Objective To investigate effect of folic acid combined with xin funing on CRP, HGF, IL-2,TNF-αof patients with cervical cancer caused by human papillomavirus.Methods 80 cases of cervical cancer patients were randomly divided into control group, 40 cases in the control group were given conventional chemotherapy, 40 cases in the experimental group were on the base of the control with folic acid combined with xin funing.CRP, HGF, TNF-α, IL-2 and T lymphocyte subsets were compared before and after the treatment.Results Compared with the control group, the serum CRP, HGF and TNF-αof the experiment group were lower(P<0.05), IL-2 levels was higher (P<0.05), CD4 +and CD4 +/CD8 +level were higher(P<0.05), level of CD8 +was lower(P<0.05) and the clinical effective rate were higher(P<0.05).Conclusion Folic acid combined with Xin Funing has important significance for the treatment of patients with cervical cancer.It is speculated that the mechanism may be to reduce the level of serum CRP and HGF in patients with cervical cancer, and to increase the level of IL-2, and to regulate immune cells.

OBJECTIVE:To study the effects and mechanism of rapamycin on invasion and metastasis of cervical cancer HeLa cell. METHODS:HeLa cells were divided into control group and rapamycin low-dose,medium-dose and high-dose groups (10, 30,100 nmol/L). After treated for 48 h,cell viability was measured by MTT assay,and inhibitory rate was calculated;migration and invasion of cell was tested by Transwell assay. The expression of matrix metalloproteinase 2(MMP-2),MMP-9,Vimentin and E-cadherin,and phosphorylation of protein kinase B (Akt),mammalian target of rapamycin (mTOR) were detected by Western blot. RESULTS:Compared with control group,the inhibition rate of cell viability was increased in rapamycin groups(P<0.01);the number of invasion and metastasis cells decreased(P<0.01);the expression of MMP-2,MMP-9 and Vimentin were decreased (P<0.01 or P<0.05);the expression of E-cadherin was enhanced(P<0.01 or P<0.05);the phosphorylation of Akt and mTOR were reduced (P<0.01). CONCLUSIONS:Rapamycin could inhibit invasion and metastasis of HeLa cell via Akt/mTOR signal pathway.

Objective To evaluate the value of ultrasound in diagnosing shoulder lesions.Methods Ultrasound images were obtained by two museuloskeletal sonologists in 111 patients suspected of having rotator cuff and non-rotator cuff lesions which were confirmed by following arthroscopic surgery.Results The arthroscopic findings demonstrated that there were two or more lesions in one patient in majority of patients,included 67 supraspinous tendon tears,5 infraspinous or subscapular tendon injuries,10 calcific tendonosis,2suprascapular notch cysts,34 subaeromial bursitis,1 intrabursa loose body,13 biceps longus tendon dislocation or disruption,and 21 lahrum injury.The accuracy of ultrasonogrpahy was 92% in diagnosis of supraspinous tendons tear,97% in subscapular tendon inj uries,96% in calcific tendonitis,1 00% in suprascapular notch cysts,88% in subacromial bursitis,100% in biceps longus tendon dislocation or disruption,and 81% in labrum injury.Conclusions Ultrasound can evaluate shoulder lesions effectively.

Objective To investigate the incidence,sonographic appearance of the anatomic variation of carpal tunnel median nerve and its accompanying structures in healthy volunteers and explore the value of this variation in carpal tunnel syndrome.Methods A total of 360 hands of 180 healthy volunteers were included in the study.The full course of the median nerve in the forearm and carpal tunnel was examined with high-frequency ultrasound.The median nerve was first located in cross section at wrist and then with continuous cross-sectional scanning to observe the the full course of the median nerve in the forearm and carpal tunnel with high-frequency ultrasound.Results Anatomic variation of carpal tunnel median nerve and its accompanying structures were observed:① High division median nerve were found in 2 wrists (0.56 ％) ;②Bifid median nerve were found in 17 wrists (4.72％) ;③Persistent median artery were found in 22 wrists (6.11％),and 2 wrists (0.56％) were also found accompanied vein.Aanatomic variation of carpal tunnel median nerve accompanied with persistent median artery were observed in 16 wrists (4.44 ％).Conclusions High-frequency ultrasound was sensitive to diagnose the anatomic variation of carpal tunnel median nerve and its accompanying structures.Recognition of these variations can help us to make correct diagnosis of carpal tunnel syndrome.

