Musashi is a family of RNA binding proteins with a conservative evolution. This protein family is selectively ex-pressed in the nervous system and comprises two members, namely, Musashi-1 and Musashi-2. Musashi-1 and Musashi-2 are transla-tional suppressors of Numb mRNA and can synergistically regulate the Notch signaling pathway;as a result, an asymmetric division of stem cells occurs. Musashi-1 is the first member of the family and was originally isolated from Drosophila. As a candidate stem gene, Musashi-1 participates in disease progression in stem cells. Musashi-1 is also an important protein that maintains the functions of stem cells, participates in tumor-related signaling pathways, and participates in cell proliferation and apoptosis. Furthermore, Musashi-1 is overexpressed in many solid tumors, such as neuroglioma, esophagus, gastric, colorectal, and breast cancers. Studies on Musashi-1 can provide new insights into genetic diagnosis and cancer treatments. In this study, the structure and function of Musashi-1 and the re-search progress of tumor mechanisms were summarized and reviewed.

Objective To investigate the effect of intravenous immunoglobulins G on rat subjected to chronic un-predictable stress. Method Rats were randomly divided into 2 groups (IVIG+stress group and stress-only group). Sucrose preference test, force swim test and neurogenesis analyze were used to analyze the effect of IVIG on rats in stress group. Results Sucrose consumption in IVIG group was significantly more than CUS group(P<0.01), while non floating time of force swim was significantly lower than CUS group(P<0.01). The difference of BrdU positive cell number in Hippocampus between two groups was significant(P<0.01). Conclusion IVIG show an antidepressant like effect, the mechanism may be involved in eliminate inflammatory cytokine of peripheral circulation.

Objective To investigate the characteristics of risk factors and coronary angiography (CAG)in young patients with acute myocardial infarction(AMI).Methods A total of 36 consecutive AMI female patients (age≤44 years)who underwent coronary angiography were retrospectively retrieved from the database,and 90 AMI patients whose age >44 years who also underwent coronary angiography were enrolled as a control (elderly)group.Characteristics of risk factors and coronary angiography were com-pared between the two groups.Results The risk factors:BMI,family history of coronary heart disease and high triglycerides(TG)were major in the youth group.The percentage of single-branch lesion was lower in the young patients.The prevalence of left anterior descending (LAD)was higher in the young patients(P <0.05).Conclusions The main risk factors in young and elderly patients are significantly different.The out-comes of CAG in the young patients show the coronary lesion are mostly single vessel lesion,and early inter-vention may improve prognosis.

Objective:To study the application of petrifilm colony count plate in the microbial detection for cosmetics. Methods:Three common microbials in 14 batches of cosmetics were respectively detected by the method described in hygienic standard for cos-metics and petrifilm colony count plate, and the results were compared. Results:The results shown by the two methods had no statisti-cally significant difference (P>0. 05). Conclusion: Petrifilm colony count plate with light weight, fast and easy operation exhibits significance in the microbial detection for cosmetics.

Objective To evaluate the effect of web-based teaching focused on the subject of history of nursing in the course of Introduction to Nursing. Method The 133 undergraduates in Class 2005 were randomly assigned to web-based teaching group and traditional teaching group focused on the subject of history of nursing in accordance with the same teach-ing objectives. The teaching outcomes were evaluated by a closed-book exam and questionnaire investigation. Results The students' performance in the exam was higher in the web-based teaching group than that of traditional teaching group(P<0.01). There were significant differences in the self-rated improvement of recognition and skills domains (P<0.05),while no significant difference in the affective domain (P=0.181). As a whole,the students' acceptance to web-based teaching was higher than the traditional teaching. Conclusion The web-based teaching can achieve good teaching outeomes,which can embody "student-centered" principle,improve the students' learning interest and initiative,and meet the teaching objectives in line with the re-quirements of quality education.

