2.Analysis on staphylococcus xylosus inducing postoperative nosocomial infection
Bangxing HONG ; Lifang JIANG ; Shiying ZHANG ; Min ZENG
Chinese Journal of Disease Control & Prevention 2001;5(2):114-115
Objective To investigate the pathogens inducing a posto perative nosocomial infection. Methods Specimens was collected fro m exudates or air for bacteria culture and identification. Re sults The postoperative infection was induced by staphylococcus x ylosus. Conclusions The relevant factors affecting the po stoperative nosocomial infection include incomplete sterilization of operative r oom and operative tools. Thus strict control measures must be put into effect.
3.Psychological experience of adolescent malignant lymphoma patients: a qualitative research
Ruiqing CAI ; Lifang LIANG ; Meixia ZHONG ; Yulian ZENG
Chinese Journal of Practical Nursing 2015;31(z1):160-162
Objective This research aims to provide a theoretical basis for the nursing care for adolescent malignant lymphoma patients by probing into their emotional experience.Methods In-depth interview of qualitative study is adopted in the interviews with 9 adolescent malignant lymphoma patients,while Colaizzi' s seven-step data analytical approach is utilized to analyze and sort the data before the main idea is concluded and refined.Results These adolescent malignant lymphoma patients,with complicated psychological changes and a suicidal tendency,find it hard to cope with stress.Conclusions Nursing care should be provided to such patients and there should be a respect for their rights to know their disease as well as a timely crisis intervention to those who have a suicidal tendency.Furthermore,due attention should be given to the mental states of the family members and the stability of the family of the patients.We also call for the love and more support for these patients from the government and caring people in society.
4.Clinical studies of hs-CRP,Hcy,D-D,Fib,SF joint detection to predict progressive cerebral infarction
Aili LI ; Lifang HUANG ; Zhiying BAO ; Jianbing ZENG
International Journal of Laboratory Medicine 2015;(15):2202-2203
Objective To discuss the value of joint detection in patients with progressive cerebral infarction (PCI) on high sensi‐tivity c‐reactive protein(hs‐CRP) ,and homocysteine(Hcy) ,D‐Dimer ,Fibrinogen(Fib) ,ferritin(SF) .Methods According to the de‐velopment and evolution of the 156 patients with acute cerebral infarction were divided into progressive cerebral infarction group and non‐progressive cerebral infarction group ,to determine the level of serum hs‐CRP ,Hcy ,one or two D‐Dimer ,Fib ,SF .Results hs‐CRP ,Hcy ,D‐D ,Fib ,SF in the PCI group were higher than those in the control group ,the difference was statistically significant (P<0 .05) .Conclusion Between the development of the PCI with hs‐CRP ,Hcy ,D‐D ,Fib and SF ,there is a close relationship ,to detect the level has a certain significance to the prediction of progressive ischemic stroke .
5.Analysis of perception control and influencing factors in patients with lung cancer undergoing chemotherapy
Xing TAN ; Lifang HUANG ; Caixia SUN ; Rui JIANG ; Guqing ZENG
Chinese Journal of Practical Nursing 2016;32(29):2258-2262
Objective To analyze the perceived control of lung cancer patients undergoing chemotherapy and to explore its influencing factors. Methods A total of 185 patients with lung cancer were recruited and assessed by homemade general information questionnaire and the Cancer Experience and Efficacy Scale. Results The total score of cancer experience during chemotherapy in patients with lung cancer was (62.50±6.99) points. The total score of efficacy during chemotherapy in patients with lung cancer was (42.52±8.08) points. Single factor analysis showed the influencing factors of perceived control were personal income, sex, education, metastasis, surgical treatment and stage of disease. Conclusions The cancer-related experience and efficacy of patients with lung cancer is at middle level. Health-care worker should pay attention to the impact of lung cancer patients and take effective measures to mitigate the negative cancer-related experience, thereby improving the perceived control of the lung cancer patients.
