BACKGROUND:Currently, fibroblast-like synoviocytes from the synovial tissue of patients with rheumatoid arthritis or adjuvant arthritis rats are isolated and cultured mainly using enzyme digestion method, but tissue explant adherent method is rarely reported in primary culture. OBJECTIVE:To establish a culture method of fibroblast-like synoviocytes from adjuvant arthritis rabbits and to investigate cell biological characteristics. METHODS:Rabbit models of adjuvant arthritis were prepared by injected with adjuvant at multiple sites and times, the knee synovial tissues were obtained, and fibroblast-like synoviocytes were separated and cultured by the tissue explant adherent method. Furthermore, the cell morphology was observed. The cell proliferation was measured using cell counting kit-8 assay, and the expression of Vimentin was detected by immunofluorescence.RESULTS AND CONCLUSION:The primary cultured rabbit synoviocytes were long spindle fiber-like and coincided with the fibroblast-like synoviocytes. Moreover, cells were positive for Vimentin. In conclusion, fibroblast-like synoviocytes from rabbits with adjuvant arthritis are successfully isolated and cultured by the tissue explant method.

Objective To investigate the clinical efficacy of conventional treatment combined with flupentixol and melitracen in patients with reflux esophagitis (RE).Methods From June 2012 to March 2015, a total of 182 patients were selected as study subjects from newly diagnosed RE patients.The anxiety and depression scores were evaluated according to Hamilton anxiety scale (HAMA) and Hamilton depression scale (HAMD).And then patients were divided into HAMA and HAMD negative conventional treatment group and combined treatment group, HAMA and HAMD positive conventional treatment group and combined treatment group.Rabeprazole and mosapride were administrated in conventional treatment group.For patients in combined treatment group, on the base of conventional treatment flupentixol and melitracen were added.The treatment course was eight weeks.The degree of anxiety and depression, RE symptoms and mucosal healing under gastroscope were evaluated before and after treatment.Adverse drug reaction was observed.Chi square test or t test was performed for statistical analysis.Results Eight weeks after treatment, the scores of HAMA and HAMD in HAMA and HAMD positive combined treatment group were 7.930 ±3.832 and 9.630 ± 3.650, which were both lower than those of conventional treatment group (11.660 ± 4.108 and 12.170 ± 4.459), and the differences were statistically significant (t=3.683 and 2.233;both P＜0.05).The symptom scores of heartburn, regurgitation, chest pain of HAMA and HAMD positive combined treatment group were 0.700±0.591,0.780± 0.629 and 0.720±0.621, respectively, which were lower than those of conventional treatment group (1.280 ± 0.502, 1.370 ± 0.610 and 1.040 ± 0.842), and the differences were statistically significant (t =5.133, 4.413 and 2.114, all P＜0.05).There were no statistical significance in symptoms scores between HAMA and HAMD negative combined treatment group and conventional treatment group (all P＞0.05).After treatment, the mucosal healing rate of HAMA and HAMD positive combined treatment group was 91.3％ (42/46), which was higher than that of conventional treatment group (71.7 ％, 33/46), and the difference was statistically significant (x2 =5.845, P =0.016).The incidence of adverse events of HAMA and HAMD negative combined treatment group was 4.8％ (2/42), and that of HAMA and HAMD positive combined treatment group was 2.2％(1/46).Conclusions The conventional treatment combined with flupentixol and melitracen in RE patients accompanied with anxiety and depression was remarkable and safe.RE patients without obvious anxiety or depression, preventive use of antianxiety and antidepressant medicine can not improve the efficacy.

Objective:To isolate rabbit fibroblast-like synoviocytes(FLSs) by suspended explant culture method. Methods:The healthy rabbit joint synovial layers were obtained. The cells were cultured by suspended explant culture method and compared with the explants adherent culture method. The growth status and morphology were observed. The growth curve of the 3rd passage cells was measured by CCK-8 assay,and the expression of vimentin was tested by immunocytochemistry. Results: The FLS obtained by the two methods exhibited a spindle-shaped appearance and could rapidly expand. The cell growth curve was typical of S type, and the cells highly expressed vimentin. Conclusion:Primary culture of rabbit fibroblast-like synoviocytes by suspended explant culture method were successfully established. The method was simple and highly efficient. It provided a new method for the isolation of FLS in vitro.

Objective A foundation for one of the biological components,Spirulina,to be applied in Controlled Ecological Life Support System(CELSS)would be laid with exploring effects of ionization radiation on the growth of Spirulina.Methods By using the ?-rays of ~(60)Co,Spirulina were irradiated.The dose of the ionization radiation covered 0,0.5,1.0,1.5,2.0,2.5,3.0 kGy.After irradiated,these Spirulina were cultured under the same conditions.The growth state,shape change,photodensity change,photosynthetic efficiency(O2 produced),and trophic physiological indexes of Spirulina or its solution,were observed,measured and analyzed.Its anti-irradiation ability was investigated.Results After irradiared with ~(60)Co ray,the growth rate,photosynthetic O2 produced efficiency,length of fila and other trophic physiological indexes were all affected in certain degree,but as compared to the higher plants,Spirulina have stronger radiation proof and self-rehabilitation capacity.Even if under high radiation condition(3.0 kGy),there was no complete death of cells,and the dose resulted in 50% death of the Spirulina was 2.0 kGy.Conclusion Spirulina has stronger ionization radiation proof and self-rehabilitation capacity,it can be considered as one of the key biological components in CELSS for future long-term space missions.

