Objective:To compare liver adhesive suspension method and V-shaped liver suspension of anterior abdominal wall in laparoscopic radical gastrectomy.Methods:This study retrospectively evaluated 115 patients who underwent laparoscopic radical gastrectomy from Jan 2018 to Dec 2019 at Shanxi Provincial People′s Hospital, 59 cases were under liver adhesive suspension (study group) and 56 cases under V-shaped liver suspension of anterior abdominal wall (control group). Intraoperative and postoperative conditions were observed and compared between the two groups.Results:All the 115 cases successfully completed surgery. The liver suspension time in study group was (2.4±0.5)min, which was significantly shorter than that of the control group((12.5±2.6) min)( t=-28.100, P<0.01). The total operation time in was (284±69) min and (307±78) min, respectively, ( t=-1.656, P=0.10). There were no statistically significant differences between the two groups in preoperative ALT, AST, total bilirubin, intraoperative incidence of hepatic lacerations and subcapsular hematoma, postoperative total bilirubin level, AST on the day 7 postoperatively, tumor location, TNM staging, surgical method, postoperative duration of hospital stay, and VAS score ( P>0.05). Conclusions:Both methods of liver suspension are safe and feasible in laparoscopic surgery for gastric cancer.Compared with V-shaped liver suspension of anterior abdominal wall, liver adhesive suspension has the advantages of less invasive, safe, and time-saving.

A series of novel 4-substituted-3-nitrobenzamide derivatives were designed and synthesized. The structures of the target compounds were confirmed with 1H NMR, 13C NMR, MS and element analysis. Anti-tumor activities against HCT-116, MDA-MB435 and HL-60 cell lines in vitro were evaluated by SRB assay. The results indicated most of the target compounds exhibited potent anti-tumor activity. Compound 4a showed the most potent inhibitory activities against three cancer cell lines with the GI50 values of 1.904-2.111 micromol x L(-1). Compounds 4g, 41-4n exhibited more potent inhibitory activities against MDA-MB435 and HL-60 cell lines with the GI50 values of 1.008-3.586 micromol x L(-1) and 1.993-3.778 micromol x L(-1), respectively. The structure-activity relationship of these compounds is discussed preliminarily.

Background and purpose: MicroRNAs (miRNAs) play an important role in tumor biological behavior. miRNAs are down-regulated or up-regulated in various cancer types, triggering abnormal cell differentiation, proliferation and apoptosis. This study was designed to investigate the expression and clinical signiifcance of miR-187*in colorectal cancer (CRC), and further to investigate its roles in promoting cell apoptosis. Methods:The expressions of miR-187* in 40 CRC cases were examined by real-time quantitative reverse transcription-PCR (qRT-PCR). The relationship between miR-187*expression and clinical features of CRC was analyzed. HCT116 cells were transfected with a miR-187*mimic and the apoptosis of the transfected cells were examined by lfow cytometry (FCM). Results:The expression of miR-187*was down-regulated in CRC tissues 0.165 (0.106, 0.428) compared with those in normal tissues 0.334 (0.211, 0.712) (P<0.05), especially in mucinous carcinoma and older age CRC (P<0.05). Transfection of HCT116 cells with a miR-187*mimic up-regulated the expression of miR-187*and increased cell early apoptosis (P<0.05). Conclusion: The expression level of miR-187* was lower in CRC. miR-187* expression correlates with histological type and age. Transfection of HCT116 cells with a miR-187*mimic accelerates apoptosis of tumor cells, suggesting that miR-187*is a potent tumor suppressor.

A series of [1,3]dioxolo[4,5-f]isoindolone derivatives were designed, synthesized and evaluated as inhibitors of acetylcholinesterases (AChE). Furthermore, their effects on memory impairment of mice induced by scopolamine were investigated with step-through test. The results suggested that most of the target compounds exhibited potential inhibition on AChE with IC50 values at micromolar range. Compounds I1 (IC50 value of 0.086 μmol · L(-1)) and I2 (IC50 value of 0.080 μmol · L(-1)) showed the strongest AChE inhibitory activity, which are equipotent to donepezil (IC50 value of 0.094 μmol · L(-1)). Moreover, compounds I1-I4 could improve the memory impairment induced by scopolamine in mice.