6.Application of modified three-step fluorescence in situ hybridization in prenatal diagnosis
Yan ZENG ; Jiaming FAN ; Lifang ZHANG ; Feiyan QIAN ; Ping XU ; Weijun JIANG
Chinese Journal of Laboratory Medicine 2014;37(4):266-269
Objective To investigate a simple and efficient fluorescence in situ hybridization method for prenatal diagnosis.Methods Thirty-six cases of chorionic villus samples were hybridized with probes 18/X/Y,13/21 by using traditional cuhure methods and modified three-step methods in controlled experimentation during 2012 to 2013.The hybridization rate and fluorescence signals were analyzed.Results A total of 72 hybrid zone were detected.Probe 21/13 hybridization rate and fluorescence signals of three-step modified methods were higher than that of traditional methods(99.72% ±0.42% vs 85.90% ±4.15%,t =20.4,P <0.01; 2.58 ±0.50 vs 1.52 ± 0.55,t =7.53,P <0.01).Probe 18/X/Y has the same hybridization rate and fluorescence signals between three-step modified methods and traditional methods (99.57% ±0.53% vs99.70% ±0.42%,t=1.30,P>0.05; 2.22±0.42 vs2.36±0.48,t=1.57,P>0.05).The coincidence rate of two methods was 100% (36/36).Conclusion The modified fluorescence in situ hybridization three-step methods in the study was simple,rapid,effective and environment-friendly.At the same time,it has some defects such as the signals of 18/X/Y was not concentrated enough.Further exploration is needed.
7.The expression and significance of nephrin in hepatitis B virus-associated membranous nephropathy
Yongze ZHUANG ; Lifang WEI ; Yinghao YU ; Ling ZENG ; Xisheng XIONG ; Zaizeng WU
Chinese Journal of Internal Medicine 2011;50(9):766-770
ObjectiveTo observe the expression of nephrin in hepatitis B virus-associated membranous nephropathy (HBV-MN),and investigate the impairment and significance of podocyte in HBV-MN.MethodsThe protein expression of nephrin in renal biopsy specimens in 35 patients,who were diagnosed as HBV-MN by renal biopsy,was determined by immunohistochemistry and tested by semiquantitative method.The relationship between the expression of nephrin and clinicopathological data was analyzed.Results Among the 35 cases with HBV-MN,6 were in MN phase Ⅰ,20 in MN phase Ⅱ and 9 in MN phase Ⅲ.A strong intensity expression of nephrin in normal glomerulus was found along capillary loop of glomerulus,while its expression in HBV-MN patients decreased obviously.There was no significantly difference in the expression of nephrin among the different stages of HBV-MN ( P > 0.05 ).The expression of nephrin in different clinical types was significantly different ( P < 0.05 ).The expression of nephrin in patients with nephrotic syndrome was significantly lower than that in patients without nephrotic syndrome (P <0.01 ).The expression of nephrin in different grades of 24-hour urinary protein excretion quantity was significantly different( P < 0.05 ).There was negative correlation between the expression of nephrin and 24-hour urinary protein excretion quantity(r = -0.378,P < 0.05).In the patients with HBV-MN phase Ⅱ,the expression of nephrin in patients with nephrotic syndrome was also significantly lower than that in patients without nephrotic syndrome( P < 0.01 ).Conclusions The damage of podocytes emerge in the early stage of HBV-MN and the expression of nephrin in HBV-MN patients,especially in patients with nephrotic syndrome,are significantly down regulated.The descended expression of nephrin in HBV-MN patients may promote the production of proteinuria.
8.Simple and sensitive determination of sparfloxacin in pharmaceuticals and biological samples by immunoassay
Huajin ZENG ; Ran YANG ; Bing LIU ; Lifang LEI ; Jianjun LI ; Lingbo QU
Journal of Pharmaceutical Analysis 2012;02(3):214-219
Plasma quinolone concentrations are not routinely measured in clinical practice.However,in order to optimize quinolone treatment,monitoring of plasma concentrations could sometimes be useful particularly in critically ill patients.In this study,anti-sparfloxacin antibody was obtained by immunizing rabbits with sparfloxacin conjugated with bovine serum albumin using isobutyl chloroformate method.After the assay procedure was optimized,the standard curve of sparfloxacin was established.The practical measuring range of the competitive ELISA extended from 5 ng/mL to 2 μtg/mL.The recovery rates and coefficients of variation for rat plasma,urine and tissues were 87.7-106.2% and 4.8-15.3%,respectively.To demonstrate the potential of the ELISA,a preliminary pharmacokinetics and tissue distribution study of sparfloxacin in rats and quantitative analysis of sparfloxacin in several pharmaceuticals were performed and compared with high-performance liquid chromatography (HPLC).The experimental data indicated that the proposed method would be a valuable tool in therapeutic drug monitoring (TDM) for sparfloxacin.
