phase course was the majority. The AQP4 test was positive in all cases and the MRI was illustrated that mainly caused the thoracic spinal cord. Twelve cases were treated with intravenous methylprednisolone(IVMP)1 g daily for 3 to 5 days,3 cases were combined with plasma exchange(PLEX),4 cases were treated with immunosuppression,and 5 cases had a relapse,9 cases were effective,2 cases were markedly effective,3 cases were worsened. Three patients died during treatment and follow-up. Conclusion Patients with very late-onset NMOSDs are more likely to have LETM,higher disability rates. IVMP plus PLEX is more effective than IVMP alone,But the benefit of immunosuppression is unclear in clinical practice and observational studies.
8.Effects of threshold inspiratory muscle training on respiratory function, motor function and quality of life for patients with chronic obstructive pulmonary disease: a meta-analysis
Juan PENG ; Jieping WANG ; Wei HUANG ; Bishuang FAN ; Jihua YU ; Jin ZENG ; Liheng HUANG ; Lijuan AN ; Fangyuan XU
Chinese Journal of Rehabilitation Theory and Practice 2022;28(9):1022-1031
ObjectiveTo systematically evaluate the effects of threshold inspiratory muscle training (TIMT) on respiratory function, motor function and quality of life in patients with chronic obstructive pulmonary disease (COPD). MethodsRandomized controlled trials (RCT) about the effects of TIMT on dyspnea, quality of life, motor function and inspiratory muscle strength for COPD patients were retrieved from PubMed, EBSCO, Web of Science, Ovid, Cochrane Library, SinoMed, CNKI, VIP, since establishment to September, 2020. Two researchers independently screened literatures, extracted data and evaluated the methodological quality. A meta-analysis was performed using RevMan 5.3. ResultsA total of 30 RCTs involving 2 060 patients were included. TIMT could obviously improve the maximum inspiratory pressure (MD = 10.68, 95%CI 7.43 to 13.92, P < 0.001), optimize the results of 6-minute Walking Test (MD = 24.62, 95%CI 9.09 to 40.15, P = 0.002), the St George's Respiratory Questionnaire (MD = -3.08, 95%CI -5.84 to -0.33, P = 0.03), the modified Medical Research Council Dyspnea Scale (MD = -0.30, 95%CI -0.52 to -0.07, P = 0.01) and Borg score (MD = -0.84, 95%CI -1.24 to -0.44, P < 0.001). TIMT could also improve the forced expiratory volume in one second (MD = 0.11, 95%CI 0.04 to 0.19, P = 0.003) and the forced expiratory volume in one second in predicted (MD = 3.72, 95%CI 2.62 to 4.82, P < 0.001). There was no significant difference in the COPD Assessment Test (MD = -1.14, 95%CI -2.32 to 0.03, P = 0.06) or forced vital capacity (MD = 0.07, 95%CI -0.12 to 0.25, P = 0.49). ConclusionTIMT can improve the inspiratory muscle strength, alleviate the symptoms of dyspnea, and improve the lung function and the quality of life for COPD patients.