Objective To investigate the effects of hyperbaric oxygen (HBO) on ischemia/repeffusion (Ⅰ/R) injury of the small intestine after resuscitation from trauma and hemorrhagic shock (T/HS) in rats in order to elucidate the underlying mechanisms. Method Ninety-six male Wistar rats were randomly divided into four groups with 24 rats in each group. In shock group, rats were operated with induced T/HS. In sham group, rats operated without induced T/HS. In one HBO therapy (HBOT) group, rats with T/HS were treated with HBOT once. In three-HBOT group, rats with T/HS were treated with HBOT thrice. The Animal Care and Use Committee of China Medical University approved all animal protocols. Rats were anesthetized with amobarbital sodium (80 mg/kg, i.p.) at room temperature (25 ℃), the bloed pressure was monitored via polyethylene cannula inserted into the right femoral artery, connecting with the pressure analyzer (Multiparameter Monitor, M3046A, Boebin-gen, Germany). The left jugular vein was cannulated for administering normal saline and for resuscitation. The right carotid artery was cannulated for shedding blood. After operation, the middle part of left thigh of rat was se-lected as a site to be made of trauma by a lump of 2.5 kg iron falling upon from 20 cm height, causing the com-pound fracture of femur and crush injury of muscular tissue, then the damaged thigh was bandaged and fixed. At the same time, the blood was drawing out of fight carotid artery via cannula until the mean arterial was reduced to 30-35 mmHg within 5 minutes. The hypotension was kept constant for 60 minutes by additional drawing small amounts of blood as needed. After 60 minutes of hypotension, the rats were resuscitated by transfusing the shed blood over 5 minutes, followed by 4 -6 mL normal saline in 60 minutes to get the mean artery pressure maintained above 80 mmHg. The resuscitated rats were put into the hyperbaric chamber (10N-750, menoplace chamber, Ningho, China). The pressure inside the chamber was adjusted to 2.5 ATA and the oxygen concentration was higher than 95 %. The set pressure and oxygen concentration were maintained for 60 minutes. When the pressure within the chamber was decreased to 1 ATA, rots were taken out. The rats in one-HBOT group were given one HBO therapy immediately after resuscitation, and the rats in three-HBOT group were given one HBO therapy im-mediately after resuscitation with additional twice HBO therapy within 24 hours ((q 8 h). The one-way ANOVA and Pearson's bivariate methed were used for statistics. Results Twenty-four hours after resuscitation, the levels of lactate, induced nitric oxide synthase (iNOS), nitric oxide (NO), and tumor necrosis factor-α (TNF-α) in in-testinal tissue of rats in both HBOT groups were significantly lower than those in the rats of shock group without HBOT (P < 0.05). The histological injury grading scores of intestinal tissue in both HBOT group was significant lower than that in shock group (P < 0.05). There was positive correlation between the levels of iNOS and NO in intestinal tissue (P < 0.001). All the above-mentioned parameters in the three-HBOT group were better than those in one-HBOT group, but the difference was not significant (P > 0.05). Conclusions HBO decreases the production of inflammatory factors, inhibits the excessive inflammatory reaction to T/HS, and prevents the mucosal barrier of intestine from I/R injury after resuscitation from T/HS.

Objective To study the relationship between morphological and functional changes of melanocytes and their effect on the graft color. Methods Eighteen black whited guinea pigs were divided into nine groups randomly and biopsied at 1 d, 2 d, 3 d, 7 d, 14 d, 21 d, 28 d, and 90d after skin grafting, respectively. Then skin spliting and Dopa reaction techniques were used to demonstrate melanocytes. And computer assitant imagine analysis system was applied to observe and quantitate the morphological changes of melanocytes at various time points in three postoperative months. Results During three days after grafting, resulting from the surgery wound and shortage of blood and oxygen supply, the processes of the melanocytes broke off, accompanied by reduction of whole dimension of the cells, size as well as number of the processes. The indices above returned to the normal level between second week and third week after grafting. It was an evident characteristic that number of melanocytic processes became shorter and stouter in the end of the experiment. conclusion Functional changes of melanocytes in the skin grafts are in accord with their morphological changes. Compared to those in the sizes of melanocytes, changes in the processes of the cell are more sensitive in reflection of function of melanocytes. The shorter and stouter processes of melanocytes in the end of the experiment are suitable to the smaller epidermal melanin unit and more efficient in transformation of melanin into the keratinocytes.

Objective To know the drinking water quality in villages of Tongzhou District,Beijing and provide the basic data for government. Methods 623 wells in 455 villages were sampled in 2004 and the water samples were tested with the national standard methods. Results 166 of 623 water samples were up to standard,the qualification rate was 26.65%. The drinking water quality of all villages was different,in Maqiao(58.62%) and Liyuan(76.92%) it was better than that in Xiji,Huoxian,Zhangwan,Songzhuang. The qualification rate in Xiji was only 10.26%. In the present investigation,24 water quality parameters were tested and 14 parameters exceeded the limit,such as NH3-N,iron,total bacterial count,coliform group,fluoride and arsenic. Some sanitary problems were also found. Conclusion The ground water in Tongzhou has been contaminated,especially the shallow layer ground water,more attention must be paid to improvement of drinking water quality in these villages.

Objective To study the effect of pentoxifylline (PTX) on acute lung injury (ALI) in murine sepsis induced by intra abdominal infection. Methods Wistar rats were subjected to sepsis caused by cecal ligation and puncture (CLP), and animals were randomly divided into normal controls ( n =6), sepsis group ( n =18), and PTX treated group ( n =18). Pulmonary biopterin, nitric oxide (NO) levels, and pulmonary myeloperoxidase (MPO) activities were measured, guanosine triphosphate cyclohydrolase Ⅰ (GTP CHI), inducible nitric oxide synthase (iNOS), and tumor necrosis factor ? (TNF ?) mRNA expression were determined.Results Early treatment with PTX significantly decreased pulmonary MPO activities and TNF ?mRNA expression at 2 hours after CLP. Meanwhile, pulmonary biopterin, NO, GTP CHI, and iNOS mRNA expression levels were much lower in the treatment group than those in the non treatment group ( P