Objective To study the cultivating technique of wheat under low pressures as to establish the basis of developing hypobaric controlled ecological life support system.Methods Wheat growing experiments under normal and low pressure(101 kPa,50 kPa) were carried out with low pressure plant-cultivating facilities,and the growing indexes of wheat under low pressure,such as photosynthetic rate,transpiration rate,growing status,yield and nutrient contents in seeds were compared with those under normal pressure.Results Wheat grew normally under low pressure as compared with those grown under normal pressure,but low pressure curled leaves of the wheat and caused them to turn yellow at prophase;after 15 d treatment under hypobaric condition,the average rates of photosynthesis and transpiration increased by 9.23%and 11.54% respectively;shoot height of wheat decreased,both tiller number of each plant and the ratio of root in whole plant increased,but harvest time was delayed for about 5 d under low pressure;the biomass and the yield of wheat increased by 5.46% and 4.39% respectively,the contents of protein and total saccharide in seeds also enhanced at reduced pressure,but other nutrient contents in seeds had no significant difference between 101 kPa and 50 kPa.Conclusion Growth of wheat is promoted in certain degree by low atmospheric pressure.

Objective To develop a prototype of low pressure plant-cultivating facility, for studying the growing technique of higher plants under low pressure in manned deep space exploration, so as to lay foundations for establishing controlled ecological life support system (CELSS) in low pressure environment. Methods According to detailed demonstration of technical plan, blueprint design and machining of components, facility installment, debugging, trial operations and verification experiments were carried out. Results Two sets of low pressure plant-cultivating facilities were manufactured, both of them could be used to cultivate higher plant under low or normal pressure. They worked stably and their technical parameters such as temperature, relative humidity, air pressure, O2 partial pressure, CO2 partial pressure and water content of matrix were all monitored and controlled effectively. Conclusion Their main technical indexes meet design and experimental requirements, low pressure plant-cultivating experiments can be carried out with the facilities effectively.

Objective To investigate the characteristic of growth and development of vegetable at hypobaric environment and offer the cultivating technique of controlled ecological life support system for higher plant.Methods The means of comparative measurement was adopted to study the growth and physiological characteristic of lettuce under low and normal pressure(40.0 kPa,101.0 kPa),which was carried out with the low pressure plant-cultivating facility.Results Compared with normal pressure,germination rate of lettuce seed was eliminated by 7.62% under total pressure 40.0 kPa and oxygen partial pressure 8.4 kPa conditions;harvest time of lettuce was delayed,so biomass harvested at the same time reduced by 13.67% and content of total saccharide declined;the average rates of photosynthesis and transpiration were lower than those at normal pressure in 30 days after planting(DAP),but which reversed after that;release of C2H4 and shoot height reduced by 38.79% and 11.68%,but leaf area,ratio of root to total biomass and root vigor increased by 15.43%,2.57% and 40.53%,respectively.Conclusion Seed germination,growth and development of lettuce are affected in some degree at total pressure 40.0 kPa and oxygen partial pressure 8.4 kPa,some measures should be adopted to regulate the growth of lettuce.

Objective To investigate growing characteristics of plants grown at elevated CO2 concentrations in controlled environment and provide base for determination of biological components in future space controlled ecological life support system (CELSS).Methods Lettuce (Lactuca sativa var.Youmaicai) was cultivated at higher atmospheric CO2 concentration gradient (0.05%～2.0%).Its growing status,physiological characteristics and the content of nutrients were evaluated.Results There were some active effects of elevated CO2 (0.1% to 1.0%) on the average rates of photosynthesis and transpiration of the collective lettuce leaves and the contents of chlorophyll and carotenoid,but excessive CO2 (1.5% and 2.0%) weakened these effects,and even had some negative impacts.Lettuce could grow normally at elevated CO2 (0.1% to 2.0%),average shoot height and leaf area of lettuce plants treated at 0.1%～2.0% CO2 all increased,but the numbers of leaves eliminated gradually.The contents of nitrogen,potassium and Vc in plants decreased,phosphorus content rose and microelement contents had no obvious change in various CO2 treatments.Conclusion The lettuce can grow nearly normally at higher atmospheric CO2 concentration without serious growing inhibition or death,which indicated that the Youmaicai held stronger ability of tolerating higher concentration of CO2,so it's suitable to be determined as a candidate biological component in CELSS.

Objective To investigate the characteristics of higher plant grown in red and/or blue light and test the capability of red and/or blue light emitting diodes(LEDs) used as the artificial lights for plant growth in controlled ecological life support system(CELSS).Methods Lettuce(Lactuca sativa var.Youmaicai) was cultivated in sole white LED,sole red LED and combined red and blue LEDs,respectively.Its growing status,physiological characteristics and the content of nutrients were evaluated,which were adopted to select the best combination of red and blue LED for plant growth.Results Red light promoted the radial growth of lettuce plants,and blue light was propitious to their transverse extension and synthesis of chlorophyll and carotenoid;lettuce plants couldn't grow normally in sole red light;with the gradual increase of blue light proportion(5%~20%),there were some active effects on the growth of lettuce plants,for instance,the improvement of photosynthesis,transpiration and fluorescence(Fo,Fv/Fm and qN),the increasing of contents of chlorophyll,carotenoid and nutrients and the enhancement of total biomass;the adverse effects of blue LED on the growing of lettuce plants appeared gradually with the addition of blue light(25%~50%).Its biomass of shoot decreased significantly,which induced the increase of the ratio of root/shoot.Conclusion The combined red and blue LEDs is propitious to lettuce(var.Youmaicai) growth and the best lighting condition is the combined 90% red and 10% blue light.

To explore the feasibility of the application of LD 1 laser dust monitor to determination of inhalable particulates(IP) in indoor air of public places, and provide basis for establishment of national standard method of detection of IP in indoor air of public places LD 1 laser dust monitor and weighing filter paper method were applied in monitoring the concentration of IP in indoor air of 4 kinds of public places in Changzhou in four seasons simultaneously Significant correlation was observed between mass concentration and relative concentration ( r =0 7526, P