1.Interventional therapy of traumatic pseudoaneurysm
Linsun LI ; Haibin SHI ; Lifu WANG
Journal of Interventional Radiology 1994;0(02):-
Objective To introduce the different therapeutic methods for the different types of traumatic pseudoaneurysms. Methods Traumatic pseudoaneurysm was treated in 8 patients. Intraarterial embolization was performed with gelfoam in 3 patients for terminal type. Endovascular covered stent graft was inserted in 2 patients with trunk type. One patient with branch type, we used metallic coils to embolize both broken ends. One patient with perforation in femoral artery was treated with compression. For 1 patient with huge haematoma and pseudoaneurysm, operation for elimination the haematoma was performed after embolization. Results Pseudo- aneurysm kept closed after treatment achieved in all 8 patients. Distal arterial pulse was normal in 2 patients treated with stent graft. A new pseudoaneurysm presented as a complication at puncture site in 1 patient due to other factors. There were no other symptomatic complications. Conclusions Pseudo- aneurysms can be treated effectively with interventional techniques instead of surgical operation.
2.Percutaneous fenestration for ischemic complications of aortic dissection
Linsun LI ; Rixing XU ; Lifu WANG
Journal of Interventional Radiology 2001;0(06):-
Objective The evaluation and possibility of percutaneous fenestration for ischemic complications of aortic dissection were disccused. Methods A male patient with aortic dissetion (type: DeBakey Ⅲb) accompanyed by lower extremities pararises was undertaken percutaneous fenestration. Results Both the lower leg's blood flow was recovered with symptom free. Bilateral femoral and dorsal foot pulsations could be feeled. Conclusion Percutaneous fenestration for ischemic complications of aortic dissection is a safe and effective management, but should be done earlier.
3.Establishing a system of therapeutic effect evaluation reflecting the theory of body and spirit
Yajun ZHANG ; Youlin LI ; Lisheng HU ; Wei WANG ; Lifu BI
China Journal of Traditional Chinese Medicine and Pharmacy 2006;0(09):-
In this article,the authors analyzed the current status of the therapeutic effect evaluation of TCM and proposed some measures to improve the current therapeutic effect evaluation of TCM,quantificated messages from TCM four diagnostic methods,explored biological parameter reflected essence of diseases,introduced quality of life scale and PRO,in the hope of establishing a comprehensive system of therapeutic effect evaluation reflecting the superiority and characteristics of theory of body and spirit.
4.A research into the origin and development of TCM view of physique and spirit
Yajun ZHANG ; Youlin LI ; Lisheng HU ; Wei WANG ; Lifu BI
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(10):-
The article searched ancient literature from pre-Qin to Qing Dynasty,researched the character meaning,implication of physique and spirit,and the historical evolvement process of view of physique-spirit unity(ghost theory-sporadic physique and spirit theory-systemic view of physique-spirit unity-view of physique-spirit unity combined with Chinese medicine)so that the view of physique and spirit can be applied to medical domain preferably,which will help the clinic diagnosis and treatment.
5.Construction and application of network curriculum of health management subjects
Ting LI ; Lulu ZHANG ; Jinhai SUN ; Lifu CHEN
Chinese Journal of Medical Education Research 2005;0(06):-
It is an important part of curriculum resources for network curriculum. An excellent network curriculum can enhance the quality of curriculum resources. In our teaching practice of health management,we designed and constructed network curriculum according to the characteristics of subjects and demand of our students based on the public net platform of health management.
6.Nursing mode exploring of treating closed hepatic trauma and hemorrhage by percutaneous coronary intervention in field minimally-invasive interventional shelter
Fei LI ; Jingjing RONG ; Ming LIANG ; Jingyang SUN ; Kai GUO ; Lifu NA ; Yaling HAN
Chinese Journal of Practical Nursing 2016;32(22):1712-1714
Objective To explore the nursing mode of percutaneous coronary intervention (PCI) in field minimally- invasive interventional shelter for treating closed hepatic trauma and hemorrhage. Methods 8 animal modes of closed hepatic trauma were established by beagles. Then, the animals with damaged hepatic arteries were treated by emergency angio-interventional embolic treatment. And specific nursing mode, which was different from in-hospital, was performed in perioperative period. Results All the animal modes of closed hepatic trauma and hemorrhage were rescued successfully, and no operative complications were found. Conclusions With the cooperation of specific nursing mode, the emergency angio-interventional embolic treatment of closed hepatic trauma and hemorrhage in field minimally-invasive interventional shelter under the complex outdoor environment is feasible.