Objective To learn how the process of self-regulatory fatigue (SRF) mediates the effect of leadership styles on job satisfaction of young workers.Methods 723 young workers recruited from a business enterprise were required to response to questionnaire survey, including leader behavior description, self-regulatory fatigue and job satisfaction questionnaire.Results All young workers' job satisfaction was 3.78±0.69,31 years old and above were higher than 30 years old and under(t=-3.246, P＜0.01) ,and there was no significant difference in gender and education.Job satisfaction was significantly positively correlated with leadership style (r initiating structure =0.210, r ideration =0.272), and was significantly negatively correlated with self-regulatory fatigue (r=-0.333).Two dimensions of leadership styles,initiating structure (β=0.227, t=2.659, P＜0.01) and consideration (β=0.270, t=5.035, P＜0.01), had the predicted effects of job satisfaction (R 2 =0.098).Consideration dimension of leadership style had more influence on job satisfaction at the high level of self-regulatory fatigue (β=0.266, t=4.929, P＜0.01);the effect of initiating structure dimention on job satisfaction was influenced by gender (t=-5.154, P＜0.01);self-regulatory fatigue was mediator between leadership styles and job satisfaction, and the consideration dimension of leadership style had more mediation effect on job satisfaction than that of initiating structure dimention of leadership style (mediationconsideration=0.317,mediationinitiating structure=0.268).Conclusion Two dimensions of leadership style can affect job satisfaction, and are mediated by self-regulatory fatigue.The intervention of self-regulatory fatigue can help to improve the job satisfaction of young workers.

Objective:To evaluate the reliability and validity of the Chinese version of the Self-Regulatory Fa-tigue Scale (SRF-S)in Chinese young people.Methods:A sample of 553 young people from a university and a state-owned enterprise was administrated with the SRF-S,the Simplified Coping Style Questionnaire (SCSQ)and the Dual-Mode Self-Control Scale (DMSC-S).Item analysis was conducted to identify valid items and revise the scale.Then the construct validity of the revised scale was tested through confirmatory factor analysis.Finally,a sam-ple of 238 graduate students was retested two weeks later to examine the reliability of the scale.Results:Item analy-sis showed that the SRF-S had satisfactory item discrimination (r >0.38).Confirmatory factor analysis showed that the whole scale had a three-order factor and good construct validity (χ2 /df=5.08,RMSEA =0.09,NFI =0.90, NNFI =0.90,CFI =0.92,IFI =0.92,GFI =0.90)after removing 2 items with factor loadings lower than 0.32,and the range of factor loading was between 0.37 and 0.71.The SRF-S total and subscale scores were positively corre-lated with the scores of negative coping and poor control (r =0.25 -0.58,Ps <0.001),and were negatively associ-ated with the scores of positive coping and good self-Control (r =-0.22 --0.47,Ps <0.001).The Cronbach's αcoefficients were 0.84 for the total scale,and 0.64 to 0.69 for the three subscales,respectively;the test-retest relia-bility coefficients were 0.73 for the total scale,and 0.62 to 0.67 for the three subscales,respectively.Conclusion:It suggests that the Chinese version of SFR-S could be used as an effective and reliable tool for assessing self-regu-latory fatigue state among Chinese young people.

OBJECTIVETo study the effect and possible impact mechanism of salidroside on cognitive ability of Alzheimer's disease (AD) model rats induced by amyloid beta peptide (Abeta1-40).

