Objective To investigate the effect of intravenous immunoglobulins G on rat subjected to chronic un-predictable stress. Method Rats were randomly divided into 2 groups (IVIG+stress group and stress-only group). Sucrose preference test, force swim test and neurogenesis analyze were used to analyze the effect of IVIG on rats in stress group. Results Sucrose consumption in IVIG group was significantly more than CUS group(P<0.01), while non floating time of force swim was significantly lower than CUS group(P<0.01). The difference of BrdU positive cell number in Hippocampus between two groups was significant(P<0.01). Conclusion IVIG show an antidepressant like effect, the mechanism may be involved in eliminate inflammatory cytokine of peripheral circulation.

Objective To investigate the positive rates of HBsAg and abnormal rates of Alanine aminotransferase among high school graduates in Jingjiang city.Methods HBsAg were detected by ELISA and ALT was detected by rate assay among 20 988 high school graduates during 2004—2007 in Jingjiang city.Results The HBsAg positive students were 1754.The total positive rate of HBsAg was 8.36%.The positive rate of HBsAg of male(9.46%)was higher than that of female(6.95%).The HBsAg positive rate of rural students(9.44%)was higher than that of urban students(7.28%).HBsAg positive rates of urban students showed a decreasing trend during 2004—2007,the rates were 9.31%,8.08%,6.74% and 5.24%,respectively(P0.05).There were 155 cases(8.4%)with increased ALT among 1754 HBsAg positive students.Conclusion The total positive rate of HBsAg among high school graduates in Jingjiang city from 2004—2007 was 8.36%.The HBsAg positive rate of male students was higher than that of female.The HBsAg positive rate of rural students was higher than that of urban students.8.4% students were found ALT abnormal.We should intensify Hepatitis B vaccine in time to prevent the spreading of HBV.

Objective To study the feasibility of using chitosan membrane to carry and transport melanocytes, in order to refine the technique for melanocyte transplantation with chitosan membrane. Methods Melanocytes were inoculated onto chitosan membrane and cultured for a period of time, then, electron microscopy,MTT assay and NaOH assay were carried out to estimate the adherence, growth and melanogenesis of the melanocytes. Skin wound surface was prepared in 12 nude mice, which were equally divided into 3 groups, test group inoculated with melanocytes on chitosan membrane, negative control group I treated with chitosan membrane without melanocytes, and negative control group II directly dressed immediately after the preparation of wound surface. On day 10 and 20 after the transplantation, confocal laser microscopy and immunohistochemistry were performed to observe the migration of melanocytes into the skin wound surface. Results Scanning electron microscopy and inverted microscopy showed that melanocytes were evenly distributed on and adhered well to the underlying chitosan membrane. As the growth curve of melanocytes demonstrated, chitosan membrane could support the normal growth of melanocytes, and no significant difference was observed in the synthesized melanin content between melanocytes cultured on the chitosan membrane and those in culture disks (0.087 ± 0.027 vs. 0.101 ± 0.036, t = 0.79, P > 0.05). Melanocytes were seen at the transplantation sites by confocal laser microscopy, and biopsy specimens from the transplantation sites stained positive for antimelan-A monoclonal antibody. Conclusions Melanocytes can adhere to and grow on the chitosan membrane,which can facilitate the migration of melanocytes to the transplantation sites in animals with the maintenance of biological activity of melanocytes.

Purpose:To evaluate the efficacy and toxicity of the low dose 5 Fluorouracil and Cisplatin continuous infusion for treatment of advanced gastric carcinoma. Methods:A total of 21 patients were enrolled in the trial, FP therapy consisted of 5 FU (0 25/m 2/d) every day by continuous infusion and cisplatin (6 mg/m 2/d) every day by continuous infusion for 3 weeks.Results:A complete response was observed in 2 cases and a partial response in 10 cases, for and overall response rate of 57.1%. Toxic effects included leukopenia, thrombocytopenia, nauesa and vomiting.Conclusions:FP therapy is a promising regimen for patients with advanced gastric carcinoma.

Objective:To provide chemical basis for processing of leech through investigating the variations of composition of leech scalded with talc powder. Methods: Amino acids were determined by HPLC post column derivatization and steroids by GC MS. Results: The results showed the total contents of amino acids in scalded leech were reduced and the steroids of it were changed. Conclusion: The processing method of leech used at the moment has a great influence in chemical components of leech.

Objective To explore the inner feelings of gestational diabetes at their primary use of the real-time blood glucose monitoring. Method Using the qualitative method, 10 gestational diabetic patients monitored their blood glucose using the real-time blood glucose monitoring for the first time and then were interviewed, the acquired results treated with phenomenological analysis. Result The inner feelings of the 10 patients were summarized including 5 themes:worry about the safety of the fetus, fear on alarming at monitoring, different views on four times input of finger blood glucose values, worry about inaccuracy in blood glucose monitoring data, changes in mental conditions and diets. Conclusion Health care workers should provide the gestational diabetic patients with targeted care strategies based on presence of psychological status and knowledge needs so as to improve their compliance, reduce the psychological burden of patients and control their blood glucose level.