Objective To explore the status of job burnout of medical staffs in minority border areas. Methods A total of 906 medical personnel from Pu'er hospitals in Yunnan Province were as-sessed with Maslach Burnout Inventory General Survey (MBI-GS) by t test, descriptive statistics, chi-square test, ANOVA and LSD-t pairwise comparison. Results ①The emotional exhaustion, cynicism and reduced personal accomplishment of medical staffs in minority border areas (1.55±1.40, 0.99± 1.29, 2.47±1.98) are significantly lower than general areas (P=0.000, 0.010, 0.000); ②emotional ex-haustion, cynicism and Maslach Burnout Inventory (MBI) total points of different levels of hospital medi-cal staff, the score of which in descending order are:municipal, county and township (town) levels (P=0.000, 0.000, 0.000); ③Female medical personnel's emotional exhaustion and cynicism are scored significantly higher than males medical staff (P=0.000, 0.025); ④The scores of emotional exhaustion, cynicism, reduced personal accomplishment and MBI total points of Bachelor medical staff are signifi-cantly higher than those of colleges as well as secondary schools and below (P=0.000, 0.000, 0.000, 0.012); ⑤The scores of reduced personal achievement of Lahu medical staff are higher than those of other ethnic groups (P=0.009). Conclusion In medical staffs in minority border areas there exists a certain extent of job burnout, which presents differences among genders, educational background, hos-pitals and ethnic groups.

Objective To investigate the expression and clinical significance of microRNA-224 and microRNA-378e in colorectal cancer tissues and normal mucosa adjacent to tumor lesions. Methods The gene chip technology was used to detect the different expression of miRNA in colorectal carcinoma tissues and adjacent normal tissues, which was then confirmed by real-time PCR. The relationship between the pathology and clinical data was analyzed. Results The expres-sion level of miR-224 was significantly up-regulated in tumor tissue, while miR-378e was down-regulated in tumor tissue, which was confirmed by real-time PCR. The expression of miR-224 was strongly associated with histological types, while miR-378e was strongly associated with the infiltration depth of colorectal cancer. Conclusion miR-224 is a potent tumor promoter, while miR-378e is a potent tumor suppressor. Both miR-224 and miR-378e can be used as potential colorectal cancer molecular markers.

Objective To investigate the expression of 8-hydroxy deoxy guanosine(8-OHdG)and nectin-4 in the serum of primary liver cancer(PLC),and to evaluate the efficacy of transcatheter chemoembolization(TACE)for PLC.Methods From January 2021 to June 2022,180 patients with primary liver cancer with TACE were studied.According to the efficacy of TACE patients,they were separated into a good group(n=137)and an adverse group(n=43).The general clinical data and the serum expression levels of 8-OHdG and nectin-4 were compared between the two groups;multivariate Logistic regression was applied to analyze the influencing factors of postoperative efficacy in TACE for primary liver cancer;receiver operating characteristic was applied to analyze the value of serum 8-OHdG and nectin-4 levels in evaluating the efficacy of TACE for primary liver cancer.Results There were no significant differences in age,sex,BMI,Child-Pugh grade,tumor location,tumor number,tumor diameter,tumor contour,degree of differentiation,tumor envelope,vascular cancer thrombus,bile duct cancer thrombus and lymph node metastasis between the poor postoperative efficacy group and the good efficacy group after TACE for primary liver cancer(P＞0.05).The expression levels of serum 8-OHdG and nectin-4 in the poor postoperative efficacy group after TACE for primary liver cancer were obviously higher than those in the good efficacy group(P＜0.05).Multivariate Logistic regression analysis showed that serum 8-OHdG,nectin-4,tumor diameter,TNM staging and Lymph node metastasis were all independent influencing factors for the postoperative efficacy of TACE in primary liver cancer(P＜0.05).The AUC of the combined evaluation of serum 8-OHdG and nectin-4 for the postoperative efficacy of TACE in primary liver cancer was 0.930,with a sensitivity of 86.05％and a specificity of 94.16％,which was superior to their respective individual evaluations(Zcombination-8-OHdG=2.033,Zcombination-nectin-4=3.221,P=0.042,0.001).Conclusion The serum levels of 8-OHdG and nectin-4 are obviously increased in the poor postoperative efficacy group after TACE for primary liver cancer.The combination of the two has a good evaluation effect on the postoperative efficacy of TACE for primary liver cancer.

Objective To analyze the 14 indicators of on-site evaluation at 18 hospitals under Beijing hospital authority in 2016 , and to provide technical support and reference for further optimization of medical quality and service .Methods According to the "Beijing Municipal Administration of Hospital 2016 On-site Evaluation Indicators", the results were analyzed using the fuzzy combined method of TOPSIS and rank sum ratio , and the ranking of the indicators was sorted .Results TOPSIS method and rank sum ratio weighted fuzzy joint analysis showed that the top three indicators from high to low were medical technology management , patient identification and verification system , clinical care service management;and the last three indicators were hospital-acquired infection monitoring , medical malpractice reporting and management , critical value report and disposal .Conclusions Hospitals are recommended to strengthen their exchange , popularize the concept of continuous improvement , the use of management tools to solve practical problems , and further improve the hospital medical quality and service quality .

