Objective To understand the relationship between Hp infection and treatment role in perioperative period patients with liver cirrhosis.Methods Hp in gastric juice and ammonia concentration in blood were examined in 32 patients with liver cirrhosis.Results ⑴The rate of Hp infection was 62 1% in 32 patients with liver cirrhosis.⑵There was no obviously difference in the blood ammonia concentration between both patients with Hp positive and negative.⑶The blood ammonia concentration after Hp eradication was significantly lower than that before eradication.Conclusions Hp infection could partly contribute to occur the hyperammoniaemia in patients with liver cirrhosis.Hp should be checked routinely in perioperative period patients with liver cirrhosis.The eradication of Hp is necessary to prevent and treat the hyperammoniaemia in operative patients with liver cirrhosis.

Objective To investigate the risk factors of progressive intracerebral hematoma (PIH) in severe traumatic brain injury (STBI) and provide reference for diagnosis and treatment of STBI.Methods The clinical information of 96 patients of STBI from March 2008 to March 2012 were retrospectively analyzed,the risk factors of PIH were analyzed by the univariate and multivariate Logistic regression analysis.Results Thirty-six patients occurred PIH,the incidence rate of PIH was 37.5％ (36/96).The univariate regression analysis showed that the age,admission GCS scores,injury to the first CT time,brain contusion,subarachnoid hemorrhage,epidural hematoma,subdural hematoma had certain relation with PIH (P ＜ 0.01or ＜ 0.05).Multivariate Logistic regression analysis showed that the age,brain contusion,subarachnoid hemorrhage,epidural hematoma,subdural hematoma were risk factors of PIH (P ＜0.05 or ＜0.01).Conclusion Fully aware of the risk factors of PIH and timely intervention is the key to reduce the morbidity and mortality of STBI.

Objective To investigate the clinical value of CT scanning combined with ultrasound technique in diagnosis of malignant bone tumor.Methods 100 patients with malignant bone tumor were randomly divided into control group(50 cases with CT scan),and observation group (50 cases received CT scanning combined with ultrasound technology).The diagnostic accuracy rate,tumor bone formation and destruction of bone,periosteal reaction and soft tissue mass were compared between the two groups.All results were judged by the results of MR.Results Diagnostic accuracy rate of the observation group was 80.0％ (40/50),which was significantly higher than 64.0％(32/50) in the control group (x2 =4.65,P ＜ 0.05).In observation group,diagnosis of bone destruction in 60 cases,the tumor bone formation 10cases,83 cases of periosteal reaction and soft tissue mass in 40 cases,those in the control group were 40 cases,5 cases,62 cases,30 cases,the differences between the two groups were statistically significant(x2 =4.86,5.71,6.21,5.22,all P ＜ 0.05).Conclusion The combination of CT and ultrasound in the diagnosis of malignant bone tumors had high diagnostic accuracy rate,and could guide clinical treatment.

Objective To develop a platform of information collection for movements based on ARM7, through which human body movement information can be collected accurately and in time so that deeper scientific research about human body movement can be carried on. Methods By this means, multiplepoint acceleration information can be collected simultaneously through the multinodal network technology, and the information can be processed and retransmitted to computers to be analyzed comprehensively. Results The platform has been used in computer game controlling and good score is given by the tryout person. Conclusion Large quantities of experiment data indicate that this platform possesses higher precision in human body movement information collection and reflect them truthfully. Furthermore, later developments based on this can be applied in fields such as human recovery exercises, physical training, game controlling.

OBJECTIVE:To establish a method for the contents determination of microelements in Chrysanthemum indicum from different production fields. METHODS:Graphite furnace atomic absorption (GFAAS) was used to determine the contents of Pb,Cd and As;HGAAS was used to determination the content of Hg;and FAAS was used to determine the contents of Cu,Fe, Mn,Ca,Mg and Zn. RESULTS:The linear range was 0-50 μg/L for Pb(r=0.999 9),0-10 μg/L for Cd(r=0.999 2),0-50 μg/L for As(r=0.999 0),0-20 μg/L for Hg(r=0.999 5),0-5 μg/L for Cu(r=0.999 3),0-15 μg/L for Fe(r=0.999 8),0-2 μg/L for Mn (r=0.999 9),0-50 μg/L for Ca(r=1.000 0),0-20 μg/L for Mg(r=0.999 9)and 0-2 μg/L for Zn(r=0.999 8);RSDs of precision, stability and reroducibility tests were lower than 3%;recoveries were 94.25%~97.43%(RSD=1.07%)、94.97%~99.46%(RSD=1.68%)、96.25%~99.46%(RSD=1.09%,n=6)、96.61%~99.91%(RSD=1.26%,n=6)、94.11%~98.41%(RSD=1.68%,n=6)、93.11%~99.59%(RSD=2.73%,n=6)、93.11%~99.48%(RSD=2.63%,n=6)、93.01%~99.85%(RSD=2.49%,n=6)、95.13%~99.75%(RSD=1.58%,n=6)、94.08%~97.37%(RSD=1.18%,n=6),respectively. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the contents determination of microelements in C. indicum from different pro-duction fields.