7.Influence ofZhulian acupuncture exciting method on hypoxic ischemic brain damage in rats to oxidative stress
Lirong CHEN ; Xiaoxia PAN ; Mingming CHEN ; Fawen ZHENG ; Jianhua FAN ; Yanjing LI ; Lifu WEI
International Journal of Traditional Chinese Medicine 2016;38(3):238-241
Objective To observate theZhulian acupuncture exciting method in different time of hypoxia ischemia brain injury in rat brain tissue malondialdehyde (MDA), monoamine oxidase (MAO), nitric oxide (NO) and glutathione peroxidase (GSH-PX) content.Methods 7 days old rats were randomly divided into a excitation method acupuncture group I, a excitation method acupuncture group II, a model group, a sham operation group, a normal control group, 10 rats in each group. Excitation method acupuncture group I and normal control group were given stimulation ofZhulian acupuncture exciting method one type technique from 24 h model manipulation, excitation method acupuncture II group from the beginning of the eighth day given Stimulation ofZhulian acupuncture exciting method one type technique. The sham operation group and the model group were not treated by acupuncture. The animals were sacrificed at the twenty-first day after making the model, determined brain tissue MDA、MAO、NO and GSH-PX Content.Results Compared with the model group, MDA (3.4 ± 0.87 nmol/mgvs. 5.50 ± 1.58 nmol/mg) content decreased in the excitation method acupuncture group I (P<0.05). The NO (12.43 ± 3.47μmol/mgvs. 17.10 ± 5.82μmol/mg) content decreased in the excitation method acupuncture group II (P<0.05). MAO (32.12 ± 11.15 U/mg, 31.01 ± 9.92 U/mgvs. 40.90 ± 11.02 U/mg) content were decreased in both excitation method acupuncture group I and group II (P<0.05), while the GSH-PX (2.61 ± 1.20 U/mg, 2.61 ± 1.37 U/mgvs. 1.43 ± 0.49 U/mg) content were increased (P<0.01). ConclusionZhulian acupuncture exciting method one type technique can decrease the content of MDA, MAO and NO reduce the content of hypoxic-ischemic brain injury in rat brain tissue, increase the content of GSH-PX, promote the removal of immature rats with hypoxic ischemic brain damage brain tissue metabolism, and protect brain function.
8.Effect of Zhu Lian Acupuncture Exciting Method on Nerve Cell Apoptosis and the Expressions of PI3K, AKt and Caspase-3 Proteins in Young Rats with Hypoxic-ischemic Brain Damage
Mingming CHEN ; Xiaoxia PAN ; Fawen ZHENG ; Yanjing LI ; Jianhua FAN ; Lifu WEI
Shanghai Journal of Acupuncture and Moxibustion 2016;35(5):592-595
Objective To investigate the effect of different time intervention of Zhu Lian acupuncture exciting method on nerve cell apoptosis and tissue expressions of PI3K (phosphatidylinositol 3-kinase), AKt (serine/threonine kinase) and Caspase-3 (cysteinyl aspartate specific proteinase-3) proteins in young rats with hypoxic-ischemic brain damage.Methods Fifty 7-day-old rats were randomized into groups A (acupuncture exciting methodⅠ), B (acupuncture exciting methodⅡ), C (model), D (sham operation) and E (normal control), 10 rats each. Groups A and E began to receive acupuncture in 24 hours after model making and group B, at 8 days after model making. Groups C and D were not given acupuncture. Every group of animals was sacrificed at 21 days after model making. Nerve cell apoptosis was examined using In situ end labeling (TUNEL) technique. Cerebral expressions of PI3K, AKt and Caspase-3 proteins were determined by immunohistochemical method.Results The number of apoptotic cells was significantly smaller in groups A and B than in group C (P<0.01,P<0.05) and decreased significantly in group A compared with group B (P<0.05). The expressions of PI3K and AKt proteins increased significantly and the expression of Caspase-3 protein decreased significantly in groups A and B compared with group C; there were statistically significant differences (P<0.01,P<0.05). PI3K expression increased significantly and Caspase-3 protein expression decreased significantly in group A compared with group B (P<0.05); there were statistically significant differences (P<0.05).Conclusions Zhu Lian acupuncture exciting method can inhibit nerve cell apoptosis, stimulate the expression of PI3K/AKt signaling pathway, increase PI3K and AKt activities and reduce the expression of Caspase-3 protein in young rats with hypoxic-ischemic brain damage. Early intervention of Zhu Lian acupuncture exciting method is of important significance in producing a protective effect on brain nerves in young rats with hypoxic-ischemic brain damage.
