1.Chemical constituents from fruits of Ligustrum lucidum.
Xiaojun HUANG ; Zhiqi YIN ; Wencai YE ; Wenbin SHEN
China Journal of Chinese Materia Medica 2010;35(7):861-864
OBJECTIVETo study the chemical constituents from the fruits of Ligustrum lucidum.
METHODThe chemical constituents from the ethanol extract of L. lucidum were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatographic methods. Their structures were identified on the basis of spectroscopic data and physico-chemical properties.
RESULTTwenty compounds were isolated and identified as oleanolic acid (1), crategolic acid (2), acetyl oleanolic acid (3), lupeol (4), betulin (5), dammarenediol-II (6), 3beta-acetyl-20, 25-epoxydammarane-24alpha-ol (7), 25-epoxydammarane-3beta, 24alpha-diol (8), dammar-24-ene-3beta-acetyl-20S-ol) (9), 20S, 24R-dammarane-25-ene-24-hydroperoxy-3beta, 20-diol (10), fouquierol (11), oliganthas A (12), dammarenediol II 3-O-palmitate (13), ocotillol II 3-O-palmitate (14), (E) -25-hydroperoxydammar-23-ene-3beta,20-diol (15), verbascoside (16), cimidahurinine (17), 2-(3,4-dihydroxyphenyl)-ethyl-O-beta-D-glucopyranoside (18), osmanthuside H (19), 2-(3,4-dihydroxyphenyl) ethanol (20).
CONCLUSIONCompounds 4, 16,17, 19 were isolated from this plant for the first time, andcompounds 12-15 were isolated from this genus for the first time.
Fruit ; chemistry ; Ligustrum ; chemistry ; Organic Chemicals ; analysis ; isolation & purification
2.Study on quality characteristics of Ligustri Lucidi Fructus in national resource survey and consideration on standards of Ligustri Lucidi Fructus in Chinese Pharmacopoeia.
Huan LIU ; Hui XIONG ; Xue XUE ; Meng-Nan LIU ; Hai-Li WANG ; Wei LIU ; Feng WEI ; Xiang-Ri LI
China Journal of Chinese Materia Medica 2019;44(1):68-76
The content of tyrosol,salidroside,echinacoside,rutin,acteoside,ligustroflavone,specnuezhenide,and quercetin were determined by HPLC,and the color of Ligustri Lucidi Fructus was determined by comparison with color card.Hundred-seed weight was analyzed by using gravimetric method.The correlation analysis and One-way ANOVA were used to analyze the relationship between the characters,the chemical composition,the harvest time and the geographical location of Ligustri Lucidi Fructus,for giving a comprehensive evaluation of the quality of Ligustri Lucidi Fructus The results showed that 92% of Ligustri Lucidi Fructus were all up to quality standard of Chinese Pharmacopoeia,and the contents of 7 components in Ligustri Lucidi Fructus(except quercetin) were higher than those in samples with black colors.The content of salidroside in Ligustri Lucidi Fructus harvested in June was the highest and the other7 components of Ligustri Lucidi Fructus were relatively high in 8-10 months.According to the quality parameters of Ligustri Lucidi Fructus,the Ligustri Lucidi Fructus from six habitats can not be distinguished effectively.The results showed that there was a certain relationship between the color,harvest season and component content of Ligustri Lucidi Fructus,and the habitats were not related to the quality parameters of Ligustri Lucidi Fructus.The study aimsat providing data support for the resource status of native Ligustri Lucidi Fructus,and a theoretical basis for the revision of standards of Ligustri Lucidi Fructusin the future.
Drugs, Chinese Herbal
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standards
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Fruit
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chemistry
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Ligustrum
;
chemistry
3.Content change of main components in processed Ligustrum lucidum fruits of different processing time and their characteristic quality standard based on UPLC technology.
