Objective The gene expression profile of gallbladder stone and gallbladder polyp were analyzed by high-throughput sequencing technology to explore the roles of the gene in the development of gallstone and their molecular mechanism in the occurrence and development of gallbladder stone. Methods 7 patients with gallstones and 2 patients with gallbladder polyp was enrolled for RNA-Seq. Results 150 DEGs was identified between gallstones and gallbladder polyps. We found GO terms for molecular functions significantly enriched in antigen binding, while for biological processes, the enriched GO terms were immune response, and for cellular component, the enriched GO terms were extracellular region. The most significant pathway in our KEGG analysis was Cytokine-cytokine receptor interaction (P = 0.000). Conclusion This present study suggests some promising genes may provide a clue to the role of these genes played in the development of gallstones and gallbladder polyps.

Objective To study the correlation of CT perfusion imaging ( CTPI) of induced rat breast cancer with tumor pathological grade and microvascular density ( MVD) ,and the diagnostic value of CTPI for breast cancer. Methods Breast cancer was induced in 35 Wister male rats with intragastric 7,12-dimethyl-1,2-benzanthracene ( DMBA) treatment. Routine breast CT scanning and CTPI were performed. After processed at a workstation,CTPI parameters were obtained including blood flow ( BF) ,blood volume ( BV) ,mean transit time ( MTT) ,permeability surface ( PS) ,and time density curve ( TDC). The rats were sacrificed and breast tumor tisue samples were collected. Breast tumor tisue samples were stained with HE and factor Ⅷ-related antigen immunohistochemistry to observe the pathological type and MVD of tumor. Results Breast tumor was successfully induced in 30 rats ( including 8 with breast hyperplasia and 22 with breast cancer). The BF and BV were higher in rats with breast cancer than in those with breast hyperplasia 151. 72 ? 93. 43 vs 42. 96 ? 32. 42 ml/( min.100 g) ,9. 55 ? 7. 88 vs 2. 17 ? 1. 36 ml/100 g,P 0. 05). The positive correlation of MVD with BF,BV and PS was increased with the decreased tumor differentiation with a correlation coefficient of 0. 701,0. 441 and 0. 521,respectively ( P

Picture archiving and communication system ( PACS ) based on digital teaching platform of medical imaging consists of web teaching system and teaching resource library of medical imaging.The web teaching system includes four function modules of classroom management,work management,teaching management and test management.The teaching resource library includes script,pictures,and audio,video and animation materials of theoretical books,typical cases,examination techniques and medical imaging equipments.Utilized in theoretical and practical classes,the teaching characteristic of medical imaging is made prominent,and the subjective motility of the students is brought into play.

With the development of computer network technology,network teaching has become an important mode of continuing medical education.There are many kinds of medical imaging websites from both domestic and foreign.In general,they can be divided into three main categories,in terms of society official websites,academic journal websites,and educational resource websites.On the basis of the introduction of the representative domestic and international medical imaging websites,this paper analyzes the advantages and disadvantages of them,and emphasizes some measures to improve the application value of them in continuing medical education,including to establish an authoritative academic website to achieve continuing medical education credits,to strengthen the resources and function of various medical image sites,and to promote intelligent and personalized self-learning,etc.We hope the combination of medical imaging websites with continuing medical education will be much closer,so as to provide a good platform for imaging personnel to improve their professional level and scientific research ability.

To observe the chelation of GRP78 with lead (Pb) and its localization changes, astroglial cells from Wistar rat brain were primarily cultured in medium with acetate Pb. The processes were terminated at different time points. The immunoprecipitation (IP) and Western blotting were used for GRP78 purification and expression and the Pb concentration was determined by employing atomic absorption spectrophotometry (AAS). The localization change of GRP78 was observed with colloid gold immunoelectron microscopy. The results showed that the expression of GRP78 was increased significantly in the cells treated with 1.0 mumol/L acetate Pb for 24 h and peaked at 96-192 h (P<0.01), and at the 12th day, the expression of GRP78 began to decrease but was still higher than normal (P<0.05). Pb content started to increase when cells were treated by acetate Pb for 24 h, and the peak appeared at 8 day (P<0.01), and then Pb content decreased gradually, but was still higher than normal (P<0.05). GRP78 protein expression began to remarkably increase when it transferred from ER to the cytosol around the nuclei 24 h after treatment with Pb. It is concluded that GRP78 in astroglia could strongly chelate with Pb ions and it might be a target protein of Pb.