9.Combine traditional Chinese and western medicine treatment of chronic stomach disease clinical efficacy research
Yong XIAO ; Yujie PAN ; Qi XING ; Wei LI ; Lifu JIANG
Journal of Pharmaceutical Practice 2015;(5):460-462,476
Objective To investigate the effectiveness of the Weishule granule therapy with rabeprazole particles associ-ated chronic stomach .Methods 488 cases of chronic gastritis were divided into treatment group and control group .Control group:oral rabeprazole enteric-coated capsules ,1 time/d ,20 mg/times ;treatment groups :on the basis of additional services Weishule granules ,3 times/d ,10 g/times ,three weeks for a course respectively .The efficacy of two diseases ,endoscopy and TCM syndromes were compared .Results The treatment effect among functional dyspepsia ,gastritis treatment group and the control group had statistically difference (P<0.01);The treatment effect between atrophic gastritis treatment group and the control group was statistically difference (P<0.05);The efficacy of endoscopic lesions between the treatment group and the control group was statistically difference (P<0.05) .TCM syndrome ,the liver and stomach damp-heat syndrome ,Fan Wei qi syndrome treatment group and the control group ,had statistically difference (P<0.01) ,stomach meridian blood stasis ,spleen deficiency syndrome ,cold and heat mixture syndrome treatment group and the control group had statistically difference (P<0.05) .Conclusion The effective rate and cure rate of Weishule granule combined rabeprazole azole in patients with chronic stomach trouble was better with less adverse reactions .
10.Morphological observation of Tritrichomonas muris isolated from Xinjiang Cricetulus migratorius and analysis of its 16 S rRNA gene
Shen SHI ; Gang YAO ; Cheng YUE ; Li BAI ; Lifu LIAO ; Yimei XU ; Yan ZHANG ; Ying DING ; Shunsheng YAN
Acta Laboratorium Animalis Scientia Sinica 2014;(4):28-30
Objective To conduct morphological observation and gene identification of the strain of flagellate iso -lated from Cricetulus migratorius in the Xinjiang Research Center for Experimental Animals .Methods The ileocecal con-tents of C.migratorius were microscopically examined on direct smear with Wright-Giemsa staining , and the total RNA iso-lated from Xinjiang C.migratorius was extracted and 16S rRNA was amplified by PCR , and then sequenced .Furthermore the homology was compared and the phylogenetic tree was developed using MEGA 5.22 software.Results Morphological observation indicated that the isolated flagellate was Tritrichomonas muris.The 16S rRNA gene sequence of the Xinjiang C. migratorius isolate shared highly homology with that of other Tritrichomonas.Phylogenetic tree analysis indicated that the 16S rRNA gene of Xinjiang C.migratorius isolate was classified into a subgroup with T.muris 16S rRNA (U85966.1), but was relatively distant relative from other related tritrichomonas.Conclusions The flagellate isolated from Xinjiang C. migratorius is identified to be T.muris by both morphological observation and 16S rRNA gene analysis.