Xin JI ; Xiao-Qian LIU ; Chun LI ; Li-Xin YANG ; Wei-Hong FENG ; Zhi-Min WANG
China Journal of Chinese Materia Medica 2018;43(24):4862-4868
To investigate the dynamic change law of the main components in Ligustri Lucidi Fructus during the wine-steaming process and attempt to establish the characteristic quality standard of wine-steamed Ligustri Lucidi Fructus by determining the content of salidroside and specnuezhenide using Ultra Performance Liquid Chromatography (UPLC) technology at different processing time points (12, 15, 18, 21, 24 h). The chromatographic separation was performed on Waters Acquity UPLC®BEH C₁₈ column (2.1 mm×50 mm, 1.7 μm) with acetonitrile and 0.2% formic acid aqueous solution as the mobile phase for gradient elution; and the detection wavelength was set at 280 nm; the flow rate was 0.5 mL·min⁻¹, and the column temperature was set at 40 °C. The results showed that the two components were well separated in the above conditions. The salidroside and specnuezhenide showed a good linear relationship within the range of 10.19-326 ng and 49.53-1 585 ng, respectively. Their average recovery was 103.4% and 101.7% with RSD of 0.81% and 0.79%, respectively. With the extension of processing time, the content of specnuezhenide was decreased, while salidroside was gradually increased. For the 27 batches of Ligustri Lucidi Fructus, the content of salidroside was between 0.042 5% and 0.192 4%, and that of specnuezhenide was between 0.829 7% and 5.218 0%. While for the 25 batches of wine-steamed Ligustri Lucidi Fructus, the content of the first one was between 0.229 2% and 1.045 8%, and the latter one was between 0.743 8% and 3.645 4%. As compared with Ligustri Lucidi Fructus, the ratio of specnuezhenide to salidroside was significantly decreased in the wine-steamed Ligustri Lucidi Fructus. According to the experimental results, the quality standard of Ligustri Lucidi Fructus is tentatively fixed as follows: the content of specnuezhenide shall not be less than 0.80%, and the ratio of specnuezhenide content/salidroside content (Sp/Sa) should not be smaller than 15. As for the wine-steamed ones, the content of salidroside should not be less than 0.20%, and specnuezhenide content should not be less than 0.70%; Sp/Sa should not be greater than 8. The method established in this study is simple and reliable, which could be used for the content detection of salidroside and specnuezhenide in Ligustri Lucidi Fructus samples. The characteristic quality standard established in this study could be used to distinguish the Ligustri Lucidi Fructus and wine-steamed Ligustri Lucidi Fructus.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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Fruit
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Ligustrum
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Wine
4.Complete chloroplast genome of Ligustrum lucidum and highly variable marker identification for Ligustrum.
Yuan-Xi JIN ; Yu-Shuang WANG ; Yong-Wei GAO ; Li-Wei ZHOU ; Yi-Heng WANG ; Qing-Jun YUAN ; Wen-Pan DONG
China Journal of Chinese Materia Medica 2022;47(7):1847-1856
Ligustri Lucidi Fructus, the sun-dried mature fruit of Ligustrum lucidum, is cool, plain, sweet, and bitter, which can be used as both food and medicine, with the effects of improving vision, blacking hair, and tonifying liver and kidney. It takes effect slowly. However, little is known about the genetic information of the medicinal plant and it is still a challenge to distinguish Ligustrum species. In this study, the complete chloroplast genome of L. lucidum was obtained by genome skimming and then compared with that of five other Ligustrum species, which had been reported. This study aims to evaluate the interspecific variation of chloroplast genome within the genus and develop molecular markers for species identification of the genus. The result showed that the chloroplast genome of L. lucidum was 162 162 bp with a circular quadripartite structure of two single-copy regions separated by a pair of inverted repeats. The Ligustrum chloroplast genomes were conserved with small interspecific difference. Comparative analysis of six Ligustrum chloroplast genomes revealed three variable regions(rbcL-accD, ycf1a, and ycf1b), and ycf1a and ycf1b can be used as the species-specific DNA barcode for Ligustrum. Phylogeny analysis provided the best resolution of Ligustrum and supported that L. lucidum was sister to L. gracile. This study clarified the genetic diversity of L. lucidum from provenance, which can serve as a reference for further analysis of pharmacological differences and breeding of excellent varieties with stable drug effects.