Objective To summarize the imaging features of neurofibromatosis.Methods Imaging data of 7 patients with neurofibromatosis proven by operation and clinical syndrome,were retrospectively analyzed.Results In 7 cases of neurofibromatosis,the findings of CT and MRI displayed 3 cases of bilateral vestibular schwannoma,2 of which were combined with optical nerve glioma;1 case of optical nerve glioma complicated with neurofibromatosis under cervical dura;1 case of meningioma and 2 cases with the changes of dura spinal sac.The last two cases displayed abnormal skull and spinal canal,concave change of vertebra and enlarged dura spinal sac,partially stretched to never root on CT plain flim and MRI.Conclusion Neurofibromatosis can be definitely diagnosed by optimal imaging examination based on comprehensive analysis of imaging features and typical syndromes.

This paper introduced the current situation of WeChat's official accounts of medical imaging.According to the operation agents,they can be divided into different categories,including professional societies,hospitals and departments,medical companies,academic journals,medical imaging websites and personal accounts.This paper also analyzed the advantages and disadvantages of WeChat's official accounts of medical imaging.Some measures were proposed to improve their application value in continue medical education (CME),such as establishing a platform of WeChat's official account with high approval and authority to award CME credit,integrating various kinds of network resources,strengthening the abundance,systematicness,chronergy and practicality of the resources,setting up and improving the evaluation mechanism of CME credit award,so as to provide a CME platform for the staff of medical imaging.

OBJECTIVE:To investigate the effects of total paeony glycoside (TPG) on the expression of tumor suppressor gene in lung cancer model rats. METHODS:90 rats were randomly divided into normal group,model group,positive control group [cyclophosphamide,50 mg/(kg·d)] and TPG low-dose,medium-dose and high-dose groups [50,100,200 mg/(kg·d)] with 15 rates in each group. Except for normal group,other groups were given disposable infusion of carcinogenic iodized oil via left lobe bronchus to induce lung cancer model. After modeling,those groups were given relevant medicine intravenously from Monday to Friday,while normal group and model group were given constant volume of normal saline intravenously for consecutive 16 weeks. The expression of multidrug resistance associated protein(MRP),human multidrug resistance gene(MDR1),P21 and P16 mRNA in lung tissue of rats were determined by RT-PCR;the expression of P53 protein in lung cancer tissue was determined by IHC method.The rate of positive expression was calculated,and pathological change of lung tissue was observed. RESULTS:Com-pared with normal group,the expression of MRP,MDR1,P21,P16 mRNA and P53 protein(positive rate of 66.67%)in lung tis-sue was increased significantly in model group (P<0.05);compared with model group,the expression of MRP,MDR1,P21, P16 mRNA and P53 protein (positive rate of 46.67%,46.67%,40.00%,13.33%) decreased in positive control group,TPG low-dose,medium-dose and high-dose groups in dose-dependent manner,and the decrease of TPG medium-dose and high-dose groups were more significant than that of positive control group (P<0.05);there was statistical significance in above indexes among TPG groups(P<0.05). CONCLUSIONS:TPG could inhibit the expression of MRP,MDR1,P21,P16 gene and P53 pro-tein in lung cancer model rats significantly.

Objective To explore the clinical value of radiological equipment for injury treatment in earthquake. Methods s One hundred and forty-three cases of the injury in earthquake using radiological equipment were analyzed retrospectively. Resultss All of the cases were performed X-ray plain film examination, CT in 78 cases and MRI in 19 cases. Imaging examination could establish definitive diagnosis in 143 cases of the injury in earthquake. Conclusion Radiological equipment is the most valuable examination equipment in diagnosis of the injury in earthquake.

Objective To investigate changes in bone mineral density (BMD) and analyze its related factors in community populations to provide the early diagnosis of osteoporosis (OP) and give right guidance to prevent osteoporosis. Methods The quantitative ultrasound BMD analyzer was used to measure BMD of heel in 8 711 adults in community. At the same time a questionnaire survey was conducted among these subjects. The data were analyzed by Logistic stepwise regression analysis. Results With the age changing, males and females tended to have different BMD variation. Male and female's BMD peak values were respectively in 35-age group, 30-age group and decreased as age increased. T value of BMD was different between male and female (-1.40(-2.0--0.7)vs-1.3(-2.0--0.5))(P<0.001). Both men and women had a higher incidence of low bone mass (53.34%vs 47.46%), and the difference was significant (P<0.001), whereas the incidence of osteoporosis after 55 years of age between men and women was statistically significance (P<0.05). BMD was the lowest for female than for male in 50-age group, and the prevalence rate of OP was remarkably increased. Analysis of related factors of BMD showed that body mass index (BMI), age, diabetes and menopause were risk facts. Conclusion The BMD among adults is related to many factors, in which the age, BMI and menopause are the most important factors. Much attention should be paid to low bone mass phenomenon, timely monitoring, timely intervention, develop healthy working and living habits, is important to the prevention of osteoporosis and its complications.