In order to improve the sterilizer operators' skills and reduce sterilization failure caused by wrong operations, the standardized training and examinations were provided for the sterilizer operators in accordance with their years of working ex-perience and professional title. The results indicated that the standardized training could improve the quality of sterilization, prolong the life of sterilizer and avoid sterilization failure due to wrong operations.

Objective To explore the effect of PDCA circulation on the effective utilization of the disposable articles in the operating room.Methods 9,362 aseptic articles for 446 operations in the departments of general surgery and hepatobiliary surgery during January to May 2012 were put in the pre-experiment group,in which PDCA circulation was not used.Another 11,973 articles for 501 operations in the same departments during June to November 2012 were set as the post-experiment group,in which PDCA circulation was used.The two groups were compared in terms of the effective utilization rate and the degree of doctor’s satisfaction with the nurses in the operation room.Result After the implementation of PDCA circulation,the effective utilization rate and the degree of doctor’s satisfaction were both significantly higher than before the implementation(both P<0.05).Conclusion PDCA circulation for the management of sterile disposable articles in the operation room may effectively improve the utilization rate of disposable articles, reduce wasting of articles and safe resources.

Objective To analyze the risk factors of the patients with gestational diabetes mellitus(GDM),to explore the effect of GDMon pregnancy outcome,and provide reference for early clinical prevention and intervention. Methods Randomly selected 220 GDMpatients who were treated from January 2014 to January 2016 as the observa-tion group,and collected 236 cases of non -diabetic pregnant women in our hospital during the same period as control group.Retrospectively analyzed the risk factors of GDM and the effect of GDM on pregnant women and neonates by questionnaires and clinical data.Results Single factor analysis and Logistic regression analysis showed that the age, history of diabetes,body mass index(BMI)before pregnancy,BMI before 1 week of childbirth and weight of oral glucose tolerance test(OGTT)were the risk factors of GDM(OR =2.988,5.684,4.255,3.765,3.051;P =0.003, 0.000,0.000,0.024,0.021 ).The incidences of phydramnion,postpartum hemorrhage,pregnancy period hyperten-sion,premature rupture,premature birth,cesarean section and ketoacidosis in the observation group were higher than the control group,and the differences were statistically significant (χ2 =5.539,11.338,14.103,4.527,8.160, 4.719;P =0.019,0.001,0.000,0.033,0.004,0.030).The incidence of newborn malformation,asphyxia,fetal distress and macrosomia in observation group were higher than the control group,and the differences were statistically significant(χ2 =4.929,5.539,4.163,5.950;P =0.026,0.019,0.041,0.015).Conclusion The age,history of diabetes,BMI before pregnancy,BMI before 1 week of childbirth and weight of OGTT were the risk factors of GDM, which should pay close attention to these people and take corresponding treatment measures,so as to improve the adverse outcome of maternal and infant in clinic.

Objective To investigate the effect of labor induction of full-term pregnancy use of oxytocin,and the incidence of adverse reactions of mode of delivery of cervical dilatation balloon with pubeisheng.Methods The clinical data of full-term pregnant women who were delivered in our hospital from October 2014 to November 2015 were retrospectively analyzed,According to the induction of labor is divided into the way of pubeisheng group and cervical dilation balloon group.The differences of delivery mode,oxytocin utilization,maternal and neonatal outcomes and adverse reactions were observed between the two groups.Results Cervical dilatation balloon group vaginal delivery rate was 90.38％,higher than that of pubeisheng group(P<0.05); oxytocin use rate of 11.54％,lower than that of pubeisheng group(P<0.05); Cervical dilatation balloon group first,second and third labor time is shorter pubeisheng group(P<0.001); The cervical dilation balloon group bleeding more than pubeisheng group(P<0.001),two groups of infection,urinary retention complication rate had no significant difference.Two groups of neonatal aspiration pneumonia incidence rate had no significant difference after the birth of 1min Apgar score difference,and cervical dilatation balloon group after the birth of 5min Apgar was higher than that of pubeisheng group(P<0.05).Conclusion The application of cervical dilation balloon in the induction of labor in term pregnancy is better,which can significantly improve the rate of vaginal delivery,reduce the use rate of oxytocin and shorten the labor process,and has the value of clinical application.

