Objective To explore the application value of neutrophil to lymphocyte subsets ratios in clinical staging and pathological classification of non-small cell lung cancer(NSCLC).Methods A total of 132 NSCLC patients who were hospitalized and diagnosed by histopathology from January 2018 to December 2020 in the hospital were selected as the NSCLC group.Another 85 healthy individuals who underwent physical ex-aminations at the same hospital were selected as the control group,and the neutrophil to lymphocyte subsets ratios of the two study groups were detected and calculated for comparison.Results Compared with the con-trol group,the CD3+T cell,CD4+T cell,CD8+T cell,B cell,natural killer(NK)cell,and CD4+/CD8+in the NSCLC group were significantly reduced,while neutrophil to lymphocyte ratio(NLR),neutrophil to CD3+T cell ratio(N/CD3+),neutrophil to CD4+T cell ratio(N/CD4+),neutrophil to CD8+T cell ratio(N/CD8+),neutrophil to B cell ratio(N/B),and neutrophil to NK cell ratio(N/NK)significantly increased(P＜0.05).Compared with adenocarcinoma group,NLR,N/CD3+,N/CD4+,N/B.and N/NK were higher in the squa-mous cell carcinoma group(P＜0.05).NLR,N/CD3+,N/CD4+,N/CD8+,N/B and N/NK in the stage Ⅰgroup,the stage Ⅱ group,the stage Ⅲ group and the stage Ⅳ group were significantly higher than those in the control group(P＜0.05).Compared with the stage Ⅰ group,N/CD3+,N/CD4+,N/CD8+,and N/B in the stage Ⅲ group and the stage Ⅳ group were significantly increased(P＜0.05),while CD3+T cell and B cell were significantly decreased(P＜0.05).Receiver operating characteristic curve results showed that the area under the curve and Youden index of the neutrophils to lymphocyte subsets ratios were higher than those of the corresponding lymphocyte subsets.The Linear Support Vector Classification(Linear SVC)algorithm based on machine learning was used to construct a NSCLC risk prediction model using N/CD3+,N/CD4+,N/CD8+,N/B,and N/NK indicators.The results showed that the area under the curve could reach 0.929,with a positive predictive value of 0.911 and a negative predictive value of 0.787.Conclusion The neutrophil to lym-phocyte subsets ratios are closely related to the clinical staging and pathological classification of NSCLC.The combined detection of N/CD3+,N/CD4+,N/CD8+,N/B,and N/NK has high clinical diagnostic efficacy and could provide new diagnostic and therapeutic ideas for the early diagnosis and assessment of the severity of NSCLC.

Objective To analyze the detection results and distribution characteristics of serum allergen-specific immunoglobulin(Ig)E antibodies in patients with type Ⅰ allergic diseases(hereinafter referred to as allergic diseases)in Chongqing,and to provide epidemiological data and diagnostic evidence for the prevention and treatment of allergic diseases.Methods A total of 9189 patients who underwent allergen-specific IgE an-tibody detection in this hospital from January 2023 to February 2024 were included in the study.The positive cases of allergen specific IgE antibodies detection were statistically analyzed according to age groups,genders,and sea-sons,and the distribution characteristics and patterns of specific allergens were analyzed.Results Among the 9 189 patients with allergic diseases,4 143 cases showed positive allergen-specific IgE antibodies detection results,with a positive rate of 45.09％.The proportion of patients with multiple sensitization was 68.53％.The main inhalant allergens included Dermatophagoides pteronyssinus(28.95％),Dermatophagoides farinae(23.62％),and Blomia tropicalis(10.95％);while the main ingested allergens were milk(7.57％),shrimp/crab(2.84％),and egg white(1.98％).The positive rate of inhalant allergens was higher than that of ingested al-lergens.The positive rate of allergens in males was higher than that in females(P＜0.05).The allergens in infants and toddlers under 3 years old were mainly ingested and mostly sensitized by single allergen.The posi-tive rate of allergens and multiple sensitization rate were the highest in patients aged 12-＜18 years.The pos-itive rate of allergens was the lowest in patients over 60 years old.The positive rate of allergens was the high-est in autumn,and Dermatophagoides pteronyssinus and Dermatophagoides farinae were the main allergens in all seasons.Conclusion Patients with allergic diseases in Chongqing exhibited the following characteristics:Dermatophagoides pteronyssinus and Dermatophagoides farinae are the main allergens,the positive rate of al-lergens in males is higher than that in females,the majority patients are 12-＜18 years old,and the disease is more prone to occur in autumn.These findings can provide a reference for the prevention and treatment of al-lergic diseases in this region.