Objective To analyze the clinical value of three-dimensional ultrasound in the diagnosis of pelvic floor dysfunction disease.Methods From June 2016 to December 2017,95 cases of female pelvic floor dysfunction disease in the People's Hospital of Dongyang were selected as observation group.And 90 healthy women received physical examination during the same period were selected as control group.Both two groups were subjected to three-dimensional ultrasonography,and the results were analyzed.Results In the observation group,the anteroposterior diameter and area in resting period,anteroposterior diameter and area in tension period,anteroposterior diameter and area in anus period of the anal pelvic diaphragmatic hiatus were (65.58 ± 6.38)cm,(20.01 ± 3.98) cm2,(68.40 ± 5.54) cm,(22.50 ± 3.12) cm2,(60.17 ± 5.55) cm and (19.28 ± 2.52) cm2,respectively,which were significantly higher than those in the control group [(51.84 ± 3.46) cm,(13.93 ± 2.09) cm2,(53.65 ± 4.23) cm,(14.60 ± 2.95) cm2,(48.95 ± 3.48) cm,(13.45 ± 1.76) cm2] (t =18.066,12.903,20.272,13.200,16.369,18.150,P =0.000,0.000,0.000,0.000,0.000,and 0.000).But the pubic rectal muscle thickness of resting period,tension period,anal contraction period in the observation group were (10.28 ± 1.96) cm,(10.63 ± 1.57) cm,and (10.01 ± 1.13) cm,respectively,which were lower than those in the control group [(10.28 ± 1.96)cm,(10.63 ±1.57)cm,(10.35 ±1.49)cm] (t =1.015,1.940,1.741,P =0.311,0.053,0.083).The observation group was asymmetrical,asymmetrical on both sides and interrupted continuously.In the control group,the echo was uniform,bilateral symmetry,without interruption.Conclusion The use of three-dimensional ultrasound in the diagnosis of female pelvic floor dysfunction disease has good clinical value.

ObjectiveTo establish an individualized culture system for melanocytes, and to estimate its efficacy for the treatment of large-area vitiligo. MethodsHu 16 medium was used for in vitro primary culture of melanocytes isolated from patients with stable segmental vitiligo.Doubling time(DOT), melanin content (M), melanin production(MP) and number of dendrites were examined to evaluate the biological activity of melanocytes. To obtain melanocytes with better biological activity, the components of Hu16 culture medium were adjusted. Ultra pulse CO2 laser was utilized to shave the vitiligous lesions and remove the epidermis followed by autologous transplantation. Follow-up was carried out. ResultsMelanocytes were obtained from 10 patients with stable segmental vitiligo and cultured. The melanocytes from 6 patients showed relatively short DOT, stable M and MP during the first and seventh passage, and were considered to be at initial or growth stage and applicable to transplantation. The remaining melanocytes from the other 4 patients had displayed long DOT, instable M, MP and dendrite quantity since the third passage; by adjusting the components of culture medium, these cells were induced into growth stage and finally applied to transplantation. A 12-month follow-up revealed that the repigmentation rate was higher than 90％ in 7 patients, ranged between 70％ and 80％ in the remaining 3 patients, with the transplantation area being 116.8 + 75.6 cm2. ConclusionsThe individualized culture system with adjusted components in culture medium yields melanocytes with satisfying biological activity, which are proved to be effective for the treatment of large-area, segmental and stable vitiligo.

Objective To evaluate the therapeutic effect of transplantation of autologous melanocytes cultured with individualized medium in vitiligo. Methods Donor skin was obtained by suction blisters from a normally pigmented area of the abdomen of 155 patients with vitiligo. The roof of the blisters was clipped and digested with trypsin, then the suspension of epidermal cells and melanocytes were cultured in Hu16 medium.The cell division time (DOT) and melanin content of cultured melanocytes were measured followed by the adjustment of concentration of fetal calf serum, cytokines and cAMP elevating agents based on the DOT,melanin content and morphology of melanocytes for the individualized culture of melanocytes. After 2 - 5 passages, melanocytes were harvested and inoculated into ultrapluse CO2 laser-denuded lesions. All patients were followed up for at least 6 months. Results One hundred and fifty-five vitiligo patients with 204 lesions were treated with transplantation of autologous melanocytes. Of the 155 patients, 119 received 1 session of transplantation, 36 received 2 to 4 session of transplantation. Cells were expanded by 50 - 80 times in vitro after individualized culture. Repigmentation was more than 50% in 84.8% of these lesions, more than 90% in 52.94% of the lesions. A homogeneous skin color was obtained in repigmented skin, and no scarring or other side effects were observed. No influence was noted on the outcome of transplantation for sex, age, course of disease or lesion size of patients. Segmental vitiligo showed better response than vitiligo vulgaris: the effective rate and cure rate were 93.62% and 65.96% respectively for segmental vitiligo, 82.16% and 49.04% respectively for vitiligo vulgaris. Lesions located on the arms and legs (not including elbows and knees) showed the best response, with a cure rate of 73.08%, whereas acral sites were the most difficult area to repigment, with a cure rate of just 25.93%. Conclusions Individualized culture can significantly increase the success rate of melanocyte culture and expanding times of melanocytes. Transplantation of cultured autologous melanocytes is an effective modality deserving clinical application in the treatment of stable vitiligo, with the advantage of treating large depigmented area with melanocytes from a small donor site.