Fruit
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Genome, Chloroplast
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Ligustrum/genetics*
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Phylogeny
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Plant Breeding
5.Species identification of Ligustrum lucidum.
Yu-Shuang WANG ; Yuan-Xi JIN ; Kang-Jia LIU ; Chang GUO ; Yi-Heng WANG ; Chao XU ; Zhi-Xiang ZHANG ; Wen-Pan DONG
China Journal of Chinese Materia Medica 2023;48(11):2940-2948
Ligustrum lucidum is a woody perennial plant of genus Ligustrum in family Oleaceae. Its dried fruit has high medicinal value. In this study, the authors evaluated the variability and species identification efficiency of three specific DAN barcodes(rbcL-accD, ycf1a, ycf1b) and four general DAN barcodes(matK, rbcL, trnH-psbA, ITS2) for a rapid and accurate molecular identification of Ligustrum species. The results revealed that matK, rbcL, trnH-psbA, ITS2 and ycf1a were inefficient for identifying the Ligustrum species, and a large number of insertions and deletions were observed in rbcL-accD sequence, which was thus unsuitable for development as specific barcode. The ycf1b-2 barcode had DNA barcoding gap and high success rate of PCR amplification and DNA sequencing, which was the most suitable DNA barcode for L. lucidum identification and achieved an accurate result. In addition, to optimize the DNA extraction experiment, the authors extracted and analyzed the DNA of the exocarp, mesocarp, endocarp and seed of L. lucidum fruit. It was found that seed was the most effective part for DNA extraction, where DNAs of high concentration and quality were obtained, meeting the needs of species identification. In this study, the experimental method for DNA extraction of L. lucidum was optimized, and the seed was determined as the optimal part for DNA extraction and ycf1b-2 was the specific DNA barcode for L. lucidum identification. This study laid a foundation for the market regulation of L. lucidum.
Ligustrum/genetics*
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Seeds
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Fruit
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Polymerase Chain Reaction
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Research Design
6.Identification of chemical compositions in ether extracts of Ligustrum lucidum by GC-MS and chemometrics.
Xiangli WANG ; Liangxiao ZHANG ; Yizeng LIANG ; Hui DAI
China Journal of Chinese Materia Medica 2010;35(17):2303-2307
OBJECTIVETo identify and quantify the compound compositions of ether extracts of Ligustrum lucidum.
METHODAfter extracted 4 hour by Soxhlet extraction with ether, the extractive were analyzed by gas chromatography and mass spectrometry (GC-MS). Furthermore, heuristic evolving latent projection (HELP) was used to resolve the overlapping peaks to obtain the pure concentration profiles and mass spectra. Subsequently, mass spectral similarity search combining with the retention index was employed for the identification of each component.
RESULTTotally, 75 compounds were identified, accounting for 84.3% of that in the whole ether extracts of L. lucidum.
CONCLUSIONThe main compounds of L. lucidum are oleic acid, lupeol and (Z,Z) 9,12-octadecadienoic acid, whose relative content were 9.805%, 8.848%, 8.357%, respectively.
Ether ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Ligustrum ; chemistry ; Plant Extracts ; analysis ; isolation & purification
7.Quantitative analysis of two major secoiridoid glucosides in fruits of Ligustrum lucidum.
China Journal of Chinese Materia Medica 2008;33(18):2099-2102
OBJECTIVETo develop an HPLC method for determination of two major secoiridoid glucosides (nuezhenoside and G13) from Fructus Ligustri Lucidi. and determinae nuezhenoside and G13 in samples from different acquisition times and different places.
METHODThe sample was separated on a YMG-C18 (4.6 mm x 250 mm, 10 microm) column. The mobile phase was acetonitrile-water, gradient elution (CH3CN: 0 min, 20%; 15 min, 25%) with the flow rate of 1 mL x min(-1), the detection wavelength was 240 nm, and the column temperature was set at 25 degrees C.