Objective To investigate the epidemiologic characteristics and risk factors for congenital hypothyroidism (CH) in Beijing between the years 1989 and 2014.Methods Information on neonatal screening,and diagnoses and treatment of CH from 1989 to 2014 were obtained from the database of the Beijing Neonatal Screening Center.The screening parameter was thyroid-stimulating hormone (thyrotropin;TSH),which was measured by radioimmunoassay (RIA) from 1989 to 1995,enzyme-linked immunosorbent assay (ELISA) from 1996 to 2003,and time-resolved fluorescence immunoassay (DELFIA(R))from 2004 to 2014.The cutoff value of each screening method was set as the international standard for the corresponding years (20 mIU/L from 1989 to 1995 and 10 mIU/L from 1996 to 2014).CH was diagnosed using "The Technical Specification of Diagnosis and Treatment of Phenylketonuria and Congenital Hypothyroidism",published in 2010 by the Ministry of Health of the People's Republic of China.Data on live birth infants were obtained from Beijing obstetric quality reports.The incidence of CH using different screening methods was compared,and trends in annual incidence were analyzed.To exclude the influence of different screening methods,data from the years 2004 to 2014 were used to identify the risk factors for CH.Results Between 1989 and 2014,the incidence of CH in Beijing was 36.7 per 100 000 individuals,with permanent CH (PCH) and transient CH (TCH) having incidences of 16.4 per 100 000 and 15.9 per 100 000,respectively.The annual incidence of CH increased from 11.2 per 100 000 in 1989 to 51.0 per 100 000 in 2014 (x2119.02,P＜0.001),with PCH increasing from 5.6 to 16.0 per 100 000 (x2=34.38,P＜0.001) and TCH increasing from 5.6 to 13.0 per 100 000 (x2=26.93,P＜0.001).Among the PCH cases,70.44％ (255/362) were thyroid dysgenesis or ectopic glands,while the other 29.56％ (107/362) were dyshormonogenesis.Between 2004 and 2014,the incidence of CH in females (51.7/100 000) was higher than in males (37.0/100 000),and it was higher in post-term (334.5/100 000) and preterm births (77.8/100 000) than that in term births (41.4/100 000).It was also higher in the low birth weight (87.7/100 000) than the normal (42.4/100 000)and high birth weight (42.6/100 000) populations.Conclusions Between 1989 and 2014,there was a tendency towards an increase in the overall incidence of CH,and the incidence of both PCH and TCH in Beijing.Female sex,preterm birth,older gestational age,low birth weight,and preterm birth were risk factors affecting the incidence of CH in Beijing.

Objective To investigate the epidemiologic characteristics and risk factors for congenital hypothyroidism (CH) in Beijing between the years 1989 and 2014.Methods Information on neonatal screening,and diagnoses and treatment of CH from 1989 to 2014 were obtained from the database of the Beijing Neonatal Screening Center.The screening parameter was thyroid-stimulating hormone (thyrotropin;TSH),which was measured by radioimmunoassay (RIA) from 1989 to 1995,enzyme-linked immunosorbent assay (ELISA) from 1996 to 2003,and time-resolved fluorescence immunoassay (DELFIA(R))from 2004 to 2014.The cutoff value of each screening method was set as the international standard for the corresponding years (20 mIU/L from 1989 to 1995 and 10 mIU/L from 1996 to 2014).CH was diagnosed using "The Technical Specification of Diagnosis and Treatment of Phenylketonuria and Congenital Hypothyroidism",published in 2010 by the Ministry of Health of the People's Republic of China.Data on live birth infants were obtained from Beijing obstetric quality reports.The incidence of CH using different screening methods was compared,and trends in annual incidence were analyzed.To exclude the influence of different screening methods,data from the years 2004 to 2014 were used to identify the risk factors for CH.Results Between 1989 and 2014,the incidence of CH in Beijing was 36.7 per 100 000 individuals,with permanent CH (PCH) and transient CH (TCH) having incidences of 16.4 per 100 000 and 15.9 per 100 000,respectively.The annual incidence of CH increased from 11.2 per 100 000 in 1989 to 51.0 per 100 000 in 2014 (x2119.02,P＜0.001),with PCH increasing from 5.6 to 16.0 per 100 000 (x2=34.38,P＜0.001) and TCH increasing from 5.6 to 13.0 per 100 000 (x2=26.93,P＜0.001).Among the PCH cases,70.44％ (255/362) were thyroid dysgenesis or ectopic glands,while the other 29.56％ (107/362) were dyshormonogenesis.Between 2004 and 2014,the incidence of CH in females (51.7/100 000) was higher than in males (37.0/100 000),and it was higher in post-term (334.5/100 000) and preterm births (77.8/100 000) than that in term births (41.4/100 000).It was also higher in the low birth weight (87.7/100 000) than the normal (42.4/100 000)and high birth weight (42.6/100 000) populations.Conclusions Between 1989 and 2014,there was a tendency towards an increase in the overall incidence of CH,and the incidence of both PCH and TCH in Beijing.Female sex,preterm birth,older gestational age,low birth weight,and preterm birth were risk factors affecting the incidence of CH in Beijing.