METHODAbeta1-40 was injected into bilateral hippocampus to create the AD model. Afterwards, different doses of salidroside (25, 50, 75 mg x kg(-1)) were orally administered for 21 days. Rats' learning and memory abilities were detected by Morris water maze testing system. The levels of the superoxide dismutase (SOD), malondialdehyde (MDA), and the expression of nuclear factor-kappaB (NF-kappaB), inducible nitric oxide synthase (iNOS) and receptor for advanced glycation end products (RAGE) protein in hippocampus were also detected by different methods.

RESULTThe place navigation test showed longer escape latency, low frequency of platform quadrant crossing per unit time, damage in learning capacity, significant decrease in SOD acivity in hippocampus, notable increase in MDA content, NF-kappaB, iNOS and RAGE protein expressions in rats. Salidroside (50, 75 mg x kg(-1)) significantly alleviated the impairments of learning and memory ability. The activity of SOD increased in salidroside (50 droside group compared with that of the Alzheimer's disease group (P < 0.01).

CONCLUSIONSalidroside may treat Alzheimer's disease by inhibiting the oxidative stress.

Alzheimer Disease ; drug therapy ; Amyloid beta-Peptides ; toxicity ; Animals ; Cognition ; drug effects ; Disease Models, Animal ; Glucosides ; pharmacology ; therapeutic use ; Male ; Maze Learning ; drug effects ; NF-kappa B ; metabolism ; Nitric Oxide ; physiology ; Phenols ; pharmacology ; therapeutic use ; Rats ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic ; analysis ; Superoxide Dismutase ; metabolism

Objectives To investigate the image quality,influencing factors and radiation doses of prospectively ECG-triggered spiral acquisition mode (Flash spiral mode)coronary computed tomography angiography (CCTA) using high-pitch dual-source CT in patients with different heart rates.Methods One hundred and thirty-four consecutive patients with mean heart rate (HR) ＞ 65 beats per minute (bpm) and ≤ 100 bpm were included in this study as group A using Flash spiral mode setting at 20％ -30％ of the R-R interval and 134 consecutive patients with mean HR ≤65 bpm were enrolled as group B using Flash spiral scan mode at 55％ of the R-R interval; 134 consecutive patients with mean HR ＞ 65 and≤100 bpm using spiral scan mode were included as group C. The image quality scores, effective radiation dose and influencing factors of image quality in three groups were assessed. All statistical analyses were performed using SPSS.Results (1)The non-diagnostic coronary artery segments in group A (28/1842,1.52％) were more than in group B (8/1819,0.44％ ) ( x2 =10.97,P =0.001 ) and there was no significant difference between group A and group C (32/1838,1.74％) ( x2 =0.280,P =0.345).The number of patients with non-diagnostic coronary segments in group A( 10/134,7.5 ％ )was more than in group B (2/134,1.5 ％,x2 =5.52,P =0.018 ),while there was no significant difference between group A and group C (9/134,6.7％,x2 =0.057,P =0.812).(2)The average heart rate variability (HRV) of patients with different image scores in the three groups wcrc significantly different. In group A,the HRV of score 1,2 and 3 were (2.29 ± 1.06),(5.17 ± 1.37),(8.88 ± 1.53) bpm,respectively (F =170.402,P =0.001 ).In group B were (2.26±1.01),(5.97 ±1.82),(12.00 ±9.64) bpm,respectively (F=95.843,P=0.001).In group C were (2.61 ±1.85),(7.90 ±3.97),(11.22 ±5.62) bpm,respectively (F=68.629,P=0.001 ). (3) The average effective radiation doses in groups A and B were significant lower than in group C [ A group was 1.04 ± 0.16,B group was 1.03 ± 0.16 and C group was 7.05 ± 1.05,t =65.5 ( A vs C),P ＜0.01 ( A vs C) ].Conclusions Flash spiral mode of high-pitch dual-source provides high image quality with significant reduction of radiation exposure in patients with HR ≤ 65 bpm. Patients with heart rates ＞65 bpm and ≤100 bpm without cardiac arrhythmia can perform CCTA using Flash spiral mode with image acquisition time setting at 20％-30％ of the R-R interval.