RESULTIn HPLC analysis ,the linear ranges of nuezhenoside and G13 were 0.341-34.1 microg and 0.331-33.1 microg, respectively; their average recoveries were 97.5% (RSD 1.35%) and 98.1% (RSD 1.82%), respectively. The results of content determination (nuezhenoside and G13) of samples from different places varied greatly. This may be caused by different species sources and different preparation methods, etc. The experiment led up to the fact that from the beginning to the middle of November the content of nuezhenoside and G13 reached the maximum.
CONCLUSIONThe established method can be used to determine two major secoiridoid glucosides (nuezhenoside and G13) in Fructus Ligustri Lucidi simultaneously. Meanwhile it can be used for the quality control of Fructus Ligustri Lucidi.
Chromatography, High Pressure Liquid ; Fruit ; chemistry ; Iridoids ; chemistry ; Ligustrum ; chemistry ; Reproducibility of Results
8.Comparative study on HPLC fingerprints between crude and processed Ligustri Lucidi Fructus.
Hao-Jie ZHANG ; Huan-Ru LI ; Zhi-Yi FENG ; Ke LI ; Yan-Ping HU ; Su-Xiang FENG
China Journal of Chinese Materia Medica 2020;45(16):3871-3876
To establish high performance liquid chromatography(HPLC) fingerprints for crude and processed Ligustri Lucidi Fructus,and to evaluate their quality through the similarity calculation and chemical pattern recognition. The separation was performed with Syncronis C_(18) column(4.6 mm × 250 mm, 5 μm), with acetonitrile(A) and 0.1% phosphoric acid solution(B) as the mobile phase for gradient elution, and a detection wavelength of 280 nm. HPLC was used to detect 22 batches of crude and processed Ligustri Lucidi Fructus,and the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition) was used to evaluate the similarity among 22 batches. The research on pattern recognition was conducted with cluster analysis(CA), principal component analysis(PCA), and partial least squares discriminate analysis(PLS-DA). HPLC fingerprints of crude and processed Ligustri Lucidi Fructus were established, with similarity ranging from 0.9 to 1.0. The crude and processed Ligustri Lucidi Fructus can be obviously distinguished by using CA, PCA and PLS-DA. According to the results of PLS-DA,11 constituents including hydroxytyrosol, tyrosol, specnuezhenide and oleuropein were the main marker components leading to the difference. The established fingerprint method is stable and reliable, and can provide method basis for quality control of crude and processed Ligustri Lucidi Fructus. Chemical pattern recognition is proved to be helpful in comprehensive quality control and evaluation of Ligustri Lucidi Fructus before and after the process.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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Fruit
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Ligustrum
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Medicine, Chinese Traditional
9.Qualitative and quantitative analysis on triterpenoids in Ligustri Lucidi Fructus.