Salvia miltorrhiza Bge is a medicinal herb in traditional Chinese medicines. Until recently, dozens of compound ingredients with medicinal properties have been identified from S. miltorrhiza. Proteome analysis on medicinal ingredients synthesis mechanisms could provide a theoretical basis for S. miltorrhiza genetic improve-ment. In this study, a S. miltorrhiza specific protein/peptide sequence database was constructed using Illumina high-throughput transcriptome sequencing data of multiple types of S. miltorrhiza tissues. The database could act as a key component to carry out the proteome analysis in S. miltorrhiza.

OBJECTIVE:To investigate the efficacy and safety of carvedilol in patients with chronic heart failure(CHF). METHODS:46patients with CHF were randomly divided into carvedilol group(n=26)and control group(n=20),thareinto,standard therapeutic scheme is used in control group,standard therapeutic scheme+carvedilol is used in carve diol greup.The treatment courses were6months in both groups.RESULTS:Left ventricular end-diastolic diameter(LVEDD)reduced and left ventricular ejection fraction(LVEF)increased in carvedilol group with significant difference comparing with control group(P

Objective To discuss the effect of syphilis treatment during pregnancy on serological test results in infants born to syphilis mothers.Methods Data of pregnant women with syphilis and their infants in Zhejiang Province between January 2013 and December 2014 were retrospectively analyzed and the effect of syphilis treatment during pregnancy on non-treponemal antigen serological test results in infants were compared.x2 test was used for positive rate of serological tests,incidence of congenital syphilis,and neonatal mortality in infants with syphilis,and rank sum test was used to compare the titers of rapid plasma reagin (RPR) test and tolulized red unheated serum teat (TRUST).Results A total of 3 953 infants were born to 3 901 mothers with syphilis.Of which 3 262 were tested,including 2 226 by RPR and 1 036 by TRUST.The rate of infants with positive RPR was significant lower in treated mothers (45.02％)compared with untreated mothers (55.04％;x2 =12.88,P＜0.01).The significant differences of titers of RPR or TRUST were observed among mothers with or without treatment (RPR:Z=-2.45,P=0.01;TRUST:Z=-2.65,P=0.01),different course of treatment (RPR:Z=-2.33,P=0.02;TRUST:Z=-2.60,P=0.01).Fifty-seven infants were diagnosed with congenital syphilis.The rate of congenital syphilis was 3.50％(27/772) born to untreated mothers,which was dramatically higher than treated mothers (0.96 ％,30/3 129,x2 =27.72,P＜ 0.01).Forty-two infants died.Neonatal mortality was also higher in the untreated group (2.59 ％,20/772) than the treated group (0.70％,22/3 129,x2 =20.72,P＜ 0.01).Conclusion Adequate treatment for women with syphilis during pregnancy could decrease serological tests of RPR and TRUST among new infants and improve the infants their outcomes.