Hepatic failure following partial liver resection is a major complication and a cause of perioperative mortality for patients with primary liver cancer. Today, many different tests have been established for liver function evaluation. These tests contain conventional tests and quantitative tests.Furthermore, we should combine them to precisely evaluate hepatic functional reserve.

Objective: This study explores the applicability of a multi-attribute decision-making method in assessing Guizhi Decoction (GZD) and its varieties as noted in the Shanghanlun. Methods: A known multi-attribute decision-making method, the analytic hierarchy process (AHP), was adopted to transform the clinical challenge of selecting optional decoction for a given combination of symptoms or signs into multiple criteria decision-making problem. Results: A normative model based on the AHP was realized for indications of GZD and its varieties. The indications of sub-family GZD-f (indications of GZD itself) were exterior illness; the indications of GZD-vf1 (indications of GZD's variants which consist of fine adjustments to the ingredients or content on the basis of GZD) were exterior illness with interior excess, suggesting that GZD-vf1 consisted of most conservative variants of GZD; the indications of both GZD-vf2 (indications of Guizhi Qu Shaoyao Decoction and its derivative variants) and GZD-vf3 (indications of Guizhi Gancao Decoction and its derivative variants) showed evolution from exterior illness to interior deficiency in 2 directions. As to efficacy evaluation of the decoction, GZD and its varieties (restricted to those comparable ones) were of equal efficacy on 3 popular signs or symptoms indicating exterior illness: floating pulse, aversion to wind and spontaneous sweating, which were the common ground of indications for the GZD family. Conclusion: Modeling of diagnostic procedure based on the AHP is proved practicable to analyze the clinical judgment system of traditional Chinese medicine. Quantification research on syndrome differentiation and decoction evaluation system focused on signs and symptoms is suggested as a feasible and reliable model.

Peroxisome proliferator-activated receptors (PPARs)are ligand-activated nuclear tran-scription factors,playing an important role in the regulation of glucose and lipids metabolism,inflamma-tion response,proliferation and differentiation.Some drugs targeted on PPARs,such as lipid-lowering and antidiabetic drugs have been developed.Some PPAR agonists were found carcinogenic in animal experi ments,including PPAR αagonist fibrates,PPARγagonist thiazolidinediones,PPARα/γdual ago-nist compounds,and PPARδagonist compounds for clinical development.PPARαagonist carcinogenicity is associated with PPAR receptor activation that regulates lipid metabolis m,and leads to lipids abnormali-ties and increase by peroxisome oxidase in reactive oxygen species (ROS),causing DNA damage. Kupffer cells can generate ROS by NAD PH oxidase that pro motes hepatocyte proliferation and inhibition of apoptosis.PPARγagonist carcinogenicity is generally caused by bladder stone.The carcinogenicity of PPAR agonists to humans has not been confirmed,but the carcinogenic potential of these drugs can-not be ignored.

At present,hepatectomy are recognized as the firsttreatment option for hepatocellular carcinoma (HCC).However,the patients have high frequency of recurrence after operation.In China,Most of the patients with HCC are related to chronic hepatitis B infection.The hepatitis B virus(HBV) factors such as:genotype,status of hepatitis B e antigen,HBV DNA level in serum and HBV DNA level in liver tissue influence the recurrence of tumors.Antiviral therapy,especially interferon therapy may be the effective method to prevent recurrence.HBV status also can influence the recurrence rate after transplant.

Objective In the candidate region 12q13 of simple congenital heart defect(CHD),two single nucleotide polymorphisms(SNPs)in the coding-region of GLI1 gene,rs11553626 and rs2228226,were chosen to investigate their distribution in 180 simple CHD patients and 200 normal controls which were collected in the first affliated hospital,China Medical University and The General Hospital of Shenyang Military Area,in order to determine the relationship between GLI1 gene and simple CHD.Methods Genotypes of these two SNPs were analyzed in 180 simple CHD patients and 200 normal controls by Denatured High Performance Liquid Chromatography(DHPLC)and sequencing from Jan.2000 to Jun.2005.?~2 test was applied to analyze the genotype frequency and allele frequency between CHD groups and control groups.Results No polymorphisms were found at rs11553626 while G/C polymorphisms were found at rs2228226.Remarkable significance was observed at rs2228226 in the allele frequency distribution(?~2=8.956,P