9.Effect of Scalp Acupuncture on Cognitive and Motor Functions of Stroke Patients in Recovery Stage
Dongling XIE ; Lifang ZHU ; Huiyu LIU ; Junbin CHEN ; Chunying ZENG ; Suxia WANG ; Jin LI
Chinese Journal of Rehabilitation Theory and Practice 2007;13(6):542-543
Objective To observe the effect of scalp acupuncture on cognitive and motor functions of stroke patients in recovery stage.Methods80 stroke patients with cognitive disorder were randomly divided into the scalp acupuncture group (n=41) and control group (n=39). All patients of two groups were treated with routine therapy and rehabilitation training, but the cases of the scalp acupuncture group were added with scalp acupuncture. The cognitive function, motor function and activities of daily living (ADL) of all patients were assessed by delitescence and amplitude of event-related potential (ERP) P300, Fugl-Meyer Assessment (FMA), Barthel Index (BI) respectively before and 3 months after treatment.ResultsAfter treatment, P300 delitescence of the patients in the scalp acupuncture group shorten to 38 ms in average while in the control group shorten only to 17 ms, the therapeutic effect of the scalp acupuncture group was superior to that of the control group ( P<0.05). The scores of FMA and BI of the patients in the scalp acupuncture group were also better than those in the control group ( P<0.05).ConclusionScalp acupuncture can efficiently improve the cognitive function of stroke patients in the recovery stage, and promote the recovery of motor function and ADL.
10.Expression and pathological features of CXCL13 in clinical serum in patients with primary hepatic carcinoma
Hao ZENG ; Ying HUI ; Zhidong WU ; Lifang HUANG
International Journal of Laboratory Medicine 2018;39(13):1557-1561
Objective To evaluate the expression and role of serum CXC chemokine ligand 13 (CXCL13) in Primary hepatic carcinoma (PHC) patients and explore the clinical value of PHC diagnosis and prognosis . Methods Serum samples were collected from 80 patients with PHC ,80 patients with chronic hepatitis B (CHB) and 36 healthy controls (HC) .Serum levels of CXCL13 in patients were measured by enzyme-linked immunosorbent assay (ELISA) .The relationship between clinicopathological features and laboratory parame-ters was analyzed by statistical software .The correlation between CXCL13 level and prognosis of liver func-tion were tested by spearman correlation analysis ,the diagnostic value of CXCL13 and AFP to PHC were ana-lysed by ROC curve .Results The levels of serum CXCL13 in patients with PHC were significantly higher than those in CHB and HC groups .The levels of serum CXCL13 in patients with advanced PHC (Ⅲ - Ⅳ) were significantly higher than those in patients with early PHC (Ⅰ - Ⅱ) .Serum levels of CXCL13 in patients with tumor diameter more than 5cm were significantly higher than those tumor diameter less than or equal 5 cm .Patients that metastatic serum levels of CXCL13 were significantly higher than without tumor metastasis in patients .The level of serum CXCL13 in patients with ascites was significantly higher than that in patients without ascites ,all the data were statistically significant (P<0 .05) .Serum levels of CXCL13 in patients with PHC were correlated with hemoglobin ,serum albumin ,cholinesterase ,and international normalized ratios . There was a positive correlation between serum CXCL13 concentration and Child-Pugh score in PHC patients (r= 0 .459 ,P= 0 .001) ,and negatively correlated with serum albumin and cholinesterase (r= -0 .319 ,-0 .259 ,P=0 .004 ,0 .008) .CXOC13 and AFP combination of the ROC curve were 0 .938 .Sensitivity and spe-cificity were 82 .8% and 100 .0% .Conclusion High expression of serum CXCL13 in PHC is closely related to tumor grow th and metastasis ,and has important clinical value in the diagnosis ,treatment and prognosis evalu-ation .