Objective To establish a reliable unstable pelvic fracture model with sacral plexus stretch injury in goats and to detect electrophysiological signals when pelvis is shifted in different orientations and distance.Methods Twenty-four goats were divided into three groups of 8 animals each:pelvic longitudinal displacement was induced in group A,pelvic lateral displacement in group B,and pelvic dorsal displacement in group C.Animal left L6 and S1 nerve root and initial segment of sciatic nerve were exposed to connect stimulating and receiving electrodes respectively.Left pelvis was shifted at the speed of 0.25 mm/s to produce the model of unstable pelvic fracture with sacral plexus stretch injury.Electrophysiological signals of L6 and S1 were recorded when the placement of pelvis was 0,0.5 and 1.0 cm respectively.Results With pelvic displacement,the threshold potential,maximum stimulus potential and incubation period were gradually increased,but the peak-to-peak value became smaller gradually.When the pelvis displaced 0.5 cm at different orientations,group A showed improved threshold potential and incubation period (P ＜0.05),decreased peak-to-peak value (P ＜0.05),and no significant change in maximum stimulus potential(P ＞0.05);group B only showed lowered peak-to-peak value with no significant variations in other electrophysiological signals (P ＜ 0.05);group C had no significant variations in all electrophysiological signals (P ＞ 0.05).When the pelvis displaced 1.0 cm at different orientations,groups A and B showed improved threshold potential,maximum stimulus potential and incubation period (P ＜ 0.05) and lowered peak to peak value (P ＜ 0.05);group C showed higher incubation period with no significant variations in other electrophysiological signals(P ＜0.05).Between groups,the amplitude of variation in electrophysiological signals was the most in group A and the least in group C.Conclusion After unstable pelvis fracture in goats,sacral plexus stretch injury is the most serious in longitudinal displacement of the pelvis.

Objective With the isocentric C-arm (Iso-C) three-dimensional computerized navigation system,cadaveric pelvic specimens were used to imitate double screw fixation of the symphysis pubic.Practicability and safety of the screw trajectory were examined postoperatively by local cadaveric dissections and imaging tests.Methods Pelvic specimens were harvested from 8 male and 7 female adult cadavers.Double screw placement in symphysis pubic was performed using the Iso-C three-dimensional navigation and entry point and safety trajectory was achieved.With the detailed local dissection postoperatively,distances from screw entry and exit points to unilateral structures (spermatic cord,femoral artery and vein,femoral nerve,obturator artery and vein,obtrurator nerve,and so on) were measured respectively.After complete removal of surrounding soft tissues of the specimen with only bony structure kept,the entry angle and length were calculated.Accuracy of double screw fixation of symphysis pubic was further checked using X-ray and CT.Results Entry point of the first screw was at the junction of unilateral pubic tubercle and transitional site of superior pubic ramus.Mean angle of the first screw with the horizontal plane was (7.7 ± 1.9) ° in men and (8.1 ± 1.7) ° in women.Mean angle between the first screw and coronal plane was (7.8 ± 1.8)° in men and (7.7 ±2.0)° in women.Entry point of the second screw was in the same place in the contralateral pubic tubercle.Mean angle between the second screw and horizontal plane was (30.6 ± 4.0) ° in men and (30.8 ± 3.4) ° in women.Mean angle between the second screw and coronal plane was (9.1 ± 3.0)°in men and (9.2 ± 3.3)°in women.Conclusions With the three-dimensional computerized navigation system,the bony channels of double screws implanted in the symphysis pubic are achieved and reliable.Percutaneous double screw fixation is feasible to treat the pubic symphysis diastasis.

Objective To explore the status of social support and its relationship with depression in elderly university students.Methods 159 students aged 55 to 79 years of Nanning elderly university were selected using cluster sampling methods.They were surveyed by Social Support Rating Scale and Geriatric Depression Scale.Results ①The total score of social support(40.92±7.63) was higher than that of the national norm(34.56±3.73 ) (t=10.515,P＜0.01),and score of social support,subjective support,utilization of support were higher than those of the elderly in Beijing Xicheng district community that was mental health guidance demonstration area in national community (t=2.064,2.017,9.222; P＜0.05).②Social support score and each dimension score of having a spouse group were higher than those of no spouse group (t=5.78,4.956,4.918,3.175 ; P＜0.0 1).③The positive rate of depression in the sample group was 26.8％.Social support score,objective support score,subjective support score of depression in positive group(37.43±7.41,21.43±4.93,7.67±2.37) were lower than those of negative group(42.17 ±7.35,24.43±4.22,8.78±2.83,t=3.585,2.277,3.776 ; P＜0.05).④Social support,objective support,subjective support and depression were negatively correlated (r=-0.296,-0.194,-0.307; P＜0.01).Conclusion Elderly university student's overall level of social support are higher,and social support is negatively correlated with depressive symptoms.