Objective To investigate the relationship between lipoprotein-associated phospholipase A2(Lp-PLA2) activity and the severity of coronary artery diseases .Methods A case-control study was conducted to select 231 patients with positive coronary angiography results in Beijing Huaxin Hospital .They were divided into two groups (untreated goup:147 cases; the medication group:84 cases) according to whether taking statins.99 cases were included in the healthy control group .In the untreated group, all male patients were grouped according to the number of diseased coronary branches (43 cases were in single vessel lesion group and 52 cases were in multi vessel disease group ) or to the Gensini score (32 cases were in low score group, 36 cases were in middle score group and 27 cases were in high score group ).The clinical data were collected by detecting serum atherosclerotic markers such as Lp-PLA2, total cholesterol (CHOL), low-density lipoprotein (LDL).The number of vessels involved in coronary artery disease , the position of the lesion and the degree of stenosis were confirmed by percutaneous coronary intervention ( PCI).Statistical evaluations were performed using t-test, variance analysis, Mann-Whitney U test and Spearman correlation analysis.Results The differences among untreated group , the medication group and the control group were statistically significant, of which the Lp-PLA2 level was (561.9 ±158.5) U/L, (373.2 ±124.7) U/L and (467.4 ±130.4) U/L respectively.Compared with single branch disease group , the Lp-PLA2 level in the multiple branches disease group was increased dramatically .Additionally, the level of Lp-PLA2 in high Gensini score group was (618.7 ±165.4) U/L, significantly higher than the low score group (517.3 ± 191.7) U/L.Conclusion Lp-PLA2 activity was associated with the severity of Coronary Artery Diseases, which can provide the evidence for the follow-up treatment programs.

Objective: To observe the relationship between impaired myocardial untwisting and left ventricular diastolic dysfunction in patients with autoimmune diseases (AD). Methods: In this retrospective study, 95 AD patients (27 males, (38.6±14.2) years old) were enrolled as AD group and 71 gender and age matched healthy subjects (24 males, (37.6±12.2) years old) were enrolled as control group, all underwent transthoracic echocardiography and two-dimensional speckle-tracking echocardiography (STE) in our hospital between January 2014 and June 2018. Left ventricular untwisting and diastolic function parameters were measured. Multiple logistic regression analysis was used to identify related factors of left ventricular diastolic dysfunction in AD patients. Receiver operating characteristic (ROC) curve was used to identify the diagnosis value of untwisting parameters for left ventricular diastolic dysfunction in AD patients. Results: Compared with control group, left ventricular ejection fraction was lower (58(47, 66)% vs. 67 (62, 71) %, P<0.001), E/e′ was higher (10.78 (7.28, 13.65) vs. 6.30 (5.55, 7.25) , P<0.001), isovolumic relaxation time was longer (73.5 (56.5, 88.0) ms vs. 62.0 (58.0, 68.5) ms, P<0.001),and untwist slope during isovolumic relaxation period (USIR) was lower (31.92 (14.09, 54.92) °/s vs. 59.90 (40.09, 87.18) °/s, P<0.001) in AD group than in control group. Multiple logistic regression analysis showed heart rate (OR=0.885, 95%CI 0.840-0.931, P<0.001), E/e′ (OR=0.655, 95%CI 0.537-0.798, P<0.001) and USIR (OR=0.986, 95%CI 0.974-0.998, P=0.020) were independently related with left ventricular diastolic dysfunction in AD patients. ROC curve showed that area under the curve (AUC) was 0.919 (P<0.001), sensitivity was 87.6%, and specificity was 88.7%, when combining the heart rate, E/e′, and USIR as assessment parameters for the diagnosis of left ventricular diastolic dysfunction in AD patients at a cutoff of 0.51. Conclusions Impairment of myocardial untwisting indicates the presence of early stage left ventricular diastolic dysfunction in AD patients. USIR may be a sensitive parameter to evaluate early stage left ventricular diastolic dysfunction in AD patients.