Xin JI ; Xiao-Qian LIU ; Lu GAO ; Su-Ping XIAO ; Yao-Hua LIANG ; Chun LI ; Zhi-Min WANG
China Journal of Chinese Materia Medica 2021;46(5):1168-1178
Triterpenoids are one of the most active constituents in Ligustri Lucidi Fructus, but only oleanolic acid has been mostly studied. In recent years, a growing number of studies have shown that other triterpenes from Ligustri Lucidi Fructus also have various biological activities, so it is necessary to build up a detailed profile of the triterpenoids in Ligustri Lucidi Fructus. The chromatographic separation was performed on a C_(18) column(4.6 mm×250 mm, 5 μm) with mobile phase of methanol-acetonitrile-0.2% formic acid for gradient elution. The detection wavelength was set at 210 nm, with a flow rate of 0.5 mL·min~(-1), and the column temperature of 25 ℃. The HPLC fingerprint of triterpenoids in Ligustri Lucidi Fructus was built by testing 21 batches of samples from different sources. The structures of the total 15 common chromatographic peaks were elucidated with UHPLC-ESI-Orbitrap-MS/MS technique and six of them were identified as tormentic acid, pomolic acid, maslinic acid, botulin, oleanolic acid and ursolic acid by comparison to the reference substances. Under the same chromatographic condition, four main triterpenes(podocarboxylic acid, hawthorn acid, oleanolic acid and ursolic acid) were quantified and the results of system adaptability and methodology investigation all met the requirements of content determination. Meanwhile, with oleanolic acid(A) as the internal reference substance, quantitative analysis of multi-components by single marker(QAMS) method was used to analyze the above four components. The relative correction factor of oleanolic acid(B), hawthorn acid(C) and ursolic acid(D) to oleanolic acid was f_(B/A)=1.12, f_(C/A)=1.02 and f_(D/A)=0.88, respectively, and the relative retention values of these three to oleanolic acid was RRV_(B/A)=0.46, RRV_(C/A)=0.70 and RRV_(D/A)=1.03, respectively. The contents determined by two methods were similar. In conclusion, the method built in this paper is proved to be simple, reliable and specific for the simultaneous qualitative and quantitative analysis of the triterpenoids in Ligustri Lucidi Fructus, which can lay foundation for further assays of the triterpenoids in Ligustri Lucidi Fructus and the relative products.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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Fruit
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Ligustrum
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Tandem Mass Spectrometry
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Triterpenes
10.Mechanism and experimental verification of "Polygoni Cuspidati Rhizoma et Radix-Ligustri Lucidi Fructus" combination in treatment of acute gouty arthritis based on network pharmacology.
Kang DU ; Wei-Ding WANG ; Bin HAN ; Yi-Fei WANG ; Zhi-Ping WANG
China Journal of Chinese Materia Medica 2022;47(6):1677-1686
Based on network pharmacology, the mechanism of Polygoni Cuspidati Rhizoma et Radix-Ligustri Lucidi Fructus(PL) combination against acute gouty arthritis(AGA) was explored and preliminarily verified by animal experiment. The chemical components and corresponding targets of PL were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). The active components with oral bioavailability(OB)≥30% and drug-likeness(DL)≥0.18 were screened based on literature, and the related protein targets were collected. Then the protein targets were standardized with the help of UniProt database. The AGA-related targets were searched from GeneCards, NCBI, and DrugBank. The common targets of the disease and the medicinals were yielded by FunRich V3, and the protein-protein interaction(PPI) network was constructed to screen the key targets, followed by Gene Ontology(GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis of the key targets. Afterwards, some of the key targets were verified by sodium urate crystal-induced AGA mouse model. A total of 25 active components and 287 targets of PL, 811 targets of AGA, and 88 common targets were screened out. PPI network analysis showed that tumor necrosis factor(TNF), interleukin-6(IL-6), and interleukin-1β(IL-1β) may be the core targets of PL in the treatment of AGA. The key targets were mainly involved in 566 GO terms(P<0.05), including multiple biological processes such as inflammatory response and immune response. Moreover, they were related to 116 KEGG pathways and these pathways were involved in inflammation and immunity, mainly including NOD-like receptor signaling pathway and TNF signaling pathway. Animal experiment confirmed that PL can alleviate ankle swelling, improve abnormal gait, and down-regulate the protein expression of TNF-α, IL-6, and IL-1β in AGA mice, indicating that PL can treat AGA through TNF-α, IL-6, and IL-1β and the feasibility of network pharmacology to predict drug targets. This study preliminarily discussed the key targets and biological signaling pathways involved in the treatment of AGA with PL combination, which reflected the multi-pathway and multi-target action characteristics of Chinese medicine. Moreover, this study laid a scientific basis for research on the treatment of AGA with PL combination, as well as the mechanism of action.
Animals
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Arthritis, Gouty/drug therapy*
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Drugs, Chinese Herbal/therapeutic use*
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Ligustrum
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Mice
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Network Pharmacology
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Rhizome